Preliminary study on monoculture establishment and growth assessment of three Chlorophyta microalgae from Shalimar Garden, Lahore, Pakistan

Hamna Sohail; Ghazala Yasmeen Butt

doi:10.12714/egejfas.43.2.03

EN TR

Preliminary study on monoculture establishment and growth assessment of three Chlorophyta microalgae from Shalimar Garden, Lahore, Pakistan

Abstract

Freshwater microalgal samples were collected from Shalimar Garden, Lahore, Pakistan, and successfully cultured to establish pure monocultures. Three Chlorophyta species Chlorella ellipsoidea, Pediastrum obtusum, and Scenedesmus incrassatulus var. capitatus were taxonomically identified under an inverted microscope and selected for monoculture studies. Monocultures were maintained on Bold’s Basal Medium (BBM) and CHU-10 medium using the streaking technique. Peak biomass in fresh weight on BBM was recorded on the 18th day, reaching 2.12 g, 1.15 g, and 1.41 g for C. ellipsoidea, P. obtusum, and S. incrassatulus var. capitatus, respectively, with corresponding maximum growth rates of 0.004-0.009 g/day. In CHU-10, peak biomass and growth rates were notably lower (0.42-0.35 g; 0.001-0.003 g/day). By day 21, biomass and growth declined in both media, indicating nutrient depletion and onset of the decline phase. Net weight gains were substantially higher in BBM (1.38 g, 0.73 g, 0.94 g) compared to CHU-10 (0.34 g, 0.29 g, 0.26 g), and these differences were statistically significant (p<0.0001) demonstrating BBM’s superior performance in supporting faster growth and higher biomass accumulation. This study represents the first successful monoculturing of these three Chlorophyta species from the selected Shalimar Garden, Lahore, Pakistan, representing preliminary investigation thus providing baseline data that can guide future applied research in algal biotechnology and environmental studies.

Keywords

Pakistan'ın Lahor kentindeki Shalimar Bahçesinden elde edilen üç Chlorophyta mikroalg türünün monokültürü ve büyümesine ilişkin ön çalışma

Öz

Tatlı su mikroalg örnekleri Pakistan'ın Lahor kentindeki Shalimar bahçesinden toplanmış ve saf monokültürler oluşturmak için başarıyla kültüre alınmıştır. Üç Chlorophyta türü olan Chlorella ellipsoidea, Pediastrum obtusum ve Scenedesmus incrassatulus var. capitatus, ters mikroskop altında taksonomik olarak tanımlanmış ve monokültür çalışmaları için seçilmiştir. Monokültürler, Bold's Basal Medium (BBM) ve CHU-10 besiyerinde çizgi tekniği kullanılarak muhafaza edilmiştir. BBM besi yerinde taze ağırlık olarak en yüksek biyokütle, 18. günde kaydedilmiş ve C. ellipsoidea, P. obtusum ve S. incrassatulus var. capitatus türleri için sırasıyla 2,12 g, 1,15 g ve 1,41 g'a ulaşmış ve maksimum büyüme oranları ise 0,004-0,009 g/gün olarak tespit edilmiştir. CHU-10 besi yerinde, biyokütle ve büyüme oranlarının pik değerleri belirgin şekilde daha düşük (0,42-0,35 g; 0,001-0,003 g/gün) olduğu saptanmıştır. 21. günde, her iki ortamda da biyokütle ve büyüme azalmış, bu da besin maddelerinin tükendiğini ve buna bağlı düşüş aşamasının başladığını göstermektedir. Net ağırlık artışları, CHU-10 besiyerine (0,34 g, 0,29 g, 0,26 g) kıyasla BBM besiyerinde (1,38 g, 0,73 g, 0,94 g) önemli ölçüde daha yüksek olduğu ve bu farkların istatistiksel olarak anlamlı olduğu (p<0,0001) saptanmıştır. Bu da BBM'nin daha hızlı büyümeyi ve daha yüksek biyokütle birikimini destekleme konusunda üstün performansını göstermektedir. Bu çalışma, Pakistan'ın Lahor kentindeki Shalimar bahçesinden seçilen bu üç Chlorophyta türünün ilk başarılı monokültür çalışmasını temsil etmekte olup, alg biyoteknolojisi ve çevre çalışmaları alanlarında gelecekteki uygulamalı araştırmalara rehberlik edebilecek temel veriler sağlayan öncü bir araştırma niteliğindedir.

Anahtar Kelimeler

Supporting Institution

This research was self-funded and did not receive financial support from any institution.

Ethical Statement

This article does not contain any human or animal studies performed by any authors. No specific ethical approval was necessary for this study.

Thanks

The authors would like to thank Botany Department, Government College University, Lahore, for providing laboratory facilities.

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Details

Primary Language

English

Subjects

Botany (Other) , Hydrobiology , Freshwater Ecology , Aquaculture

Journal Section

Research Article

Authors

Hamna Sohail ^*
0009-0004-7048-0457
Pakistan

Ghazala Yasmeen Butt
0000-0001-7382-7613
Pakistan

Publication Date

March 31, 2026

Submission Date

December 28, 2025

Acceptance Date

March 11, 2026

Published in Issue

Year 1970 Volume: 43 Number: 2

DOI

https://doi.org/10.12714/egejfas.43.2.03

IZ

https://izlik.org/JA52EP97DY

Cite

RIS / Bibtex

APA

Sohail, H., & Butt, G. Y. (2026). Preliminary study on monoculture establishment and growth assessment of three Chlorophyta microalgae from Shalimar Garden, Lahore, Pakistan. Ege Journal of Fisheries and Aquatic Sciences, 43(2), 112-119. https://doi.org/10.12714/egejfas.43.2.03