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Effects of myo-inositol concentration on growth and pigments of Nannochloropsis oculata culture

Yıl 2020, Cilt 37, Sayı 2, 195 - 199, 15.06.2020
https://doi.org/10.12714/egejfas.37.2.11

Öz



Inositols are used as growth promoting agents over plants. But microalgae are different from higher plant especially photosynthetic efficiency and productivity. According to the results of this study, myo-inositol addition to the culture medium of Nannochloropsis oculata provides higher cell densities. 100 mg L-1 myo-inositol added experimental group was reached to 1.42 fold cell mL-1, while the 500 mg L-1 myo-inositol added group was reached to 1.28 fold cell mL-1 than the control group. Mean chlorophyll a per cell amounts were calculated for experimental groups and control groups as 0.052 pg cell-1 and 0.053 pg cell-1, respectively. Mean total carotene per cell amounts were calculated for all groups as 0.016 pg cell-1. These results show that no difference was occurred between all groups by chlorophyll a and total carotene amounts per cell. This study shows that myo-inositol use in microalgae production may provide higher yields. 




Kaynakça

  • Brown, M. R., Mular, M., Miller, I., Farmer, C., & Trenerry, C., (1999). The vitamin content of microalgae used in aquaculture. Journal of Applied Phycology, 11(3):247-255. DOI: https://doi.org/10.1023/A:1008075903578
  • Cho, K., Kim, K. N., Lim, N. L., Kim, M. S., Ha, J. C., Shin, H. H., ... & Oda, T., (2015). Enhanced biomass and lipid production by supplement of myo-inositol with oceanic microalga Dunaliella salina. Biomass and Bioenergy. 72, 1-7. DOI: https://doi.org/10.1016/j.biombioe.2014.11.014
  • Chu, W. L., (2012). Biotechnological applications of microalgae, IeJSME, 6(1), S24-S37. https://www.crcpress.com/Biotechnological-Applications-of-Microalgae-Biodiesel-and-Value-Added/Bux/p/book/9781466515291
  • Dixit, R. B., & Suseela, M. R., (2013). Cyanobacteria: Potential candidates for drug discovery. Antonie van Leeuwenhoek. 103(5), 947-961. DOI: 10.1007/s10482-013-9898-0.
  • Durmaz, Y., Donato, M., Monteiro, M., Gouveia, L., Nunes, M.L., Gama Pereira, T., Gokpinar, S and Bandarra, N.M., (2008). Effect of Temperature on Growth and Biochemical Composition (Sterols, α-tocopherol, Carotenoids, Fatty Acid Profiles) of the Microalga, Isochrysis galbana. The Israeli Journal of Aquaculture – Bamidgeh 60(3):188-195. http://hdl.handle.net/10524/19257
  • Durmaz, Y. and Bandarra, NÇM., (2017). Fatty acids and Pigments Content of Nannochloropsis oculata (Eustigmatophyceae) Culture at Bag Systems Using Different Nitrogen Sources and Concentration in Medium. Fresenius Environmental Bulletin. 26/8; 5289-5294. DOI: https://www.prt-parlar.de/download_feb_2017/
  • Guillard, R. R., (1975). Culture of phytoplankton for feeding marine invertebrates. In Culture of marine invertebrate animals (pp. 29-60). Springer US. DOI: https://doi.org/10.1007/978-1-4615-8714-9_3
  • Loewus, F. A., & Loewus, M. W., (1983). Myo-inositol: its biosynthesis and metabolism. Annual Review of Plant Physiology. 34(1), 137-161. DOI: https://doi.org/10.1146/annurev.pp.34.060183.001033
  • Lubián, L. M., Montero, O., Moreno-Garrido, I., Huertas, I. E., Sobrino, C., González- del Valle, M., & Parés, G., (2000). Nannochloropsis (Eustigmatophyceae) as source of commercially valuable pigments. Journal of Applied Phycology. 12(3), 249-255. DOI: https://doi.org/10.1023/A:1008170915932
  • Morre, D. J., Boss, W. F., & Loewus, F. A., (Eds.) (1990). Inositol metabolism in plants (Vol. 9). New York, NY: Wiley-Liss.
  • Mortensen, A., (2006). Carotenoids and other pigments as natural colorants. Pure and Applied Chemistry. 78(8), 1477-1491. DOI: https://doi.org/10.1351/pac200678081477
  • Otero, A., García, D., & Fábregas, J., (1997). Factors controlling eicosapentaenoic acid production in semicontinuous cultures of marine microalgae. Journal of applied phycology, 9(5), 465-469. DOI: https://doi.org/10.1023/A:1007930804367
  • Pulz, O., & Gross, W., (2004). Valuable products from biotechnology of microalgae. Applied microbiology and biotechnology. 65(6), 635-648. DOI:10.1007/s00253-004-1647-x
  • Richmond, A., (2004). Principles for attaining maximal microalgal productivity in photobioreactors: an overview. Hydrobiologia, 512(1-3):33-37. DOI: https://doi.org/10.1023/B:HYDR.0000020365.06145.36
  • Sánchez, M. D., Mantell, C., Rodrıguez, M., de La Ossa, E. M., Lubián, L. M., & Montero, O., (2005). Supercritical fluid extraction of carotenoids and chlorophyll a from Nannochloropsis gaditana. Journal of Food Engineering. 66(2), 245-251. DOI: https://doi.org/10.1016/j.jfoodeng.2004.03.021
  • Stevenson, J. M., Perera, I. Y., Heilmann, I., Persson, S., & Boss, W. F., (2000). Inositol signaling and plant growth. Trends in plant science. 5(6), 252-258. DOI: https://doi.org/10.1016/S1360-1385(00)01652-6
  • Vallurua, R.; Van den Ende, W., (2011). Myo-inositol and beyond – Emerging networks under stress. Plant Science, 181, 387–400. DOI: 10.1016/j.plantsci.2011.07.009.
  • Vismara, R., Vestri, S., Kusmic, C., Barsanti, L., & Gualtieri, P., (2003). Natural vitamin E enrichment of Artemia salina fed freshwater and marine microalgae. Journal of applied phycology, 15(1):75-80. DOI: https://doi.org/10.1023/A:1022942705496
  • Volkman, J. K., Brown, M. R., Dunstan, G. A., & Jeffrey, S. W., (1993). The biochemical composition of marine microalgae from the class Eustigmatophyceae. Journal of Phycology. 29(1), 69-78. DOI: https://doi.org/10.1111/j.1529-8817.1993.tb00281.x
  • Zar, J.H., (1999). Biostatistical Analysis. Upper Saddle River, Prentice Hall, New Jersey, 4th Edition. cap 12, USA, 231-272. https://trove.nla.gov.au/work/7899770
  • Zou, N., & Richmond, A., (2000). Light-path length and population density in photoacclimation of Nannochloropsis sp.(Eustigmatophyceae). Journal of Applied Phycology. 12(3-5), 349-354. DOI: https://doi.org/10.1023/A:1008151004317

Myo-inositol konsantrasyonunun Nannochloropsis oculata kültüründe büyüme ve pigmentleri üzerine etkileri

Yıl 2020, Cilt 37, Sayı 2, 195 - 199, 15.06.2020
https://doi.org/10.12714/egejfas.37.2.11

Öz



İnositoller, bitkilerde büyüme arttırıcı olarak kullanılmaktadır. Ancak, mikroalgler yüksek bitkilerden özellikle fotosentetik etkinlik ve üretkenlik açısından farklıdır. Bu çalışmada elde edilen verilere göre Nannochloropsis oculata kültür ortamına myo-inositol ilave edilmesi daha yüksek hücre yoğunluğuna neden olmaktadır. 100 mg L-1 myo-inositol ilave edilen grup kontrol grubuna göre 1,42 kat hücre mL-1, 500 mg L-1 myo-inositol ilave edilen grup ise kontrol grubuna göre 1,28 kat hücre mL-1 yoğunluğa ulaşmıştır. Hücre başına ortalama klorofil a değerleri ise deneme grupları ve kontrol grubu için sırasıyla 0,052 pg hücre-1 ve 0,053 pg hücre-1 olarak hesaplanmıştır. Hücre başına ortalama toplam karoten miktarları ise tüm gruplar için 0,016 pg hücre-1 olarak hesaplanmıştır. Bu sonuçlar gruplar arasında hücre başına klorofil a ve toplam karoten miktarlarının değişmediğini göstermektedir. Bu çalışma, mikroalg üretiminde myo-inositol kullanımının daha fazla ürün elde edilmesini sağlayabileceğini ortaya koymaktadır.




Kaynakça

  • Brown, M. R., Mular, M., Miller, I., Farmer, C., & Trenerry, C., (1999). The vitamin content of microalgae used in aquaculture. Journal of Applied Phycology, 11(3):247-255. DOI: https://doi.org/10.1023/A:1008075903578
  • Cho, K., Kim, K. N., Lim, N. L., Kim, M. S., Ha, J. C., Shin, H. H., ... & Oda, T., (2015). Enhanced biomass and lipid production by supplement of myo-inositol with oceanic microalga Dunaliella salina. Biomass and Bioenergy. 72, 1-7. DOI: https://doi.org/10.1016/j.biombioe.2014.11.014
  • Chu, W. L., (2012). Biotechnological applications of microalgae, IeJSME, 6(1), S24-S37. https://www.crcpress.com/Biotechnological-Applications-of-Microalgae-Biodiesel-and-Value-Added/Bux/p/book/9781466515291
  • Dixit, R. B., & Suseela, M. R., (2013). Cyanobacteria: Potential candidates for drug discovery. Antonie van Leeuwenhoek. 103(5), 947-961. DOI: 10.1007/s10482-013-9898-0.
  • Durmaz, Y., Donato, M., Monteiro, M., Gouveia, L., Nunes, M.L., Gama Pereira, T., Gokpinar, S and Bandarra, N.M., (2008). Effect of Temperature on Growth and Biochemical Composition (Sterols, α-tocopherol, Carotenoids, Fatty Acid Profiles) of the Microalga, Isochrysis galbana. The Israeli Journal of Aquaculture – Bamidgeh 60(3):188-195. http://hdl.handle.net/10524/19257
  • Durmaz, Y. and Bandarra, NÇM., (2017). Fatty acids and Pigments Content of Nannochloropsis oculata (Eustigmatophyceae) Culture at Bag Systems Using Different Nitrogen Sources and Concentration in Medium. Fresenius Environmental Bulletin. 26/8; 5289-5294. DOI: https://www.prt-parlar.de/download_feb_2017/
  • Guillard, R. R., (1975). Culture of phytoplankton for feeding marine invertebrates. In Culture of marine invertebrate animals (pp. 29-60). Springer US. DOI: https://doi.org/10.1007/978-1-4615-8714-9_3
  • Loewus, F. A., & Loewus, M. W., (1983). Myo-inositol: its biosynthesis and metabolism. Annual Review of Plant Physiology. 34(1), 137-161. DOI: https://doi.org/10.1146/annurev.pp.34.060183.001033
  • Lubián, L. M., Montero, O., Moreno-Garrido, I., Huertas, I. E., Sobrino, C., González- del Valle, M., & Parés, G., (2000). Nannochloropsis (Eustigmatophyceae) as source of commercially valuable pigments. Journal of Applied Phycology. 12(3), 249-255. DOI: https://doi.org/10.1023/A:1008170915932
  • Morre, D. J., Boss, W. F., & Loewus, F. A., (Eds.) (1990). Inositol metabolism in plants (Vol. 9). New York, NY: Wiley-Liss.
  • Mortensen, A., (2006). Carotenoids and other pigments as natural colorants. Pure and Applied Chemistry. 78(8), 1477-1491. DOI: https://doi.org/10.1351/pac200678081477
  • Otero, A., García, D., & Fábregas, J., (1997). Factors controlling eicosapentaenoic acid production in semicontinuous cultures of marine microalgae. Journal of applied phycology, 9(5), 465-469. DOI: https://doi.org/10.1023/A:1007930804367
  • Pulz, O., & Gross, W., (2004). Valuable products from biotechnology of microalgae. Applied microbiology and biotechnology. 65(6), 635-648. DOI:10.1007/s00253-004-1647-x
  • Richmond, A., (2004). Principles for attaining maximal microalgal productivity in photobioreactors: an overview. Hydrobiologia, 512(1-3):33-37. DOI: https://doi.org/10.1023/B:HYDR.0000020365.06145.36
  • Sánchez, M. D., Mantell, C., Rodrıguez, M., de La Ossa, E. M., Lubián, L. M., & Montero, O., (2005). Supercritical fluid extraction of carotenoids and chlorophyll a from Nannochloropsis gaditana. Journal of Food Engineering. 66(2), 245-251. DOI: https://doi.org/10.1016/j.jfoodeng.2004.03.021
  • Stevenson, J. M., Perera, I. Y., Heilmann, I., Persson, S., & Boss, W. F., (2000). Inositol signaling and plant growth. Trends in plant science. 5(6), 252-258. DOI: https://doi.org/10.1016/S1360-1385(00)01652-6
  • Vallurua, R.; Van den Ende, W., (2011). Myo-inositol and beyond – Emerging networks under stress. Plant Science, 181, 387–400. DOI: 10.1016/j.plantsci.2011.07.009.
  • Vismara, R., Vestri, S., Kusmic, C., Barsanti, L., & Gualtieri, P., (2003). Natural vitamin E enrichment of Artemia salina fed freshwater and marine microalgae. Journal of applied phycology, 15(1):75-80. DOI: https://doi.org/10.1023/A:1022942705496
  • Volkman, J. K., Brown, M. R., Dunstan, G. A., & Jeffrey, S. W., (1993). The biochemical composition of marine microalgae from the class Eustigmatophyceae. Journal of Phycology. 29(1), 69-78. DOI: https://doi.org/10.1111/j.1529-8817.1993.tb00281.x
  • Zar, J.H., (1999). Biostatistical Analysis. Upper Saddle River, Prentice Hall, New Jersey, 4th Edition. cap 12, USA, 231-272. https://trove.nla.gov.au/work/7899770
  • Zou, N., & Richmond, A., (2000). Light-path length and population density in photoacclimation of Nannochloropsis sp.(Eustigmatophyceae). Journal of Applied Phycology. 12(3-5), 349-354. DOI: https://doi.org/10.1023/A:1008151004317

Ayrıntılar

Birincil Dil İngilizce
Konular Biyoloji
Bölüm Makaleler
Yazarlar

Yaşar DURMAZ (Sorumlu Yazar)
EGE ÜNİVERSİTESİ
0000-0002-1858-5882
Türkiye


Gökhun Çağatay ERBİL Bu kişi benim
KASTAMONU ÜNİVERSİTESİ
0000-0002-6704-5073
Türkiye

Yayımlanma Tarihi 15 Haziran 2020
Başvuru Tarihi 30 Ekim 2019
Kabul Tarihi 10 Ocak 2020
Yayınlandığı Sayı Yıl 2020, Cilt 37, Sayı 2

Kaynak Göster

APA Durmaz, Y. & Erbil, G. Ç. (2020). Effects of myo-inositol concentration on growth and pigments of Nannochloropsis oculata culture . Ege Journal of Fisheries and Aquatic Sciences , 37 (2) , 195-199 . DOI: 10.12714/egejfas.37.2.11