Antibacterial activity of some essential oils againts Vagococcus salmoninarum

Seçil Metin; Zühre İşıl Biçer

doi:10.12714/egejfas.37.2.07

TR EN

Vagococcus salmoninarum’a karşı bazı uçucu yağların antibakteriyel aktivitesi

Öz

Bu çalışmada, kekik (Origanum vulgare), sarı kantaron (Hypericum perforatum), biberiye (Rosmarinus officinalis), zencefil (Zingiber officinale), karanfil (Eugenia caryophyllata), nane (Menta piperita), lavanta (Lavandula hybrida) ve çörek otu (Nigella sativa)’dan elde edilen uçucu yağların kimyasal bileşimleri ve Vagococcus salmoninarum’a karşı in vitro antibakteriyal etkileri incelenmiştir. Uçucu yağların kimyasal kompozisyonu Gaz Kromatografisi/Kütle Spektrometresi (GC/MS) ile analizlenmiştir. Vagococcus salmoninarum’a karşı uçucu yağların antibakteriyel etkileri agar difüzyon ve tüp dilüsyon yöntemi ile belirlenmiştir. Kekik (125 μL/ml MİK) ve karanfil (500 μL/ml MİK) uçucu yağlarının V. salmoninarum’a karşı güçlü antibakteriyel etkinliğe sahip olduğu görülmüştür. Etki gösteren uçucu yağlarının ana bileşenleri sırasıyla karvakrol (%63,57) ve eugenol (%85,99) olarak tespit edilmiştir. Sonuç olarak, vagokokkozis hastalığı etkeni V. salmoninarum’a karşı test edilen iki bitki türünün de antibakteriyel ajan olarak kullanılabileceği düşünülmektedir. Bu amaçla ilerki çalışmalarda, kekik ve karanfil uçucu yağlarının vagokokkozise karşı in vivo antibakteriyel etkinlikleri araştırılmalıdır.

Anahtar Kelimeler

Antibakteriyal aktivite,Vagococcus salmoninarum,uçucu yağ,agar difüzyon,minimum inhibitör konsantrasyon (MİK)

Antibacterial activity of some essential oils againts Vagococcus salmoninarum

Abstract

In this study, essential oils of thyme (Origanum vulgare), St. John's Wort (Hypericum perforatum), rosemary (Rosmarinus officinalis), ginger (Zingiber officinale), clove (Eugenia caryophyllata), peppermint (Menta piperita), lavandula (Lavandula hybrida) and black cumin (Nigella sativa) were screened for its chemical composition and in vitro antibacterial activity against Vagococcus salmoninarum. The composition of oils were analysed using GC/MS. Antibacterial effects of essential oils against V. salmoninarum were detected by agar diffusion and tube dilution assays. Thyme (125 μL ml^-1 MIC) and clove (500 μL ml^-1 MIC) essential oils were shown to possess strong antibacterial activity against V. salmoninarum. The main components of effective essential oils were carvacrol (63.57 %) and eugenol (85.99%), respectively. As a result, these two plant species that tested against V. salmoninarum which is causative agent of vagococcosis can be used as an antibacterial agent. For this purpose, in vivo antibacterial activities against vagococcosis of thyme and clove essential oils should be explored in the further studies.

Keywords

Antibacterial activity,Vagococcus salmoninarum,essential oil,agar diffusion,minimal inhibitory concentration (MIC)

Destekleyen Kurum

Suleyman Demirel University, Scientific Research Project Grant.

Proje Numarası

SDU BAP 4031-YL1-14

Teşekkür

This study was financially supported by Suleyman Demirel University, Scientific Research Project Grant ( SDU BAP 4031-YL1-14).

Kaynakça

Abutbul, S., Golan-Goldhirsh, A., Barazani, O., Ofir, R., & Zilberg, D. (2005). Screening of desert plants for use against bacterial pathogens in fish. Israeli Journal of Aquaculture- Bamidgeh, 57(2): 71-80.
Al Laham, S. A., & Al Fadel, F. M. (2014). Antibacterial activity of various plants extracts against antibiotic-resistant Aeromonas hydrophila. Jundishapur Journal of Microbiology, 7(7):1-7.
Bansemir, A., Blume, M., Schröder, S., & Lindequist, U. (2006). Screening of cultivated seaweeds for antibacterial activity against fish pathogenic bacteria. Aquaculture, 252(1), 79-84.
Baydar, H., Sağdiç, O., Özkan, G., & Karadoğan, T. (2004). Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control, 15(3), 169-172.
Chaieb, K., Hajlaoui, H., Zmantar, T., Kahla‐Nakbi, A. B., Rouabhia, M., Mahdouani, K., & Bakhrouf, A. (2007). The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata (Syzigium aromaticum L. Myrtaceae): a short review. Phytotherapy Research, 21(6), 501-506.
Citarasu, T. (2010). Herbal biomedicines: a new opportunity for aquaculture industry. Aquaculture International, 18(3), 403-414.
Cleff, M. B., Meinerz, A. R., Xavier, M., Schuch, L. F., Meireles, M. C. A., Rodrigues, M. R. A., & Mello, J. R. B. D. (2010). In vitro activity of Origanum vulgare essential oil against Candida species. Brazilian Journal of Microbiology, 41(1), 116-123.
De Martino, L., De Feo, V., Formisano, C., Mignola, E., & Senatore, F. (2009). Chemical composition and antimicrobial activity of the essential oils from three chemotypes of Origanum vulgare L. ssp. hirtum (Link) Ietswaart growing wild in Campania (Southern Italy). Molecules, 14(8), 2735-2746.

Didinen, B. I., Kubilay, A., Diler, O., Ekici, S., Onuk, E. E., & Findik, A. (2011). First isolation of Vagococcus salmoninarum from cultured rainbow trout (Oncorhynchus mykiss, Walbaum) broodstocks in Turkey. Bulletin of the European Association of Fish Pathologists, 31(6), 235-243.
Diler, O., Gormez, O., Diler, I., & Metin, S. (2017a). Effect of oregano (Origanum onites L.) essential oil on growth, lysozyme and antioxidant activity and resistance against Lactococcus garvieae in rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture Nutrition, 23(4), 844-851.
Diler, Ö., Görmez, Ö., Metin, S., İlhan, İ., & Diler, İ. (2017b). Origanum vulgare L. uçucu yağının gökkuşağı alabalıkları (Oncorhyncus mykiss)’nda büyüme, lizozim ve antioksidan aktivite ve Vibrio anguillarum’a karşı direnç üzerine etkisi. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 13(1), 42-57.
Ekici, S., Diler, Ö., Didinen, B. I., & Kubilay, A. (2011). Antibacterial activity of essential oils from medicinal plants against bacterial fish pathogens. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17(Supplement A): 47-54.
Eldar, A. A., & Ghittino, C. (1999). Lactococcus garvieae and Streptococcus iniae infections in rainbow trout Oncorhynchus mykiss: similar, but different diseases. Diseases of aquatic organisms, 36(3), 227-231.Ghittino, C., Accornero, P., Prearo, M., Rogato, F., Zlotkin, A., & Eldar, A. (1999). Coldwater streptococcoses in salmonids, with particular reference to Vagococcus salmoninarum infection. Proceedings of Workshop in Fish Streptococcoses, IZS – State Veterinary Institute, Turin, Italy, 3.
Ghittino, C., Agnetti, F., Panzieri, C., Cabra, S., Colussi, S., & Prearo, M. (2004). L’infezione da Vagococcus salmoninarum della trota iridea in Italia. Ittiopatologia, 1, 25-33.
Ghittino, C., Latini, M., Agnetti, F., Panzieri, C., Lauro, L., Ciappelloni, R., & Petracca, G. (2003). Emerging pathologies in aquaculture: effects on production and food safety. Veterinary Research Communications, 27(1), 471-479.
Goliaris, A. H., Chatzopoulou, P. S., & Katsiotis, S. T. (2003). Production of new Greek oregano clones and analysis of their essential oils. Journal of Herbs, Spices & Medicinal Plants, 10(1), 29-35.
Guan, W., Li, S., Hou, C., Yan, R., & Ma, J. (2007). Determination and correlation of solubilities of clove oil components in supercritical carbon dioxide. Journal of Chemical Industry and Engineering, 58: 1077-1081.
Hammer, K.A., Carson, C.F., & Riley, T.V. (1999). Antimicrobial activity of essential oils and other plant extracts. The Journal of Applied Microbiology, 86(6): 985-990.
Haniffa, M.A., & Kavitha, K. (2012). Antibacterial activity of medicinal herbs against the fish pathogen Aeromonas hydrophila. Journal of Agricultural Technology, 8(1): 205-211.
Kara, N., & Baydar, H. (2013). Determination of lavender and lavandin cultivars (Lavandula sp.) containing high quality essential oil in Isparta, Turkey. Turkish Journal of Field Crops, 18(1): 58-65.
Kumar, P., Mishra, S., Malik, A., & Satya, S. (2011). Insecticidal properties of Mentha species: a review. Industrial Crops and Products, 34(1): 802-817.
Metin, S., Didinen, B.I., Mercimek, E.B., & Ersoy, A.T. (2017). Antibacterial Activity of Some Essential Plant Oils Againts Some Bacterial Fish Pathogens. Aquaculture Studies, 17(1): 059-069.
Michel, C., Nougayrede, P., Eldar, A., Sochon, E., & De Kinkelin, P. (1997). Vagococcus salmoninarum, a bacterium of pathological signiﬁcance in rainbow trout Oncorhynchus mykiss farming. Diseases of Aquatic Organisms, 30: 199–208.
Mılos, M., Mastelic, J., & Jerkovic, I. (2000). Chemical composition and antioxidant effect of glycosidically bound volatile compounds from Oregano (Origanum vulgare L. ssp. hirtum). Food Chemistry, 71: 79-83.
NCCLS, (2001). Testing; Eleventh Information Supplement. NCCLS document M100-S11 NCCLS, Pennsylvania, USA.
Noori, S., Zeynali, F., & Alması, H. (2018). Antimicrobial and antioxidant efficiency of nanoemulsion-based edible coating containing ginger (Zingiber officinale) essential oil and its effect on safety and quality attributes of chicken breast fillets. Food Control, 84: 312-320.
Öntaş, C., Baba, E., & Kaplaner, E. (2016). Antibacterial activity of Citrus limon peel essential oil and Argania spinosa oil against fish pathogenic bacteria. Journal of the Faculty of Veterinary Medicine, Kafkas University, 22: 741-749.
Özay, C., Ulukoy, G., Mammadov, R., & Sayın, Z. (2018). Radical scavenging activity and antibacterial effect of three cyclamen l. tuber extracts on some fish pathogens. SDÜ Journal of Natural and Applied Sciences, 22(2): 562-568.
Park, J.W., Wendt, M., & Heo, G.J. (2016). Antimicrobial activity of essential oil of Eucalyptus globulus against fish pathogenic bacteria. Laboratory Animal Research, 32(2): 87-90.
Prashar, A., Locke, I.C., & Evans, C.S. (2006). Cytotoxicity of clove (Syzygium aromaticum) oil and its major components to human skin cells. Cell Proliferation, 39: 241–248.
Radusıene, J., Judþıntıenë, A., Peèıulytë, D., & Janulıs, V. (2005). Chemical composition of essential oil and antimicrobial activity of Origanum vulgare. Biologija, 4: 53-58.
Raına, A.P., & Negi, K.S. (2014). Chemical diversity among different accessions of Origanum vulgare L. ssp. vulgare collected from Central Himalayan region of Uttarakhand, India. Journal of Essential Oil Research, 26(6): 420-426.
Ribeiro-Santos, R., Carvalho-Costa, D., Cavaleiro, C., Costa, H.S., Albuquerque, T.G., Castilho, M.C., Ramos, F., Melo, M.R., & Sanches-Silva, A. (2015). A novel insight on an ancient aromatic plant: The rosemary (Rosmarinus officinalis L.). Trends in Food Science & Technology, 45(2): 355-368.
Ruız-Zarzuela, I., De Blas, I., Girones, O., Ghittino, C., & Muzquız, JL. (2005). Isolation of Vagococcus salmoninarum in rainbow trout Oncorhynchus mykiss (Walbaum), broodstocks: characterisation of the pathogen. Veterinary Research Communications, 29: 553–562.
Salogni, C., Perantoni, P., Pitozzi, A., Loris, G., & Alboralı, G.L. (2007). Vagococcus salmoninarum: descrizione di un focolaio di malattia in riproduttori di trota iridea (Oncorhynchus mykiss). Ittiopatologia, 4: 59–66.
Schmidtke, L.N., & Carson, J. (1994). Characteristics of Vagococcus salmoninarum isolated from diseased salmonid ﬁsh. Journal of Applied Bacteriology, 77: 229–236.
Souza, E.L., Barros, J.C., Oliveira, C.E.V., & Conceicao, M.L. (2008). Influence of Origanum vulgare L. essential oil on enterotoxin production, membrane permeability and surface characteristics of Staphylococcus aureus. The International Journal of Food Microbiology, 137(2-3): 308-311.
Tanrıkul, T., Avsever, M.L., Onuk, E.E., & Didinen, B.I. (2014). Vagococcus salmoninarum, a causative agent of disease in rainbow trout (Oncorhynchus mykiss, Walbaum) broodstocks in the aegean region of Turkey. Etlik Veterinary Microbiology Journal, 25:11–16.
Teixeira, B., Marques, A., Ramos, C., Serrano, C., Matos, O., Neng, N.R., Nogueıra, J.M.F., Saraıva, J.A., & Nunes, M.L. (2013). Chemical composition and bioactivity of different oregano (Origanum vulgare) extracts and essential oil. The Journal of the Science of Food and Agriculture, 93: 2707–2714.
Turker, H., & Yıldırım, A.B. (2015). Screening for antibacterial activity of some Turkish plants against fish pathogens: a possible alternative in the treatment of bacterial infections. Biotechnology & Biotechnological Equipment, 29(2): 281-288.
Vıuda-Martos, M., Ruız-Navajas, Y., Fernandez-Lopez, J., & Perez-Alverez, J.A. (2007). Chemical composition of the essential oils obtained from some spices widely used in mediterranean region. Acta Chimica Slovenica, 54: 921-926.
Xu, J.G., Lıu, T., Hu, Q.P., & Cao, X.M. (2016). Chemical composition, antibacterial properties and mechanism of action of essential oil from clove buds against Staphylococcus aureus. Molecules, 21(9): 1194.

Ayrıntılar

Birincil Dil

İngilizce

Konular

Balıkçılık Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Seçil Metin ^*
0000-0002-8029-5624
Türkiye

Zühre İşıl Biçer
0000-0003-3669-5214
Türkiye

Yayımlanma Tarihi

15 Haziran 2020

Gönderilme Tarihi

18 Ekim 2019

Kabul Tarihi

24 Aralık 2019

Yayımlandığı Sayı

Yıl 2020 Cilt: 37 Sayı: 2

DOI

https://doi.org/10.12714/egejfas.37.2.07

IZ

https://izlik.org/JA26SK42EH

Kaynak Göster

RIS / Bibtex

APA

Metin, S., & Biçer, Z. İ. (2020). Antibacterial activity of some essential oils againts Vagococcus salmoninarum. Ege Journal of Fisheries and Aquatic Sciences, 37(2), 167-173. https://doi.org/10.12714/egejfas.37.2.07

Cited By

Hyperıcum perforatum L. esansiyel yağının in vitro antimikrobiyal, antioksidan aktivite ve kimyasal karakterizasyonu

Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi

https://doi.org/10.17474/artvinofd.702853

$Foeniculum$ $vulgare$ ve $Origanum$ $onites$ Uçucu Yağ Nanoemülsiyonlarının Bakteriyel Balık Patojenlerine Karşı Antibakteriyel ve Antibiyofilm Aktivitesinin Tespiti

Sinop Üniversitesi Fen Bilimleri Dergisi

https://doi.org/10.33484/sinopfbd.1558579

In vitro Antimicrobial Effect of Oregano, Garlic, and Rosemary Essential Oil Combinations Against Fish Pathogens

Journal of Anatolian Environmental and Animal Sciences

https://doi.org/10.35229/jaes.1813438