EN
Determination of important enzymes and antimicrobial resistances of gram-positive haloalkaliphilic bacteria isolated from Salda Lake
Abstract
As an extreme environment, soda lakes harbor various haloalkaliphilic microorganisms. Salda Lake is one of the natural soda lake (pH˃9) in Turkey. Haloalkaliphiles are unique microorganisms in their ability to live in high alkaline and high saline conditions, and play an important role in biodegradation and bioremediation of hydrocarbons. Hence, the aims of this study were to isolate haloalkaliphilic bacteria from water sample of Salda Lake, to identify these isolates by both conventional and molecular methods, to screen their industrially important enzymes, and to investigate their antimicrobial resistance profiles. Six isolates were identified as Bacillus horneckiae, Bacillus subtilis, Bacillus paramycoides, Bacillus pumilus, Staphylococcus epidermidis, Bacillus haynesii according to 16S rRNA gene sequencing analysis. The industrially important enzymes (amylase, cellulase, pullulanase, lipase, urease, protease, caseinase, oxidase, catalase) were produced by haloalkaliphilic isolates. These enzymes maybe used in alkaline and saline industrial processes. Although Bacillus subtilis was susceptible to all antibiotics, other isolates showed resistance to at least one antibiotic. The resistance against antibiotics were found as ampicillin/sulbactam 83%, amoxycillin/clavulanic acid 83%, ampicillin 67%, mupirocin 67%, chloramphenicol 50%, tetracycline 50%, imipenem 50%, meropenem 50%, cefadroxil 17%. These bacteria may have develope resistance to antibiotics that entering their natural environment in different ways.
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- Ammar, Y.B., Matsubara, T., Ito, K., Lizuka, M., Limpaseni, T., Pongsawasdi, P. & Minamiura, N. (2002). Characterization of a thermostable levansucrase from Bacillus sp. TH4-2 capable of producing high molecular weight levan at high temperature. Journal of Biotechnology, 99, 111-119. DOI: 10.1016/s0168-1656(02)00160-8 Arahal, D.R., Dewhirst, F.E., Paster, B.J., Volcani, B.E. & Ventosa, A. (1996). Phylogenetic analyses of some extremely halophilic archaea isolated from dead sea water, determined on the basis of their 16S rRNA sequences. Applied and Environmental Microbiology, 62, 3779-3786. DOI: 10.1128/AEM.62.10.3779-3786.1996 Aygan, A. & Arikan, B. (2008). A new haloalkaliphilic, thermostable endoglucanase from moderately halophilic Bacillus sp. C14 isolated from Van Soda Lake. International Journal of Agriculture and Biology, 10, 369-374. Babu, J., Pramod, W.R. & George, T. (2008). Cold active microbial lipases: Some hot issues and recent developments. Biotechnology Advances, 26, 457-470. DOI: 10.1016/j.biotechadv.2008.05.003 Balci, N., Demirel, C. & Kurt, M.A. (2018). Geomicrobiology of Lake Salda and microbial influences on present-day stromatolite formation, Bulletin of the Earth Sciences Application and Research Centre of Hacettepe University, 39, 1, 19-40. Berkner, S., Konradi, S. & Schönfeld, J. (2014). Antibiotic resistance and the environment-there and back again. EMBO Reports, 15, 7, 740-744. DOI: 10.15252/embr.201438978 Bhatt, H.B., Gohel, S.D. & Singh, S.P. (2018). Phylogeny, novel bacterial lineage and enzymatic potential of haloalkaliphilic bacteria from the saline coastal desert of Little Rann of Kutch, Gujarat, India. 3 Biotech, 8, 53, 1-12. DOI: 10.1007/s13205-017-1075-0 Birbir, M., Calli, B., Mertoglu, B., Elevi Bardavid, R., Oren, A., Ogmen, M.N. & Ogan, A. (2007). Extremely halophilic archaea from Tuz Lake, Turkey, and the adjacent Kaldirim and Kayacik salterns. World Journal of Microbiology and Biotechnology, 23, 309-316. DOI: 10.1007/s11274-006-9223-4 Braithwaite, C.J.R. & Zedef, V. (1996). Hydromagnesite stromatolites and sediments in an alkaline lake, Salda Gölü, Turkey. Journal of Sedimentary Research, 66, 991-1002. DOI: 10.1306/D426845F-2B26-11D7-8648000102C1865D Brown, R.W., Sandvik, O., Scherer, R.K. & Rose, D.L. (1969). Differentiation of strains of Staphylococcus epidermidis isolated from bovine udders. Journal of General Microbiology, 47, 273-287. DOI: 10.1306/D426845F-2B26-11D7-8648000102C1865D Caglayan, P., Birbir, M., Sánchez-Porro, C. & Ventosa, A. (2017). Screening of Industrially Important Enzymes Produced by Moderately Halophilic Bacteria Isolated from Salted Sheep Skins of Diverse Origin. Journal of The American Leather Chemists Association, 112(6), 207-216. Caglayan, P., Birbir, M., Sánchez-Porro, C., Ventosa, A. & Birbir, Y. (2018). Investigation of moderately halophilic bacteria causing deterioration of the salted sheep and goat skins and their extermination via electric current applications. Journal of the American Leather Chemists Association, 113, 41-52. Chand, S. & Mishra, P. (2003). Research and application of microbial enzymes, India’s contribution. Advances in Biochemical Engineering/Biotechnology, 85, 95-124. DOI: 10.1007/3-540-36466-8_4. Delgado-García, M., Nicolaus, B., Poli, A., Aguilar, C.N. & Rodríguez-Herrera, R. (2015). Isolation and screening of halophilic bacteria for production of hydrolytic enzymes. In: Maheshwari D, Saraf M (eds) Halophiles, Sustainable Development and Biodiversity, Springer, 396. DOI: 10.1007/978-3-319-14595-2_14 Dunlap, C.A., Schisler, D.A., Perry, E.B., Connor, N., Cohan, F.M. & Rooney, A.P. (2017). Bacillus swezeyi sp. nov. and Bacillus haynesii sp. nov., isolated from desert soil. International Journal of Systematic and Evolutionary Microbiology, 67, 2720-2725. DOI: 10.1099/ijsem.0.002007. Eladli, M.G., Alharbi, N.S., Khaled, J.M., Kadaikunnan, S., Alobaidi, A.S. & Alyahya, S.A. (2019). Antibiotic-resistant Staphylococcus epidermidis isolated from patients and healthy students comparing with antibiotic-resistant bacteria isolated from pasteurized milk. Saudi Journal of Biological Sciences, 26, 6, 1285-1290. DOI: 10.1016/j.sjbs.2018.05.008 EUCAST (The European Committee on Antimicrobial Susceptibility Testing), (2014) Breakpoint Tables for Interpretation of MICs and Zone Diameters, Version 4.0, https://mic.eucast.org/Eucast2/SearchController/search.jsp?action=init, Last accessed date: 10 February 2021. Gillings, M.R. (2013). Evolutionary consequences of antibiotic use for the resistome, mobilome and microbial pangenome. Frontiers in Microbiology, 4, 4, 1-10. DOI: 10.3389/fmicb.2013.00004 Gonzales, C., Gutierrez, C. & Ramirez, C. (1978). Halobacterium vallismortis sp. nov. an amylolytic and carbohydrate-metabolizing extremely halophilic bacterium. Canadian Journal of Microbiology, 24, 710-715. DOI: 10.1139/m78-119. Gupta, R., Beg, Q. & Lorenz, P. (2002). Bacterial alkaline proteases: molecular approaches and industrial applications. Applied Microbiology and Biotechnology, 59, 15-32. DOI: 10.1007/s00253-002-0975-y Harley, J.P. & Prescott, L.M. (2002). Laboratory exercises in microbiology. The McGraw-Hill Companies, New York. Hong, H.A., Khaneja, R., Tam, N.M.K., Cazzato, A., Tan, S., Urdaci, M., Brisson, A., Gasbarrini, A., Barnes, I. & Cutting, S.M. (2009). Bacillus subtilis isolated from the human gastrointestinal tract. Research in Microbiology, 160, 134-143. DOI: 10.1016/j.resmic.2008.11.002. Jaeger, K.E. & Holliger, P. (2010). Chemical biotechnology a marriage of convenience and necessity. Current Opinion in Biotechnology, 21, 711-712. DOI: 10.1016/j.copbio.2010.09.017. Johnson, T.R. & Case, C.L. (2010). Laboratory experiments in microbiology. Pearson Education, San Francisco. Kazanci, N., Girgin, S. & Dügel, M. (2004). On the limnology of Salda Lake, a large and deep soda lake in southwestern Turkey: future management proposals, aquatic conservation. Aquatic Conservation: Marine and Freshwater Ecosystems, 14, 151-162. DOI: 10.1002/aqc.609 Khandeparker, R. & Numan, M.T. (2008). Bifunctional xylanases and their potential use in biotechnology. Industrial Microbiology and Biotechnology, 35, 7, 63-644. DOI: 10.1007/s10295-008-0342-9 Kikani, B.A., Shukla, R.J. & Singh, S.P. (2010). Biocatalytic potential of thermophilic bacteria and actinomycetes. In: Mendez-Vilas A (ed) Current research, technology and education topics in applied microbiology and microbial biotechnology, Formatex Research Center, Badajoz, 1000-1007. Kim, O.S., Cho, Y.J., Lee, K., Yoon, S.H., Kim, M., Na, H., Park, S.C., Jeon, Y.S., Lee, J.H., Yi, H., Won, S. & Chun, J. (2012). Introducing EzTaxon: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. International Journal of Systematic and Evolutionary Microbiology, 62, 716-721. DOI: 10.1099/ijs.0.038075-0. Liu, J., Xu, Y., Nie, Y. & Zhao, G.A. (2012). Optimization production of acid urease by Enterobacter sp. in an approach to reduce urea in Chinese rice wine. Bioprocess and Biosystems Engineering, 35, 651-657. DOI: 10.1007/s00449-011-0643-7
- Liu, Y., Lai, Q. & Dong, C. (2013). Phylogenetic diversity of the Bacillus pumilus group and the marine ecotype revealed by multilocus sequence. Plos One, 8, 11, 1-11. DOI: 10.1371/journal.pone.0080097 Liu, Y., Du, J., Lai, Q., Zeng, R., Ye, D., Xu, J. & Shao, Z. (2017). Proposal of nine novel species of the Bacillus cereus group. International Journal of Systematic and Evolutionary Microbiology, 67, 2499-2508. DOI: 10.1099/ijsem.0.001821 Mirzaagha, P., Louie, M., Sharma, R., Yanke, L.J., Topp, E. & McAllister, T.A. (2011). Distribution and characterization of ampicillin and tetracycline-resistant Escherichia coli from feedlot cattle fed subtherapeutic antimicrobials. BMC Microbiology, 11, 1-15. DOI: 10.1186/1471-2180-11-78 Mitra, P. & Chakrabartty, P.K. (2005). An extracellular protease with depilation activity from Streptomyces nogalator. Journal of Scientific and Industrial Research, 64, 12, 978-983. Nogueira, E.W., Hayash, E.A., Alves, E., Lima, C.A.A., Adorno, M.T. & Brucha, G. (2017). Characterization of alkaliphilic bacteria isolated from Bauxite Residue in the Southern Region of Minas Gerais, Brazil. Brazilian Archives of Biology and Technology, 60, 1-7. DOI: 10.1590/1678-4324-2017160215
- Osman, N.I. & Yin, S. (2018). Isolation and characterization of pea plant (Pisum sativum L.) growth-promoting Rhizobacteria. African Journal of Microbiology Research, 12, 34, 820-828. DOI: 10.5897/AJMR2018.8859 Parvathi, A., Krishna, K., Jose, J., Joseph, N. & Nair, S. (2009). Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India. Brazilian Journal of Microbiology, 40, 269-275. DOI: 10.1590/S1517-838220090002000012 Raval, H., Purohit, M.K. & Singh, S.P. (2015). Extracellular Proteases from Halophilic and Haloalkaliphilic Bacteria: Occurrence and Biochemical Properties. In: D. Maheshwari, M.Saraf (eds) Halophiles, Sustainable Development and Biodiversity, Springer, 421-441. DOI: 10.1007/978-3-319-14595-2_16 Ratnakar, D. (2013). Use of halophile physiology and adaptations in various industrial applications. Research Journal of Biotechnology, 8, 2, 1-3. Rodríguez-Montalvo, R., Vreeland, R.H., Oren, A., Kessel, M. & Lopez, G. (1998). Halogeometricum borinquiense gen. nov. sp. nov. a novel halophilic archeon from Puerto Rico. International Journal of Systematic Bacteriology, 48, 1305-1312. DOI: 10.1099/00207713-48-4-1305 Sambrook, J. & Russell, D.W. (2001). A Laboratory Manual, Cold Spring Harbor, New York, 20-30. Sánchez-Porro, C., Martín, S., Mellado, E. & Ventosa, A. (2003). Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. Journal of Applied Microbiology, 94, 295-300. DOI: 10.1046/j.1365-2672.2003.01834.x Sánchez-Porro, C., Yilmaz, P., De la Haba, R.R., Birbir, M. & Ventosa, A. (2011). Thalassobacillus pellis sp. nov., a moderately halophilic, Gram-positive bacterium isolated from salted hides. International Journal of Systematic and Evolutionary Microbiology, 5, 1206-1210. DOI: 10.1099/ijs.0.024778-0 Shirokova, L.S., Mavromatis, V., Bundeleva, I.A., Pokrovsky, O.S., Benezeth, P., Gerard, E., Pearce, C.R. & Oelkers, E. (2013). Using Mg isotopes to trace cyanobacterially mediated magnesium carbonate precipitation in alkaline lakes. Aquatic Geochemistry, 19, 1-24. DOI: 10.1007/s10498-012-9174-3 Vaishampayan, P., Probst, A., Krishnamurthi, S., Ghosh, S., Osman, S., McDowall, A., Ruckmani, A., Mayilraj, S. & Venkateswaran, K. (2010). Bacillus horneckiae sp. nov., isolated from a spacecraft-assembly clean room. International Journal of Systematic and Evolutionary Microbiology, 60, 1031-1037. DOI: 10.1099/ijs.0.008979-0 Varol, S., Davraz, A., Şener, Ş., Şener, E., Aksever, F., Kırkan, B. & Tokgözlü, A. (2021). Assessment of groundwater quality and usability of Salda Lake Basin (Burdur/Turkey) and health risk related to arsenic pollution. Journal of Environmental Health Science and Engineering, 1-26. DOI: 10.1007/s40201-021-00638-5 Vijayalakshmi, T.M. & Murali, R. (2015). Isolation and screening of Bacillus subtilis isolated from the dairy effluent for the production of protease. International Journal of Current Microbiology and Applied Sciences, 4, 12, 820-827. Wang, C.Y., Hsieh, Y.R., Ng, C.C., Chan, H., Lin, H.T., Tzeng, W.S. & Shyu, Y.T. (2009). Purification and characterization of a novel halostable cellulase from Salinivibrio sp. strain NTU05. Enzyme and Microbial Technology, 44, 373-379. DOI: 10.1016/j.enzmictec.2009.02.006 WHO (World Health Organization). (2017). Critically important antimicrobials for human medicine, ranking of medically important antimicrobials for risk management of antimicrobial resistance due to non-human use, 5th Revision. Geneva, 1-38.
Ayrıntılar
Birincil Dil
İngilizce
Konular
Yapısal Biyoloji , Ekoloji
Bölüm
Araştırma Makalesi
Yazarlar
Pınar Çağlayan
*
0000-0002-0238-1754
Türkiye
Yayımlanma Tarihi
15 Eylül 2021
Gönderilme Tarihi
15 Mart 2021
Kabul Tarihi
25 Mayıs 2021
Yayımlandığı Sayı
Yıl 2021 Cilt: 38 Sayı: 3
APA
Çağlayan, P. (2021). Determination of important enzymes and antimicrobial resistances of gram-positive haloalkaliphilic bacteria isolated from Salda Lake. Ege Journal of Fisheries and Aquatic Sciences, 38(3), 375-382. https://doi.org/10.12714/egejfas.38.3.14
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