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Localization of the CCK-8, Gastrin 1, VIP, Sekretin, Somatostatin-14, Bombesin and Histamin peptids in Gastrointestinal Tract Mucous Membrane of Common Carp (Cyprinus carpio)

Year 2009, Volume: 26 Issue: 1, 11 - 17, 01.03.2009

Nurgül Şenol Kenan Çınar Ülker Eren

Abstract

In this study, it was aimed to identify the distribution of cholecystokinin-8 (CCK-8), gastrin 1, vasoactive intestinal polypeptide (VIP), secretin, somatostatin-14, bombesin, and histamine peptides within the gastrointestinal tract mucosa of carp (Cyprinus carpio). As a result of the immunohistochemical studies, all the peptides studied were determined to be localized generally in different distribution within the stomachs of carp (Cyprinus carpio) (the enlarged area of anterior intestine) as well as within their intestines (anterior, middle and posterior intestine). 

Keywords

Common Carp Gastrointestinal Tract Peptide Cyprinus carpio

References

  • Abad, M.E., F. M. Binkhorst, M.T. Elbal, and J.H. Rombout. 1987. A comparative immunocytochemical study of the gastro-entero-pancreatic (gep) endocrine system in a stomachless and a stomach-containing teleost. General and Comparative Endocrinology, 66(1): 123–36.
  • Alagöz, S., 2005. Seyhan baraj gölü (Adana) balık faunasının belirlenmesi. Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, 82s, Adana.
  • Al-Mahrouki, A.A. and J.H. Youson. 1998. Immunohistochemical studies of the endocrine cells within the gastro-entero-pancreatic system of osteoglossomorpha, an ancient teleostean group. General and Comparative Endocrinology, 110(2): 125–39.
  • Balon, E.K. 1995. Origin and domestication of the wild carp, Cyprinus carpio:from roman gourmets to the swimming flowers. Aquaculture, 129, 3–48.
  • Barrenechea, M.A., J. Lopez, and A. Martinez. 1994. Regulatory peptides in gastric endocrine cells of the rainbow trout (Oncorhynchus mykiss) general distribution and colocalizations. Tissue and Cell, 26(3): 309–321.
  • Boerlegui, C., A. Martinez, P. Sesma. 1992. Endocrine cells and nerves in the pyloric caeca and ıntestine of Oncorhynchus mykiss (Teleostei): an immunocytochemical study. General and Comparative Endocrinology, 86: 483-95.
  • Bomgren, P., S. Einarsson, and A.C. Jonsson. 1998. Similarities and differences in oxyticopeptic cell ultrastructure of one marine teleost, Gadus morhua and one freshwater teleost, Oncorhynchus mykiss, during basal and histamine stimulated phases of acid secretion. Fish Physiology and Biochemistry, 18: 285–296.
  • Bosi, G., A.P. Shinn, L. Giari, F. Simoni, and B.S. Dezfuli. 2005. Changes in the neuromodulators of the diffuse endocrine system of the alimentary canal of farmed rainbow trout, Oncorhynchus mykiss (Walbaum), naturally infected with Eubothrium crassum (Cestoda). Journal of Fish Diseases, 28: 703–711.
  • Bozkurt, Y., S. Seçer, 2006. Aynalı Sazan (Cyprinus Carpio) Balıklarında Üreme Mevsimi Boyunca Spermatolojik Özelliklerin Belirlenmesi. Ege Üniversitesi Su Ürünleri Dergisi, 23, 195–198.
  • Cahu, C., I. Ronnestad, V. Grangier, and J.L.Z. Infante. 2004. Expression and activities of pancreatic enzymes in developing sea bass larva (dicentrarchus labrax) in relation to intact and hydrolyzed dietary protein: involvement of cholecystokinin. Aquaculture, 2–36.
  • Cimini, V., S. Noorder, V. Nardini. 1989. Peptides of the Gastrointestinal Tract of the dogfish (Scyliorhinus stellaris). 8th Int. Symp. On Morphological Sciences, 146-57.
  • Çelikkale, M.S. 1991. Balık Biyolojisi. Karadeniz Teknik Üniversitesi Sürmene Deniz Bilimleri ve Teknolojisi Yüksekokulu Yayınları, No:101, Trabzon, 387s.
  • Dezfuli, B.S., S. Arrighi, C. Domeneghini, and G. Bosi. 2000. Immunohistochemical detection of neuromodulators in the intestine of Salmo trutta l. naturally infected with Cyathocephalus truncatus Pallas (Cestoda). Journal of Fish Diseases, 23: 265–273.
  • Dezfuli, B.S., F. Pironi, L. Giari, C. Domeneghini, G. Bosi. 2002. Effect of Pomphorynchus laevis (Acanthocephala) on putative neuromodulator in the intestine of naturally infected Salmo trutta. Diseases of Aquatic Organs, 51: 27–35.
  • Dezfuli, B.S., L. Giari, S. Arrighi, C. Domeneghini, and G. Bosi. 2003. Influence of enteric helminths on the distribution of intestinal endocrine cells belonging to the diffuse endocrine system in brown trout, Salmo trutta L. Journal of Fih Diseases, 26: 155-166.
  • Domeneghini, C., G. Radaelli, S. Arrighi, F. Mascarello, and A. Veggetti. 2000. Neurotransmitters and putative neuromodulators in the gut of Anguilla anguilla (l.). localizations in the enteric nervous and endocrine systems. European Journal of Histochemistry, 44(3): 295–306.
  • Elbal, M.T. and M.T. Agulleiro. 1986. An immunocytochemical and ultrastructural study of endocrine cells in the gut of a teleost fish, Sparus auratus L. General and Comparative Endocrinology, 64(3): 339-54.
  • Ellis, A.E. 1985. Eosinophilic Granular Cells (EGC) and histamine responses to toxins in rainbow trout. Developmental and Comparative Immunology, 9(2): 251–260.
  • Fairgrieve, W.T., M.S. Myers, R.W. Hardy, and F.M. Dong, 1994. Gastric abnormalities in rainbow trout (Oncorhynchus mykiss) fed amine supplemented diets or chicken gizzard erosion positive fish meal. Aquaculture, 127(3): 219–232.
  • Girolamo, P., C. Lucini, J.A. Vega, G. Andreozzi, L. Coppola, and L. Castaldo. 1999. Co-Localization of trk neurotrophin receptors and regulatory peptides in the endocrine cells of the teleostean stomach. The Anatomical Record, 256: 219–226.
  • Gökoğlu, N. 2002. Su Ürünleri İşleme Teknolojisi. Su Vakfı Yayınları, İstanbul, 157s.
  • Himick, B.A. and R.E. Peter. 1994. CCK/Gastrin like immunreactivity in brain and gut, and cck suppression of feeding in gold fish. Intergrative and Comparative Physiology, 267(3): 841–851.
  • Holmgren, S., S. Nilsson. 1983. Bombesin-gastrin/ CCK-5-Hydroxitryptamine- Neurotensin-somatostatin and VIP-like immunreactivity and catecholamine fluorescense in the gut of Elasmobranch, Squalus acanthias. Cell Tissue Research: 234, 595–618.
  • Holm, P.B., S. Holmgren. 1989. A comparative stuy of neuropeptides in the intestine of two stomachless teleost (Poecilioa reticulata, Leuciscus idus melanatus) under conditions of feding and starvation. Cell Tissue Research, 255: 245–254.
  • Holmgren, S., A.C. Jonsson, B. Holstein. 1986. Gastrointestinal peptides in fish. In: Nilssonn, S., Holmgren, S., (eds) Fish Phsiyology: Recent advances Crom Helm., NewHampshire, pp.119-39.
  • Jensen, J., S. Holmgren, A. Johnson. 1987. Substance-P like immunreactivity and the effects of tachykinins in the intestine of the Atlantic cod Gadus morhua. Journal Autonom Nervous System, 20: 25-30.
  • Jonsson, A.C., S. Holmgren, B. Holstein. 1987. Gastrin/CCK-like immunoreactivity in endocrine cells and nerves in the gastrointestinal tract of cod, Gadus morhua and the effect of peptides of gastrin/cck family on cod gastrointestinal smooth muscle. General and Comparative Endocrinology, 66: 190–202.
  • Kiliaan, A.J., G. Scholten, and J.A. Graot. 1997. Exocytotic release of vasoactive ıntestinal polypeptide and serotonin from mucosal nerve fibres and endocrine and the tilapia (Oreochromis mossambicus): an ultrastructural study. Histochemical Journal, 29: 45–51.
  • Kim, J.B., V. Gadsboll, J. Whittaker, B.A. Barton, and J.M. Conlon. 2000. Gastroenteropancreatic hormones (insulin, glucagon, somatostatin, and multiple forms of pyy) from the pallid sturgeor, Scarphirhynchus albus (Acipenseriformes). General and Comparative Endocrinology, 120(3): 353–363.
  • Ku, S.K., J.H. Lee, and H.S. Lee. 2004. Immunohistochemical study on the endocrine cells in the gut of the stomachless teleost, Zacco platypus (Cyprinidae). Anatomia Histologia Embryologia, 33: 212–219.
  • Lee, J.H., S.K. Ku, K.D. Park, and H.S. Lee. 2004. Immunohistochemical study of the gastrointestinal endocrine cells in the Korean aucha perch. Journal of Fish Biology, 65: 170–181.
  • Linhart, O., S. Kudo, R. Billard, V. Slechta, and E.V. Mikodina. 1995. Morphology composition and fertilization of carp eggs: a review. Aquaculture, 129: 75–93.
  • Maake, C., C. Kaufmann, and M. Reinecke. 2001. Ontogeny of neurohormonal peptides, serotonin and nitric oxide synthase in the gastrointestinal neuroendocrine system of the axolotl (Ambystoma mexicanum): an immunohistochemical analysis.. General and Comparative Endocrinology, 121(1): 74–83.
  • Paleologos, E.K., I.N. Savvaidis, and M.G. Kontomineas. 2004. Biogenic amines formation and its relation to microbiological and sensory attributes in ice-stored whole gutted and filleted mediterranean sea bass (Dicentrarchus labrax). Food Microbiology, 21(5): 549–557.
  • Pan, Q.S., Z.P. Fang, and F.J. Huang 2000a. Identification, localization and morphology of APUD cells in gastroenteropancreatic system of stomach- containing teleosts. World Journal of Gastroenterology, 6(6): 842–847.
  • Pan, Q.S., Z.P. Fang and Y.X. Zhao. 2000b. Immunocytochemical identification and localization of APUD cells in the gut of seven stomachless teleost fishes. World Journal of Gastroenterology, 6(1): 96– 101.
  • Rajjo, I.M., S.R. Vigna, and J.W. Crim,. 1988. Cholecystokinin immunreactivity in the digestive tract of bowfin (Amia calva), bluegill (Lepomis macrochirus) and bulfrog (Rana Catesbeina). General and Comparative Endocrinology, 70: 133–44.
  • Rajjo, I.M., S.R. Vigna, and J.W. Crim. 1989. Immunohistochemical localization of bombesin-like peptides in the digestive tract of the bowfin, Amia calva. Comparative and Biochemistry Phsiology, 94(2): 405–409.
  • Reite, O.B. 1997. Mast cells eosinophilic granule cells of salmonids staining properties and response to noxious agents. Fish and Shellfish Immunology, 7(8): 567–584.
  • Rombout, J.H. and M. Reinecke. 1984. Immunohistochemical localization of (neuro) peptide hormones in endocrine cells and nerves of the gut of a stomachless teleost fish, Barbus conchonius (Cyprinidae). Cell Tissue Research, 237(1): 57–65.
  • Scheuermann, D.W., D. Adriaensen, J.P. Timmermans, and M.H. De Groodt- Lasseel 1991. immunohistochemical localization of polypeptide hormones in pancreatic endocrine cells of a Dipnoan Fish, Protopterus aethiopicus. Acta Histochemistry, 9(2): 185–92.
  • Sternberger, L.A. 1979. The unlabeled antibody peroxidase-antiperoxidase (PAP) method. In immunocytochemistry (Sternberger, L. A., ed.). New York, pp.104-169.
  • Vigna, S.R., B.L. Fischer, J.L.M. Morgan, G.L. Rosenquist. 1985. Distribution and molecular heterogenity of cholecystokinin like immunreactive peptides in the brain and gut of the rainbow trout, Salmo gairdneri. Comparative Biochemistry Physiology: 82, 143–46.
  • Wang, J., H. Lui, H. Po, L. Fan. 1997. Influence of salinity on food consumption, growth and energy conversion efficiency of common carp (Cyprinus carpio) fingerlings. Aquaculture, 148: 115–124.
  • Youson, J.H., A.A. Al-Mahrouki, D. Naumovski, and J.M. Conlon, 2001. The Endocrine Cells in the Gastroenteropancreatic System of the Bowfin, Amia calva L: An Immunohistochemical, ultrastructural and Immunohistochemical analysis. Journal of Morphology, 250(3): 208–24.

Sazan (Cyprinus carpio) Balığı Gastrointestinal Kanal Mukozasındaki CCK8, Gastrin 1, VIP, Sekretin, Somatostatin-14, Bombesin ve Histamin Peptidlerinin Lokalizasyonu

Year 2009, Volume: 26 Issue: 1, 11 - 17, 01.03.2009

Nurgül Şenol Kenan Çınar Ülker Eren

Abstract

Bu çalışmada; Cyprinus carpio (sazan) gastrointestinal kanal mukozasının kolesistokinin-8, gastrin 1, vasoaktif intesinal polypeptid, sekretin, somatostatin-14, bombesin ve histamin peptidlerinin lokalizasyon ve dağılımlarının belirlenmesi amaçlanmıştır. Yapılan immunohistokimyasal çalışmalar sonucunda sazan (Cyprinus carpio)’ın mide (sazanda genişlemiş ön bağırsak bölgesi) ve bağırsak bölümlerinde (ilk, orta ve son bağırsak) genel olarak çalışılan tüm peptidlerin farklı yoğunlukta lokalize oldukları tespit edildi

Keywords

Sazan Gastrointestinal Kanal Cyprinus carpio

References

  • Abad, M.E., F. M. Binkhorst, M.T. Elbal, and J.H. Rombout. 1987. A comparative immunocytochemical study of the gastro-entero-pancreatic (gep) endocrine system in a stomachless and a stomach-containing teleost. General and Comparative Endocrinology, 66(1): 123–36.
  • Alagöz, S., 2005. Seyhan baraj gölü (Adana) balık faunasının belirlenmesi. Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, 82s, Adana.
  • Al-Mahrouki, A.A. and J.H. Youson. 1998. Immunohistochemical studies of the endocrine cells within the gastro-entero-pancreatic system of osteoglossomorpha, an ancient teleostean group. General and Comparative Endocrinology, 110(2): 125–39.
  • Balon, E.K. 1995. Origin and domestication of the wild carp, Cyprinus carpio:from roman gourmets to the swimming flowers. Aquaculture, 129, 3–48.
  • Barrenechea, M.A., J. Lopez, and A. Martinez. 1994. Regulatory peptides in gastric endocrine cells of the rainbow trout (Oncorhynchus mykiss) general distribution and colocalizations. Tissue and Cell, 26(3): 309–321.
  • Boerlegui, C., A. Martinez, P. Sesma. 1992. Endocrine cells and nerves in the pyloric caeca and ıntestine of Oncorhynchus mykiss (Teleostei): an immunocytochemical study. General and Comparative Endocrinology, 86: 483-95.
  • Bomgren, P., S. Einarsson, and A.C. Jonsson. 1998. Similarities and differences in oxyticopeptic cell ultrastructure of one marine teleost, Gadus morhua and one freshwater teleost, Oncorhynchus mykiss, during basal and histamine stimulated phases of acid secretion. Fish Physiology and Biochemistry, 18: 285–296.
  • Bosi, G., A.P. Shinn, L. Giari, F. Simoni, and B.S. Dezfuli. 2005. Changes in the neuromodulators of the diffuse endocrine system of the alimentary canal of farmed rainbow trout, Oncorhynchus mykiss (Walbaum), naturally infected with Eubothrium crassum (Cestoda). Journal of Fish Diseases, 28: 703–711.
  • Bozkurt, Y., S. Seçer, 2006. Aynalı Sazan (Cyprinus Carpio) Balıklarında Üreme Mevsimi Boyunca Spermatolojik Özelliklerin Belirlenmesi. Ege Üniversitesi Su Ürünleri Dergisi, 23, 195–198.
  • Cahu, C., I. Ronnestad, V. Grangier, and J.L.Z. Infante. 2004. Expression and activities of pancreatic enzymes in developing sea bass larva (dicentrarchus labrax) in relation to intact and hydrolyzed dietary protein: involvement of cholecystokinin. Aquaculture, 2–36.
  • Cimini, V., S. Noorder, V. Nardini. 1989. Peptides of the Gastrointestinal Tract of the dogfish (Scyliorhinus stellaris). 8th Int. Symp. On Morphological Sciences, 146-57.
  • Çelikkale, M.S. 1991. Balık Biyolojisi. Karadeniz Teknik Üniversitesi Sürmene Deniz Bilimleri ve Teknolojisi Yüksekokulu Yayınları, No:101, Trabzon, 387s.
  • Dezfuli, B.S., S. Arrighi, C. Domeneghini, and G. Bosi. 2000. Immunohistochemical detection of neuromodulators in the intestine of Salmo trutta l. naturally infected with Cyathocephalus truncatus Pallas (Cestoda). Journal of Fish Diseases, 23: 265–273.
  • Dezfuli, B.S., F. Pironi, L. Giari, C. Domeneghini, G. Bosi. 2002. Effect of Pomphorynchus laevis (Acanthocephala) on putative neuromodulator in the intestine of naturally infected Salmo trutta. Diseases of Aquatic Organs, 51: 27–35.
  • Dezfuli, B.S., L. Giari, S. Arrighi, C. Domeneghini, and G. Bosi. 2003. Influence of enteric helminths on the distribution of intestinal endocrine cells belonging to the diffuse endocrine system in brown trout, Salmo trutta L. Journal of Fih Diseases, 26: 155-166.
  • Domeneghini, C., G. Radaelli, S. Arrighi, F. Mascarello, and A. Veggetti. 2000. Neurotransmitters and putative neuromodulators in the gut of Anguilla anguilla (l.). localizations in the enteric nervous and endocrine systems. European Journal of Histochemistry, 44(3): 295–306.
  • Elbal, M.T. and M.T. Agulleiro. 1986. An immunocytochemical and ultrastructural study of endocrine cells in the gut of a teleost fish, Sparus auratus L. General and Comparative Endocrinology, 64(3): 339-54.
  • Ellis, A.E. 1985. Eosinophilic Granular Cells (EGC) and histamine responses to toxins in rainbow trout. Developmental and Comparative Immunology, 9(2): 251–260.
  • Fairgrieve, W.T., M.S. Myers, R.W. Hardy, and F.M. Dong, 1994. Gastric abnormalities in rainbow trout (Oncorhynchus mykiss) fed amine supplemented diets or chicken gizzard erosion positive fish meal. Aquaculture, 127(3): 219–232.
  • Girolamo, P., C. Lucini, J.A. Vega, G. Andreozzi, L. Coppola, and L. Castaldo. 1999. Co-Localization of trk neurotrophin receptors and regulatory peptides in the endocrine cells of the teleostean stomach. The Anatomical Record, 256: 219–226.
  • Gökoğlu, N. 2002. Su Ürünleri İşleme Teknolojisi. Su Vakfı Yayınları, İstanbul, 157s.
  • Himick, B.A. and R.E. Peter. 1994. CCK/Gastrin like immunreactivity in brain and gut, and cck suppression of feeding in gold fish. Intergrative and Comparative Physiology, 267(3): 841–851.
  • Holmgren, S., S. Nilsson. 1983. Bombesin-gastrin/ CCK-5-Hydroxitryptamine- Neurotensin-somatostatin and VIP-like immunreactivity and catecholamine fluorescense in the gut of Elasmobranch, Squalus acanthias. Cell Tissue Research: 234, 595–618.
  • Holm, P.B., S. Holmgren. 1989. A comparative stuy of neuropeptides in the intestine of two stomachless teleost (Poecilioa reticulata, Leuciscus idus melanatus) under conditions of feding and starvation. Cell Tissue Research, 255: 245–254.
  • Holmgren, S., A.C. Jonsson, B. Holstein. 1986. Gastrointestinal peptides in fish. In: Nilssonn, S., Holmgren, S., (eds) Fish Phsiyology: Recent advances Crom Helm., NewHampshire, pp.119-39.
  • Jensen, J., S. Holmgren, A. Johnson. 1987. Substance-P like immunreactivity and the effects of tachykinins in the intestine of the Atlantic cod Gadus morhua. Journal Autonom Nervous System, 20: 25-30.
  • Jonsson, A.C., S. Holmgren, B. Holstein. 1987. Gastrin/CCK-like immunoreactivity in endocrine cells and nerves in the gastrointestinal tract of cod, Gadus morhua and the effect of peptides of gastrin/cck family on cod gastrointestinal smooth muscle. General and Comparative Endocrinology, 66: 190–202.
  • Kiliaan, A.J., G. Scholten, and J.A. Graot. 1997. Exocytotic release of vasoactive ıntestinal polypeptide and serotonin from mucosal nerve fibres and endocrine and the tilapia (Oreochromis mossambicus): an ultrastructural study. Histochemical Journal, 29: 45–51.
  • Kim, J.B., V. Gadsboll, J. Whittaker, B.A. Barton, and J.M. Conlon. 2000. Gastroenteropancreatic hormones (insulin, glucagon, somatostatin, and multiple forms of pyy) from the pallid sturgeor, Scarphirhynchus albus (Acipenseriformes). General and Comparative Endocrinology, 120(3): 353–363.
  • Ku, S.K., J.H. Lee, and H.S. Lee. 2004. Immunohistochemical study on the endocrine cells in the gut of the stomachless teleost, Zacco platypus (Cyprinidae). Anatomia Histologia Embryologia, 33: 212–219.
  • Lee, J.H., S.K. Ku, K.D. Park, and H.S. Lee. 2004. Immunohistochemical study of the gastrointestinal endocrine cells in the Korean aucha perch. Journal of Fish Biology, 65: 170–181.
  • Linhart, O., S. Kudo, R. Billard, V. Slechta, and E.V. Mikodina. 1995. Morphology composition and fertilization of carp eggs: a review. Aquaculture, 129: 75–93.
  • Maake, C., C. Kaufmann, and M. Reinecke. 2001. Ontogeny of neurohormonal peptides, serotonin and nitric oxide synthase in the gastrointestinal neuroendocrine system of the axolotl (Ambystoma mexicanum): an immunohistochemical analysis.. General and Comparative Endocrinology, 121(1): 74–83.
  • Paleologos, E.K., I.N. Savvaidis, and M.G. Kontomineas. 2004. Biogenic amines formation and its relation to microbiological and sensory attributes in ice-stored whole gutted and filleted mediterranean sea bass (Dicentrarchus labrax). Food Microbiology, 21(5): 549–557.
  • Pan, Q.S., Z.P. Fang, and F.J. Huang 2000a. Identification, localization and morphology of APUD cells in gastroenteropancreatic system of stomach- containing teleosts. World Journal of Gastroenterology, 6(6): 842–847.
  • Pan, Q.S., Z.P. Fang and Y.X. Zhao. 2000b. Immunocytochemical identification and localization of APUD cells in the gut of seven stomachless teleost fishes. World Journal of Gastroenterology, 6(1): 96– 101.
  • Rajjo, I.M., S.R. Vigna, and J.W. Crim,. 1988. Cholecystokinin immunreactivity in the digestive tract of bowfin (Amia calva), bluegill (Lepomis macrochirus) and bulfrog (Rana Catesbeina). General and Comparative Endocrinology, 70: 133–44.
  • Rajjo, I.M., S.R. Vigna, and J.W. Crim. 1989. Immunohistochemical localization of bombesin-like peptides in the digestive tract of the bowfin, Amia calva. Comparative and Biochemistry Phsiology, 94(2): 405–409.
  • Reite, O.B. 1997. Mast cells eosinophilic granule cells of salmonids staining properties and response to noxious agents. Fish and Shellfish Immunology, 7(8): 567–584.
  • Rombout, J.H. and M. Reinecke. 1984. Immunohistochemical localization of (neuro) peptide hormones in endocrine cells and nerves of the gut of a stomachless teleost fish, Barbus conchonius (Cyprinidae). Cell Tissue Research, 237(1): 57–65.
  • Scheuermann, D.W., D. Adriaensen, J.P. Timmermans, and M.H. De Groodt- Lasseel 1991. immunohistochemical localization of polypeptide hormones in pancreatic endocrine cells of a Dipnoan Fish, Protopterus aethiopicus. Acta Histochemistry, 9(2): 185–92.
  • Sternberger, L.A. 1979. The unlabeled antibody peroxidase-antiperoxidase (PAP) method. In immunocytochemistry (Sternberger, L. A., ed.). New York, pp.104-169.
  • Vigna, S.R., B.L. Fischer, J.L.M. Morgan, G.L. Rosenquist. 1985. Distribution and molecular heterogenity of cholecystokinin like immunreactive peptides in the brain and gut of the rainbow trout, Salmo gairdneri. Comparative Biochemistry Physiology: 82, 143–46.
  • Wang, J., H. Lui, H. Po, L. Fan. 1997. Influence of salinity on food consumption, growth and energy conversion efficiency of common carp (Cyprinus carpio) fingerlings. Aquaculture, 148: 115–124.
  • Youson, J.H., A.A. Al-Mahrouki, D. Naumovski, and J.M. Conlon, 2001. The Endocrine Cells in the Gastroenteropancreatic System of the Bowfin, Amia calva L: An Immunohistochemical, ultrastructural and Immunohistochemical analysis. Journal of Morphology, 250(3): 208–24.
There are 45 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Nurgül Şenol

Kenan Çınar

Ülker Eren

Publication Date March 1, 2009
Submission Date December 7, 2015
Published in Issue Year 2009Volume: 26 Issue: 1

Cite

APA Şenol, N. ., Çınar, K. ., & Eren, Ü. . (2009). Sazan (Cyprinus carpio) Balığı Gastrointestinal Kanal Mukozasındaki CCK8, Gastrin 1, VIP, Sekretin, Somatostatin-14, Bombesin ve Histamin Peptidlerinin Lokalizasyonu. Ege Journal of Fisheries and Aquatic Sciences, 26(1), 11-17.