Antibacterial activity of some essential oils againts Vagococcus salmoninarum

Seçil Metin; Zühre İşıl Biçer

doi:10.12714/egejfas.37.2.07

TR EN

Vagococcus salmoninarum’a karşı bazı uçucu yağların antibakteriyel aktivitesi

Öz

Bu çalışmada, kekik (Origanum vulgare), sarı kantaron (Hypericum perforatum), biberiye (Rosmarinus officinalis), zencefil (Zingiber officinale), karanfil (Eugenia caryophyllata), nane (Menta piperita), lavanta (Lavandula hybrida) ve çörek otu (Nigella sativa)’dan elde edilen uçucu yağların kimyasal bileşimleri ve Vagococcus salmoninarum’a karşı in vitro antibakteriyal etkileri incelenmiştir. Uçucu yağların kimyasal kompozisyonu Gaz Kromatografisi/Kütle Spektrometresi (GC/MS) ile analizlenmiştir. Vagococcus salmoninarum’a karşı uçucu yağların antibakteriyel etkileri agar difüzyon ve tüp dilüsyon yöntemi ile belirlenmiştir. Kekik (125 μL/ml MİK) ve karanfil (500 μL/ml MİK) uçucu yağlarının V. salmoninarum’a karşı güçlü antibakteriyel etkinliğe sahip olduğu görülmüştür. Etki gösteren uçucu yağlarının ana bileşenleri sırasıyla karvakrol (%63,57) ve eugenol (%85,99) olarak tespit edilmiştir. Sonuç olarak, vagokokkozis hastalığı etkeni V. salmoninarum’a karşı test edilen iki bitki türünün de antibakteriyel ajan olarak kullanılabileceği düşünülmektedir. Bu amaçla ilerki çalışmalarda, kekik ve karanfil uçucu yağlarının vagokokkozise karşı in vivo antibakteriyel etkinlikleri araştırılmalıdır.

Anahtar Kelimeler

Antibakteriyal aktivite,Vagococcus salmoninarum,uçucu yağ,agar difüzyon,minimum inhibitör konsantrasyon (MİK)

Antibacterial activity of some essential oils againts Vagococcus salmoninarum

Abstract

In this study, essential oils of thyme (Origanum vulgare), St. John's Wort (Hypericum perforatum), rosemary (Rosmarinus officinalis), ginger (Zingiber officinale), clove (Eugenia caryophyllata), peppermint (Menta piperita), lavandula (Lavandula hybrida) and black cumin (Nigella sativa) were screened for its chemical composition and in vitro antibacterial activity against Vagococcus salmoninarum. The composition of oils were analysed using GC/MS. Antibacterial effects of essential oils against V. salmoninarum were detected by agar diffusion and tube dilution assays. Thyme (125 μL ml^-1 MIC) and clove (500 μL ml^-1 MIC) essential oils were shown to possess strong antibacterial activity against V. salmoninarum. The main components of effective essential oils were carvacrol (63.57 %) and eugenol (85.99%), respectively. As a result, these two plant species that tested against V. salmoninarum which is causative agent of vagococcosis can be used as an antibacterial agent. For this purpose, in vivo antibacterial activities against vagococcosis of thyme and clove essential oils should be explored in the further studies.

Keywords

Antibacterial activity,Vagococcus salmoninarum,essential oil,agar diffusion,minimal inhibitory concentration (MIC)

Supporting Institution

Suleyman Demirel University, Scientific Research Project Grant.

Project Number

SDU BAP 4031-YL1-14

Thanks

This study was financially supported by Suleyman Demirel University, Scientific Research Project Grant ( SDU BAP 4031-YL1-14).

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Details

Primary Language

English

Subjects

Fisheries Management

Journal Section

Research Article

Authors

Seçil Metin ^*
0000-0002-8029-5624
Türkiye

Zühre İşıl Biçer
0000-0003-3669-5214
Türkiye

Publication Date

June 15, 2020

Submission Date

October 18, 2019

Acceptance Date

December 24, 2019

Published in Issue

Year 2020 Volume: 37 Number: 2

DOI

https://doi.org/10.12714/egejfas.37.2.07

IZ

https://izlik.org/JA26SK42EH

Cite

RIS / Bibtex

APA

Metin, S., & Biçer, Z. İ. (2020). Antibacterial activity of some essential oils againts Vagococcus salmoninarum. Ege Journal of Fisheries and Aquatic Sciences, 37(2), 167-173. https://doi.org/10.12714/egejfas.37.2.07

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