Expression of cytochrome P450 aromatase isoforms in female Alburnus tarichi (Guldenstaedtii, 1814)

Güler Ünal; Emily Marquez; Mara O'brien; Pericles Stavropoulos; Ian P. Callard

doi:10.12714/egejfas.38.2.15

TR EN

Dişi Van balığı (Alburnus tarichi, Guldenstaedtii, 1814)’nda sitokrom P450 aromataz izoformlarının ekspresyonu

Öz

Bu çalışmanın amacı, Van Gölü’nden yakalanan dişi Alburnus tarichi’de P450 aromataz izoformları olan beyin kaynaklı Cyp19b ve ovaryum kaynaklı Cyp19a genlerini klonlanmak ve üreme bakımından ovaryum gelişimi engellenmiş (RA) ve ovaryum gelişimi normal olan (RN) balıklardan alınan hypothalamus ve ovaryum örneklerinde bu genlerin ekspresyon seviyelerini belirlemektir. Cyp19b gen ekspersyonu beyinde, Cyp19a gen ekspresyonu ise ovaryumda daha fazlaydı. Cyp19b mRNA seviyesi, RA balıkların ovaryum ve hipotalamusunda, RN balıkların ovaryum ve hipotalamusundan belirgin olarak düşüktü (P<0.05). CYP19a ekspresyon seviyesi, hem RN ve hem de RA balıkların hipotalamusunda fark bulunmazken RA balıkların ovaryumunda, RN balıkların ovaryumundan belirgin olarak düşüktü (P<0.05). Bu sonuçlara bize, RA balıkların populasyonun bir parçası olduğunu ve bu balıkların endokrin bozucu bileşik veya bileşiklere diğer balıklardan daha çok duyarlı olabileceğini düşündürmektedir.

Anahtar Kelimeler

Destekleyen Kurum

This work was supported by National Institue of Environmental Health Sciences (NIEHS)

Proje Numarası

NIEHS-07381

Teşekkür

This work was supported by National Institue of Environmental Health Sciences (NIEHS) 07381 to Dr. Ian P. Callard. This work was supported by National Institue of Environmental Health Sciences (NIEHS) 07381 to Dr. Ian P. Callard. We want to thank Kellie A. Cotter and Rebecca Meyer for technical advice, and Burak Kaptaner, Ertugrul Kankaya and Ahmet R. Oğuz for fish sampling.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Güler Ünal ^*
0000-0003-1035-4809
Türkiye

Emily Marquez
0000-0003-1391-4664
United States

Mara O'brien
0000-0002-5646-2541
United States

Pericles Stavropoulos
0000-0003-0985-6203
United States

Ian P. Callard
United States

Yayımlanma Tarihi

15 Haziran 2021

Gönderilme Tarihi

14 Ocak 2021

Kabul Tarihi

22 Mart 2021

Yayımlandığı Sayı

Yıl 1970 Cilt: 38 Sayı: 2

DOI

https://doi.org/10.12714/egejfas.38.2.15

IZ

https://izlik.org/JA82FZ77NW

Kaynak Göster

RIS / Bibtex

APA

Ünal, G., Marquez, E., O’brien, M., Stavropoulos, P., & Callard, I. P. (2021). Expression of cytochrome P450 aromatase isoforms in female Alburnus tarichi (Guldenstaedtii, 1814). Ege Journal of Fisheries and Aquatic Sciences, 38(2), 247-252. https://doi.org/10.12714/egejfas.38.2.15

Dişi Van balığı (Alburnus tarichi, Guldenstaedtii, 1814)’nda sitokrom P450 aromataz izoformlarının ekspresyonu

Dişi Van balığı (Alburnus tarichi, Guldenstaedtii, 1814)’nda sitokrom P450 aromataz izoformlarının ekspresyonu

Öz

Anahtar Kelimeler