Seasonal changes in antioxidant defense system indicators in the tissues of Cyprinion macrostomus (Heckel, 1843) caught from Göynük Stream (Bingöl, Turkey)

Muammer Kırıcı; Nurgül Sen Özdemir; Fatma Caf; Mustafa Koyun

doi:10.12714/egejfas.39.3.01

EN TR

Seasonal changes in antioxidant defense system indicators in the tissues of Cyprinion macrostomus (Heckel, 1843) caught from Göynük Stream (Bingöl, Turkey)

Abstract

In this study, antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase GPx), glutathione reductase (GR) and glucose 6-phosphate dehydrogenase (G6PD)) and malondialdehyde (MDA) levels occurring throughout the year were examined in Cyprinion macrostomus tissues (kidney, gill, liver and gonad) captured from Göynük Stream (Bingöl, Turkey). For this purpose, two locations (Ilıcalar and Garip) where fish can be caught regularly in summer, autumn, winter and spring were determined. Fish were caught regularly from these two locations every month and brought to the laboratory. Spectrophotometric methods were used to determine enzyme activities and MDA levels in the study. As a result of the study, it was determined that the MDA level and enzyme activities between Ilıcalar and Garip stations, in general, were statistically different from each other in all tissues. However, it was observed that there were important differences in general between the seasons at both stations. In addition, while GR and G6PD activities were lower than other enzyme activities throughout the study, CAT and SOD activities were higher.

Keywords

Göynük Çayı'nda (Bingöl) yakalanan Cyprinion macrostomus dokularında antioksidan savunma sistemi göstergelerindeki mevsimsel değişiklikler

Öz

Bu çalışmada, Göynük Çayı'ndan (Bingöl, Türkiye) yakalanan Cyprinion macrostomus dokularında (böbrek, solungaç, karaciğer ve gonad) yıl boyunca meydana gelen antioksidan enzim aktiviteleri (süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz GPx), glutatyon redüktaz (GR) ve glukoz 6-fosfat dehidrojenaz (G6PD)) ve malondialdehit (MDA) seviyelerindeki değişimler mevsimsel olarak incelenmiştir. Bu amaçla yaz, sonbahar, kış ve ilkbaharda düzenli olarak balık yakalanabilecek iki lokasyon (Ilıcalar ve Garip) belirlenmiştir. Bu iki lokasyondan her ay düzenli olarak balıklar yakalanarak laboratuvara getirilmiştir. Çalışmada enzim aktivitelerini ve MDA düzeylerini belirlemek için spektrofotometrik yöntemler kullanılmıştır. Çalışma sonucunda genel olarak Ilıcalar ve Garip istasyonları arasındaki MDA düzeyi ve enzim aktivitelerinin tüm dokularda istatistiksel olarak birbirinden farklı olduğu belirlendi. Bununla berarber, her iki istasyonda da mevsimler arasında genel olarak anlamlı farklılıkların olduğu gözlemlenmiştir. Ayrıca çalışmada, GR ve G6PD aktiviteleri diğer enzim aktivitelerinden daha düşük iken, CAT ve SOD aktivitelerinin diğer enzim aktivitelerinden daha yüksek olduğu tespit edilmiştir.

Anahtar Kelimeler

Supporting Institution

Bingöl Üniversitesi

Project Number

BAP-ZF.2016.00.006

Thanks

Bu çalışma, Bingöl Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir (Proje No: BAP-ZF.2016.00.006).

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Details

Primary Language

English

Subjects

Limnology

Journal Section

Research Article

Authors

Muammer Kırıcı ^*
0000-0003-1888-4388
Türkiye

Nurgül Sen Özdemir
0000-0001-6070-9765
Türkiye

Fatma Caf
0000-0002-0363-4848
Türkiye

Mustafa Koyun
0000-0002-9047-0430
Türkiye

Publication Date

September 15, 2022

Submission Date

March 1, 2022

Acceptance Date

May 11, 2022

Published in Issue

Year 2022 Volume: 39 Number: 3

DOI

https://doi.org/10.12714/egejfas.39.3.01

IZ

https://izlik.org/JA28BR77NF

Cite

RIS / Bibtex

APA

Kırıcı, M., Sen Özdemir, N., Caf, F., & Koyun, M. (2022). Seasonal changes in antioxidant defense system indicators in the tissues of Cyprinion macrostomus (Heckel, 1843) caught from Göynük Stream (Bingöl, Turkey). Ege Journal of Fisheries and Aquatic Sciences, 39(3), 174-181. https://doi.org/10.12714/egejfas.39.3.01

Cited By

Seasonal changes in antioxidant enzyme activities of Garra rufa (Heckel, 1843) in Göynük Stream (Bingöl, Türkiye)

Ege Journal of Fisheries and Aquatic Sciences

https://doi.org/10.12714/egejfas.40.3.03