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İzmir Çamaltı Tuzlası’ndan izole edilen endüstriyel potansiyele sahip poliekstremotolerant bakteri suşlarının (Kocuria, Virgibacillus ve Halomonas) karakterizasyonu

Year 2025, Volume: 42 Issue: 4, 307 - 316

Ebru Tekin , Mustafa Ateş

Abstract

Bu çalışmada, endüstriyel açıdan önemli halotolerant bakteri suşlarının İzmir Çamaltı Tuzlası’ndan alınan deniz suyu ve yığın örneklerinden izolasyonu, saflaştırılması ve tanılanması gerçekleştirilmiştir. Seçilen üç izolat, fenotipik, biyokimyasal ve kısmi 16S rRNA dizi analizleri ile kapsamlı şekilde incelenmiş ve türler erişim numaraları alınarak GenBank’a kayıt edilmiştir.Türe özgü filogenetik ağaçlar MEGA6.06 programı kullanılarak oluşturulmuştur. Suşların sırasıyla Firmicutes, Actinobacteria ve Gammaproteobacteria filumlarına; %95 benzerlik ile Virgibacillus, %99 benzerlik ile Kocuria ve %99 benzerlik ile Halomonas cinslerine ait olduğu belirlenmiştir. Suşlar Kocuria sp. CT-6 (KP238667), Virgibacillus sp. CT-5 (KP238666) ve Halomonas sp. CS-5 (KP238669) olarak adlandırılmıştır. Fenotipik karakterizasyona göre Virgibacillus sp. CT-5 termotolerant olup 20-55 °C aralığında büyüyebilmektedir; Kocuria sp. CT-6 psikrotolerant olup 4-37 °C aralığında yaşamaktadır; Halomonas sp. CS-5 ise hem psikrotolerant hem termotolerant olup 4-45 °C aralığında gelişim göstermektedir. Tüm suşlar alkalitolerant olup pH 5-11 aralığında büyüyebilmekte; halotolerant olup sırasıyla Virgibacillus sp. CT-5 için %0-22 (w/v), Kocuria sp. CT-6 için %0-19 (w/v) ve Halomonas sp. CS-5 için %0-25 (w/v) NaCl konsantrasyonlarında gelişim gösterebilmektedir. Bu çalışmada, İzmir Çamaltı Tuzlası’ndan izole edilen üç poliekstremotolerant bakteri suşu- Kocuria sp. CT-6, Virgibacillus sp. CT-5 ve Halomonas sp. CS-5- bildirilmiştir. Bu suşlar, birden fazla tolerans özelliğine sahip olup, endüstriyel uygulamalar için büyük bir öneme sahiptir. Bu çalışma, bu bölgeden poliekstremotolerant suşların rapor edildiği ilk çalışmadır.

Keywords

Poliekstremotolerant halotolerant alkalitolerant termotolerant Virgibacillus sp. Halomonas sp. Kocuria sp.

Project Number

2011 FEN 010

References

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Characterization of polyextremotolerant bacterial strains (Kocuria, Virgibacillus, and Halomonas) with industrial potential isolated from the İzmir Çamaltı Saltern

Year 2025, Volume: 42 Issue: 4, 307 - 316

Ebru Tekin , Mustafa Ateş

Abstract

In this study, industrially important halotolerant bacterial strains were isolated and identified from seawater and bulk samples from the İzmir Çamaltı Saltern. Three selected isolates underwent comprehensive analyses including phenotypic, biochemical, and partial 16S rRNA gene sequencing and accession numbers were taken from GenBank. Species specific trees were constructed using MEGA 6.06 software. The isolates were classified within the phyla Firmicutes, Actinobacteria, and Gammaproteobacteria, exhibiting highest sequence similarity to the genera Virgibacillus (95%), Kocuria (99%), and Halomonas (99%), respectively. The strains were designated as Virgibacillus sp. CT-5 (KP238666), Kocuria sp. CT-6 (KP238667), and Halomonas sp. CS-5 (KP238669). Phenotypic characterization revealed that Virgibacillus sp. CT-5 is thermotolerant, growing at 20–55 °C; Kocuria sp. CT-6 is psychrotolerant, growing at 4-37 °C; and Halomonas sp. CS-5 exhibits both psychrotolerance and thermotolerance, growing at 4-45 °C. All strains were alkalitolerant, thriving within a pH range of 5-11, and halotolerant, with growth observed at 0-22% (w/v) NaCl for Virgibacillus sp. CT-5, 0-19% (w/v) for Kocuria sp. CT-6, and 0-25% (w/v) for Halomonas sp. CS-5. This study presents three polyextremotolerant bacterial strains -Kocuria sp. CT-6, Virgibacillus sp. CT-5, and Halomonas sp. CS-5- isolated from the İzmir Çamaltı Saltern. Their multiple tolerance traits render them highly promising candidates for various industrial applications. This is the first report documenting the polyextremotolerance of these strains from this area.

Keywords

Polyextremotolerant halotolerant alkalitolerant thermotolerant Virgibacillus sp. Halomonas sp. Kocuria sp.

Ethical Statement

NEthical approval was not necessary for the study.

Supporting Institution

Ege University Scientific Research Council

Project Number

2011 FEN 010

Thanks

We are thankful for the support of Ege University Scientific Research Council.

References

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  • Christian, J.H., & Waltho J.A. (1962). Solute concentrations within cells of halophilic and non-halophilic bacteria. Journal of Biochimica et Biophysica Acta, 65, 506-508. https://doi.org/10.1016/0006-3002(62)90453-5
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  • Fay, G.D., & Barry, A.L. (1972). Rapid ornithine decarboxylase test for the identification of Enterobacteriaceae. Journal of Applied Microbiology, 23(4), 710-713. https://doi.org/10.1128/am.23.4.710-713.1972
  • Goldschmidt, M.C., & Lockhart, B.M. (1971). Rapid methods for determining decarboxylase activity: arginine decarboxylase. Journal of Applied Microbiology, 22(3), 350–357. https://doi.org/10.1128/am.22.3.350-357.1971
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  • Kazak Sarilmiser, H., Ates, O., Ozdemir, G., Arga, K.Y., & Toksoy Oner, E. (2015). Effective stimulating factors for microbial levan production by Halomonas smyrnensis AAD6T. Journal of Bioscience and Bioengineering, 119(4), 455 463. https://doi.org/10.1016/j.jbiosc.2014.09.019
  • Kim, K.K., Jin, L., Yang, H.C., & Lee, S.T. (2007). Halomonas gomseomensis sp. nov., Halomonas janggokensis sp. nov., Halomonas salaria sp. nov. and Halomonas denitrificans sp. nov., moderately halophilic bacteria isolated from saline water. International Journal of Systematic Evolutionary Microbiology, 57(4), 675 681. https://doi.org/10.1099/ijs.0.64767-0
  • Kim, S.B., Nedashkovskaya, O.I., Mikhailov, V.V., Han, S.K., Kim, K.O., Rhee, M.S., & Bae, K.S. (2004). Kocuria marina sp. nov., a novel actinobacterium isolated from marine sediment. International Journal of Systematic Evolutionary Microbiology, 54(Pt 5), 1617 1620. https://doi.org/10.1099/ijs.0.02742-0 Koru, E. (2004). Artemia and it’s importance in Çamaltı Saltworks (Izmir, Turkey) ecosystem. Ege University Journal of Fisheries and Aquatic Sciences, 21(1-2), 187-189.
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  • Lam, M.Q., Nik Mut, N.N., Thevarajoo, S., Chen, S.J., Selvaratnam, C., Hussin, H., Jamaluddin, H., & Chong, C.S. (2018). Characterization of detergent compatible protease from halophilic Virgibacillus sp. CD6. 3 Biotechnology, 8(2), 104. https://doi.org/10.1007/s13205-018-1133-2
  • Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J., & Higgins, D.G. (2007). Clustal W and Clustal X version 2.0. Bioinformatics, 23(21), 2947 2948. https://doi.org/10.1093/bioinformatics/btm404 Moreno, M.L., Perez, D., Garcia, M.T., & Mellado, E. (2013). Halophilic bacteria as a source of novel hydrolytic enzymes. Life, 3(1), 38-51. https://doi.org/10.3390/life3010038
  • Mutlu, M.B., & Güven, K. (2015) Bacterial diversity in Çamaltı Saltern, Turkey. Polish Journal of Microbiology, 64(1), 37–45.
  • Poli, A., Nicolaus, B., Denizci, A.A., Yavuzturk, B., & Kazan, D. (2013). Halomonas smyrnensis sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium. International Journal of Systematic Evolutionary Microbiology, 63, 10 18. https://doi.org/10.1099/ijs.0.037036-0
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There are 45 citations in total.

Details

Primary Language English
Subjects Marine and Estuarine Ecology, Fish Pests and Diseases
Journal Section Research Article
Authors

Ebru Tekin 0000-0003-2623-1977

Mustafa Ateş 0000-0002-9305-3480

Project Number 2011 FEN 010
Early Pub Date December 1, 2025
Publication Date December 3, 2025
Submission Date June 23, 2025
Acceptance Date September 17, 2025
Published in Issue Year 2025 Volume: 42 Issue: 4

Cite

APA Tekin, E., & Ateş, M. (2025). Characterization of polyextremotolerant bacterial strains (Kocuria, Virgibacillus, and Halomonas) with industrial potential isolated from the İzmir Çamaltı Saltern. Ege Journal of Fisheries and Aquatic Sciences, 42(4), 307-316.