Quantitative assessment of gula pigmentation in Chironomus riparius (Diptera: Chironomidae)

Gürçay Kıvanç Akyıldız

doi:10.12714/egejfas.43.1.05

EN TR

Quantitative assessment of gula pigmentation in Chironomus riparius (Diptera: Chironomidae)

Öz

Chironomids (Diptera: Chironomidae) are among the most abundant and ecologically significant invertebrates in freshwater ecosystems. Within this group, the genus Chironomus poses considerable taxonomic challenges due to its high number of cryptic species, especially at the larval stage. Larval identification traditionally relies on qualitative morphological characters, such as the pigmentation of the gula. However, the subjective nature of these observations limits reproducibility and diagnostic accuracy. This study provides a quantitative assessment of gula pigmentation variation among Chironomus riparius larvae derived from a single egg mass. Image processing techniques were applied to convert color traits into measurable parameters, enabling objective analysis of pigmentation intensity, distribution, and optical properties. Digital quantification revealed significant intraspecific variation in gula pigmentation, even among genetically identical larvae reared under uniform laboratory conditions. These results underscore the potential of digital image analysis to improve the precision, objectivity, and reproducibility of morphological identification in chironomids. This methodological framework contributes to the standardization of qualitative traits for broader use in ecological and systematic research.

Anahtar Kelimeler

Chironomus riparius (Diptera: Chironomidae)’ta gula pigmentasyonunun nicel değerlendirilmesi

Öz

Chironomidler (Diptera: Chironomidae), tatlı su ekosistemlerinde en bol bulunan ve ekolojik açıdan en önemli omurgasız gruplarından biridir. Bu grup içerisinde yer alan Chironomus cinsi, özellikle larva evresinde çok sayıda kriptik tür içermesi nedeniyle önemli taksonomik güçlükler barındırmaktadır. Larvaların tanımlanması geleneksel olarak gula pigmentasyonu gibi nitel morfolojik karakterlere dayanmaktadır. Ancak bu tür gözlemlerin öznel yapısı, tekrarlanabilirliği ve tanısal doğruluğu sınırlandırmaktadır. Bu çalışmada, tek bir yumurta paketinden elde edilen Chironomus riparius larvalarında gula pigmentasyonundaki varyasyon nicel olarak değerlendirilmiştir. Renk özelliklerini ölçülebilir parametrelere dönüştürmek amacıyla görüntü işleme teknikleri uygulanmış; böylece pigmentasyon yoğunluğu, dağılımı ve optik özelliklerinin nesnel biçimde analiz edilmesi sağlanmıştır. Sayısal analizler, genetik olarak özdeş ve kontrollü laboratuvar koşullarında yetiştirilen larvalar arasında dahi gula pigmentasyonunda anlamlı tür içi varyasyon bulunduğunu ortaya koymuştur. Elde edilen bulgular, dijital görüntü analizinin chironomidlerde morfolojik tanımlamanın doğruluğunu, nesnelliğini ve tekrarlanabilirliğini artırma potansiyelini vurgulamaktadır. Sunulan bu metodolojik yaklaşım, nitel karakterlerin standartlaştırılmasına katkı sağlayarak ekolojik ve sistematik çalışmalarda daha geniş kullanım olanağı sunmaktadır.

Anahtar Kelimeler

Destekleyen Kurum

Bu araştırma, kamu, ticari veya kar amacı gütmeyen herhangi bir finansman kuruluşundan belirli bir hibe, fon veya başka bir destek almamıştır.

Proje Numarası

No Project Number

Etik Beyan

Bu çalışma için özel bir etik onay alınması gerekli değildir.

Teşekkür

Bu araştırma, kamu, ticari veya kar amacı gütmeyen herhangi bir finansman kuruluşundan belirli bir hibe, fon veya başka bir destek almamıştır.

Kaynakça

Abashy, M.M. (2005). Culture of chironomid larvae (Insecta- Diptera Chironomidae) under different feeding systems. Egyptian Journal of Aquatic Research, 31, 403–418.
Belleghem, S.M.V., Papa, R., Ortiz-Zuazaga, H., Hendrickx, F., Jiggins, C.D., McMillan, W.O., & Counterman, B.A. (2018). Patternize: An R package for quantifying colour pattern variation. Methods in Ecology and Evolution, 9, 390–398. https://doi.org/10.1111/2041-210X.12853
Branch, H.E. (1931). Identification of chironomid egg masses ii. Kansas Academy of Science, 34, 151–157. https://doi.org/10.2307/3624485
Brooks, S.J., Langdon, P.G., & Heiri, O. (2007). The identification and use of Palearctic Chironomidae larvae in palaeoecology. QRA Technical Guide No. 10, Quaternary Research Association, London.
Debat, V., & David, P. (2001). Mapping phenotypes: Canalization, plasticity and developmental stability. Trends in Ecology & Evolution, 16(10), 555–561. https://doi.org/10.1016/S0169-5347(01)02266-2
Ergović, V., Čerba, D., Vučković, N., & Mihaljević, Z. (2024). Chironomid pupal exuviae technique in ecological research of man-made water bodies. Water, 16(20), 2917. https://doi.org/10.3390/w16202917
Frouz J., Ali, A., & Lobinske, R.J. (2002). Influence of temperature on developmental rate, wing length, and larval head capsule size of pestiferous midge Chironomus crassicaudatus (Diptera: Chironomidae). Journal of Economic Entomology, 95(4), 699 705. https://doi.org/10.1603/0022-0493-95.4.699
GIMP. (2019). Gimp: gnu image manipulation program. The GIMP Development Team. https://www.gimp.org

Guillermo-Ferreira, R., Therézio, E.M., Gehlen, M.H., Bispo, P.C., & Marletta, A. (2014). The role of wing pigmentation, uv and fluorescence as signals in a neotropical damselfly. Journal of Insect Behavior, 27(1), 67–80. https://doi.org/10.1007/s10905-013-9406-4
Høye, T.T., Ärje, J., Bjerge, K., Hansen, O.L.P., Iosifidis, A., Leese, F., Mann, H., Meissner, K., Melvad, C., & Raitoharju, J. (2021). Deep learning and computer vision will transform entomology. Proceedings of the National Academy of Sciences, 118(2), e2002545117. https://doi.org/10.1073/pnas.2002545117
Johannsen, O.A. (1937). Part iv Chironomidae: Subfamily Chironominae. In O.A. Johannsen (Ed.), Aquatic Diptera - Eggs, Larvae, and Pupae of Aquatic Flies (pp. 1–52). Memoirs of the Cornell University Agricultural Experiment Station.
Karima, Z. (2021). Chironomidae: biology, ecology and systematics. In F.K. Perveen (Ed.), The Wonders of Diptera - Characteristics, Diversity, and Significance for the World's Ecosystems. IntechOpen. https://doi.org/10.5772/intechopen.95577
Kerr, P.H., Fisher, E.M., & Buffington, M.L. (2008). Dome lightning for insect imaging under a microscope. American Entomologist, 54(4), 198–200. https://doi.org/10.1093/ae/54.4.198
Kiknadze, I.I., Shilova, A.I., Kerkis, I.E., Shobanov, N.A., Zelentsov, N.I., Grebenyuk, I.P., Istomina, A.G., & Prasalov, V.A. (1991). Karyotypes and Larval Morphology of the Tribe Chironomini: Atlas. Novosibirsk. (in Russian)
Klingenberg, C.P. (2003). Developmental instability as a research tool: Using patterns of fluctuating asymmetry to infer the developmental origins of morphological integration. In M. Polák (Ed.), Developmental Instability: Causes and Consequences (pp. 427–442). New York. https://doi.org/10.1093/oso/9780195143454.003.0024
Klingenberg, C.P. (2019). Phenotypic plasticity, developmental instability, and robustness: The concepts and how they are connected. Frontiers in Ecology and Evolution, 7, 56. https://doi.org/10.3389/fevo.2019.00056
Kownacki, A., Szarek-Gwiazda, E., Dykas, M., Jablonska-Barna, I., & Michailova, P. (2018). Comparison of selected parts of larval morphology in some species of the genus Chironomus (Diptera: Chironomidae) using scanning electron microscope. Journal of Limnology, 77(1), 25–30. https://doi.org/10.4081/jlimnol.2018.1739
Krell, F.T. (2004). Parataxonomy vs. taxonomy in biodiversity studies - pitfalls and applicability of 'morphospecies' sorting. Biodiversity and Conservation, 13, 795 812. https://doi.org/10.1023/B:BIOC.0000011727.53780.63
Langton, P.H. (1991). A key to pupal exuviae of West Palaearctic Chironomidae. Privately published by the author.
Lehnert, M.S., Balaban, M.O., & Emmel, T.C. (2011). A new method for quantifying color of insects. Florida Entomologist, 94(2), 201–207. https://doi.org/10.1653/024.094.0212
Leszczyńska, J., Grzybkowska, M., Głowacki, L., & Dukowska, M. (2019). Environmental variables influencing chironomid assemblages (Diptera: Chironomidae) in lowland rivers of central Poland. Environmental Entomology, 48(4), 988–997. https://doi.org/10.1093/ee/nvz057
Nicacio, G., & Juen, L. (2015). Chironomids as indicators in freshwater ecosystems: An assessment of the literature. Insect Conservation and Diversity, 8(5), 393-403. https://doi.org/10.1111/icad.12123
Ninomiya, Y., Tanaka, K., & Hayakawa, Y. (2006). Mechanisms of black and white stripe pattern formation in the cuticles of insect larvae. Journal of Insect Physiology, 52, 638 645. https://doi.org/10.1016/j.jinsphys.2006.03.002
Palmer, A.R., & Strobeck, C. (1986). Fluctuating asymmetry: Measurement, analysis, patterns. Annual Review of Ecology and Systematics, 17, 391–421. https://doi.org/10.1146/annurev.es.17.110186.002135
Panis, L.I., Goddeeris, B., & Verheyen, R. (1994). On the relationship between vertical microdistribution and adaptations to oxygen stress in littoral Chironomidae. Hydrobiologia, 318, 61 67. https://doi.org/10.1007/BF00014132
Pinder, L.C.V. (1986). Biology of freshwater Chironomidae. Annual Review of Entomology, 31, 1 23. https://doi.org/10.1146/annurev.en.31.010186.000245
R Core Team (2021, September 17). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org
Schneider, C.A., Rasband, W.S., & Eliceiri, K.W. (2012). NIH image to imagej: 25 years of image analysis. Nature Methods, 9(7), 671–675. https://doi.org/10.1038/nmeth.2089
Shobanov, N.A., Shilova, A.I., &Belyanina, S.I. (1996). Extent and content of the genus Chironomus Meig. (Diptera, Chironomidae): review of world fauna (pp. 44–96). In N.A. Shobanov & T.D. Zinchenko, (Eds.), Ecology, Evolution and Systematics of Chironomids, Institute of Biology of Inland Waters and Institute of Ecology of the Volga Basin. Russian Academy of Sciences, Tolyatti, Boruk, Russia.
Stavenga, D.G., Leertouwer, H.L., & Wilts, B.D. (2014). Coloration principles of nymphaline butterflies: Thin films, melanin, ommochromes and wing scale stacking. Journal of Experimental Biology, 217(12), 2171–2180. https://doi.org/10.1242/jeb.098673
Vallenduuk, H.J., & Moller Pillot, H.K.M. (1997). Key to the larvae of Chironomus in Western Europe (pp. 1–13 + appendix). In RIZA Rapport 97.053.
Vallenduuk, H.J., & Langton, P.H. (2010). Description of imago, pupal exuviae and larva of Chironomus uliginosus and a provisional key to the larvae of the Chironomus luridus agg. (Diptera: Chironomidae). Lauterbornia, 70, 73-89.
Ward, J., & Stanford, J. (2003). Thermal responses in the evolutionary ecology of aquatic insects. Annual Review of Entomology, 27, 97–117. https://doi.org/10.1146/annurev.en.27.010182.000525
Webb, C.J., & Scholl, A. (1985). Identification of larvae of European species of Chironomus Meigen (Diptera: Chironomidae) by morphological characters. Systematic Entomology, 10, 353 372. https://doi.org/10.1111/j.1365-3113.1985.tb00143.x
Wen, C., & Guyer, D. (2012). Image-based orchard insect automated identification and classification method. Computers and Electronics in Agriculture, 89, 110-115. https://doi.org/10.1016/j.compag.2012.08.008
Wilson, R.S., & Ruse, L.P. (2005). A guide to the identification of genera of chironomid pupal exuviae occuring in Britain and Ireland (including common genera from Northern Europe) and their use in monitoring lotic and lentic fresh waters. Freshwater Biological Association Special Publication, 13, 176 p.
Wittkopp P.J., & Beldade P. (2009). Development and evolution of insect pigmentation: genetic mechanisms and the potential consequences of pleiotropy. Seminars in Cell and Developmental Biology. 20(1), 65–71. https://doi.org/10.1016/j.semcdb.2008.10.002

Ayrıntılar

Birincil Dil

İngilizce

Konular

Hidrobiyoloji, Tatlı Su Ekolojisi, Sucul Kültür ve Balıkçılık (Diğer)

Bölüm

Araştırma Makalesi

Yazarlar

Gürçay Kıvanç Akyıldız ^*
0000-0001-9610-8443
Türkiye

Yayımlanma Tarihi

18 Şubat 2026

Gönderilme Tarihi

27 Ekim 2025

Kabul Tarihi

29 Aralık 2025

Yayımlandığı Sayı

Yıl 2026 Cilt: 43 Sayı: 1

DOI

https://doi.org/10.12714/egejfas.43.1.05

IZ

https://izlik.org/JA86RC79UC

Kaynak Göster

RIS / Bibtex

APA

Akyıldız, G. K. (2026). Quantitative assessment of gula pigmentation in Chironomus riparius (Diptera: Chironomidae). Ege Journal of Fisheries and Aquatic Sciences, 43(1), 40-47. https://doi.org/10.12714/egejfas.43.1.05

AMA

1.Akyıldız GK. Quantitative assessment of gula pigmentation in Chironomus riparius (Diptera: Chironomidae). EgeJFAS. 2026;43(1):40-47. doi:10.12714/egejfas.43.1.05

Chicago

Akyıldız, Gürçay Kıvanç. 2026. “Quantitative assessment of gula pigmentation in Chironomus riparius (Diptera: Chironomidae)”. Ege Journal of Fisheries and Aquatic Sciences 43 (1): 40-47. https://doi.org/10.12714/egejfas.43.1.05.

EndNote

Akyıldız GK (01 Şubat 2026) Quantitative assessment of gula pigmentation in Chironomus riparius (Diptera: Chironomidae). Ege Journal of Fisheries and Aquatic Sciences 43 1 40–47.

IEEE

[1]G. K. Akyıldız, “Quantitative assessment of gula pigmentation in Chironomus riparius (Diptera: Chironomidae)”, EgeJFAS, c. 43, sy 1, ss. 40–47, Şub. 2026, doi: 10.12714/egejfas.43.1.05.

ISNAD

Akyıldız, Gürçay Kıvanç. “Quantitative assessment of gula pigmentation in Chironomus riparius (Diptera: Chironomidae)”. Ege Journal of Fisheries and Aquatic Sciences 43/1 (01 Şubat 2026): 40-47. https://doi.org/10.12714/egejfas.43.1.05.

JAMA

1.Akyıldız GK. Quantitative assessment of gula pigmentation in Chironomus riparius (Diptera: Chironomidae). EgeJFAS. 2026;43:40–47.

MLA

Akyıldız, Gürçay Kıvanç. “Quantitative assessment of gula pigmentation in Chironomus riparius (Diptera: Chironomidae)”. Ege Journal of Fisheries and Aquatic Sciences, c. 43, sy 1, Şubat 2026, ss. 40-47, doi:10.12714/egejfas.43.1.05.

Vancouver

1.Gürçay Kıvanç Akyıldız. Quantitative assessment of gula pigmentation in Chironomus riparius (Diptera: Chironomidae). EgeJFAS. 01 Şubat 2026;43(1):40-7. doi:10.12714/egejfas.43.1.05