Araştırma Makalesi

Identification, characterization and draft genome analysis of Vibrio anguillarum VK1 isolated from hatchery water

Cilt: 43 Sayı: 3 14 Haziran 2026
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Identification, characterization and draft genome analysis of Vibrio anguillarum VK1 isolated from hatchery water

Öz

Aquaculture environments are dynamic ecosystems that may influence the evolution and adaptive potential of bacterial pathogens. In this study, a locally isolated Vibrio anguillarum  strain VK1 (PRJNA1452808) from Türkiye was characterized using 16S rDNA phylogenetic analysis and whole-genome-based approaches. The high-quality draft genome (4.23 Mb; GC content 44.31%) comprised 194 contigs and 4,102 coding sequences, including 1,057 hypothetical proteins. Functional analyses revealed putative stress response mechanisms and iron acquisition systems, suggesting metabolic versatility and environmental adaptability. Genome-based screening identified 33 putative antimicrobial resistance-associated determinants, including multidrug efflux-related genes and genes linked to antibiotics commonly used in aquaculture. In addition, genes associated with Type III and Type VI secretion systems were detected, potentially indicating adaptive traits related to host interaction and environmental persistence. Overall, the findings suggest that strain VK1 possesses a flexible genomic structure that may support adaptation to fluctuating aquaculture conditions. However, further functional studies are needed to biologically validate the potential virulence and AMR related features.

Anahtar Kelimeler

Destekleyen Kurum

Ege University

Proje Numarası

23352

Etik Beyan

This study does not involve human participants or animal experiments.

Teşekkür

The authors would like to express their gratitude to the staff of Kılıç Seafood Inc. at the Didim Incubation and Adaptation Facility for their support. This work is part of Kadriye Toklu’s master’s thesis and was substantially supported by the Ege University Scientific Research Projects Coordination Office (Grant No. 23352).

Kaynakça

  1. AlJindan, R., AlEraky, D.M., Farhat, M., Almandil, N.B., AbdulAzeez, S., & Borgio, J.F. (2023). Genomic insights into virulence factors and multi-drug resistance in Clostridium perfringens IRMC2505A. Toxins, 15(6), 359. https://doi.org/10.3390/toxins15060359
  2. Bergman, A.M. (1909). Die rote beulenkrankheit des aals. Bericht aus der Koniglichen Bayerischen Versuchsstation, 2, 10-54
  3. Bondad-Reantaso, M.G., MacKinnon, B., Karunasagar, I., Fridman, S., Alday-Sanz, V., Brun, E., Le Groumellec, M., Li, A., Surachetpong, W., Karunasagar, I., Hao, B., Dall'Occo, A., Urbani, R., & Caputo, A. (2023). Review of alternatives to antibiotic use in aquaculture. Reviews in Aquaculture, 15(4), 1421-1451. https://doi.org/10.1111/raq.12786
  4. Busschaert, P., Frans, I., Crauwels, S., Zhu, B., Willems, K., Bossier, P., Michiels, C., Verstrepen, K., Lievens, B., & Rediers, H. (2015). Comparative genome sequencing to assess the genetic diversity and virulence attributes of 15 Vibrio anguillarum isolates. Journal of Fish Diseases, 38(9), 795-807. https://doi.org/10.1111/jfd.12290
  5. Castillo, D., Alvise, P.D., Xu, R., Zhang, F., Middelboe, M., & Gram, L. (2017). Comparative genome analyses of Vibrio anguillarum strains reveal a link with pathogenicity traits. Msystems, 2(1), 10 1128. https://doi.org/10.1128/msystems.00174-16
  6. Crisafi, F., Denaro, R., Genovese, M., Yakimov, M., & Genovese, L. (2014). Application of relative real‐time PCR to detect differential expression of virulence genes in Vibrio anguillarum under standard and stressed growth conditions. Journal of Fish Diseases, 37(7), 629 640. https://doi.org/10.1111/jfd.12158
  7. Eren Eroglu, A.E., Toklu, K., & Yasa, İ. (2025a). Functional genomics of a food-related thermotolerant Acetobacter oryzifermentans strain AAB5: genetic determinants of stress response, CAZyme repertoire, and CRISPR-Cas system. Functional & Integrative Genomics, 25(1), 253. https://doi.org/10.1007/s10142-025-01765-0
  8. Eren Eroğlu, A.E., Toklu, K., Sarıkahya, N., & Yaşa, İ. (2025b). In Vitro Ferrophilic Responses of Photobacterium damselae subsp. piscicida EKL1 and characterization of the Fe (III)-Piscibactin complex. Microorganisms, 13(4), 858. https://doi.org/10.3390/microorganisms13040858

Ayrıntılar

Birincil Dil

İngilizce

Konular

Balıkçılık Yönetimi, Sucul Kültür ve Balıkçılık (Diğer)

Bölüm

Araştırma Makalesi

Yayımlanma Tarihi

14 Haziran 2026

Gönderilme Tarihi

24 Nisan 2026

Kabul Tarihi

12 Mayıs 2026

Yayımlandığı Sayı

Yıl 2026 Cilt: 43 Sayı: 3

Kaynak Göster

APA
Toklu, K., & Yaşa, İ. (2026). Identification, characterization and draft genome analysis of Vibrio anguillarum VK1 isolated from hatchery water. Ege Journal of Fisheries and Aquatic Sciences, 43(3), 216-225. https://doi.org/10.12714/egejfas.43.3.04
AMA
1.Toklu K, Yaşa İ. Identification, characterization and draft genome analysis of Vibrio anguillarum VK1 isolated from hatchery water. EgeJFAS. 2026;43(3):216-225. doi:10.12714/egejfas.43.3.04
Chicago
Toklu, Kadriye, ve İhsan Yaşa. 2026. “Identification, characterization and draft genome analysis of Vibrio anguillarum VK1 isolated from hatchery water”. Ege Journal of Fisheries and Aquatic Sciences 43 (3): 216-25. https://doi.org/10.12714/egejfas.43.3.04.
EndNote
Toklu K, Yaşa İ (01 Haziran 2026) Identification, characterization and draft genome analysis of Vibrio anguillarum VK1 isolated from hatchery water. Ege Journal of Fisheries and Aquatic Sciences 43 3 216–225.
IEEE
[1]K. Toklu ve İ. Yaşa, “Identification, characterization and draft genome analysis of Vibrio anguillarum VK1 isolated from hatchery water”, EgeJFAS, c. 43, sy 3, ss. 216–225, Haz. 2026, doi: 10.12714/egejfas.43.3.04.
ISNAD
Toklu, Kadriye - Yaşa, İhsan. “Identification, characterization and draft genome analysis of Vibrio anguillarum VK1 isolated from hatchery water”. Ege Journal of Fisheries and Aquatic Sciences 43/3 (01 Haziran 2026): 216-225. https://doi.org/10.12714/egejfas.43.3.04.
JAMA
1.Toklu K, Yaşa İ. Identification, characterization and draft genome analysis of Vibrio anguillarum VK1 isolated from hatchery water. EgeJFAS. 2026;43:216–225.
MLA
Toklu, Kadriye, ve İhsan Yaşa. “Identification, characterization and draft genome analysis of Vibrio anguillarum VK1 isolated from hatchery water”. Ege Journal of Fisheries and Aquatic Sciences, c. 43, sy 3, Haziran 2026, ss. 216-25, doi:10.12714/egejfas.43.3.04.
Vancouver
1.Kadriye Toklu, İhsan Yaşa. Identification, characterization and draft genome analysis of Vibrio anguillarum VK1 isolated from hatchery water. EgeJFAS. 01 Haziran 2026;43(3):216-25. doi:10.12714/egejfas.43.3.04