Araştırma Makalesi

Evaluation of DNA damage by Comet Assay in populations of endemic Beyşehir frog Pelophylax caralitanus (Arıkan,1988)

Cilt: 36 Sayı: 2 15 Haziran 2019
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Evaluation of DNA damage by Comet Assay in populations of endemic Beyşehir frog Pelophylax caralitanus (Arıkan,1988)

Abstract



In study this, the level of DNA damage in three populations of endemic Beyşehir frog (Pelophylax caralitanus) inhabiting on Lakes of Karamık, Eber and Beyşehir with different anthropogenic pollution was assessed by using the DNA Comet Assay technique. Frog erythrocytes cells were used for the analysis. Seven adult individuals were collected from each biotope containing minimum 100 cells were analysed. While the results showing a significant increase in the DNA comet index in the contaminated zone [(Eber Lake= Idc=0.22±0.015), (p<0.05)], the majority of the cells did not contain any DNA damage in the clean zone (Karamık Lake, Idc=0.08±0.001). The obtained data demonstrated t that the endemic Beyşehir frog could serve as a useful flag species in an indicator of anthropogenic impact on ecosystems.




Keywords

Destekleyen Kurum

TÜBİTAK - Afyon Kocatepe University

Proje Numarası

KBAG 113Z139 - 16.FENED.07

Teşekkür

This work was supported by the Scientific and Technological Research Council of Turkey (TÜBİTAK), Grant No:KBAG 113Z139 and Afyon Kocatepe University Research Funds Grant No:16.FENED.07

Kaynakça

  1. Arıkan, H. (1988). On a new form Rana ridibunda (Anura: Ranidae) from Turkey. Istanbul Üniversitesi Fen Fakültesi Mecmuası, İstanbul, 53, 81-87.
  2. Ashraf, W. (2005). Accumulation of heavy metals in kidney and heart tissues of Epinephelus microdon fish from the Arabian Gulf, Environmental Monitoring and Assessment. 101, 311-316. DOI:10.1007/s10661-005-0298-4.
  3. Budak, A., Tok, C.V. & Ayaz, D. (2000). On specimens of Rana ridibunda Pallas, 1771 (Anura: Ranidae) collected from Işıklı Lake (Çivril-Denizli). Turkish Journal of Zoology, 24, 135-137.
  4. Cotelle, S. & Férard, J.F. (1999). Comet assay in genetic ecotoxicology, A review. Environmental and Molecular Mutagenesis, 34, 246-255. DOI:10.1002/(SICI)1098-2280.
  5. Dautremepuits, C., Paris-Palacios, S., Betoulle, S. & Vernet G. (2004). Modulation in hepatic and head kidney parameters of Carp (Cyprinus carpio) induced by copper and chitosan. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 137, 325-333.
  6. Devaux, A., Flammarion, P. & Bernardon, V. (1998). Monitoring of the chemical pollution of the river Rhonethrough measurement of DNA damage and cytochrome P-450IA induction in chub (Leuciscus cephalus). Marine Environmental Research, 46, 257-262. DOI:10.1016/S0141-1136(97)00105.
  7. Dhawan, A., Bajpayee, M. & Parmar, D. (2009). Comet assay: a reliable tool for the assessment of DNA damage in different models. Cell Biology and Toxicology, 25, 5-32. DOI:10.1007/s10565-008-9072.
  8. El-Zein, R.A., Hastings-Smith, D.A., Ammenheuser, M.M., Trinen-Moslen, M., Gulland, F.M. & Ward, J.B. (2006). Evaluation of two different biomarkers for use in the assessment of toxic chemical exposure in California sea lions (Zalophus californianus). Marine Pollution Bulletin, 52, 104-120. DOI:10.1016/j.marpolbul. 2005.10.007.

Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Araştırma Makalesi

Yayımlanma Tarihi

15 Haziran 2019

Gönderilme Tarihi

30 Ekim 2018

Kabul Tarihi

23 Ocak 2019

Yayımlandığı Sayı

Yıl 2019 Cilt: 36 Sayı: 2

Kaynak Göster

APA
Erişmiş, U. C. (2019). Evaluation of DNA damage by Comet Assay in populations of endemic Beyşehir frog Pelophylax caralitanus (Arıkan,1988). Ege Journal of Fisheries and Aquatic Sciences, 36(2), 109-114. https://doi.org/10.12714/egejfas.2019.36.2.02