Characterization of polyextremotolerant bacterial strains (Kocuria, Virgibacillus, and Halomonas) with industrial potential isolated from the İzmir Çamaltı Saltern

Ebru Tekin; Mustafa Ateş

doi:10.12714/egejfas.42.4.04

TR EN

İzmir Çamaltı Tuzlası’ndan izole edilen endüstriyel potansiyele sahip poliekstremotolerant bakteri suşlarının (Kocuria, Virgibacillus ve Halomonas) karakterizasyonu

Abstract

Bu çalışmada, endüstriyel açıdan önemli halotolerant bakteri suşlarının İzmir Çamaltı Tuzlası’ndan alınan deniz suyu ve yığın örneklerinden izolasyonu, saflaştırılması ve tanılanması gerçekleştirilmiştir. Seçilen üç izolat, fenotipik, biyokimyasal ve kısmi 16S rRNA dizi analizleri ile kapsamlı şekilde incelenmiş ve türler erişim numaraları alınarak GenBank’a kayıt edilmiştir.Türe özgü filogenetik ağaçlar MEGA6.06 programı kullanılarak oluşturulmuştur. Suşların sırasıyla Firmicutes, Actinobacteria ve Gammaproteobacteria filumlarına; %95 benzerlik ile Virgibacillus, %99 benzerlik ile Kocuria ve %99 benzerlik ile Halomonas cinslerine ait olduğu belirlenmiştir. Suşlar Kocuria sp. CT-6 (KP238667), Virgibacillus sp. CT-5 (KP238666) ve Halomonas sp. CS-5 (KP238669) olarak adlandırılmıştır. Fenotipik karakterizasyona göre Virgibacillus sp. CT-5 termotolerant olup 20-55 °C aralığında büyüyebilmektedir; Kocuria sp. CT-6 psikrotolerant olup 4-37 °C aralığında yaşamaktadır; Halomonas sp. CS-5 ise hem psikrotolerant hem termotolerant olup 4-45 °C aralığında gelişim göstermektedir. Tüm suşlar alkalitolerant olup pH 5-11 aralığında büyüyebilmekte; halotolerant olup sırasıyla Virgibacillus sp. CT-5 için %0-22 (w/v), Kocuria sp. CT-6 için %0-19 (w/v) ve Halomonas sp. CS-5 için %0-25 (w/v) NaCl konsantrasyonlarında gelişim gösterebilmektedir. Bu çalışmada, İzmir Çamaltı Tuzlası’ndan izole edilen üç poliekstremotolerant bakteri suşu- Kocuria sp. CT-6, Virgibacillus sp. CT-5 ve Halomonas sp. CS-5- bildirilmiştir. Bu suşlar, birden fazla tolerans özelliğine sahip olup, endüstriyel uygulamalar için büyük bir öneme sahiptir. Bu çalışma, bu bölgeden poliekstremotolerant suşların rapor edildiği ilk çalışmadır.

Keywords

Characterization of polyextremotolerant bacterial strains (Kocuria, Virgibacillus, and Halomonas) with industrial potential isolated from the İzmir Çamaltı Saltern

Abstract

In this study, industrially important halotolerant bacterial strains were isolated and identified from seawater and bulk samples from the İzmir Çamaltı Saltern. Three selected isolates underwent comprehensive analyses including phenotypic, biochemical, and partial 16S rRNA gene sequencing and accession numbers were taken from GenBank. Species specific trees were constructed using MEGA 6.06 software. The isolates were classified within the phyla Firmicutes, Actinobacteria, and Gammaproteobacteria, exhibiting highest sequence similarity to the genera Virgibacillus (95%), Kocuria (99%), and Halomonas (99%), respectively. The strains were designated as Virgibacillus sp. CT-5 (KP238666), Kocuria sp. CT-6 (KP238667), and Halomonas sp. CS-5 (KP238669). Phenotypic characterization revealed that Virgibacillus sp. CT-5 is thermotolerant, growing at 20–55 °C; Kocuria sp. CT-6 is psychrotolerant, growing at 4-37 °C; and Halomonas sp. CS-5 exhibits both psychrotolerance and thermotolerance, growing at 4-45 °C. All strains were alkalitolerant, thriving within a pH range of 5-11, and halotolerant, with growth observed at 0-22% (w/v) NaCl for Virgibacillus sp. CT-5, 0-19% (w/v) for Kocuria sp. CT-6, and 0-25% (w/v) for Halomonas sp. CS-5. This study presents three polyextremotolerant bacterial strains -Kocuria sp. CT-6, Virgibacillus sp. CT-5, and Halomonas sp. CS-5- isolated from the İzmir Çamaltı Saltern. Their multiple tolerance traits render them highly promising candidates for various industrial applications. This is the first report documenting the polyextremotolerance of these strains from this area.

Keywords

Supporting Institution

Ege University Scientific Research Council

Project Number

2011 FEN 010

Ethical Statement

NEthical approval was not necessary for the study.

Thanks

We are thankful for the support of Ege University Scientific Research Council.

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Details

Primary Language

English

Subjects

Marine and Estuarine Ecology, Fish Pests and Diseases

Journal Section

Research Article

Authors

Ebru Tekin ^*
0000-0003-2623-1977
Türkiye

Mustafa Ateş
0000-0002-9305-3480
Türkiye

Early Pub Date

December 1, 2025

Publication Date

December 10, 2025

Submission Date

June 23, 2025

Acceptance Date

September 17, 2025

Published in Issue

Year 2025 Volume: 42 Number: 4

DOI

https://doi.org/10.12714/egejfas.42.4.04

IZ

https://izlik.org/JA55XE44UF

Cite

RIS / Bibtex

APA

Tekin, E., & Ateş, M. (2025). Characterization of polyextremotolerant bacterial strains (Kocuria, Virgibacillus, and Halomonas) with industrial potential isolated from the İzmir Çamaltı Saltern. Ege Journal of Fisheries and Aquatic Sciences, 42(4), 307-316. https://doi.org/10.12714/egejfas.42.4.04

AMA

1.Tekin E, Ateş M. Characterization of polyextremotolerant bacterial strains (Kocuria, Virgibacillus, and Halomonas) with industrial potential isolated from the İzmir Çamaltı Saltern. EgeJFAS. 2025;42(4):307-316. doi:10.12714/egejfas.42.4.04

Chicago

Tekin, Ebru, and Mustafa Ateş. 2025. “Characterization of Polyextremotolerant Bacterial Strains (Kocuria, Virgibacillus, and Halomonas) With Industrial Potential Isolated from the İzmir Çamaltı Saltern”. Ege Journal of Fisheries and Aquatic Sciences 42 (4): 307-16. https://doi.org/10.12714/egejfas.42.4.04.

EndNote

Tekin E, Ateş M (December 1, 2025) Characterization of polyextremotolerant bacterial strains (Kocuria, Virgibacillus, and Halomonas) with industrial potential isolated from the İzmir Çamaltı Saltern. Ege Journal of Fisheries and Aquatic Sciences 42 4 307–316.

IEEE

[1]E. Tekin and M. Ateş, “Characterization of polyextremotolerant bacterial strains (Kocuria, Virgibacillus, and Halomonas) with industrial potential isolated from the İzmir Çamaltı Saltern”, EgeJFAS, vol. 42, no. 4, pp. 307–316, Dec. 2025, doi: 10.12714/egejfas.42.4.04.

ISNAD

Tekin, Ebru - Ateş, Mustafa. “Characterization of Polyextremotolerant Bacterial Strains (Kocuria, Virgibacillus, and Halomonas) With Industrial Potential Isolated from the İzmir Çamaltı Saltern”. Ege Journal of Fisheries and Aquatic Sciences 42/4 (December 1, 2025): 307-316. https://doi.org/10.12714/egejfas.42.4.04.

JAMA

1.Tekin E, Ateş M. Characterization of polyextremotolerant bacterial strains (Kocuria, Virgibacillus, and Halomonas) with industrial potential isolated from the İzmir Çamaltı Saltern. EgeJFAS. 2025;42:307–316.

MLA

Tekin, Ebru, and Mustafa Ateş. “Characterization of Polyextremotolerant Bacterial Strains (Kocuria, Virgibacillus, and Halomonas) With Industrial Potential Isolated from the İzmir Çamaltı Saltern”. Ege Journal of Fisheries and Aquatic Sciences, vol. 42, no. 4, Dec. 2025, pp. 307-16, doi:10.12714/egejfas.42.4.04.

Vancouver

1.Ebru Tekin, Mustafa Ateş. Characterization of polyextremotolerant bacterial strains (Kocuria, Virgibacillus, and Halomonas) with industrial potential isolated from the İzmir Çamaltı Saltern. EgeJFAS. 2025 Dec. 1;42(4):307-16. doi:10.12714/egejfas.42.4.04