Ege Journal of Fisheries and Aquatic Sciences
Research Article
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Micronucleus formation in mussel’ (Mytilus galloprovincialis, Lamarck 1819) haemolymph, liver, and gill cells as a biomarker in the assessment of genotoxicity in Izmir Bay (Aegean Sea, Turkey)

Year 2021, Volume: 38 Issue: 2, 189 - 197, 15.06.2021

Özlem ÇAKAL ARSLAN Meltem BOYACIOGLU Beyza NALBANTLAR Gizem GÜLSEVER Muhammet Ali KARAASLAN

https://doi.org/10.12714/egejfas.38.2.08

Abstract


The sea has involved a large variety of environmental contaminants and plays a crucial role in aquatic ecosystems. The Izmir Bay, which has been rapidly polluted since the 1960s, was one of the intensely polluted areas in the Mediterranean. Organic materials, hydrocarbons, metals, and pathogenic organisms that are accumulated in the region, caused high pollution and threaten health and aesthetics. Because of this, the aim of this paper was focus on to investigations of the genetic damages in mussels in Izmir Bay. Investigations of mutagenic/carcinogenic potential have an advantage in genotoxicity studies because biomarker for pollution exposure in mussel is the early detection of possible long-term effects such as cancer. Therefore, genotoxicity was the focus of the biomarker investigations in mussel during the investigations. Thus, micronucleus tests were afforded to determine genetic damage in the haemolymph, liver, and gills of Mytilus galloprovincialis living in Izmir Bay (Western Coast of Turkey). In the present study, results showed that the frequency of MN was found at a high level in station 4 and station 5 where wastes from dockyard existed contributed to the high level of pollution. The recommendation is to standardize procedures for assessment of the toxic impact of pollutants at the cellular level in aquatic species by using micronucleus assays for biomonitoring of environmental pollution.

Keywords

micronucleus test, pollution, genotoxicity, mussel

Supporting Institution

Ege Üniversitesi, Su Ürünleri Fakültesi

Thanks

The present study was supported in the context of the Scientific Research Project of Ege University Faculty of Fisheries. Hydrobiology Department (Project No: 2016/SÜF/006).

References

  • Arslan, Ö., Parlak, H., Katalay, S., Boyacioglu, M., Karaaslan, M. A., & Guner, H. (2010). Detecting micronuclei frequency in some aquatic organisms for monitoring pollution of Izmir Bay (Western Turkey). Environmental Monitoring and Assessment, 165(1–4), 55–66. DOI:10.1007/s10661-009-0926-5
  • Arslan, Ö., Boyacioğlu, M., Parlak, H., Katalay, S. & Karaaslan, M. A. (2015). Assessment of micronuclei induction in peripheral blood and gill cells of some fish species from Aliağa Bay Turkey. Marine Pollution Bulletin, 94(1–2),48–54. DOI:10.1016/j.marpolbul.2015.03.018
  • Barsiene, J., Schiedek, D., Rybakovas, A., Syvokiene, J., Kopecka, J., & Förlin, L. (2006). Cytogenetic and cytotoxic effects in gill cells of the blue mussel Mytilus spp. from different zones of the Baltic Sea. Marine Pollution Bulletin, 53(8–9), 469–478. DOI:10.1016/j.marpolbul.2005.11.015
  • Bolognesi, C. & Hayashi, M. (2011). Micronucleus assay in aquatic animals. Mutagenesis, 26(1), 205–213. DOI:10.1093/mutage/geq073
  • Bolognesi, C. & Fenech, M. (2012). Mussel micronucleus cytome assay. Nature Protocols, 7(6), 1125–1137. DOI:10.1038/nprot.2012.043
  • Bolognesi, C. & Cirillo, S. (2014). Genotoxicity biomarkers in aquatic bioindicators. Current Zoology, 60(2), 273–284. DOI:10.1093/czoolo/60.2.273
  • Boyacıoğlu, M., & Parlak, H. (2001). Mutagenicity of River Sediments Draining into Izmir Bay. Ege Journal of Fisheries and Aquatic Sciences, 18(3), 23-36.
  • Boyacıoğlu, M. (2004). Determination of direct mutagens in sediment samples of Izmir Bay. Ege Journal of Fisheries and Aquatic Sciences, 21(1), 11-18.
  • Dailianis, S., Domouhtsidou, G. P., Raftopoulou, E., Kaloyianni, M. & Dimitriadis, V. K. (2003). Evaluation of neutral red retention assay, micronucleus test, acetylcholinesterase activity, and a signal transduction molecule (cAMP) in tissues of Mytilus galloprovincialis (L.), in pollution monitoring. Marine Environmental Research, 56(4), 443–470. DOI:10.1016/S0141-1136(03)00005-9
  • Dolcetti, L. & Venier, P. (2002). Susceptibility to genetic damage and cell types in Mediterranean mussels. Marine Environmental Research, 54(3–5), 487–491. DOI:10.1016/S0141-1136(02)00142-3
  • Fenech, M. (2000). The in vitro micronucleus technique. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 455(1–2), 81–95. DOI: 10.1016/S0027-5107(00)00065-8
  • Gulsever G. & Çakal Arslan O. (2019). İzmir iç körfezi sediment kirliliğinin kimyasal ve biyolojik entegre yöntemler ile incelenmesi. 215 sayfa, Doktora Tezi, E.Ü Fen Bilimleri Enstitüsü, İzmir.
  • Harvey, J. S., Lyons, B. P., Page, T. S., Stewart, C. & Parry, J. M. (1999). An assessment of the genotoxic impact of the Sea Empress oil spill by the measurement of DNA adduct levels in selected invertebrate and vertebrate species. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 441(1), 103-114. DOI: 10.1016/S1383-5718(99)00037-6
  • Hayashi, M., Ueda, T., Uyeno, K., Wada, K., Kinae, N., Saotome, K.& Ojima, Y. (1998). Development of genotoxicity assay systems that use aquatic organisms. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 399(2), 125-133. DOI: 10.1016/S0027-5107(97)00251-0
  • Izquierdo, J. I., Machado, G., Ayllon, F., d’Amico, V. L., Bala, L. O., Vallarino, E. & Garcia-Vazquez, E. (2003). Assessing pollution in coastal ecosystems: a preliminary survey using the micronucleus test in the mussel Mytilus edulis. Ecotoxicology and Environmental Safety, 55(1), 24-29. DOI: 0.1016/S0147-6513(02)00041-6
  • Kalpaxis, D. L., Theos, C., Xaplanteri, M. A., Dinos, G. P., Catsiki, A. V. & Leotsinidis, M. (2004). Biomonitoring of Gulf of Patras, N. Peloponnesus, Greece. Application of a biomarker suite including evaluation of translation efficiency in Mytilus galloprovincialis cells. Environmental Research, 94(2), 211–220. DOI:10.1016/S0013-9351(03)00048-3
  • Klobučar, G. I. V., Štambuk, A., Hylland, K. & Pavlica, M. (2008). Detection of DNA damage in haemocytes of Mytilus galloprovincialis in the coastal ecosystems of Kaštela and Trogir bays, Croatia. Science of the Total Environment, 405(1–3), 330–337. DOI:10.1016/j.scitotenv.2008.05.015
  • Kontas, A., Kucuksezgin, F., Altay, O. & Uluturhan, E. (2004). Monitoring of eutrophication and nutrient limitation in the Izmir Bay (Turkey) before and after Wastewater Treatment Plant. Environment International, 29(8), 1057–1062. DOI:10.1016/S0160-4120(03)00098-9
  • Magni, P., De Falco, G., Falugi, C., Franzoni, M., Monteverde, M., Perrone, E. & Bolognesi, C. (2006). Genotoxicity biomarkers and acetylcholinesterase activity in natural populations of Mytilus galloprovincialis along a pollution gradient in the Gulf of Oristano (Sardinia, western Mediterranean). Environmental Pollution, 142(1), 65–72. DOI:10.1016/j.envpol.2005.09.018
  • Sinem Atgin, R., El-Agha, O., Zararsiz, A., Kocataş, A., Parlak, H. & Tuncel, G. (2000). Investigation of the sediment pollution in Izmir Bay: trace elements. Spectrochimica Acta, Part B: Atomic Spectroscopy, 55(7), 1151–1164. DOI:10.1016/S0584-8547(00)00231-7
  • Touahri, H. G., Boutiba, Z., Benguedda, W. & Shaposhnikov, S. (2016). Active biomonitoring of mussels Mytilus galloprovincialis with integrated use of micronucleus assay and physiological indices to assess harbor pollution. Marine Pollution Bulletin, 110(1), 52-64 . Tsarpali, V. & Dailianis, S. (2012). Investigation of landfill leachate toxic potency: An integrated approach with the use of stress indices in tissues of mussels. Aquatic toxicology, 124, 58-65. DOI:10.1016/j.aquatox.2012.07.008
  • Vahl, H. H., Karbe, L. & Westendorf, J. (1997). Genotoxicity assessment of suspended particulate matter in the Elbe river: Comparison of Salmonella microsome test, arabinose resistance test, and umu-test. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 394(1–3), 81–93. DOI:10.1016/S1383-5718(97)00129-0
  • Venier, P., & Zampieron, C. (2005). Evidence of genetic damage in grass gobies and mussels from the Venice lagoon. Environment International, 31(7), 1053-1064. DOI:10.1016/j.envint.2005.05.016

Izmir Körfezi (Ege Denizi, Türkiye) genotoksisitenin değerlendirilmesinde biyobelirteç olarak Midye (Mytilus galloprovincialis) hemolenf, karaciğer ve solungaç hücrelerinde mikronükleus oluşumu

Year 2021, Volume: 38 Issue: 2, 189 - 197, 15.06.2021

Özlem ÇAKAL ARSLAN Meltem BOYACIOGLU Beyza NALBANTLAR Gizem GÜLSEVER Muhammet Ali KARAASLAN

https://doi.org/10.12714/egejfas.38.2.08

Abstract


Denizler, çok çeşitli çevresel kirleticileri içermekte ve su ekosistemlerinde önemli bir rol oynamaktadır. 1960'lı yıllardan itibaren hızla kirlenen İzmir Körfezi, Akdeniz'deki kirliliğin en yoğun olduğu alanlardan biridir. Bölgede biriken organik maddeler, hidrokarbonlar, metaller ve patojen organizmalar, estetik ve sağlık açısından yüksek derecede tehdit oluşturmaktadır. Bu nedenle, bu çalışmanın amacı İzmir Körfezi’ nde bulunan midyelerde meydana gelen genetik hasarların araştırılmasına odaklanmıştır. Mutajenik / karsinojenik potansiyelin araştırılması, genotoksisite çalışmalarında bir avantaj sağlamaktadır, çünkü kirliliğe maruz kalan midyelerde kullanılan, biyobelirteçler; kanser gibi olası uzun vadeli etkilerin erken saptanmasına olanak sağlamaktadır. Biyoizleme çalışmalarında; midyede yapılan biyobelirteçlerin merkezinde genotoksisite bulunmaktadır. Bu amaçla çalışmamızda; İzmir Körfezi'nde (Türkiye'nin Batı Sahili) yaşayan Mytilus galloprovincialis'in hemolenf hücrelerinde, karaciğer hücrelerinde ve solungaç hücrelerinde genetik hasarı belirlemek için mikronükleus testleri gerçekleştirilmiştir. Bu çalışmada elde edilen sonuçlar, tersaneden gelen atıkların bulunduğu 4. istasyon ve 5. istasyonda MN frekansının (‰ micronucleus) yüksek bulunmasına paralel olarak, kirliliğin bu bölgelerde yoğun olduğunu göstermiştir. Elde ettiğimiz verilerle, çevresel kirliliğin biyolojik olarak izlenmesi için, kirleticilerin suda yaşayan türlerde hücresel düzeyde toksik etkilerinin belirlenebilmesini, mikronükleus testlerinin kullanımının yaygınlaştırılmasını ve bu yöntemlerin standartlaştırılmasının gerekliliğini ortaya koymaktayız 

Keywords

mikronükleus testi, kirlilik, genotoksisite, midye

References

  • Arslan, Ö., Parlak, H., Katalay, S., Boyacioglu, M., Karaaslan, M. A., & Guner, H. (2010). Detecting micronuclei frequency in some aquatic organisms for monitoring pollution of Izmir Bay (Western Turkey). Environmental Monitoring and Assessment, 165(1–4), 55–66. DOI:10.1007/s10661-009-0926-5
  • Arslan, Ö., Boyacioğlu, M., Parlak, H., Katalay, S. & Karaaslan, M. A. (2015). Assessment of micronuclei induction in peripheral blood and gill cells of some fish species from Aliağa Bay Turkey. Marine Pollution Bulletin, 94(1–2),48–54. DOI:10.1016/j.marpolbul.2015.03.018
  • Barsiene, J., Schiedek, D., Rybakovas, A., Syvokiene, J., Kopecka, J., & Förlin, L. (2006). Cytogenetic and cytotoxic effects in gill cells of the blue mussel Mytilus spp. from different zones of the Baltic Sea. Marine Pollution Bulletin, 53(8–9), 469–478. DOI:10.1016/j.marpolbul.2005.11.015
  • Bolognesi, C. & Hayashi, M. (2011). Micronucleus assay in aquatic animals. Mutagenesis, 26(1), 205–213. DOI:10.1093/mutage/geq073
  • Bolognesi, C. & Fenech, M. (2012). Mussel micronucleus cytome assay. Nature Protocols, 7(6), 1125–1137. DOI:10.1038/nprot.2012.043
  • Bolognesi, C. & Cirillo, S. (2014). Genotoxicity biomarkers in aquatic bioindicators. Current Zoology, 60(2), 273–284. DOI:10.1093/czoolo/60.2.273
  • Boyacıoğlu, M., & Parlak, H. (2001). Mutagenicity of River Sediments Draining into Izmir Bay. Ege Journal of Fisheries and Aquatic Sciences, 18(3), 23-36.
  • Boyacıoğlu, M. (2004). Determination of direct mutagens in sediment samples of Izmir Bay. Ege Journal of Fisheries and Aquatic Sciences, 21(1), 11-18.
  • Dailianis, S., Domouhtsidou, G. P., Raftopoulou, E., Kaloyianni, M. & Dimitriadis, V. K. (2003). Evaluation of neutral red retention assay, micronucleus test, acetylcholinesterase activity, and a signal transduction molecule (cAMP) in tissues of Mytilus galloprovincialis (L.), in pollution monitoring. Marine Environmental Research, 56(4), 443–470. DOI:10.1016/S0141-1136(03)00005-9
  • Dolcetti, L. & Venier, P. (2002). Susceptibility to genetic damage and cell types in Mediterranean mussels. Marine Environmental Research, 54(3–5), 487–491. DOI:10.1016/S0141-1136(02)00142-3
  • Fenech, M. (2000). The in vitro micronucleus technique. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 455(1–2), 81–95. DOI: 10.1016/S0027-5107(00)00065-8
  • Gulsever G. & Çakal Arslan O. (2019). İzmir iç körfezi sediment kirliliğinin kimyasal ve biyolojik entegre yöntemler ile incelenmesi. 215 sayfa, Doktora Tezi, E.Ü Fen Bilimleri Enstitüsü, İzmir.
  • Harvey, J. S., Lyons, B. P., Page, T. S., Stewart, C. & Parry, J. M. (1999). An assessment of the genotoxic impact of the Sea Empress oil spill by the measurement of DNA adduct levels in selected invertebrate and vertebrate species. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 441(1), 103-114. DOI: 10.1016/S1383-5718(99)00037-6
  • Hayashi, M., Ueda, T., Uyeno, K., Wada, K., Kinae, N., Saotome, K.& Ojima, Y. (1998). Development of genotoxicity assay systems that use aquatic organisms. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 399(2), 125-133. DOI: 10.1016/S0027-5107(97)00251-0
  • Izquierdo, J. I., Machado, G., Ayllon, F., d’Amico, V. L., Bala, L. O., Vallarino, E. & Garcia-Vazquez, E. (2003). Assessing pollution in coastal ecosystems: a preliminary survey using the micronucleus test in the mussel Mytilus edulis. Ecotoxicology and Environmental Safety, 55(1), 24-29. DOI: 0.1016/S0147-6513(02)00041-6
  • Kalpaxis, D. L., Theos, C., Xaplanteri, M. A., Dinos, G. P., Catsiki, A. V. & Leotsinidis, M. (2004). Biomonitoring of Gulf of Patras, N. Peloponnesus, Greece. Application of a biomarker suite including evaluation of translation efficiency in Mytilus galloprovincialis cells. Environmental Research, 94(2), 211–220. DOI:10.1016/S0013-9351(03)00048-3
  • Klobučar, G. I. V., Štambuk, A., Hylland, K. & Pavlica, M. (2008). Detection of DNA damage in haemocytes of Mytilus galloprovincialis in the coastal ecosystems of Kaštela and Trogir bays, Croatia. Science of the Total Environment, 405(1–3), 330–337. DOI:10.1016/j.scitotenv.2008.05.015
  • Kontas, A., Kucuksezgin, F., Altay, O. & Uluturhan, E. (2004). Monitoring of eutrophication and nutrient limitation in the Izmir Bay (Turkey) before and after Wastewater Treatment Plant. Environment International, 29(8), 1057–1062. DOI:10.1016/S0160-4120(03)00098-9
  • Magni, P., De Falco, G., Falugi, C., Franzoni, M., Monteverde, M., Perrone, E. & Bolognesi, C. (2006). Genotoxicity biomarkers and acetylcholinesterase activity in natural populations of Mytilus galloprovincialis along a pollution gradient in the Gulf of Oristano (Sardinia, western Mediterranean). Environmental Pollution, 142(1), 65–72. DOI:10.1016/j.envpol.2005.09.018
  • Sinem Atgin, R., El-Agha, O., Zararsiz, A., Kocataş, A., Parlak, H. & Tuncel, G. (2000). Investigation of the sediment pollution in Izmir Bay: trace elements. Spectrochimica Acta, Part B: Atomic Spectroscopy, 55(7), 1151–1164. DOI:10.1016/S0584-8547(00)00231-7
  • Touahri, H. G., Boutiba, Z., Benguedda, W. & Shaposhnikov, S. (2016). Active biomonitoring of mussels Mytilus galloprovincialis with integrated use of micronucleus assay and physiological indices to assess harbor pollution. Marine Pollution Bulletin, 110(1), 52-64 . Tsarpali, V. & Dailianis, S. (2012). Investigation of landfill leachate toxic potency: An integrated approach with the use of stress indices in tissues of mussels. Aquatic toxicology, 124, 58-65. DOI:10.1016/j.aquatox.2012.07.008
  • Vahl, H. H., Karbe, L. & Westendorf, J. (1997). Genotoxicity assessment of suspended particulate matter in the Elbe river: Comparison of Salmonella microsome test, arabinose resistance test, and umu-test. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 394(1–3), 81–93. DOI:10.1016/S1383-5718(97)00129-0
  • Venier, P., & Zampieron, C. (2005). Evidence of genetic damage in grass gobies and mussels from the Venice lagoon. Environment International, 31(7), 1053-1064. DOI:10.1016/j.envint.2005.05.016

Details

Primary Language English
Subjects Hydrobiology, Environmental Sciences
Journal Section Articles
Authors

Özlem ÇAKAL ARSLAN
ege üniversitesi
0000-0001-7777-3886
Türkiye

Meltem BOYACIOGLU
EGE ÜNİVERSİTESİ
0000-0002-9871-6132
Türkiye

Beyza NALBANTLAR
EGE ÜNİVERSİTESİ
0000-0003-2206-9589
Türkiye

Gizem GÜLSEVER
EGE ÜNİVERSİTESİ
0000-0001-7526-6207
Türkiye

Muhammet Ali KARAASLAN
EGE ÜNİVERSİTESİ
0000-0003-3737-2361
Türkiye

Project Number 2016/SÜF/006
Publication Date June 15, 2021
Submission Date October 2, 2020
Acceptance Date February 1, 2021
Published in Issue Year 2021Volume: 38 Issue: 2

Cite

APA Çakal Arslan, Ö. , Boyacıoglu, M. , Nalbantlar, B. , Gülsever, G. & Karaaslan, M. A. (2021). Micronucleus formation in mussel’ (Mytilus galloprovincialis, Lamarck 1819) haemolymph, liver, and gill cells as a biomarker in the assessment of genotoxicity in Izmir Bay (Aegean Sea, Turkey) . Ege Journal of Fisheries and Aquatic Sciences , 38 (2) , 189-197 . DOI: 10.12714/egejfas.38.2.08