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Characterization of Planococcus dechangensis isolated from a water sample of Çamaltı Saltern

Yıl 2021, Cilt: 38 Sayı: 4, 527 - 531, 15.12.2021

Pınar Çağlayan

https://doi.org/10.12714/egejfas.38.4.15

Öz


In the present study, strain MHDS3 was isolated from a water sample of Çamaltı Saltern and identified using conventional and molecular methods. 16S rRNA gene sequence analyses showed that the strain MHDS3 belonged to Planococcus dechangensis species. It gave a positive result in the Gram staining test. The cells were coccus, non-motile, aerobic, catalase positive, oxidase negative and the colony pigmentation was yellow-orange. It showed negative results for citrate utilization, indole production from tryptophane, Voges-Proskauer and methyl red. This isolate was able to grow at 10-45°C (optimally 35°C), pH 6-8 (optimally pH 7) and 3-20% NaCl (optimally 10% NaCl). It was not able to grow at 4°C, 10°C, 50°C, salt-free, 0.5%, 25%, %30 total salt, pH 4-5, and pH 9-12. Glucose, ribose, fructose, sucrose, maltose were used by the test isolate as carbon sources. Different amino acids found in the structure of animal hide such as L-lysine, L-arginine, L-cysteine, L-alanine, L-tyrosine, L-histidine were also utilized by the bacterium. During the salt production process, this bacterium may contaminate the salt which is used in the food and leather industries. The activities of harmful moderately halophilic bacteria should be prevented by effective antimicrobial applications.

Anahtar Kelimeler

Moderately halophilic bacterium Çamaltı Saltern salt production Planococcus dechangensis

Kaynakça

  • Alas, Akpolat, C., Ventosa, A., Birbir, M., Sanchez-Porro, C. & Caglayan, P. (2015). Molecular identification of moderately halophilic bacteria and extremely halophilic archaea isolated from salted sheep skins containing red and yellow discolourations. Journal of American Leather Chemists Association, 110, 211-220.
  • Caglayan, P., Birbir, M., Sánchez-Porro, C. & Ventosa, A. (2017). Screening of industrially important enzymes produced by moderately halophilic bacteria isolated from salted sheep skins of diverse origin. Journal of American Leather Chemists Association, 112, 207-216.
  • Caglayan, P., Birbir, M., Sánchez-Porro, C. & Ventosa, A. (2018). Detection of industrially potential enzymes of moderately halophilic bacteria on salted goat skins. Turkish Journal of Biochemistry, 43, 312-322. DOI: 10.1515/tjb-2017-0127
  • De la Haba, Yilmaz, P., Sánchez-Porro, C., Birbir, M. & Ventosa, A. (2011). Salimicrobium salexigens sp. nov., a moderately halophilic bacterium from salted hides. Systematic and Applied Microbiology, 34, 435-439. DOI: 10.1016/j.syapm.2011.04.002
  • Galinski, E.A. & Louis, P. (1998). Compatible solutes: ectoine production and gene expression. In A. Oren (Ed.), Microbiology and Bio-geochemistry of Hypersaline Environments (pp 40-45). Boca Raton: CRC Press.
  • Gaur, V.K., Tripathi, V., Gupta, P., Dhiman, N., Regar, R.K., Gautam, K., Srivastava, J.K., Patnaik, S., Patel, D.K., Manickam, N. (2020). Rhamnolipids from Planococcus spp. and their mechanism of action against pathogenic bacteria. Bioresource Technology, 307. DOI: 10.1016/j.biortech.2020.123206
  • Gupta, S., Sharma, P., Dev, K. & Sourirajan, A. (2016). Halophilic bacteria of Lunsu produce an array of industrially important enzymes with salt-tolerant activity. Biochemistry Research International, 1-10. DOI: 10.1155/2016/9237418
  • Guven, K., Demirci, A., Mutlu, M.B. & Korcan, S.E. (2010). Phenotypic Characterization of Halophilic Bacteria Isolated from Camaltı Saltern in Turkey. Electronic Journal of BioTechnology, 1, 11-21.
  • Engelhardt, M.A., Daly, K., Swannell, R.P.J. & Head, I.M. (2001). Isolation and characterization of a novel hydrocarbondegrading, gram-positive bacterium, isolated from interdital beach sediment, and, description of Planococcus alkanoclasticus sp. nov. Journal of Applied Microbiology, 90, 237–247. DOI: 10.1046/j.1365-2672.2001.01241
  • Erdogmus, S.F., Mutlu, B., Korcan, S.E., Guven, K. & Konuk, M. (2013). Aromatic hydrocarbon degradation by halophilic archaea isolated from Camalti Saltern, Turkey. Water Air and Soil Pollution, 224, 1449. DOI: 10.1007/s11270-013-1449-9
  • Hao, V.M. & Komagata, K. (1985). A new species of Planococcus, P. kocurii isolated from fish, frozen foods, and fish curing brine. The Journal of General and Applied Microbiology, 31, 441-455. DOI: 10.2323/jgam.31.441
  • Imhoff, J.F. (1986). Osmoregulation and compatible solutes in eubacteria. FEMS Microbiology Reviews, 39, 57-66. DOI: 10.1111/j.1574-6968.1986.tb01843.x
  • Johnson, T.R. & Case, C.L. (2010). Laboratory Experiments in Microbiology. United Kingdom: Pearson.
  • Junge, K., Gosink, J.J., Hoppe, H.G. & Staley, J.T. (1998). Arthrobacter, Brachybacterium and Planococcus isolates identified from Antarctic Sea Ice brine. Description of Planococcus mcmeekinii, sp.nov. Systematic and Applied Microbiology, 21, 306-314. DOI: 10.1016/S0723-2020(98)80038-6
  • Kami, K.F., Ghanea, M. & Babaeekhoua, L. (2020). Hydrolase-producing moderately halophilic bacteria from Eshtehard Desert (Iran). Microbiology, 89(6), 769-777. DOI: 10.1134/S0026261720060041
  • Kırtel, O., Aydın, H. & Toksoy Oner, E. (2021). Fructanogenic traits in halotolerant Bacillus licheniformis OK12 and their predicted functional significance. Journal of Applied Microbiology, 1-14. DOI: 10.1111/jam.15015
  • Kim, O.S., Cho, Y.J., Lee, K., Yoon, S.H., Kim, M., Na, H., Park, S.C., Jeon, Y.S., Lee, J. H., Yi, H., Won, S. & Chun, J. (2012). Introducing EzTaxon: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. International Journal of Systematic and Evolutionary Microbiology, 62, 716-721. DOI: 10.1099/ijs.0.038075-0
  • Mellado, E., Nieto, J.J. & Ventosa, A. (1995). Phylogenetic interferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui, Volcaniella eurihalina and Deleya halophila and reclassification of V. eurihalina as Halomonas eurihalina comb. nov. International Journal of Systematic Bacteriology, 45, 712-716. DOI: 10.1099/00207713-45-4-712
  • Mutlu, M. B. & Güven, K. (2009). Isolation and Characterization of Halophilic Bacteria from Çamaltı Saltern Turkey. New Biotechnology, 25. DOI: 10.1016/j.nbt.2009.06.340
  • Mutlu, M.B. & Güven, K. (2011). Detection of prokaryotic microbial communities of Çamaltı Saltern-Turkey by Fluorescein In Situ Hybridization (FISH) and Real Time PCR. Turkish Journal of Biology, 35, 687-695. DOI: 10.3906/biy-1010-151
  • Mutlu, M.B. & Güven, K. (2015). Bacterial diversity in Çamaltı Saltern, Turkey. Polish Journal of Microbiology, 64, 37-45.
  • Poli, A., Nicolaus, B., Denizci, A.A., Yavuzturk, B. & Kazan, D. (2012). Halomonas smyrnensis sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium from Camalti Saltern Area, Turkey. International Journal of Systematic and Evolutionary Microbiology, 63, 10-18. DOI: 10.1099/ijs.0.037036-0
  • Rohban, R., Amoozegar, M.A. & Ventosa, A. (2009). Screening and isolation of halophilic bacteria producing extracellular hydrolyses from Howz Soltan Lake, Iran. Journal of Industrial Microbiology and Biotechnology, 36, 333-340. DOI: 10.1007/s10295-008-0500-0
  • Romano, R., Giordano, A., Lama, L., Nicolaus, B. & Gambacorta, A. (2003). Planococcus rifietensis sp. nov, Isolated from Algal Mat Collected from a Sulfurous Spring in Campania (Italy). Systematic and Applied Microbiology, 26, 357-366. DOI: 10.1078/072320203322497383
  • Sánchez-Porro, C., Martín, S., Mellado, E. & Ventosa, A. (2003). Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. Journal of Applied Microbiology, 94, 295-300. DOI: 10.1046/j.1365-2672.2003.01834.x
  • Shivanand, P. & Mugeraya, G. (2011). Halophilic bacteria and their compatible solutes osmoregulation and potential applications. Current Science, 100(10), 1516-1521.
  • Szpak, P. (2011). Fish bone chemistry and ultrastructure: implications for taphonomy and stable isotope analysis. Journal of Archaeological Science, 38, 3358-3372. DOI: 10.1016/j.jas.2011.07.022
  • Wang, K., Zhang, L., Li, J., Pan., Y., Meng, L., Xu, T., Zhang, C., Liu, H., Hong, S., Huang, H. & Jiang, J. (2015). Planococcus dechangensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soils in Dechang Township, Zhaodong City, China. Antonie van Leeuwenhoek, 107, 1075-1083. DOI: 10.1007/s10482-015-0399-1
  • Ventosa, A., Garcia, M.T., Kamekura, M., Onishi, H. & Ruiz-Berraquero, F. (1989). Bacillus halophilus sp. nov., a moderately halophilic Bacillus species. Systematic and Applied Microbiology, 12, 162-166. DOI: 10.1016/S0723-2020(89)80009-8
  • Ventosa, A., Nieto, J.J. & Oren, A. (1998). Biology of moderately halophilic aerobic bacteria. Microbiology and Molecular Biology Reviews, 62, 504-544. DOI: 10.1128/MMBR.62.2.504-544.1998
  • Ventosa, A., Oren, A. & Yanhe, M. (2011). Halophiles and hypersaline environments: current research and future trends. Berlin: Springer. DOI: 10.1007/978-3-642-20198-1
  • Yaşa, I., Kahraman, O., Tekin, E. & Kocyigit, A. (2008). Isolation and molecular identification of extreme halophilic archaea from Çamaltı Saltern. Ege Journal of Fisheries and Aquatic Sciences, 25, 117-121. DOI: 10.12714/egejfas.2008.25.2.5000156581

Çamaltı Tuzlasının su örneğinden izole edilen Planococcus dechangensis’in karakterizasyonu

Yıl 2021, Cilt: 38 Sayı: 4, 527 - 531, 15.12.2021

Pınar Çağlayan

https://doi.org/10.12714/egejfas.38.4.15

Öz


Bu çalışmada, MHDS3 suşu Çamaltı Tuzlasının su örneğinden izole edilmiştir ve geleneksel ve moleküler yöntemlerle tanımlanmıştır. 16S rRNA gen dizi analizleri bu suşun Planococcus dechangensis türü olduğunu göstermiştir. Gram pozitif, kok formunda, hareketsiz, aerobik, katalaz pozitif, oksidaz negatiftir ve sarı-turuncu koloni pigmentasyonu göstermiştir. Bu suş sitrat kullanımı, triptofanda indol üretimi, Voges-Proskauer ve metil kırmızısı testlerinde negatif sonuç vermiştir. Bu izolat 10-45°C’de (optimum 35°C), pH 6-8’de (optimum pH 7) ve %3-20 NaCl konsantrasyonunda (optimum %10 NaCl) gelişebilmektedir. 4°C, 10°C, 50°C’de, tuzsuz ortamda, %0.5, %25, %30 toplam tuz oranında, pH 4-5 ve pH 9-12’de gelişme göstermemiştir. Glukoz, riboz, fruktoz, sukroz, maltoz bu izolat tarafından karbon kaynağı olarak kullanılmıştır. Hayvan derisinin yapısında bulunan L-lizin, L-arjinin, L-sistein, L-alanin, L-tirozin, L-histidin gibi farklı amino asitler bu izolat tarafından da kullanılmıştır. Tuz üretimi aşamasında, bu bakteri gıda ve deri endüstrisinde kullanılmak üzere üretilen tuzu kontamine edebilir. Ilımlı halofil bakterilerin zararlı etkileri uygun antimikrobiyal uygulamalar ile engellenmelidir.

Anahtar Kelimeler

Ilımlı halofil bakteri Çamaltı Tuzlası tuz üretimi Planococcus dechangensis

Kaynakça

  • Alas, Akpolat, C., Ventosa, A., Birbir, M., Sanchez-Porro, C. & Caglayan, P. (2015). Molecular identification of moderately halophilic bacteria and extremely halophilic archaea isolated from salted sheep skins containing red and yellow discolourations. Journal of American Leather Chemists Association, 110, 211-220.
  • Caglayan, P., Birbir, M., Sánchez-Porro, C. & Ventosa, A. (2017). Screening of industrially important enzymes produced by moderately halophilic bacteria isolated from salted sheep skins of diverse origin. Journal of American Leather Chemists Association, 112, 207-216.
  • Caglayan, P., Birbir, M., Sánchez-Porro, C. & Ventosa, A. (2018). Detection of industrially potential enzymes of moderately halophilic bacteria on salted goat skins. Turkish Journal of Biochemistry, 43, 312-322. DOI: 10.1515/tjb-2017-0127
  • De la Haba, Yilmaz, P., Sánchez-Porro, C., Birbir, M. & Ventosa, A. (2011). Salimicrobium salexigens sp. nov., a moderately halophilic bacterium from salted hides. Systematic and Applied Microbiology, 34, 435-439. DOI: 10.1016/j.syapm.2011.04.002
  • Galinski, E.A. & Louis, P. (1998). Compatible solutes: ectoine production and gene expression. In A. Oren (Ed.), Microbiology and Bio-geochemistry of Hypersaline Environments (pp 40-45). Boca Raton: CRC Press.
  • Gaur, V.K., Tripathi, V., Gupta, P., Dhiman, N., Regar, R.K., Gautam, K., Srivastava, J.K., Patnaik, S., Patel, D.K., Manickam, N. (2020). Rhamnolipids from Planococcus spp. and their mechanism of action against pathogenic bacteria. Bioresource Technology, 307. DOI: 10.1016/j.biortech.2020.123206
  • Gupta, S., Sharma, P., Dev, K. & Sourirajan, A. (2016). Halophilic bacteria of Lunsu produce an array of industrially important enzymes with salt-tolerant activity. Biochemistry Research International, 1-10. DOI: 10.1155/2016/9237418
  • Guven, K., Demirci, A., Mutlu, M.B. & Korcan, S.E. (2010). Phenotypic Characterization of Halophilic Bacteria Isolated from Camaltı Saltern in Turkey. Electronic Journal of BioTechnology, 1, 11-21.
  • Engelhardt, M.A., Daly, K., Swannell, R.P.J. & Head, I.M. (2001). Isolation and characterization of a novel hydrocarbondegrading, gram-positive bacterium, isolated from interdital beach sediment, and, description of Planococcus alkanoclasticus sp. nov. Journal of Applied Microbiology, 90, 237–247. DOI: 10.1046/j.1365-2672.2001.01241
  • Erdogmus, S.F., Mutlu, B., Korcan, S.E., Guven, K. & Konuk, M. (2013). Aromatic hydrocarbon degradation by halophilic archaea isolated from Camalti Saltern, Turkey. Water Air and Soil Pollution, 224, 1449. DOI: 10.1007/s11270-013-1449-9
  • Hao, V.M. & Komagata, K. (1985). A new species of Planococcus, P. kocurii isolated from fish, frozen foods, and fish curing brine. The Journal of General and Applied Microbiology, 31, 441-455. DOI: 10.2323/jgam.31.441
  • Imhoff, J.F. (1986). Osmoregulation and compatible solutes in eubacteria. FEMS Microbiology Reviews, 39, 57-66. DOI: 10.1111/j.1574-6968.1986.tb01843.x
  • Johnson, T.R. & Case, C.L. (2010). Laboratory Experiments in Microbiology. United Kingdom: Pearson.
  • Junge, K., Gosink, J.J., Hoppe, H.G. & Staley, J.T. (1998). Arthrobacter, Brachybacterium and Planococcus isolates identified from Antarctic Sea Ice brine. Description of Planococcus mcmeekinii, sp.nov. Systematic and Applied Microbiology, 21, 306-314. DOI: 10.1016/S0723-2020(98)80038-6
  • Kami, K.F., Ghanea, M. & Babaeekhoua, L. (2020). Hydrolase-producing moderately halophilic bacteria from Eshtehard Desert (Iran). Microbiology, 89(6), 769-777. DOI: 10.1134/S0026261720060041
  • Kırtel, O., Aydın, H. & Toksoy Oner, E. (2021). Fructanogenic traits in halotolerant Bacillus licheniformis OK12 and their predicted functional significance. Journal of Applied Microbiology, 1-14. DOI: 10.1111/jam.15015
  • Kim, O.S., Cho, Y.J., Lee, K., Yoon, S.H., Kim, M., Na, H., Park, S.C., Jeon, Y.S., Lee, J. H., Yi, H., Won, S. & Chun, J. (2012). Introducing EzTaxon: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. International Journal of Systematic and Evolutionary Microbiology, 62, 716-721. DOI: 10.1099/ijs.0.038075-0
  • Mellado, E., Nieto, J.J. & Ventosa, A. (1995). Phylogenetic interferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui, Volcaniella eurihalina and Deleya halophila and reclassification of V. eurihalina as Halomonas eurihalina comb. nov. International Journal of Systematic Bacteriology, 45, 712-716. DOI: 10.1099/00207713-45-4-712
  • Mutlu, M. B. & Güven, K. (2009). Isolation and Characterization of Halophilic Bacteria from Çamaltı Saltern Turkey. New Biotechnology, 25. DOI: 10.1016/j.nbt.2009.06.340
  • Mutlu, M.B. & Güven, K. (2011). Detection of prokaryotic microbial communities of Çamaltı Saltern-Turkey by Fluorescein In Situ Hybridization (FISH) and Real Time PCR. Turkish Journal of Biology, 35, 687-695. DOI: 10.3906/biy-1010-151
  • Mutlu, M.B. & Güven, K. (2015). Bacterial diversity in Çamaltı Saltern, Turkey. Polish Journal of Microbiology, 64, 37-45.
  • Poli, A., Nicolaus, B., Denizci, A.A., Yavuzturk, B. & Kazan, D. (2012). Halomonas smyrnensis sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium from Camalti Saltern Area, Turkey. International Journal of Systematic and Evolutionary Microbiology, 63, 10-18. DOI: 10.1099/ijs.0.037036-0
  • Rohban, R., Amoozegar, M.A. & Ventosa, A. (2009). Screening and isolation of halophilic bacteria producing extracellular hydrolyses from Howz Soltan Lake, Iran. Journal of Industrial Microbiology and Biotechnology, 36, 333-340. DOI: 10.1007/s10295-008-0500-0
  • Romano, R., Giordano, A., Lama, L., Nicolaus, B. & Gambacorta, A. (2003). Planococcus rifietensis sp. nov, Isolated from Algal Mat Collected from a Sulfurous Spring in Campania (Italy). Systematic and Applied Microbiology, 26, 357-366. DOI: 10.1078/072320203322497383
  • Sánchez-Porro, C., Martín, S., Mellado, E. & Ventosa, A. (2003). Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. Journal of Applied Microbiology, 94, 295-300. DOI: 10.1046/j.1365-2672.2003.01834.x
  • Shivanand, P. & Mugeraya, G. (2011). Halophilic bacteria and their compatible solutes osmoregulation and potential applications. Current Science, 100(10), 1516-1521.
  • Szpak, P. (2011). Fish bone chemistry and ultrastructure: implications for taphonomy and stable isotope analysis. Journal of Archaeological Science, 38, 3358-3372. DOI: 10.1016/j.jas.2011.07.022
  • Wang, K., Zhang, L., Li, J., Pan., Y., Meng, L., Xu, T., Zhang, C., Liu, H., Hong, S., Huang, H. & Jiang, J. (2015). Planococcus dechangensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soils in Dechang Township, Zhaodong City, China. Antonie van Leeuwenhoek, 107, 1075-1083. DOI: 10.1007/s10482-015-0399-1
  • Ventosa, A., Garcia, M.T., Kamekura, M., Onishi, H. & Ruiz-Berraquero, F. (1989). Bacillus halophilus sp. nov., a moderately halophilic Bacillus species. Systematic and Applied Microbiology, 12, 162-166. DOI: 10.1016/S0723-2020(89)80009-8
  • Ventosa, A., Nieto, J.J. & Oren, A. (1998). Biology of moderately halophilic aerobic bacteria. Microbiology and Molecular Biology Reviews, 62, 504-544. DOI: 10.1128/MMBR.62.2.504-544.1998
  • Ventosa, A., Oren, A. & Yanhe, M. (2011). Halophiles and hypersaline environments: current research and future trends. Berlin: Springer. DOI: 10.1007/978-3-642-20198-1
  • Yaşa, I., Kahraman, O., Tekin, E. & Kocyigit, A. (2008). Isolation and molecular identification of extreme halophilic archaea from Çamaltı Saltern. Ege Journal of Fisheries and Aquatic Sciences, 25, 117-121. DOI: 10.12714/egejfas.2008.25.2.5000156581
Toplam 32 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Yapısal Biyoloji
Bölüm Kısa Araştırmalar
Yazarlar

Pınar Çağlayan 0000-0002-0238-1754

Yayımlanma Tarihi 15 Aralık 2021
Gönderilme Tarihi 30 Nisan 2021
Yayımlandığı Sayı Yıl 2021Cilt: 38 Sayı: 4

Kaynak Göster

APA Çağlayan, P. (2021). Characterization of Planococcus dechangensis isolated from a water sample of Çamaltı Saltern. Ege Journal of Fisheries and Aquatic Sciences, 38(4), 527-531. https://doi.org/10.12714/egejfas.38.4.15