Expression of cytochrome P450 aromatase isoforms in female Alburnus tarichi (Guldenstaedtii, 1814)

Güler Ünal; Emily Marquez; Mara O'brien; Pericles Stavropoulos; Ian P. Callard

doi:10.12714/egejfas.38.2.15

TR EN

Dişi Van balığı (Alburnus tarichi, Guldenstaedtii, 1814)’nda sitokrom P450 aromataz izoformlarının ekspresyonu

Öz

Bu çalışmanın amacı, Van Gölü’nden yakalanan dişi Alburnus tarichi’de P450 aromataz izoformları olan beyin kaynaklı Cyp19b ve ovaryum kaynaklı Cyp19a genlerini klonlanmak ve üreme bakımından ovaryum gelişimi engellenmiş (RA) ve ovaryum gelişimi normal olan (RN) balıklardan alınan hypothalamus ve ovaryum örneklerinde bu genlerin ekspresyon seviyelerini belirlemektir. Cyp19b gen ekspersyonu beyinde, Cyp19a gen ekspresyonu ise ovaryumda daha fazlaydı. Cyp19b mRNA seviyesi, RA balıkların ovaryum ve hipotalamusunda, RN balıkların ovaryum ve hipotalamusundan belirgin olarak düşüktü (P<0.05). CYP19a ekspresyon seviyesi, hem RN ve hem de RA balıkların hipotalamusunda fark bulunmazken RA balıkların ovaryumunda, RN balıkların ovaryumundan belirgin olarak düşüktü (P<0.05). Bu sonuçlara bize, RA balıkların populasyonun bir parçası olduğunu ve bu balıkların endokrin bozucu bileşik veya bileşiklere diğer balıklardan daha çok duyarlı olabileceğini düşündürmektedir.

Anahtar Kelimeler

Supporting Institution

This work was supported by National Institue of Environmental Health Sciences (NIEHS)

Project Number

NIEHS-07381

Thanks

This work was supported by National Institue of Environmental Health Sciences (NIEHS) 07381 to Dr. Ian P. Callard. This work was supported by National Institue of Environmental Health Sciences (NIEHS) 07381 to Dr. Ian P. Callard. We want to thank Kellie A. Cotter and Rebecca Meyer for technical advice, and Burak Kaptaner, Ertugrul Kankaya and Ahmet R. Oğuz for fish sampling.

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Güler Ünal ^*
0000-0003-1035-4809
Türkiye

Emily Marquez
0000-0003-1391-4664
United States

Mara O'brien
0000-0002-5646-2541
United States

Pericles Stavropoulos
0000-0003-0985-6203
United States

Ian P. Callard
United States

Publication Date

June 15, 2021

Submission Date

January 14, 2021

Acceptance Date

March 22, 2021

Published in Issue

Year 1970 Volume: 38 Number: 2

DOI

https://doi.org/10.12714/egejfas.38.2.15

IZ

https://izlik.org/JA82FZ77NW

Cite

RIS / Bibtex

APA

Ünal, G., Marquez, E., O’brien, M., Stavropoulos, P., & Callard, I. P. (2021). Expression of cytochrome P450 aromatase isoforms in female Alburnus tarichi (Guldenstaedtii, 1814). Ege Journal of Fisheries and Aquatic Sciences, 38(2), 247-252. https://doi.org/10.12714/egejfas.38.2.15

Expression of cytochrome P450 aromatase isoforms in female Alburnus tarichi (Guldenstaedtii, 1814)

Dişi Van balığı (Alburnus tarichi, Guldenstaedtii, 1814)’nda sitokrom P450 aromataz izoformlarının ekspresyonu

Öz

Anahtar Kelimeler