Extraction of trypsin enzyme from visceral organs of anchovy (Engraulis encrasicolus) and sardine (Sardina pilchardus) and determination of some functional characteristics
Abstract
In this study, nutritional composition analysis of internal organs of anchovy (Engraulis encrasicolus) and sardine (Sardina pilchardus), trypsin activity determination of trypsin enzyme obtained from internal organs, protein solubility and color values of extracted trypsin parameters examined. Nutritional composition of internal organs: the moisture content of the anchovy internal organs 78.92±0.11 %, the ash 2.26±0.55 %, the protein 10.82±2.05 %, the fat 6.49±0.55 % were estimated. The moisture content of the sardine internal organs 81.07±1.03 %, the ash 0.71±0.04 %, the protein 5.08±2.08 %, the fat 2.01±0.17 % were estimated. Enzyme activity and spesicific activity of anchovy trypsin which are 0.064 U/mg, 0.17 U/ml were estimated, respectively. Enzyme activiy of sardine trypsin as 0.051 U/ml and spesific activity as 0.19 U/mg were estimated. In the enzyme purification step, 13.6 g of 1 kg anchovy and 18.9 g of powder trypsin were obtained from 1 kg sardine.
Keywords
Destekleyen Kurum
Proje Numarası
Teşekkür
Kaynakça
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Ayrıntılar
Birincil Dil
Türkçe
Konular
Gıda Mühendisliği
Bölüm
Araştırma Makalesi
Yazarlar
Şükran Çaklı
*
0000-0002-2419-9064
Türkiye
Boğachan Burak Erkan
0000-0002-0924-2116
Türkiye
Yayımlanma Tarihi
15 Mart 2020
Gönderilme Tarihi
12 Temmuz 2019
Kabul Tarihi
1 Ekim 2019
Yayımlandığı Sayı
Yıl 1970 Cilt: 37 Sayı: 1