Karyological analysis of endemic Pseudophoxinus anatolicus (Hankó 1925) in Türkiye

Ahmed Sadeq Jaber Doori; Atilla Arslan

doi:10.12714/egejfas.41.1.04

TR EN

Türkiye'ye endemik Pseudophoxinus anatolicus (Hankó 1925)'un karyolojik analizi

Öz

Türkiye’deki Pseudophoxinus türlerin yarısının karyolojik özellikleri belirtilmiştir. Beyşehir gölünde yaygın olan P. anatolicus’un karyolojik özelliklerini belirlemek için planlanan bu çalışmada örnekler Çiftlik köyü kıyısından yakalandı. Yakalanan bireylerin karyolojik analizleri yapıldı ve elde edilen slaytlara sırayla giemsa boyama, C-bantlama ve Ag-NOR boyama uygulandı. Bu türün kromozom seti 2 çift metasentrik, sekiz çift submetasentrik, iki çift subtelosentrik ve üç çift akrosentrik kromozomdan oluşmaktadır. Koyu ve açık C-bantlar bazı kromozomların sentromerik bölgelerinde gözlemlendi. Aktif Ag-NOR’lar iki çift kromozomun kısa kolunun telomerik bölgesinde tespit edildi. Sonuçlarımızın diğer Pseudophoxinus türlerinkine bazı farklılıklar hariç benzer tarafları vardır ve Anadolu yağ balığının da diğer Pseudophoxinus türlerindeki gibi korunmuş karyotipe sahip olduğu belirlendi.

Anahtar Kelimeler

Karyological analysis of endemic Pseudophoxinus anatolicus (Hankó 1925) in Türkiye

Abstract

The karyological characteristics of nearly half of the Pseudophoxinus species in Türkiye were determined. In this study, it is planned to determine the karyological characteristics of P. anatolicus, which is common in Beyşehir Lake, specimens were caught from the coast at Çiftlik village. The captured specimen were karyological analysed and Giemsa staining, C-banding and Ag-NOR staining were applied to the slides that obtained. The chromosome set of this species consists of 12 pairs of metacentric, eight pairs of submetacentric, two pairs of subtelocentric and three pairs of acrocentric chromosomes. Dark and slightly C-bands were observed in the centromeric regions of some chromosomes. Active Ag-NORs were detected in the telomeric region of the short arm of two pairs of chromosomes. Our results are similar to those of other Pseudophoxinus species except for some differences and it was determined that Anatolian minnow has a conserved karyotype like other Pseudophoxinus species.

Keywords

Destekleyen Kurum

This study was financed with a grant from the Scientific Research Projects Coordination Board of Selçuk University

Proje Numarası

21211009

Etik Beyan

The samples in this study were collected with the permission of the TR Ministry of Forestry and Water Affairs (Permit No: E-21264211-288.04-3435924). This permission replaces the local ethics committee permission per 8/L of the regulation "On Working Procedures and Principles of Animal Experiments Ethics Committees" prepared by the Ministry of Forestry and Water Affairs and published in the Official Gazette on February 15, 2014.

Teşekkür

The Scientific Research Projects Coordination Board of Selçuk University (BAP)

Kaynakça

Arslan, A., & Gündoğdu H. (2016). Cytogenetic studies on the endemic Beyşehir nase, Chondrostoma beysehirense (Bogutskaya, 1997) in Türkiye. Caryologia, 69(2), 116 120. https://doi.org/10.1080/00087114.2015.1109958
Bertollo, L., Cioffi, M., & Moreira-Filho, O. (2015). Direct chromosome preparation from freshwater teleost fishes. In C. Ozouf-Costaz, E. Pisano, F. Foresti, & L. Almeida Toledo (Eds.), Fish Cytogenetic Techniques (Chondrichthyans and Teleosts) (pp. 21-26). United States: CRC Press.
Bueno, V., Venere, P.C., Thums Konerat, J., Zawadzki, C.H., Vicari, M.R., & Margarido, V.P. (2014). Physical mapping of the 5S and 18S rDNA in ten species of Hypostomus Lacépède 1803 (Siluriformes: Loricariidae): evolutionary tendencies in the genus. Scientific World Journal, 943825, https://doi.org/10.1155/2014/943825
Çiçek, E., Sungur, S., & Fricke, R. (2020). Freshwater lampreys and fishes of Türkiye; a revised and updated annotated checklist 2020. Zootaxa, 4809(2), 241-270. https://doi.org/10.11646/zootaxa.4809.2.2
Diniz, D., Laudicina, A., & Bertollo, L.A. (2009). Chromosomal location of 18S and 5S rDNA sites in Triportheus fish species (Characiformes, Characidae). Genetics and Molecular Biology, 32, 37-41. https://doi.org/10.1590/S1415-47572009005000017
Ergene, S., Karahan, A., & Kuru, M. (2010). Cytogenetic analysis of Pseudophoxinus antalyae, Bogustkaya, 1992 (Pisces: Cyprinidae) from the Eastern Mediterranean River Basin. Türkiye. Turkish Journal of Zoology, 34(1), 111-117. https://doi.org/10.3906/zoo-0807-33
Gaffaroğlu, M., Karasu Ayata, M., & Ünal Karakuş, S. (2022). Cytogenetic characteristics of two species of the genus Pseudophoxinus Bleeker, 1860 (Cypriniformes: Leuciscidae) from Anatolia, Turkey. Acta Zoologica Bulgarica, 74(3), 335-342.
Howell, W.M., & Black, D.A. (1980). Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia, 36, 1014-1015. https://doi.org/10.1007/BF01953855

Karasu, M., Yüksel, E., & Gaffaroğlu, M. (2011). Karyotype, NORs, and C-banding analysis of Pseudophoxinus firati Bogutskaya, Küçük, & Atalay, 2007 (Actinopterygii, Cyprinidae) in the Euphrates River. Türkiye. Turkish Journal of Zoology, 35(6), 865-868. https://doi.org/10.3906/zoo-0912-129
Karasu Ayata, M., Yüksel, E., & Gaffaroğlu, M. (2016). Cytogenetic studies on six species of the leuciscine genus Pseudophoxinus Bleeker, 1860 (Teleostei, Cyprinidae). Caryologia, 69(3), 215 222. https://doi.org/10.1080/00087114.2016.1169089
Küçük, F., Atalay, M.A., Güçlü, S.S., & Gülle, İ. (2012). The Morphological Characteristics of Pseudophoxinus (Teleostei:Cyprinidae) Species of Turkey and their Zoogeographic Distribution. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 8(2), 1-9. (in Turkish with English abstract)
Küçük, F., Gülle, I.,& Güçlü, S.S. (2016). Pseudophoxinus iconii, a new species of spring minnow from Central Anatolia (Teleostei: Cyprinidae). Ichthyological Exploration of Freshwaters, 27(3), 283-288.
Levan, A., Fredga, K., & Sandberg, A.A. (1964). Nomenclature for centromeric position on chromosomes. Hereditas, 52, 201 220. https://doi.org/10.1111/j.1601-5223.1964.tb01953.x
Perea, S., Bohme, M., Zupancic, P., Freyhof, J., Sanda, R., Özulug, M., Abdoli, A., & Doadrio, I. (2010). Phylogenetic relationships and biogeographical patterns in Circum-Mediterranean subfamily Leuciscinae (Teleostei, Cyprinidae) inferred from both mitochondrial and nuclear data. BMC Evolutionary Biology 10, 265. https://doi.org/10.1186/1471-2148-10-265
Sumner, A. (1972). A simple technique for demonstrating centromeric heterochromatin. Experimental Cell Research, 75, 304 306. https://doi.org/10.1016/0014-4827(72)90558-7
Ünal, S., & Gaffaroğlu, M. (2016). Karyology of six cyprinid fishes from Seyhan and Ceyhan rivers in Anatolia. Caryologia, 69(4), 362-369. https://doi.org/10.1080/00087114.2016.1247328
Ünal, S, Gaffaroğlu, M., Karasu Ayata, M., & Yüksel, E. (2014). Karyotype, C-banding and AgNORs of two endemic leuciscine fish, Pseudophoxinus crassus (Ladiges, 1960) and P. hittitorum Freyhof & Özulug, 2010 (Teleostei, Cyprinidae). Comparative Cytogenetics, 8(4), 249-257. https://doi.org/10.3897/CompCytogen.v8i4.7623

Ayrıntılar

Birincil Dil

İngilizce

Konular

Ekoloji (Diğer)

Bölüm

Araştırma Makalesi

Yazarlar

Ahmed Sadeq Jaber Doori
0000-0002-7452-657X
Türkiye

Atilla Arslan ^*
0000-0002-4766-4969
Türkiye

Erken Görünüm Tarihi

11 Mart 2024

Yayımlanma Tarihi

15 Mart 2024

Gönderilme Tarihi

30 Ekim 2023

Kabul Tarihi

16 Ocak 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 41 Sayı: 1

DOI

https://doi.org/10.12714/egejfas.41.1.04

IZ

https://izlik.org/JA73LA62UD

Kaynak Göster

RIS / Bibtex

APA

Doori, A. S. J., & Arslan, A. (2024). Karyological analysis of endemic Pseudophoxinus anatolicus (Hankó 1925) in Türkiye. Ege Journal of Fisheries and Aquatic Sciences, 41(1), 26-29. https://doi.org/10.12714/egejfas.41.1.04

Cited By

Karyotype and Repetitive DNA Analysis in Turcichondrostoma fahirae (Cypriniformes, Leuciscidae): A Step toward the Use of Molecular Cytogenetics in Taxonomy of Freshwater Fishes in Türkiye

Cytogenetic and Genome Research

https://doi.org/10.1159/000544909