Micronucleus formation in mussel’ (Mytilus galloprovincialis, Lamarck 1819) haemolymph, liver, and gill cells as a biomarker in the assessment of genotoxicity in Izmir Bay (Aegean Sea, Turkey)

Özlem Çakal Arslan; Meltem Boyacıoglu; Beyza Nalbantlar; Gizem Gülsever; Muhammet Ali Karaaslan

doi:10.12714/egejfas.38.2.08

TR EN

Izmir Körfezi (Ege Denizi, Türkiye) genotoksisitenin değerlendirilmesinde biyobelirteç olarak Midye (Mytilus galloprovincialis) hemolenf, karaciğer ve solungaç hücrelerinde mikronükleus oluşumu

Öz

Denizler, çok çeşitli çevresel kirleticileri içermekte ve su ekosistemlerinde önemli bir rol oynamaktadır. 1960'lı yıllardan itibaren hızla kirlenen İzmir Körfezi, Akdeniz'deki kirliliğin en yoğun olduğu alanlardan biridir. Bölgede biriken organik maddeler, hidrokarbonlar, metaller ve patojen organizmalar, estetik ve sağlık açısından yüksek derecede tehdit oluşturmaktadır. Bu nedenle, bu çalışmanın amacı İzmir Körfezi’ nde bulunan midyelerde meydana gelen genetik hasarların araştırılmasına odaklanmıştır. Mutajenik / karsinojenik potansiyelin araştırılması, genotoksisite çalışmalarında bir avantaj sağlamaktadır, çünkü kirliliğe maruz kalan midyelerde kullanılan, biyobelirteçler; kanser gibi olası uzun vadeli etkilerin erken saptanmasına olanak sağlamaktadır. Biyoizleme çalışmalarında; midyede yapılan biyobelirteçlerin merkezinde genotoksisite bulunmaktadır. Bu amaçla çalışmamızda; İzmir Körfezi'nde (Türkiye'nin Batı Sahili) yaşayan Mytilus galloprovincialis'in hemolenf hücrelerinde, karaciğer hücrelerinde ve solungaç hücrelerinde genetik hasarı belirlemek için mikronükleus testleri gerçekleştirilmiştir. Bu çalışmada elde edilen sonuçlar, tersaneden gelen atıkların bulunduğu 4. istasyon ve 5. istasyonda MN frekansının (‰ micronucleus) yüksek bulunmasına paralel olarak, kirliliğin bu bölgelerde yoğun olduğunu göstermiştir. Elde ettiğimiz verilerle, çevresel kirliliğin biyolojik olarak izlenmesi için, kirleticilerin suda yaşayan türlerde hücresel düzeyde toksik etkilerinin belirlenebilmesini, mikronükleus testlerinin kullanımının yaygınlaştırılmasını ve bu yöntemlerin standartlaştırılmasının gerekliliğini ortaya koymaktayız

Anahtar Kelimeler

Micronucleus formation in mussel’ (Mytilus galloprovincialis, Lamarck 1819) haemolymph, liver, and gill cells as a biomarker in the assessment of genotoxicity in Izmir Bay (Aegean Sea, Turkey)

Abstract

The sea has involved a large variety of environmental contaminants and plays a crucial role in aquatic ecosystems. The Izmir Bay, which has been rapidly polluted since the 1960s, was one of the intensely polluted areas in the Mediterranean. Organic materials, hydrocarbons, metals, and pathogenic organisms that are accumulated in the region, caused high pollution and threaten health and aesthetics. Because of this, the aim of this paper was focus on to investigations of the genetic damages in mussels in Izmir Bay. Investigations of mutagenic/carcinogenic potential have an advantage in genotoxicity studies because biomarker for pollution exposure in mussel is the early detection of possible long-term effects such as cancer. Therefore, genotoxicity was the focus of the biomarker investigations in mussel during the investigations. Thus, micronucleus tests were afforded to determine genetic damage in the haemolymph, liver, and gills of Mytilus galloprovincialis living in Izmir Bay (Western Coast of Turkey). In the present study, results showed that the frequency of MN was found at a high level in station 4 and station 5 where wastes from dockyard existed contributed to the high level of pollution. The recommendation is to standardize procedures for assessment of the toxic impact of pollutants at the cellular level in aquatic species by using micronucleus assays for biomonitoring of environmental pollution.

Keywords

Destekleyen Kurum

Ege Üniversitesi, Su Ürünleri Fakültesi

Proje Numarası

2016/SÜF/006

Teşekkür

The present study was supported in the context of the Scientific Research Project of Ege University Faculty of Fisheries. Hydrobiology Department (Project No: 2016/SÜF/006).

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Hidrobiyoloji , Çevre Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Özlem Çakal Arslan ^*
0000-0001-7777-3886
Türkiye

Meltem Boyacıoglu
0000-0002-9871-6132
Türkiye

Beyza Nalbantlar
0000-0003-2206-9589
Türkiye

Gizem Gülsever
0000-0001-7526-6207
Türkiye

Muhammet Ali Karaaslan
0000-0003-3737-2361
Türkiye

Yayımlanma Tarihi

15 Haziran 2021

Gönderilme Tarihi

2 Ekim 2020

Kabul Tarihi

1 Şubat 2021

Yayımlandığı Sayı

Yıl 2021 Cilt: 38 Sayı: 2

DOI

https://doi.org/10.12714/egejfas.38.2.08

IZ

https://izlik.org/JA97PC64GY

Kaynak Göster

RIS / Bibtex

APA

Çakal Arslan, Ö., Boyacıoglu, M., Nalbantlar, B., Gülsever, G., & Karaaslan, M. A. (2021). Micronucleus formation in mussel’ (Mytilus galloprovincialis, Lamarck 1819) haemolymph, liver, and gill cells as a biomarker in the assessment of genotoxicity in Izmir Bay (Aegean Sea, Turkey). Ege Journal of Fisheries and Aquatic Sciences, 38(2), 189-197. https://doi.org/10.12714/egejfas.38.2.08

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