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Pathogenicity and antibiotic susceptibility of two bacterial pathogens associated with the clam Tapes decussatus in some Egyptian fisheries

Yıl 2017, Cilt: 34 Sayı: 4, 383 - 389, 15.12.2017

Bahaa Abdella , Eman El-wazzan Nermeen El-sersy Soraya Sabry Ehab El-helow

https://doi.org/10.12714/egejfas.2017.34.4.04
Cited By: 4

Öz



Bivalve
aquaculture is generally influenced by bacterial pathogens that cause high
mortality-related losses in hatcheries. Six bacterial phenotypes had been
previously isolated as the most predominant microorganisms in carpet shell clam
(Tapes decussates) samples collected from natural beds in representative
Egyptian clam fisheries. The main aims of this study were to investigate the
nature of the pathogenic strains that significantly affect clam survival and
test their susceptibility to commercially available antibiotics. Based on their
16S rRNA sequences and some biochemical features, two potent clam pathogens
were emerged; one of which is gram-positive and the other is gram-negative. The
former isolate was identified as Micrococcus luteus and the latter as Vibrio
alginolyticus. Experimental challenges with the two bacterial pathogens
introduced at different initial cell concentrations (2.5 X 104 - 7.5
X 104 cfu ml-1) showed markedly diverse clam mortality
results. However, the pathogenic interaction of M. luteus with clam
survival was generally higher than that of V. alginolyticus. When
introduced at a relatively low initial cell density, the infectious records
of V. alginolyticus were significantly increased
suggesting that the expression of its key virulence
factors is mainly triggered as a response to host contact. Antibiotic
susceptibility tests suggested chloramphenicol and tetracycline as markedly
effective agents that can be used to control the spread of these two bacterial
pathogens in aquaculture applications.



Anahtar Kelimeler

Micrococcus luteus Vibrio alginolyticus Tapes decussates pathogenicity fisheries and Aquaculture

Kaynakça

  • Abdella, B., El-Wazzan E., El-Sersy, N.A., El-Helow, E.R. & Sabry, S.A, (2015). Dynamics of bacterial pathogens associated with the clam Tapes decussatus in three main Egyptian fisheries. Journal of Advances in Biology, 8(2):1614-1622.
  • Altuntas, F., Yildiz, O., Eser, B., Gündogan, K., Sumerkan, B. & Çetin, M. (2004). Catheter-related bacteremia due to Kocuria rosea in a patient undergoing peripheral blood stem cell transplantation. BioMedical Central Infectious, 4(1):62. doi: 10.1186/1471-2334-4-62
  • Arapov, J., Ezgeta-Balić, D., Peharda, M. & Ninčević Gladan, Ž. (2010). Bivalve feeding- how and what they eat? Croatian Journal of Fisheries, 68(3):105-116.
  • Aydin, S., Ciltas, A., Yetim, H. & Akyurt, I. (2005). Clinical, pathological and haematological effects of Micrococcus luteus infections in Rainbow Trout (Oncorhynchus mykiss Walbaum). Journal of Animal and Veterinary Advances, 4(2):167-174.
  • Carvalho, E.D., David, G.S. & Silva, R.J. (Eds.). (2012). Health and Environment in Aquaculture. Rijeka, Croatia: InTech. doi: 10.5772/2462
  • Castillo, D., D’Alvise, P., Kalatzis, P. G., Kokkari, C., Middelboe, M., Gram, L., Liu, S. & Katharios, P. (2015). Draft genome sequences of Vibrio alginolyticus strains V1 and V2, opportunistic marine pathogens. Genome Announcements. 3(4):e00729–15. doi: 10.1128/genomeA.00729-15
  • Chatterjee, S. & Haldar, S. (2012). Vibrio related diseases in aquaculture and development of rapid and accurate identification methods. Journal of Marine Science: Research & Development, 1(2):1-7. doi: 10.4172/2155-9910.S1-002
  • Chen, Y., Dai, J., Morris, J.G. Jr. & Johnson, J.A., (2010). Genetic analysis of the capsule polysaccharide (K antigen) and exopolysaccharide genes in pandemic Vibrio parahaemolyticus O3:K6. BMC Microbiology 10:274. doi: 10.1186/1471-2180-10-274
  • CLSI. (2007). Performance Standards for Antimicrobial. Susceptibility Testing; Seventeenth. Informational Supplement. Pennsylvania, USA: Clinical and Laboratory Standards Institute (CLSI).
  • Collin, B., Rehnstam-Holm, A. & Hernroth, B. (2008). Faecal contaminants in edible bivalves from Maputo Bay, Mozambique: Seasonal distribution, pathogenesis and antibiotic resistance. The Open Nutrition Journal, 2:86-93. doi: 10.2174/1874288200802010086
  • Dawood, M. A. & Koshio, S. (2016). Recent advances in the role of probiotics and prebiotics in carp aquaculture: A review. Aquaculture, 454:243-251. doi: 10.1016/j.aquaculture.2015.12.033
  • Deep, A., Chaudhary, U. & Gupta, V., (2011). Quorum sensing and Bacterial Pathogenicity: From Molecules to Disease. Journal of Laboratory Physicians.; 3(1):4-11. doi: 10.4103/0974-2727.78553
  • Deng, L.L., Alexander, A. A., Lei, S. & Anderson, J.S. (2010). The cell wall teichuronic acid synthetase (tuas) is an enzyme complex located in the cytoplasmic membrane of Micrococcus luteus. Biochemistry Research International, 2010:1-8. doi: 10.1155/2010/395758
  • Dubert, J., Romalde, J.L., Prado, S. & Barja, J.L. (2016). Vibrio bivalvicida sp. nov., a novel larval pathogen for bivalve molluscs reared in a hatchery. Systematic and Applied Microbiology, 39(1):8-13. doi: 10.1016/j.syapm.2015.10.006
  • Dubert, J., Aranda-Burgos, J.A., Ojea, J., Barja, J.L. & Prado, S. (2017). Mortality event involving larvae of the carpet shell clam Ruditapes decussatus in a hatchery: isolation of the pathogen Vibrio tubiashii subsp. Europaeus Journal of Fish Diseases 40:1185–1193. doi: 10.1111/jfd.12593
  • Dong, F.M. (Ed.). (2010). The Nutritional Value of Shellfish. Washington, USA: A Washington Sea Grant publication.
  • Duprey, A., Reverchon, S. & Nasser, W. (2014). Bacterial virulence and Fis: adapting regulatory networks to the host environment. Trends in Microbiology, 22(2):92-99. doi: 10.1016/j.tim.2013.11.008
  • Fazlani, S.A., Khan, S.A., Faraz, S. & Awan, M.S. (2011). Antimicrobial susceptibility of bacterial species identified from mastitic milk samples of camel. African Journal of Biotechnology,10(15):2959-2964. doi: 10.5897/AJB10.716
  • Gómez-León, J., Villamil, L., Salger, S. A., Sallum, R.H., Remacha-Triviño, A., Leavitt, D.F. & Gómez-Chiarri, M. (2008). Survival of eastern oysters Crassostrea virginica from three lines following experimental challenge with bacterial pathogens. Diseases of Aquatic Organisms, 79: 95-105. doi: 10.3354/dao01902
  • Hsieh, Y.C., Liang, S.M., Tsai, W.L., Chen, Y.H., Liu, T.Y. & Liang, C.M., (2003). Study of capsular polysaccharide from Vibrio parahaemolyticus. Infection and Immunity, 71: 3329-3336. doi: 10.1128/IAI.71.6.3329-3336.2003
  • Jorgensen, J.H. & Ferraro, M.J. (2009). Antimicrobial susceptibility testing: A review of general principles and contemporary practices. Clinical Infectious Diseases, 49(11):1749-1755. doi: 10.1086/647952
  • Kayser, F.H., Bienz, K.A., Eckert, J. & Zinkernagel, R.M. (Eds.). (2005). Medical Microbiology. New York, USA: Thieme Stuttgart.
  • Kemp, P.S. (Ed.). (2006). Oyster Hatchery Manual. North Carolina, USA: Carteret Community College.
  • Kennedy, D.A., Kurath, G., Brito, I.L., Purcell, M.K., Read, A.F., Winton, J.R. & Wargo, A.R. (2016). Potential drivers of virulence evolution in aquaculture. Evolutionary Applications, 9(2):344-354. doi: 10.1111/eva.12342
  • Kidd, S. P. (Ed.). (2011). Stress Response in Pathogenic Bacteria. Wallingford, Oxfordshire, UK: CAB International. doi: 10.1079/9781845937607.0093
  • Kumar, S., Stecher G. & Tamura, K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33:1870-1874. doi: 10.1093/molbev/msw054
  • Laganà, P., Caruso, G., Minutoli, E., Zaccone, R. & Delia, S. (2011). Susceptibility to antibiotics of Vibrio spp. and Photobacterium damsela ssp. piscicida strains isolated from Italian aquaculture farms. New Microbiologica, 34(1):53-63.
  • Lane, D. (1991). 16S/23S rRNA Sequencing. In Stackebrandt, E. & Goodfellow, M. (Eds.), Nucleic Acid Techniques in Bacterial Systematics (115-175). New York, USA: John Wiley & Sons.
  • Livestrong.com, (1969) 5 Things You Need to Know About the Health Benefits of Clams. Retrieved from LIVESTRONG.COM: http://www.livestrong.com/article/5384-need-health-benefits-clams/ (05.05.2013)
  • Liu, C.H., Cheng, W., Hsu, J.P. & Chen, J.C. (2004). Vibrio alginolyticus infection in the white shrimp Litopenaeus vannamei confirmed by polymerase chain reaction and 16S rDNA sequencing. Diseases of aquatic organisms, 61(1-2):169-174. doi: 10.3354/dao061169
  • Miossec, L., Le Deuff, R.M. & Goulletquer, P. (2009). Alien species alert: Crassostrea gigas (Pacific oyster). ICES Cooperative Research Report, 299.
  • Mustapha, S., Mustapha, E. M. & Nozha, C. (2013). Vibrio alginolyticus: an emerging pathogen of food borne diseases. International Journal of Science and Technology, 2(4):302-309.
  • Ottaviania, D., Bacchiocchi, I., Masini, L., Leoni, F., Carraturo, A., Giammarioli, M. & Sbaraglia, G. (2001). Antimicrobial susceptibility of potentially pathogenic halophilic vibrios isolated from seafood. International Journal of Antimicrobial Agents, 18(2):135-140. doi: 10.1016/S0924-8579(01)00358-2
  • Paillard, C. (2004). A short-review of brown ring disease, a vibrosis affecting clams, Ruditapes philippinarum and Ruditapes deccusatus. Aquatic Living Resources, 17:467-475. doi: 10.1051/alr:2004053
  • Paillard, C., Roux, F. & Borrego, J.J. (2004). Bacterial disease in marine bivalves a review of recent studies: trends and evolution. Aquatic Living Resources, 17:477-498. doi: 10.1051/alr:2004054
  • Park, K., Paillard, C., Le Chevalier, P. & Choi, K. (2006). Report on the occurrence of brown ring disease (BRD) in manila clam, Ruditapes philippinarum on the west coast of Korea. Aquaculture, 255(1-2):610-613. doi: 10.1016/j.aquaculture.2005.12.011
  • PHAC- (2011). Micrococcus spp. Public Health Agency of Canada. Retrieved from http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/micrococcus-eng.php (24.10.2013)
  • Ramos, R.J., Pereira, M.A., Miotto, L.A., Faria, R.D., Junior, N.S. & Vieira, C. R. (2012). Ocurrence of Vibrio spp., positive coagulase staphylococci and enteric bacteria in oysters (Crassostrea gigas) harvested in the south bay of Santa Catarina island, Brazil. Ciência e Tecnologia de Alimentos, 32(3):478-484. doi: 10.1590/S0101-20612012005000061
  • Romalde, J. & Barja, J. (2010). Bacteria in molluscs: good and bad guys. Formatex, 136-147.
  • Romero, J.M., Feijoó, C.G. & Navarrete Wallace, P.A. (2012). Antibiotics in aquaculture use, abuse and alternatives; In: Carvalho, E.D., David, J.S. & Silva, R.J. (Eds.), Health and Environment in Aquaculture, p. 159. doi: 10.5772/28157
  • Rui, H., Liu, Q., Wang, Q., Ma, Y., Liu, H., Shi, C. & Zhang, Y. (2009). Role of alkaline serine protease, asp, in Vibrio alginolyticus virulence and regulation of its expression by luxO-luxR regulatory system. J Microbiol Biotechnol, 19:431-438. doi: 10.4014/jmb.0807.404
  • Ruwandeepika, D., Arachchige, H., Sanjeewa Prasad Jayaweera, T., Paban Bhowmick, P., Karunasagar, I., Bossier, P. & Defoirdt, T. (2012). Pathogenesis, virulence factors and virulence regulation of vibrios belonging to the Harveyi clade. Reviews in Aquaculture, 4(2):59-74. doi: 10.1111/j.1753-5131.2012.01061.x
  • Sandlund, N., Rødseth, O.M., Knappskog, D.H., Fiksdal, I.U. & Bergh, Ø. (2010). Comparative susceptibility of turbot, halibut, and cod yolk-sac larvae to challenge with Vibrio spp. Diseases of Aquatic Organisms, 89: 29-37. doi: 10.3354/dao02176
  • Sifri, C. D. (2008). Quorum Sensing: Bacteria Talk Sense. Clinical Infectious Diseases, 47(8):1070-1076. doi: 10.1086/592072
  • Tubiash, H.S. (1975). Bacterial pathogens associated with cultured bivalve mollusk larvae. In W. L. Smith, & M. H. Chanley (Eds.), Culture of Marine Invertebrate Animals (61-71). New York, USA: Springer. doi: 10.1007/978-1-4615-8714-9_4
  • Wang, Z., Wang, B., Chen, G., Jian, J., Lu, Y., Xu, Y. & Wu, Z. (2016). Transcriptome analysis of the pearl oyster (Pinctada fucata) hemocytes in response to Vibrio alginolyticus infection. Gene 575:421–428. doi: 10.1016/j.gene.2015.09.014
  • Wu, H., Ji, C., Wei, L., Zhao, J. & Lu, H. (2013). Proteomic and metabolomic responses in hepatopancreas of Mytilus galloprovincialis challenged by Micrococcus luteus and Vibrio anguillarum. Journal of Proteomics, 94(6): 54–67. doi: 10.1016/j.jprot.2013.09.001

Yıl 2017, Cilt: 34 Sayı: 4, 383 - 389, 15.12.2017

Bahaa Abdella , Eman El-wazzan Nermeen El-sersy Soraya Sabry Ehab El-helow

https://doi.org/10.12714/egejfas.2017.34.4.04
Cited By: 4

Öz


Kaynakça

  • Abdella, B., El-Wazzan E., El-Sersy, N.A., El-Helow, E.R. & Sabry, S.A, (2015). Dynamics of bacterial pathogens associated with the clam Tapes decussatus in three main Egyptian fisheries. Journal of Advances in Biology, 8(2):1614-1622.
  • Altuntas, F., Yildiz, O., Eser, B., Gündogan, K., Sumerkan, B. & Çetin, M. (2004). Catheter-related bacteremia due to Kocuria rosea in a patient undergoing peripheral blood stem cell transplantation. BioMedical Central Infectious, 4(1):62. doi: 10.1186/1471-2334-4-62
  • Arapov, J., Ezgeta-Balić, D., Peharda, M. & Ninčević Gladan, Ž. (2010). Bivalve feeding- how and what they eat? Croatian Journal of Fisheries, 68(3):105-116.
  • Aydin, S., Ciltas, A., Yetim, H. & Akyurt, I. (2005). Clinical, pathological and haematological effects of Micrococcus luteus infections in Rainbow Trout (Oncorhynchus mykiss Walbaum). Journal of Animal and Veterinary Advances, 4(2):167-174.
  • Carvalho, E.D., David, G.S. & Silva, R.J. (Eds.). (2012). Health and Environment in Aquaculture. Rijeka, Croatia: InTech. doi: 10.5772/2462
  • Castillo, D., D’Alvise, P., Kalatzis, P. G., Kokkari, C., Middelboe, M., Gram, L., Liu, S. & Katharios, P. (2015). Draft genome sequences of Vibrio alginolyticus strains V1 and V2, opportunistic marine pathogens. Genome Announcements. 3(4):e00729–15. doi: 10.1128/genomeA.00729-15
  • Chatterjee, S. & Haldar, S. (2012). Vibrio related diseases in aquaculture and development of rapid and accurate identification methods. Journal of Marine Science: Research & Development, 1(2):1-7. doi: 10.4172/2155-9910.S1-002
  • Chen, Y., Dai, J., Morris, J.G. Jr. & Johnson, J.A., (2010). Genetic analysis of the capsule polysaccharide (K antigen) and exopolysaccharide genes in pandemic Vibrio parahaemolyticus O3:K6. BMC Microbiology 10:274. doi: 10.1186/1471-2180-10-274
  • CLSI. (2007). Performance Standards for Antimicrobial. Susceptibility Testing; Seventeenth. Informational Supplement. Pennsylvania, USA: Clinical and Laboratory Standards Institute (CLSI).
  • Collin, B., Rehnstam-Holm, A. & Hernroth, B. (2008). Faecal contaminants in edible bivalves from Maputo Bay, Mozambique: Seasonal distribution, pathogenesis and antibiotic resistance. The Open Nutrition Journal, 2:86-93. doi: 10.2174/1874288200802010086
  • Dawood, M. A. & Koshio, S. (2016). Recent advances in the role of probiotics and prebiotics in carp aquaculture: A review. Aquaculture, 454:243-251. doi: 10.1016/j.aquaculture.2015.12.033
  • Deep, A., Chaudhary, U. & Gupta, V., (2011). Quorum sensing and Bacterial Pathogenicity: From Molecules to Disease. Journal of Laboratory Physicians.; 3(1):4-11. doi: 10.4103/0974-2727.78553
  • Deng, L.L., Alexander, A. A., Lei, S. & Anderson, J.S. (2010). The cell wall teichuronic acid synthetase (tuas) is an enzyme complex located in the cytoplasmic membrane of Micrococcus luteus. Biochemistry Research International, 2010:1-8. doi: 10.1155/2010/395758
  • Dubert, J., Romalde, J.L., Prado, S. & Barja, J.L. (2016). Vibrio bivalvicida sp. nov., a novel larval pathogen for bivalve molluscs reared in a hatchery. Systematic and Applied Microbiology, 39(1):8-13. doi: 10.1016/j.syapm.2015.10.006
  • Dubert, J., Aranda-Burgos, J.A., Ojea, J., Barja, J.L. & Prado, S. (2017). Mortality event involving larvae of the carpet shell clam Ruditapes decussatus in a hatchery: isolation of the pathogen Vibrio tubiashii subsp. Europaeus Journal of Fish Diseases 40:1185–1193. doi: 10.1111/jfd.12593
  • Dong, F.M. (Ed.). (2010). The Nutritional Value of Shellfish. Washington, USA: A Washington Sea Grant publication.
  • Duprey, A., Reverchon, S. & Nasser, W. (2014). Bacterial virulence and Fis: adapting regulatory networks to the host environment. Trends in Microbiology, 22(2):92-99. doi: 10.1016/j.tim.2013.11.008
  • Fazlani, S.A., Khan, S.A., Faraz, S. & Awan, M.S. (2011). Antimicrobial susceptibility of bacterial species identified from mastitic milk samples of camel. African Journal of Biotechnology,10(15):2959-2964. doi: 10.5897/AJB10.716
  • Gómez-León, J., Villamil, L., Salger, S. A., Sallum, R.H., Remacha-Triviño, A., Leavitt, D.F. & Gómez-Chiarri, M. (2008). Survival of eastern oysters Crassostrea virginica from three lines following experimental challenge with bacterial pathogens. Diseases of Aquatic Organisms, 79: 95-105. doi: 10.3354/dao01902
  • Hsieh, Y.C., Liang, S.M., Tsai, W.L., Chen, Y.H., Liu, T.Y. & Liang, C.M., (2003). Study of capsular polysaccharide from Vibrio parahaemolyticus. Infection and Immunity, 71: 3329-3336. doi: 10.1128/IAI.71.6.3329-3336.2003
  • Jorgensen, J.H. & Ferraro, M.J. (2009). Antimicrobial susceptibility testing: A review of general principles and contemporary practices. Clinical Infectious Diseases, 49(11):1749-1755. doi: 10.1086/647952
  • Kayser, F.H., Bienz, K.A., Eckert, J. & Zinkernagel, R.M. (Eds.). (2005). Medical Microbiology. New York, USA: Thieme Stuttgart.
  • Kemp, P.S. (Ed.). (2006). Oyster Hatchery Manual. North Carolina, USA: Carteret Community College.
  • Kennedy, D.A., Kurath, G., Brito, I.L., Purcell, M.K., Read, A.F., Winton, J.R. & Wargo, A.R. (2016). Potential drivers of virulence evolution in aquaculture. Evolutionary Applications, 9(2):344-354. doi: 10.1111/eva.12342
  • Kidd, S. P. (Ed.). (2011). Stress Response in Pathogenic Bacteria. Wallingford, Oxfordshire, UK: CAB International. doi: 10.1079/9781845937607.0093
  • Kumar, S., Stecher G. & Tamura, K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33:1870-1874. doi: 10.1093/molbev/msw054
  • Laganà, P., Caruso, G., Minutoli, E., Zaccone, R. & Delia, S. (2011). Susceptibility to antibiotics of Vibrio spp. and Photobacterium damsela ssp. piscicida strains isolated from Italian aquaculture farms. New Microbiologica, 34(1):53-63.
  • Lane, D. (1991). 16S/23S rRNA Sequencing. In Stackebrandt, E. & Goodfellow, M. (Eds.), Nucleic Acid Techniques in Bacterial Systematics (115-175). New York, USA: John Wiley & Sons.
  • Livestrong.com, (1969) 5 Things You Need to Know About the Health Benefits of Clams. Retrieved from LIVESTRONG.COM: http://www.livestrong.com/article/5384-need-health-benefits-clams/ (05.05.2013)
  • Liu, C.H., Cheng, W., Hsu, J.P. & Chen, J.C. (2004). Vibrio alginolyticus infection in the white shrimp Litopenaeus vannamei confirmed by polymerase chain reaction and 16S rDNA sequencing. Diseases of aquatic organisms, 61(1-2):169-174. doi: 10.3354/dao061169
  • Miossec, L., Le Deuff, R.M. & Goulletquer, P. (2009). Alien species alert: Crassostrea gigas (Pacific oyster). ICES Cooperative Research Report, 299.
  • Mustapha, S., Mustapha, E. M. & Nozha, C. (2013). Vibrio alginolyticus: an emerging pathogen of food borne diseases. International Journal of Science and Technology, 2(4):302-309.
  • Ottaviania, D., Bacchiocchi, I., Masini, L., Leoni, F., Carraturo, A., Giammarioli, M. & Sbaraglia, G. (2001). Antimicrobial susceptibility of potentially pathogenic halophilic vibrios isolated from seafood. International Journal of Antimicrobial Agents, 18(2):135-140. doi: 10.1016/S0924-8579(01)00358-2
  • Paillard, C. (2004). A short-review of brown ring disease, a vibrosis affecting clams, Ruditapes philippinarum and Ruditapes deccusatus. Aquatic Living Resources, 17:467-475. doi: 10.1051/alr:2004053
  • Paillard, C., Roux, F. & Borrego, J.J. (2004). Bacterial disease in marine bivalves a review of recent studies: trends and evolution. Aquatic Living Resources, 17:477-498. doi: 10.1051/alr:2004054
  • Park, K., Paillard, C., Le Chevalier, P. & Choi, K. (2006). Report on the occurrence of brown ring disease (BRD) in manila clam, Ruditapes philippinarum on the west coast of Korea. Aquaculture, 255(1-2):610-613. doi: 10.1016/j.aquaculture.2005.12.011
  • PHAC- (2011). Micrococcus spp. Public Health Agency of Canada. Retrieved from http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/micrococcus-eng.php (24.10.2013)
  • Ramos, R.J., Pereira, M.A., Miotto, L.A., Faria, R.D., Junior, N.S. & Vieira, C. R. (2012). Ocurrence of Vibrio spp., positive coagulase staphylococci and enteric bacteria in oysters (Crassostrea gigas) harvested in the south bay of Santa Catarina island, Brazil. Ciência e Tecnologia de Alimentos, 32(3):478-484. doi: 10.1590/S0101-20612012005000061
  • Romalde, J. & Barja, J. (2010). Bacteria in molluscs: good and bad guys. Formatex, 136-147.
  • Romero, J.M., Feijoó, C.G. & Navarrete Wallace, P.A. (2012). Antibiotics in aquaculture use, abuse and alternatives; In: Carvalho, E.D., David, J.S. & Silva, R.J. (Eds.), Health and Environment in Aquaculture, p. 159. doi: 10.5772/28157
  • Rui, H., Liu, Q., Wang, Q., Ma, Y., Liu, H., Shi, C. & Zhang, Y. (2009). Role of alkaline serine protease, asp, in Vibrio alginolyticus virulence and regulation of its expression by luxO-luxR regulatory system. J Microbiol Biotechnol, 19:431-438. doi: 10.4014/jmb.0807.404
  • Ruwandeepika, D., Arachchige, H., Sanjeewa Prasad Jayaweera, T., Paban Bhowmick, P., Karunasagar, I., Bossier, P. & Defoirdt, T. (2012). Pathogenesis, virulence factors and virulence regulation of vibrios belonging to the Harveyi clade. Reviews in Aquaculture, 4(2):59-74. doi: 10.1111/j.1753-5131.2012.01061.x
  • Sandlund, N., Rødseth, O.M., Knappskog, D.H., Fiksdal, I.U. & Bergh, Ø. (2010). Comparative susceptibility of turbot, halibut, and cod yolk-sac larvae to challenge with Vibrio spp. Diseases of Aquatic Organisms, 89: 29-37. doi: 10.3354/dao02176
  • Sifri, C. D. (2008). Quorum Sensing: Bacteria Talk Sense. Clinical Infectious Diseases, 47(8):1070-1076. doi: 10.1086/592072
  • Tubiash, H.S. (1975). Bacterial pathogens associated with cultured bivalve mollusk larvae. In W. L. Smith, & M. H. Chanley (Eds.), Culture of Marine Invertebrate Animals (61-71). New York, USA: Springer. doi: 10.1007/978-1-4615-8714-9_4
  • Wang, Z., Wang, B., Chen, G., Jian, J., Lu, Y., Xu, Y. & Wu, Z. (2016). Transcriptome analysis of the pearl oyster (Pinctada fucata) hemocytes in response to Vibrio alginolyticus infection. Gene 575:421–428. doi: 10.1016/j.gene.2015.09.014
  • Wu, H., Ji, C., Wei, L., Zhao, J. & Lu, H. (2013). Proteomic and metabolomic responses in hepatopancreas of Mytilus galloprovincialis challenged by Micrococcus luteus and Vibrio anguillarum. Journal of Proteomics, 94(6): 54–67. doi: 10.1016/j.jprot.2013.09.001
Toplam 47 adet kaynakça vardır.

Ayrıntılar

Bölüm Makaleler
Yazarlar

Bahaa Abdella 0000-0001-5237-263X

Eman El-wazzan 0000-0001-8615-5588

Nermeen El-sersy 0000-0001-8724-0303

Soraya Sabry 0000-0001-7428-4665

Ehab El-helow 0000-0002-4319-3856

Yayımlanma Tarihi 15 Aralık 2017
Gönderilme Tarihi 30 Haziran 2017
Yayımlandığı Sayı Yıl 2017Cilt: 34 Sayı: 4

Kaynak Göster

APA Abdella, B., El-wazzan, E., El-sersy, N., Sabry, S., vd. (2017). Pathogenicity and antibiotic susceptibility of two bacterial pathogens associated with the clam Tapes decussatus in some Egyptian fisheries. Ege Journal of Fisheries and Aquatic Sciences, 34(4), 383-389. https://doi.org/10.12714/egejfas.2017.34.4.04

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