Evaluation of DNA damage by Comet Assay in populations of endemic Beyşehir frog Pelophylax caralitanus (Arıkan,1988)

Uğur Cengiz Erişmiş

doi:10.12714/egejfas.2019.36.2.02

TR EN

Evaluation of DNA damage by Comet Assay in populations of endemic Beyşehir frog Pelophylax caralitanus (Arıkan,1988)

Abstract

In study this, the level of DNA damage in three populations of endemic Beyşehir frog (Pelophylax caralitanus) inhabiting on Lakes of Karamık, Eber and Beyşehir with different anthropogenic pollution was assessed by using the DNA Comet Assay technique. Frog erythrocytes cells were used for the analysis. Seven adult individuals were collected from each biotope containing minimum 100 cells were analysed. While the results showing a significant increase in the DNA comet index in the contaminated zone [(Eber Lake= Idc=0.22±0.015), (p<0.05)], the majority of the cells did not contain any DNA damage in the clean zone (Karamık Lake, Idc=0.08±0.001). The obtained data demonstrated t that the endemic Beyşehir frog could serve as a useful flag species in an indicator of anthropogenic impact on ecosystems.

Keywords

Pelophylax caralitanus,DNA damage,anthropogenic impact,endemic Beyşehir frog,comet assay

Supporting Institution

TÜBİTAK - Afyon Kocatepe University

Project Number

KBAG 113Z139 - 16.FENED.07

Thanks

This work was supported by the Scientific and Technological Research Council of Turkey (TÜBİTAK), Grant No:KBAG 113Z139 and Afyon Kocatepe University Research Funds Grant No:16.FENED.07

References

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Uğur Cengiz Erişmiş ^*
0000-0002-1288-3088
Türkiye

Publication Date

June 15, 2019

Submission Date

October 30, 2018

Acceptance Date

January 23, 2019

Published in Issue

Year 2019 Volume: 36 Number: 2

DOI

https://doi.org/10.12714/egejfas.2019.36.2.02

IZ

https://izlik.org/JA94MN96UU

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RIS / Bibtex

APA

Erişmiş, U. C. (2019). Evaluation of DNA damage by Comet Assay in populations of endemic Beyşehir frog Pelophylax caralitanus (Arıkan,1988). Ege Journal of Fisheries and Aquatic Sciences, 36(2), 109-114. https://doi.org/10.12714/egejfas.2019.36.2.02

Evaluation of DNA damage by Comet Assay in populations of endemic Beyşehir frog Pelophylax caralitanus (Arıkan,1988)

Endemik Beyşehir kurbağası Pelophylax caralitanus (Arıkan,1988) populasyonlarında Comet Analizi ile DNA hasarın değerlendirilmesi

Öz

Anahtar Kelimeler

Evaluation of DNA damage by Comet Assay in populations of endemic Beyşehir frog Pelophylax caralitanus (Arıkan,1988)

Abstract

Keywords

Supporting Institution

Project Number

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite