The effect of mercury, copper, and zinc on paraoxonase (PON) enzyme activity in Bonito (Sarda sarda) fish

Büşra Şahin; Sena Çenesiz

doi:10.12714/egejfas.38.4.09

EN TR

The effect of mercury, copper, and zinc on paraoxonase (PON) enzyme activity in Bonito (Sarda sarda) fish

Abstract

Since heavy metal dirtiness, which we frequently encounter in environmental pollution causes harmful effects on the organism through biochemical enzyme reactions, in this study, the effects of mercury (Hg⁺²), copper (Cu⁺²), and zinc (Zn⁺²) heavy metal ions, which are common in environmental pollution, on PON (paraoxonase) enzyme activity in muscle tissue of bonito (Sarda sarda) were investigated. In the study, 25 bonito (S. sarda) fish muscle tissues freshly obtained from the Samsun region sea were used. The changes in PON enzyme activity were determined by adding different volumes of heavy metal solutions. PON enzyme activities of Hg+2 heavy metal ion used in different volumes were calculated as 30.9383 U/mLdak, 29.0598 U/mLdak, 26.3799 U/mLdak, 23.9443 U/mLdak, 20.6725 U/mLdak, PON enzyme activities of Cu⁺² heavy metal ion used in different volumes were calculated as 19.7949 U/mLdak, 19.4807 U/mLdak 19.1864 U/mLdak, 19.1200 U/mLdak, 18.9037 U/mLdak and PON enzyme activities of Zn⁺² heavy metal ion used in different volumes were calculated as 23.8305 U/mLdak, 23.0781 U/mLdak, 22.9073 U/mLdak, 22.4324 U/mLdak, 21.8159 U/mLdak. As a result of these obtained data, activity (%) values were calculated and activity (%) graphs were drawn. As a result of the study, it was determined that increasing concentrations of Cu⁺² and Zn⁺² heavy metal ions caused a decrease in PON enzyme activity, but there was no statistically significant difference between the different concentrations used. It was determined that increasing concentrations of Hg⁺² heavy metal ion inhibited the PON enzyme activity, caused a statistically significant decrease between the activities depending on the different concentrations used (p < 0.05).

Keywords

Palamut (Sarda sarda) balığında paraoksonaz (PON) enzim aktivitesi üzerine cıva, bakır ve çinkonun etkisi

Öz

Çevre kirliliğinde sık rastlanan ağır metal kirlilikleri, biyokimyasal enzim reaksiyonları üzerinden organizmada zararlı etkiler meydana getirdiği için bu çalışmada çevre kirliliğinde sık rastlanan cıva (Hg⁺²), bakır (Cu⁺²) ve çinko (Zn⁺²) ağır metal iyonlarının palamut (Sarda sarda) balığı kas dokusunda bulunan PON (paraoksonaz) aktivitesi üzerine etkileri araştırılmıştır. Çalışmada Samsun bölgesi denizinden taze olarak temin edilen 25 adet palamut balığının kas dokuları kullanıldı. Ağır metal çözeltilerinden farklı hacimlerde eklenerek PON enzim aktivitesindeki değişiklikler tayin edildi. Farklı hacimlerde kullanılan Hg⁺² ağır metal iyonunun PON enzim aktiviteleri 30,9383 U/mLdak, 29,0598 U/mLdak, 26,3799 U/mLdak, 23,9443 U/mLdak, 20,6725 U/mLdak olarak hesaplandı, farklı hacimlerde kullanılan Cu⁺² ağır metal iyonunun PON enzim aktiviteleri 19,7949 U/mLdak, 19,4807 U/mLdak 19,1864 U/mLdak, 19,1200 U/mLdak, 18,9037 U/mLdak olarak hesaplandı ve farklı hacimlerde kullanılan Zn⁺² ağır metal iyonunun PON enzim aktiviteleri 23,8305 U/mLdak, 23,0781 U/mLdak, 22,9073 U/mLdak, 22,4324 U/mLdak, 21,8159 U/mLdak olarak hesaplandı. Elde edilen bu veriler sonucunda % aktivite değerleri hesaplanarak % aktivite grafikleri çizildi. Çalışma sonucunda Cu⁺² ve Zn⁺² ağır metal iyonlarının artan derişimlerinin enzim aktivitesinde azalmaya neden olduğu fakat kullanılan farklı derişimler arasında istatistiksel olarak anlamlı bir fark olmadığı belirlendi. Hg⁺² ağır metal iyonun artan derişimlerinin PON enzim aktivitesini inhibe ettiği, kullanılan farklı derişimlere bağlı olarak aktiviteler arasında istatistiksel olarak anlamlı bir azalmaya neden olduğu belirlendi (p < 0.05).

Anahtar Kelimeler

Supporting Institution

Ondokuz Mayıs Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı

Project Number

PYO.VET.1904.19.011

Thanks

Ondokuz Mayıs Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı'nın PYO.VET.1904.19.011 proje numarasıyla verdiği desteğe teşekkür ederiz.

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Details

Primary Language

English

Subjects

Structural Biology , Fisheries Management

Journal Section

Research Article

Authors

Büşra Şahin ^*
0000-0002-5245-478X
Türkiye

Sena Çenesiz
0000-0002-3544-503X
Türkiye

Publication Date

December 15, 2021

Submission Date

April 21, 2021

Acceptance Date

October 14, 2021

Published in Issue

Year 2021 Volume: 38 Number: 4

DOI

https://doi.org/10.12714/egejfas.38.4.09

IZ

https://izlik.org/JA98EN39EL

Cite

RIS / Bibtex

APA

Şahin, B., & Çenesiz, S. (2021). The effect of mercury, copper, and zinc on paraoxonase (PON) enzyme activity in Bonito (Sarda sarda) fish. Ege Journal of Fisheries and Aquatic Sciences, 38(4), 479-486. https://doi.org/10.12714/egejfas.38.4.09

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