Research Article
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Expression of cytochrome P450 aromatase isoforms in female Alburnus tarichi (Guldenstaedtii, 1814)

Year 2021, Volume: 38 Issue: 2, 247 - 252, 15.06.2021

Güler Ünal Emily Marquez Mara O'brien Pericles Stavropoulos Ian P. Callard

https://doi.org/10.12714/egejfas.38.2.15

Abstract


In this study, we aimed to clone brain-derived Cyp19b and ovary-derived Cyp19a the P450 aromatase gene isoforms and to indicate the expression levels of these genes in the hypothalamus and ovary tissues from reproductively arrested ovarian development (RA) and non-arrested ovarian development (RN) Alburnus tarichi from Lake Van, Turkey. The expression levels of Cyp19b and Cyp19a genes were predominant in the hypothalamus and ovary, respectively. The level of Cyp19b mRNA was significantly lower in the hypothalamus and ovary from RA fish than in the hypothalamus and ovary from RN fish (P<0.05). The expression level of Cyp19a was significantly lower in the ovary of RA fish (P<0.05) than RN fish while no difference was found in the hypothalamus of both RA and RN fish (P<0.05). According to these findings, we suggest that the RA fish represent a segment of the population and these fish may be more sensitive to endocrine disruption compound/s than others.

Keywords

P450 aromatase gene expression Alburnus tarichi Lake Van

Supporting Institution

This work was supported by National Institue of Environmental Health Sciences (NIEHS)

Project Number

NIEHS-07381

Thanks

This work was supported by National Institue of Environmental Health Sciences (NIEHS) 07381 to Dr. Ian P. Callard. This work was supported by National Institue of Environmental Health Sciences (NIEHS) 07381 to Dr. Ian P. Callard. We want to thank Kellie A. Cotter and Rebecca Meyer for technical advice, and Burak Kaptaner, Ertugrul Kankaya and Ahmet R. Oğuz for fish sampling.

References

  • Barney, M. L., Patil, J. G., Gunasekera, R. M., & Carter, C. G. (2008). Distinct cytochrome P450 aromatase isoforms in the common carp (Cyprinus carpio): Sexual dimorphism and onset of ontogenic expression. General and Comparative Endocrinology, 156(3), 499-508. DOI:10.1016/j.ygcen.2008.03.013
  • Callard, G. V., Tchoudakova, A. V., Kishida, M., & Wood, E. (2001). Differential tissue distribution, developmental programming, estrogen regulation and promoter characteristics of cyp19 genes in teleost fish. The Journal of Steroid Biochemistry and Molecular Biology, 79(1-5), 305-314. DOI:10.1016/S0960-0760(01)00147-9
  • Chang, X., Kobayashi, T., Senthilkumaran, B., Kobayashi-Kajura, H., Sudhakumari, C. C., & Nagahama, Y. (2005). Two types of aromatase with different encoding genes, tissue distribution and developmental expression in Nile tilapia (Oreochromis niloticus). General and Comparative Endocrinology, 141(2), 101-115. DOI:10.1016/j.ygcen.2004.11.020
  • Cheshenko, K., Brion, F., Le Page, Y., Hinfray, N., Pakdel, F., Kah, O., … Eggen, R. I. (2006). Expression of zebra fish Aromatase cyp19a and cyp19b genes in response to the ligands of estrogen receptor and aryl hydrocarbon receptor. Toxicological Sciences, 96(2), 255-267. DOI:10.1093/toxsci/kfm003
  • Cheshenko, K., Pakdel, F., Segner, H., Kah, O., & Eggen, R. I. (2008). Interference of endocrine disrupting chemicals with aromatase CYP19 expression or activity, and consequences for reproduction of teleost fish. General and Comparative Endocrinology, 155(1), 31-62. DOI:10.1016/j.ygcen.2007.03.005
  • Chiang, E. F., Yan, Y., Guiguen, Y., Postlethwait, J., & Chung, B. (2001). Two Cyp19 (P450 Aromatase) genes on duplicated Zebrafish chromosomes are expressed in ovary or brain. Molecular Biology and Evolution, 18(4), 542-550. DOI:10.1093/oxfordjournals.molbev.a003833
  • Dong, W., & Willett, K. L. (2008). Local expression of CYP19A1 and CYP19A2 in developing and adult killifish (Fundulus heteroclitus). General and Comparative Endocrinology, 155(2), 307-317. DOI:10.1016/j.ygcen.2007.05.018
  • EPA (2001). Sampling procedures for the 2001 National Sewage Sludge Survey. Office of Science and Technology Washington, D.C. www.epa.gov.
  • Fenske, M., & Segner, H. (2004). Aromatase modulation alters gonadal differentiation in developing zebrafish (Danio rerio). Aquatic Toxicology, 67(2), 105-126. DOI: 10.1016/j.aquatox.2003.10.008
  • Forlano, P. M., Deitcher, D. L., Myers, D. A., & Bass, A. H. (2001). Anatomical distribution and cellular basis for high levels of Aromatase activity in the brain of teleost fish: Aromatase enzyme and mRNA expression identify glia as source. The Journal of Neuroscience, 21(22), 8943-8955. DOI:10.1523/JNEUROSCI.21-22-08943.2001
  • Gelinas, D., A. Pitoc, G., & V. Callard, G. (1998). Isolation of a goldfish brain cytochrome P450 aromatase cDNA:. Molecular and Cellular Endocrinology, 138(1-2), 81-93. DOI: 10.1016/S0303-7207(98)00015-X
  • Greytak, S. R., Champlin, D., & Callard, G. V. (2005). Isolation and characterization of two cytochrome P450 aromatase forms in killifish (Fundulus heteroclitus): Differential expression in fish from polluted and unpolluted environments. Aquatic Toxicology, 71(4), 371-389. DOI:10.1016/j.aquatox.2004.12.007
  • Kazeto, Y., Goto-Kazeto, R., Place, A., & Trant, J. (2003). Aromatase expression in zebrafish and channel catfish brains: Changes in transcript abundance associated with the reproductive cycle and exposure to endocrine disrupting chemicals. Fish Physiology and Biochemistry, 28(1-4), 29-32. DOI:10.1023/B:FISH.0000030466.23085.94
  • Kazeto, Y., Place, A. R., & Trant, J. M. (2004). Effects of endocrine disrupting chemicals on the expression of CYP19 genes in zebrafish (Danio rerio) juveniles. Aquatic Toxicology, 69(1), 25-34. DOI: 10.1016/j.aquatox.2004.04.008
  • Kazeto, Y., & Trant, J. M. (2005). Molecular biology of channel catfish brain cytochrome P450 aromatase (CYP19A2): Cloning, preovulatory induction of gene expression, hormonal gene regulation and analysis of promoter region. Journal of Molecular Endocrinology, 35(3), 571-583. DOI:10.1677/jme.1.01805
  • Kishida, M., & Callard, G. V. (2001). Distinct cytochrome p450 Aromatase Isoforms in Zebrafish (Danio rerio) brain and ovary are differentially Programmedand estrogen regulated during early Development. Endocrinology, 142(2), 740-750. doi.org/10.1210/endo.142.2.7928
  • Kishida, M., McLellan, M., Miranda, J. A., & Callard, G. V. (2001). Estrogen and xenoestrogens upregulate the brain aromatase isoform (P450aromB) and perturb markers of early development in zebrafish (Danio rerio). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 129(2-3), 261-268. DOI:10.1016/S1096-4959(01)00319-0
  • Kwon, J. Y., McAndrew, B. J., & Penman, D. J. (2001). Cloning of brain aromatase gene and expression of brain and ovarian aromatase genes during sexual differentiation in genetic male and female Nile tilapia Oreochromis niloticus. Molecular Reproduction and Development, 59(4), 359-370. DOI:10.1002/mrd.1042
  • Lange, A., Katsu, Y., Ichikawa, R., Paull, G. C., Chidgey, L. L., Coe, T. S. & Tyler, C. R. (2008). Altered sexual development in roach (Rutilus rutilus) exposed to environmental concentrations of the pharmaceutical 17α-Ethinylestradiol and associated expression dynamics of Aromatases and estrogen receptors. Toxicological Sciences, 106(1), 113-123. DOI: 10.1093/toxsci/kfn151
  • Marttinen, S. K., Kettunen, R. H., Sormunen, K. M., & Rintala, J. A. (2003). Removal of bis(2-ethylhexyl) phthalate at a sewage treatment plant. Water Research, 37(6), 1385-1393. DOI: 10.1016/S0043-1354(02)00486-4
  • Matsuoka, M. P., Van Nes, S., Andersen, Ø., Benfey, T. J., & Reith, M. (2006). Real-time PCR analysis of ovary- and brain-type aromatase gene expression during Atlantic halibut (Hippoglossus hippoglossus) development. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 144(1), 128-135. DOI: 10.1016/j.cbpb.2006.02.008
  • Nakamura, I., Evans, J. C., Kusakabe, M., Nagahama, Y., & Young, G. (2005). Changes in steroidogenic enzyme and steroidogenic acute regulatory protein Messenger RNAs in ovarian follicles during ovarian development of rainbow trout (Oncorhynchus mykiss). General and Comparative Endocrinology, 144(3), 224-231. DOI:10.1016/j.ygcen.2005.06.004
  • Nunez, B. S., & Applebaum, S. L. (2006). Tissue- and sex-specific regulation of CYP19A1 expression in the Atlantic croaker (Micropogonias undulatus). General and Comparative Endocrinology, 149(2), 205-216. DOI:10.1016/j.ygcen.2006.06.005
  • Pasmanik, M., & Callard, G. V. (1985). Aromatase and 5α-reductase in the teleost brain, spinal cord, and pituitary gland. General and Comparative Endocrinology, 60(2), 244-251. DOI:10.1016/0016-6480(85)90320-X
  • Pfaffl, M. W. (2001). A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research, 29(9), 45e-45. DOI:10.1093/nar/29.9.e45
  • Rasheeda, M., Sridevi, P., & Senthilkumaran, B. (2010). Cytochrome P450 aromatases: Impact on gonadal development, recrudescence and effect of hCG in the catfish, Clarias gariepinus. General and Comparative Endocrinology, 167(2), 234-245. DOI:10.1016/j.ygcen.2010.03.009
  • Sawyer, S. J., Gerstner, K. A., & Callard, G. V. (2006). Real-time PCR analysis of cytochrome P450 aromatase expression in zebrafish: Gene specific tissue distribution, sex differences, developmental programming, and estrogen regulation. General and Comparative Endocrinology, 147(2), 108-117. DOI:10.1016/j.ygcen.2005.12.010
  • Simon, P. (2003). Q-gene: Processing quantitative real-time RT-PCR data. Bioinformatics, 19(11), 1439-1440. DOI:10.1093/bioinformatics/btg157
  • Tchoudakova, A., & Callard, G. V. (1998). Identification of multiple CYP19 genes encoding different cytochrome P450 Aromatase Isozymes in brain and ovary*. Endocrinology, 139(4), 2179-2189. DOI:10.1210/endo.139.4.5899
  • Tchoudakova, A., Kishida, M., Wood, E., & Callard, G. V. (2001). Promoter characteristics of two cyp19 genes differentially expressed in the brain and ovary of teleost fish. The Journal of Steroid Biochemistry and Molecular Biology, 78(5), 427-439. DOI:10.1016/S0960-0760(01)00120-0
  • Unal, G., Çetinkaya, O. and Elp, M. (1999). Histological Investigation of Gonad Development of Chalcalburnus tarichi (P. 1811). Turkish Zoology, 23, 329–338. DOI:10.1016/j.ygcen.2006.06.005
  • Unal, G., Marquez, E. C., Feld, M., Stavropoulos, P., & Callard, I. P. (2014). Isolation of estrogen receptor subtypes and vitellogenin genes: Expression in female Chalcalburnus tarichi. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 172-173, 67-73. DOI:10.1016/j.cbpb.2014.04.002
  • Ünal, G., Türkoğlu, V., Oğuz, A. R., & Kaptaner, B. (2007). Gonadal histology and some biochemical characteristics of Chalcalburnus tarichi (Pallas, 1811) having abnormal gonads. Fish Physiology and Biochemistry, 33(2), 153-165. DOI:10.1007/s10695-006-9126-1
  • Villeneuve, D.L., Knoebl, I., Kahl, M. D., Jensen, K. M., Hammermeister, D. E., Greene, K. J. & Ankley, G. T. (2006). Relationship between brain and ovary aromatase activity and isoform-specific aromatase mRNA expression in the fathead minnow (Pimephales promelas). Aquatic Toxicology, 76(3-4), 353-368. DOI:10.1016/j.aquatox.2005.10.016

Dişi Van balığı (Alburnus tarichi, Guldenstaedtii, 1814)’nda sitokrom P450 aromataz izoformlarının ekspresyonu

Year 2021, Volume: 38 Issue: 2, 247 - 252, 15.06.2021

Güler Ünal Emily Marquez Mara O'brien Pericles Stavropoulos Ian P. Callard

https://doi.org/10.12714/egejfas.38.2.15

Abstract


Bu çalışmanın amacı, Van Gölü’nden yakalanan dişi Alburnus tarichi’de P450 aromataz izoformları olan beyin kaynaklı Cyp19b ve ovaryum kaynaklı Cyp19a genlerini klonlanmak ve üreme bakımından ovaryum gelişimi engellenmiş (RA) ve ovaryum gelişimi normal olan (RN) balıklardan alınan hypothalamus ve ovaryum örneklerinde bu genlerin ekspresyon seviyelerini belirlemektir. Cyp19b gen ekspersyonu beyinde, Cyp19a gen ekspresyonu ise ovaryumda daha fazlaydı. Cyp19b mRNA seviyesi, RA balıkların ovaryum ve hipotalamusunda, RN balıkların ovaryum ve hipotalamusundan belirgin olarak düşüktü (P<0.05). CYP19a ekspresyon seviyesi, hem RN ve hem de RA balıkların hipotalamusunda fark bulunmazken RA balıkların ovaryumunda, RN balıkların ovaryumundan belirgin olarak düşüktü (P<0.05). Bu sonuçlara bize, RA balıkların populasyonun bir parçası olduğunu ve bu balıkların endokrin bozucu bileşik veya bileşiklere diğer balıklardan daha çok duyarlı olabileceğini düşündürmektedir. 

  

Keywords

P450 aromatase gen ekspresyonu Alburnus tarichi Van Gölü

Project Number

NIEHS-07381

References

  • Barney, M. L., Patil, J. G., Gunasekera, R. M., & Carter, C. G. (2008). Distinct cytochrome P450 aromatase isoforms in the common carp (Cyprinus carpio): Sexual dimorphism and onset of ontogenic expression. General and Comparative Endocrinology, 156(3), 499-508. DOI:10.1016/j.ygcen.2008.03.013
  • Callard, G. V., Tchoudakova, A. V., Kishida, M., & Wood, E. (2001). Differential tissue distribution, developmental programming, estrogen regulation and promoter characteristics of cyp19 genes in teleost fish. The Journal of Steroid Biochemistry and Molecular Biology, 79(1-5), 305-314. DOI:10.1016/S0960-0760(01)00147-9
  • Chang, X., Kobayashi, T., Senthilkumaran, B., Kobayashi-Kajura, H., Sudhakumari, C. C., & Nagahama, Y. (2005). Two types of aromatase with different encoding genes, tissue distribution and developmental expression in Nile tilapia (Oreochromis niloticus). General and Comparative Endocrinology, 141(2), 101-115. DOI:10.1016/j.ygcen.2004.11.020
  • Cheshenko, K., Brion, F., Le Page, Y., Hinfray, N., Pakdel, F., Kah, O., … Eggen, R. I. (2006). Expression of zebra fish Aromatase cyp19a and cyp19b genes in response to the ligands of estrogen receptor and aryl hydrocarbon receptor. Toxicological Sciences, 96(2), 255-267. DOI:10.1093/toxsci/kfm003
  • Cheshenko, K., Pakdel, F., Segner, H., Kah, O., & Eggen, R. I. (2008). Interference of endocrine disrupting chemicals with aromatase CYP19 expression or activity, and consequences for reproduction of teleost fish. General and Comparative Endocrinology, 155(1), 31-62. DOI:10.1016/j.ygcen.2007.03.005
  • Chiang, E. F., Yan, Y., Guiguen, Y., Postlethwait, J., & Chung, B. (2001). Two Cyp19 (P450 Aromatase) genes on duplicated Zebrafish chromosomes are expressed in ovary or brain. Molecular Biology and Evolution, 18(4), 542-550. DOI:10.1093/oxfordjournals.molbev.a003833
  • Dong, W., & Willett, K. L. (2008). Local expression of CYP19A1 and CYP19A2 in developing and adult killifish (Fundulus heteroclitus). General and Comparative Endocrinology, 155(2), 307-317. DOI:10.1016/j.ygcen.2007.05.018
  • EPA (2001). Sampling procedures for the 2001 National Sewage Sludge Survey. Office of Science and Technology Washington, D.C. www.epa.gov.
  • Fenske, M., & Segner, H. (2004). Aromatase modulation alters gonadal differentiation in developing zebrafish (Danio rerio). Aquatic Toxicology, 67(2), 105-126. DOI: 10.1016/j.aquatox.2003.10.008
  • Forlano, P. M., Deitcher, D. L., Myers, D. A., & Bass, A. H. (2001). Anatomical distribution and cellular basis for high levels of Aromatase activity in the brain of teleost fish: Aromatase enzyme and mRNA expression identify glia as source. The Journal of Neuroscience, 21(22), 8943-8955. DOI:10.1523/JNEUROSCI.21-22-08943.2001
  • Gelinas, D., A. Pitoc, G., & V. Callard, G. (1998). Isolation of a goldfish brain cytochrome P450 aromatase cDNA:. Molecular and Cellular Endocrinology, 138(1-2), 81-93. DOI: 10.1016/S0303-7207(98)00015-X
  • Greytak, S. R., Champlin, D., & Callard, G. V. (2005). Isolation and characterization of two cytochrome P450 aromatase forms in killifish (Fundulus heteroclitus): Differential expression in fish from polluted and unpolluted environments. Aquatic Toxicology, 71(4), 371-389. DOI:10.1016/j.aquatox.2004.12.007
  • Kazeto, Y., Goto-Kazeto, R., Place, A., & Trant, J. (2003). Aromatase expression in zebrafish and channel catfish brains: Changes in transcript abundance associated with the reproductive cycle and exposure to endocrine disrupting chemicals. Fish Physiology and Biochemistry, 28(1-4), 29-32. DOI:10.1023/B:FISH.0000030466.23085.94
  • Kazeto, Y., Place, A. R., & Trant, J. M. (2004). Effects of endocrine disrupting chemicals on the expression of CYP19 genes in zebrafish (Danio rerio) juveniles. Aquatic Toxicology, 69(1), 25-34. DOI: 10.1016/j.aquatox.2004.04.008
  • Kazeto, Y., & Trant, J. M. (2005). Molecular biology of channel catfish brain cytochrome P450 aromatase (CYP19A2): Cloning, preovulatory induction of gene expression, hormonal gene regulation and analysis of promoter region. Journal of Molecular Endocrinology, 35(3), 571-583. DOI:10.1677/jme.1.01805
  • Kishida, M., & Callard, G. V. (2001). Distinct cytochrome p450 Aromatase Isoforms in Zebrafish (Danio rerio) brain and ovary are differentially Programmedand estrogen regulated during early Development. Endocrinology, 142(2), 740-750. doi.org/10.1210/endo.142.2.7928
  • Kishida, M., McLellan, M., Miranda, J. A., & Callard, G. V. (2001). Estrogen and xenoestrogens upregulate the brain aromatase isoform (P450aromB) and perturb markers of early development in zebrafish (Danio rerio). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 129(2-3), 261-268. DOI:10.1016/S1096-4959(01)00319-0
  • Kwon, J. Y., McAndrew, B. J., & Penman, D. J. (2001). Cloning of brain aromatase gene and expression of brain and ovarian aromatase genes during sexual differentiation in genetic male and female Nile tilapia Oreochromis niloticus. Molecular Reproduction and Development, 59(4), 359-370. DOI:10.1002/mrd.1042
  • Lange, A., Katsu, Y., Ichikawa, R., Paull, G. C., Chidgey, L. L., Coe, T. S. & Tyler, C. R. (2008). Altered sexual development in roach (Rutilus rutilus) exposed to environmental concentrations of the pharmaceutical 17α-Ethinylestradiol and associated expression dynamics of Aromatases and estrogen receptors. Toxicological Sciences, 106(1), 113-123. DOI: 10.1093/toxsci/kfn151
  • Marttinen, S. K., Kettunen, R. H., Sormunen, K. M., & Rintala, J. A. (2003). Removal of bis(2-ethylhexyl) phthalate at a sewage treatment plant. Water Research, 37(6), 1385-1393. DOI: 10.1016/S0043-1354(02)00486-4
  • Matsuoka, M. P., Van Nes, S., Andersen, Ø., Benfey, T. J., & Reith, M. (2006). Real-time PCR analysis of ovary- and brain-type aromatase gene expression during Atlantic halibut (Hippoglossus hippoglossus) development. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 144(1), 128-135. DOI: 10.1016/j.cbpb.2006.02.008
  • Nakamura, I., Evans, J. C., Kusakabe, M., Nagahama, Y., & Young, G. (2005). Changes in steroidogenic enzyme and steroidogenic acute regulatory protein Messenger RNAs in ovarian follicles during ovarian development of rainbow trout (Oncorhynchus mykiss). General and Comparative Endocrinology, 144(3), 224-231. DOI:10.1016/j.ygcen.2005.06.004
  • Nunez, B. S., & Applebaum, S. L. (2006). Tissue- and sex-specific regulation of CYP19A1 expression in the Atlantic croaker (Micropogonias undulatus). General and Comparative Endocrinology, 149(2), 205-216. DOI:10.1016/j.ygcen.2006.06.005
  • Pasmanik, M., & Callard, G. V. (1985). Aromatase and 5α-reductase in the teleost brain, spinal cord, and pituitary gland. General and Comparative Endocrinology, 60(2), 244-251. DOI:10.1016/0016-6480(85)90320-X
  • Pfaffl, M. W. (2001). A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research, 29(9), 45e-45. DOI:10.1093/nar/29.9.e45
  • Rasheeda, M., Sridevi, P., & Senthilkumaran, B. (2010). Cytochrome P450 aromatases: Impact on gonadal development, recrudescence and effect of hCG in the catfish, Clarias gariepinus. General and Comparative Endocrinology, 167(2), 234-245. DOI:10.1016/j.ygcen.2010.03.009
  • Sawyer, S. J., Gerstner, K. A., & Callard, G. V. (2006). Real-time PCR analysis of cytochrome P450 aromatase expression in zebrafish: Gene specific tissue distribution, sex differences, developmental programming, and estrogen regulation. General and Comparative Endocrinology, 147(2), 108-117. DOI:10.1016/j.ygcen.2005.12.010
  • Simon, P. (2003). Q-gene: Processing quantitative real-time RT-PCR data. Bioinformatics, 19(11), 1439-1440. DOI:10.1093/bioinformatics/btg157
  • Tchoudakova, A., & Callard, G. V. (1998). Identification of multiple CYP19 genes encoding different cytochrome P450 Aromatase Isozymes in brain and ovary*. Endocrinology, 139(4), 2179-2189. DOI:10.1210/endo.139.4.5899
  • Tchoudakova, A., Kishida, M., Wood, E., & Callard, G. V. (2001). Promoter characteristics of two cyp19 genes differentially expressed in the brain and ovary of teleost fish. The Journal of Steroid Biochemistry and Molecular Biology, 78(5), 427-439. DOI:10.1016/S0960-0760(01)00120-0
  • Unal, G., Çetinkaya, O. and Elp, M. (1999). Histological Investigation of Gonad Development of Chalcalburnus tarichi (P. 1811). Turkish Zoology, 23, 329–338. DOI:10.1016/j.ygcen.2006.06.005
  • Unal, G., Marquez, E. C., Feld, M., Stavropoulos, P., & Callard, I. P. (2014). Isolation of estrogen receptor subtypes and vitellogenin genes: Expression in female Chalcalburnus tarichi. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 172-173, 67-73. DOI:10.1016/j.cbpb.2014.04.002
  • Ünal, G., Türkoğlu, V., Oğuz, A. R., & Kaptaner, B. (2007). Gonadal histology and some biochemical characteristics of Chalcalburnus tarichi (Pallas, 1811) having abnormal gonads. Fish Physiology and Biochemistry, 33(2), 153-165. DOI:10.1007/s10695-006-9126-1
  • Villeneuve, D.L., Knoebl, I., Kahl, M. D., Jensen, K. M., Hammermeister, D. E., Greene, K. J. & Ankley, G. T. (2006). Relationship between brain and ovary aromatase activity and isoform-specific aromatase mRNA expression in the fathead minnow (Pimephales promelas). Aquatic Toxicology, 76(3-4), 353-368. DOI:10.1016/j.aquatox.2005.10.016
There are 34 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Articles
Authors

Güler Ünal 0000-0003-1035-4809

Emily Marquez 0000-0003-1391-4664

Mara O'brien 0000-0002-5646-2541

Pericles Stavropoulos 0000-0003-0985-6203

Ian P. Callard

Project Number NIEHS-07381
Publication Date June 15, 2021
Submission Date January 14, 2021
Published in Issue Year 2021Volume: 38 Issue: 2

Cite

APA Ünal, G., Marquez, E., O’brien, M., Stavropoulos, P., et al. (2021). Expression of cytochrome P450 aromatase isoforms in female Alburnus tarichi (Guldenstaedtii, 1814). Ege Journal of Fisheries and Aquatic Sciences, 38(2), 247-252. https://doi.org/10.12714/egejfas.38.2.15