The role of nitrosative and oxidative stress in rainbow trout (Oncorhynchus mykiss) liver tissue applied mercury chloride (HgCl2)

Mehmet Reşit Taysı; Muammer Kırıcı; Mahinur Kırıcı; Hasan Ulusal; Bünyamin Sögüt; Seyithan Taysı

doi:10.12714/egejfas.38.3.02

EN

The role of nitrosative and oxidative stress in rainbow trout (Oncorhynchus mykiss) liver tissue applied mercury chloride (HgCl2)

Abstract

The aim of this study was to determine oxidative stress caused by mercury chloride (HgCl₂) in rainbow trout (Oncorhynchus mykiss) liver tissue. For this purpose, the LD₅₀value of HgCl₂ on rainbow trout was determined as 551 μg/L. In the study, 40 fish in four groups were exposed to 25% and 50% (138 and 276 µg/L) of the two subletal doses of HgCl₂for 2 and 7 days, with 10 fish (n=10) in each group. To determine oxidative stress; peroxynitrite (ONOO−), total oxidant level (TOS), total antioxidant level (TAS), oxidative stress index (OSI) and malondialdehyde (MDA) were analyzed. In the study, it was observed that the differences between the groups in terms of ONOO−, TOS, TAS and OSI levels in the liver tissues was significant (P<0.05), however, this difference was not significant (P>0.05) in terms of MDA values. As a result, it can be concluded that HgCl₂ increases ONOO−, TOS, TAS, OSI and MDA levels in liver tissue and even small doses of mercury are toxic to fish.

Keywords

References

Ahlatci, A., Kuzhan, A., Taysi, S., Demirtas, O.C., Alkis, H.E., Tarakcioglu, M., Demirci, A., Caglayan, D., Saricicek, E. & Cinar, K. (2014). Radiation-modifying abilities of Nigella sativa and thymoquinone on radiation-induced nitrosative stress in the brain tissue. Phytomedicine 21, 740-744. DOI: 10.1016/j.phymed.2013.10.023
Al-Nimer, M.S., Al-Ani F.S. & Ali F.S. (2012). Role of nitrosative and oxidative stress in neuropathy in patients with type 2 diabetes mellitus. Journal of Neurosciences in Rural Practice 3, 41-44. DOI: 10.4103/0976-3147.91932
Bano, Y. & Hasan M. (1990). Histopathological lesions in the body organs of cat-fish (Heteropneustes fossilis) following mercury intoxication. Journal of Environmental Science and Health, Part B 25, 67-85. DOI: 10.1080/03601239009372677
Baser, S., Erkoc, F., Selvi, M. & Kocak, O. (2003). Investigation of acute toxicity of permethrin on guppies Poecilia reticulata. Chemosphere 51, 469-474. DOI: 10.1016/S0045-6535(03)00033-X
Berntssen, M.H.G., Aatland, A. & Handy, R.D. (2003). Chronic dietary mercury exposure causes oxidative stress, brain lesions, and altered behaviour in Atlantic salmon (Salmo salar) parr. Aquatic Toxicology 65, 55-72. DOI: 10.1016/S0166-445X(03)00104-8
Boudou, A. & Ribeyre, F. (1983). Contamination of aquatic biocenoses by mercury compounds an experimental ecotoxicological approach. In J. O. Nriagu (Ed.) Aquatic Toxicology, J Wiley and Sons (New York), pp. 117-136.
Chaudiere, J. & Ferrari-Iliou, R. (1999). Intracellular antioxidants: from chemical to biochemical mechanisms. Food and Chemical Toxicology 37, 949-962. DOI: 10.1016/S0278-6915(99)00090-3
de Oliveira Ribeiro, C.A., Belger, L., Pelletier, E. & Rouleau, C. (2002). Histopathological evidence of inorganic mercury and methyl mercury toxicity in the arctic charr (Salvelinus alpinus). Environmental Research 90, 217-225. DOI: 10.1016/S0013-9351(02)00025-7

Doğan, N., Yazıcı, Z. & Şişman, T. (2011). Lepistes balığının karaciğeri üzerine fenpiroksimat akarisiti’nin biyokimyasal etkileri. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 13: 1-8.
Doyotte, A., Cossu, C., Jacquin, M. C., Babut, M. & Vasseur, P. (1997). Antioxidant enzymes, glutathione and lipid peroxidation as relevant biomarkers of experimental or field exposure in the gills and the digestive gland of the freshwater bivalve Unio tumidus. Aquatic Toxicology, 39(2), 93-110. doi:Doi 10.1016/S0166-445x(97)00024-6
Driscoll, C. T., Yan, C., Schofiel, L., Munson, R. & Holsapple, J. (1994). The mercury cycle and fish in the adirondak lakes. Environmental Science and Technology, 28, 136-143. DOI:10.1021/es00052a003
Dural, M., Goksu, M. Z. L. & Ozak, A. A. (2007). Investigation of heavy metal levels in economically important fish species captured from the Tuzla lagoon. Food Chemistry, 102(1), 415-421. DOI: 10.1016/j.foodchem.2006.03.001
Elia, A. C., Galarini, R., Taticchi, M. I., Dorr, A. J. & Mantilacci, L. (2003). Antioxidant responses and bioaccumulation in Ictalurus melas under mercury exposure. Ecotoxicology and Environmental Safety, 55(2), 162-167. DOI: 10.1016/s0147-6513(02)00123-9
Erel, O. (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry, 37(4), 277-285. DOI: 10.1016/j.clinbiochem.2003.11.015
Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38(12), 1103-1111. DOI: 10.1016/j.clinbiochem.2005.08.008
Fathi, M., Binkowski, L. J., Azadi, N. A., Hamesadeghi, U. & Mansouri, B. (2018). Co-exposure effects of mercury chloride (HgCl2) and silver nanoparticles (Ag-NPs) on goldfish (Carassius auratus): Histopathological changes, oxidative stress response, and bioaccumulation. Desalination and Water Treatment, 105, 264-272. DOI: 10.5004/dwt.2018.21994
Gilbert, S. G. & Grantwebster, K. S. (1995). Neurobehavioral effects of developmental methylmercury exposure. Environmental Health Perspectives, 103, 135-142. DOI: 10.2307/3432363
Guardiola, F. A., Chaves-Pozo, E., Espinosa, C., Romero, D., Meseguer, J., Cuesta, A. & Esteban, M. A. (2016). Mercury accumulation, structural damages, and antioxidant and immune status changes in the gilthead seabream (Sparus aurata L.) exposed to methylmercury. Archives of Environmental Contamination and Toxicology, 70(4), 734-746. DOI: 10.1007/s00244-016-0268-6
Gundacker, C., Komarnicki, G., Zodl, B., Forster, C., Schuster, E. & Wittmann, K. (2006). Whole blood mercury and selenium concentrations in a selected Austrian population: Does gender matter? Science of the Total Environment, 372(1), 76-86. DOI: 10.1016/j.scitotenv.2006.08.006
Gunes, M., Sokmen, T. O. & Kirici, M. (2019). Determination of some metal levels in water, sediment and fish species of Tercan Dam Lake, Turkey. Applied Ecology and Environmental Research, 17(6), 14961-14972. DOI: 10.15666/aeer/1706_1496114972
Gül, A., Yılmaz, M. & Selvi, M. (2004). The study of the toxic effects of mercury-II-chloride. Gazi Üniversitesi Fen Bilimleri Dergisi, 17(4), 53-58.
Gül, A., Yılmaz, M. & Uzel, N. (2008). Acute toxic effect of mercury II chloride in Capoeta tinca (Heckel. 1843), living in the Kirmir stream of Sakarya river. Kastamonu Educational Journal, 16, 199-206.
Güven, A., Kahvecioğlu, Ö., Kartal, G. & Timur, S. (2004). Metallerin çevresel etkileri- III. Metalurji Dergisi 17(138), 64-71.
Has-Schon, E., Bogut, I., Vukovic, R., Galovic, D., Bogut, A. & Horvatic, J. (2015). Distribution and age-related bioaccumulation of lead (Pb), mercury (Hg), cadmium (Cd), and arsenic (As) in tissues of common carp (Cyprinus carpio) and European catfish (Sylurus glanis) from the Busko Blato reservoir (Bosnia and Herzegovina). Chemosphere, 135, 289-296. DOI: 10.1016/j.chemosphere.2015.04.015
Ibrahim, A. T. A. (2015). Effects of mercury chloride on oxidative stress biomarkers of some tissues of the african catfish Clarias gariepinus (Burchell, 1822). Journal of Veterinary Science and Technology, 6(4), 1-5. DOI: 10.4172/2157-7579.1000242
Kasassi, A., Rakimbei, P., Karagiannidis, A., Zabaniotou, A., Tsiouvaras, K., Nastis, A. & Tzafeiropoulou, K. (2008). Soil contamination by heavy metals: Measurements from a closed unlined landfill. Bioresource Technology, 99(18), 8578-8584. DOI: 10.1016/j.biortech.2008.04.010
Kaya, İ., Yılmaz, M., Koç, E., Deveci, H. A., Ersan, Y. & Karapehlivan, M. (2014). Tebukonazol (fungusit) uygulanan Cyprinus carpio (L. 1758)’da serum total antioksidan, oksidan ve sialik asit düzeylerinin incelenmesi. Journal of FisheriesSciences.com, 8(3), 214-219.
Kırıcı, M., Taysi, M. R., Bengü, A. Ş. & İspir, Ü. (2013). Determination of accumulation concentrations of some metals in the muscle tissue of Capoeta trutta (Heckel, 1843) captured in the Murat River. Journal of Erzincan University Institute of Science, 6(1), 115-124.
Iliopoulou-Georgudaki, J. & Kotsanis, N. (2001). Toxic effects of cadmium and mercury in rainbow trouth (Oncorhynchus mykiss): a short-term bioassay. Bulletin of Environmental Contamination and Toxicology. 66, 77-85. DOI: 10.1007/s0012800208
Maulvault, A. L., Custodio, A., Anacleto, P., Repolho, T., Pousao, P., Nunes, M. L. & Marques, A. (2016). Bioaccumulation and elimination of mercury in juvenile seabass (Dicentrarchus labrax) in a warmer environment. Environmental Research, 149, 77-85. DOI: 10.1016/j.envres.2016.04.035
Mieiro, C. L., Ahmad, I., Pereira, M. E., Duarte, A. C. & Pacheco, M. (2010). Antioxidant system breakdown in brain of feral golden grey mullet (Liza aurata) as an effect of mercury exposure. Ecotoxicology, 19(6), 1034-1045. DOI: 10.1007/s10646-010-0485-0
Morales, A. E., Pèrez-Jimènez, A., Hidalgo, M. C., Abellán, E. & Gabriel, C. G. (2004). Oxidative stres and antioxidant defenses after prolonged starvation in Dentex dentex liver. Comporative Biochemistry and Physiology, 139(7), 153-161.
Ohkawa, H., Ohishi, N. & Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95(2), 351-358. DOI: 10.1016/0003-2697(79)90738-3
Pandey, S., Kumar, R., Sharma, S., Hagpure, N.S., Srivastava, S.K., Verma, M.S. (2005). Acute toxicity bioassays of mercuric chloride and malathion on air-breathing fish Channa punctatus (Bloch). Ecotoxicology and Environmental Safety 61: 114-120. DOI: 10.1016/j.ecoenv.2004.08.004
Perottoni, J., Lobato, L. P., Silveira, A., Batista, J., Rocha, T. & Emanuelli, T. (2004). Effects of mercury and selenite on delta-aminolevulinate dehydratase activity and on selected oxidative stress parameters in rats. Environmental Research, 95(2), 166-173. DOI: 10.1016/j.envres.2003.08.007
Plessi, M., Bertelli, D. & Monzani, A. (2001). Mercury and selenium content in selected seafood. Journal of Food Composition and Analysis, 14, 461-467. DOI:10.1006/jfca.2001.1003
Powell, C. L., Swenberg, J. A. & Rusyn, I. (2005). Expression of base excision DNA repair genes as a biomarker of oxidative DNA damage. Cancer Letters, 229(1), 1-11. DOI: 10.1016/j.canlet.2004.12.002
Svobodova, Z., Luskova, V., Drastichova, J., Svoboda, M. & Zlabek, V. (2003). Effect of deltamethrin on haematological indices of common carp (Cyprinus carpio L.). Acta Veterinaria Brno, 72(1), 79-85. DOI: 10.2754/avb200372010079
Terzi, E. & Verep, B. (2012). Effects of water hardness and temperature on the acute toxicity of mercuric chloride on rainbow trout (Oncorhynchus mykiss). Toxicology and Industrial Health, 28(6), 499-504. DOI: 10.1177/0748233711416943
Thirumavalavan, R. (2010). Effect of mercury on lipid peroxidation and antioxidants in gill tissue of fresh water fish, labeo rohita. International Journal of Recent Scientific Research, 5, 122-124.
Thongra-ar, W., Parkpian, P. & Tang, A. (2003). Toxicity of mercury to growth and survival of seabass larvae. Lates carcarifer. and the modyfing of effects of salinity. ScienceAsia, 29, 2009-2019.
Vanuffelen, B. E., Van Der Zee, J., De Koster, B. M., Vansteveninck, J. & Elferink, J. G. (1998). Intracellular but not extracellular conversion of nitroxyl anion into nitric oxide leads to stimulation of human neutrophil migration. Biochemical Journal, 330(2), 719-722. DOI: 10.1042/bj3300719
Veena, K. B., Radhakrishnan, C. K. & Chacko, J. (1997). Heavy metal induced biochemical effects in an estuarine teleost. Indian Journal of Marine Sciences, 26(1), 74-78.
Verep, B., Besli, E. S., Altinok, I. & Mutlu, C. (2007). Assessment of mercuric chloride toxicity on rainbow trouts (Oncorhynchus mykiss) and chubs (Alburnoides bipunctatus). Pakistan Journal of Biological Sciences, 10(7), 1098-1102. DOI: 10.3923/pjbs.2007.1098.1102
Wang, B., Feng, L., Jiang, W. D., Wu, P., Kuang, S. Y., Jiang, J. & Zhou, X. Q. (2015). Copper-induced tight junction mRNA expression changes, apoptosis and antioxidant responses via NF-kappa B, TOR and Nrf2 signaling molecules in the gills of fish: Preventive role of arginine. Aquatic Toxicology, 158, 125-137. DOI:10.1016/j.aquatox.2014.10.025
Yonar, M. E., Ispir, U., Mise Yonar, S. & Kirici, M. (2016). Effect of copper sulphate on the antioxidant parameters in the rainbow trout fry, Oncorhynchus mykiss. Cellular and Molecular Biology, 62(6), 55-58.

Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Mehmet Reşit Taysı ^*
0000-0002-1072-4059
Türkiye

Muammer Kırıcı
0000-0003-1888-4388
Türkiye

Mahinur Kırıcı
0000-0003-4642-7387
Türkiye

Hasan Ulusal
0000-0003-3890-2088
Türkiye

Bünyamin Sögüt
0000-0002-7644-7226
Türkiye

Seyithan Taysı
0000-0003-1251-3148
Türkiye

Publication Date

September 15, 2021

Submission Date

December 23, 2020

Acceptance Date

April 1, 2021

Published in Issue

Year 1970 Volume: 38 Number: 3

DOI

https://doi.org/10.12714/egejfas.38.3.02

IZ

https://izlik.org/JA75KY74MH

Cite

RIS / Bibtex

APA

Taysı, M. R., Kırıcı, M., Kırıcı, M., Ulusal, H., Sögüt, B., & Taysı, S. (2021). The role of nitrosative and oxidative stress in rainbow trout (Oncorhynchus mykiss) liver tissue applied mercury chloride (HgCl2). Ege Journal of Fisheries and Aquatic Sciences, 38(3), 269-273. https://doi.org/10.12714/egejfas.38.3.02