A comparative study on antioxidant and cytotoxic effects of Oscillatoria amphibia and Spirulina platensis C-phycocyanin and crude extracts

Burcu Tepedelen Erbaykent; İsmet Gurhan-deliloglu; Meltem Conk Dalay

EN TR

A comparative study on antioxidant and cytotoxic effects of Oscillatoria amphibia and Spirulina platensis C-phycocyanin and crude extracts

Öz

In this study, antioxidant and cytotoxic effects of Oscillatoria amphibia Ege-Macc-14 (Lake Acıgöl-Denizli, Turkey) C-phycocyanin and the crude extract were compared with Spirulina platensis. Since S. platensis is seen as the sole economical source of C-phycocyanin this comparison study may provide novel information for investigating new sources of C-PC and besides new promising cytotoxic compounds. Both species are cultivated under laboratory conditions and both the crude extracts and the C-PCs were obtained. Since O. amphibia has the possibility to produce toxic compounds, in order to figure out the toxicity of the biomass, the aqueous crude extracts of O. amphibia were tested against the brine shrimp directly. The aqueous extracts were found as non-toxic even at the highest concentrations (10 mg.ml-1). Human hepatocellular carcinoma (HEP-3B) cell line was used to investigate the cytotoxic effect of the extracts. The determined IC50 values of S. platensis C-PC, O. amphibia C-PC were 13.27 μg/ml, 4.19 μg/ml respectively and for S. platensis crude extract and O. amphibia crude extract the values were 16.34 μg/ml and 12.94 μg/ml, respectively. The potential antioxidant activity of the crude extracts and the pure C-PCs were evaluated by DPPH free radical, hydroxyl radical scavenging activity assays. As a result, although the pure C-PC of both cyanobacteria showed cytotoxic and antioxidant activities, the highest activity levels were obtained from O. amphibia extracts.

Anahtar Kelimeler

Oscillatoria amphibia ve Spirulina platensis’in C-Fikosiyanin ve ham ekstraktlarının antioksidan ve sitotoksik etkileri üzerine karşılaştırmalı çalışma

Öz

Bu çalışmada Oscillatoria amphibia (Ege Macc-14, Acıgöl-Denizli, Türkiye) anti oksidan ve sitotoksik etkileri, Spirulina platensis’in ham ekstrakt ve fikosiyanini ile karşılaştırılmıştır. S. Platensis’in tek ticari C-fikosiyanin kaynağı olarak görülmesinden dolayı bu çalışma yeni fikosiyanin kaynakları araştırma ve umut vaat eden sitoksik bileşik araştırmalarında yeni veriler sağlayabilir. Her iki tür de laboratuar koşulları altında kültüre alınarak ham ekstrakt ve C-fikosiyanin ekstraksiyonları yapılmıştır. O. amphibia’nın sitokisik bileşikler üretebilme potansiyelinden dolayı biyomasın toksisitesini araştırmak üzere sulu O. amphibia ekstreleri hazırlanmış ve artemialara direkt olarak uygulanmıştır. Sulu ham ekstraktlar en yüksek konsantrasyonda (10 mg.ml-1) bile toksik bulunmamıştır. Ekstraktların sitotoksisitesini belirlemek amacıyla insan karaciğer karsinoma (HEP-3B) hücre hattı kullanılmıştır. S. platensis C-fikosiyanini, O. amphibia Cfikosiyaninleri için IC50 değerleri sırasıyla 13,27 μg/ml ve 4,19 μg/ml olarak hesaplanmış ve bu değerler ham ekstratlar içinde yine sırasıyla 16,34 μg/ml ve 12,94 μg/ml olarak bulunmuştur. Ham ekstrakt ve C-fikosiyaninlerin antioksidan potansiyelleri ise DPPH serbest radikal ve hidroksil süpürme aktivite testleriyle belirlenmiştir. Sonuç olarak her iki siyanobakterinin saf C-fikosiyaninleri sitotoksik ve antioksidan aktivite göstermeleriyle birlikte en yüksek değerler O. amphibia ekstraktları için bulunmuştur

Anahtar Kelimeler

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Not

Yazarlar

Burcu Tepedelen Erbaykent

İsmet Gurhan-deliloglu

Meltem Conk Dalay

Yayımlanma Tarihi

1 Mart 2012

Gönderilme Tarihi

7 Aralık 2015

Kabul Tarihi

-

Yayımlandığı Sayı

Yıl 2012 Cilt: 29 Sayı: 1

IZ

https://izlik.org/JA68WG73HC

Kaynak Göster

RIS / Bibtex

APA

Erbaykent, B. T., Gurhan-deliloglu, İ., & Dalay, M. C. (2012). A comparative study on antioxidant and cytotoxic effects of Oscillatoria amphibia and Spirulina platensis C-phycocyanin and crude extracts. Ege Journal of Fisheries and Aquatic Sciences, 29(1), 1-7. https://izlik.org/JA68WG73HC

AMA

1.Erbaykent BT, Gurhan-deliloglu İ, Dalay MC. A comparative study on antioxidant and cytotoxic effects of Oscillatoria amphibia and Spirulina platensis C-phycocyanin and crude extracts. EgeJFAS. 2012;29(1):1-7. https://izlik.org/JA68WG73HC

Chicago

Erbaykent, Burcu Tepedelen, İsmet Gurhan-deliloglu, ve Meltem Conk Dalay. 2012. “A comparative study on antioxidant and cytotoxic effects of Oscillatoria amphibia and Spirulina platensis C-phycocyanin and crude extracts”. Ege Journal of Fisheries and Aquatic Sciences 29 (1): 1-7. https://izlik.org/JA68WG73HC.

EndNote

Erbaykent BT, Gurhan-deliloglu İ, Dalay MC (01 Mart 2012) A comparative study on antioxidant and cytotoxic effects of Oscillatoria amphibia and Spirulina platensis C-phycocyanin and crude extracts. Ege Journal of Fisheries and Aquatic Sciences 29 1 1–7.

IEEE

[1]B. T. Erbaykent, İ. Gurhan-deliloglu, ve M. C. Dalay, “A comparative study on antioxidant and cytotoxic effects of Oscillatoria amphibia and Spirulina platensis C-phycocyanin and crude extracts”, EgeJFAS, c. 29, sy 1, ss. 1–7, Mar. 2012, [çevrimiçi]. Erişim adresi: https://izlik.org/JA68WG73HC

ISNAD

Erbaykent, Burcu Tepedelen - Gurhan-deliloglu, İsmet - Dalay, Meltem Conk. “A comparative study on antioxidant and cytotoxic effects of Oscillatoria amphibia and Spirulina platensis C-phycocyanin and crude extracts”. Ege Journal of Fisheries and Aquatic Sciences 29/1 (01 Mart 2012): 1-7. https://izlik.org/JA68WG73HC.

JAMA

1.Erbaykent BT, Gurhan-deliloglu İ, Dalay MC. A comparative study on antioxidant and cytotoxic effects of Oscillatoria amphibia and Spirulina platensis C-phycocyanin and crude extracts. EgeJFAS. 2012;29:1–7.

MLA

Erbaykent, Burcu Tepedelen, vd. “A comparative study on antioxidant and cytotoxic effects of Oscillatoria amphibia and Spirulina platensis C-phycocyanin and crude extracts”. Ege Journal of Fisheries and Aquatic Sciences, c. 29, sy 1, Mart 2012, ss. 1-7, https://izlik.org/JA68WG73HC.

Vancouver

1.Burcu Tepedelen Erbaykent, İsmet Gurhan-deliloglu, Meltem Conk Dalay. A comparative study on antioxidant and cytotoxic effects of Oscillatoria amphibia and Spirulina platensis C-phycocyanin and crude extracts. EgeJFAS [Internet]. 01 Mart 2012;29(1):1-7. Erişim adresi: https://izlik.org/JA68WG73HC