Effect of different protein sources on protease activity of northern pike, Esox lucius Linneaus 1758, juvenile

Cemil Kaya Gökçek; Tamás Szabó; Cüneyt Suzer

doi:10.12714/egejfas.37.2.03

TR EN

Farklı protein kaynaklarının turna, Esox lucius Linneaus 1758, jüvenillerinin proteaz aktivitesi üzerine etkisi

Öz

Bu çalışmanın amacı, farklı protein kaynaklarının turna balığının larval gelişimi sırasında sindirim enzimlerinden proteaz enzimlerini inhibe edici etkisinin tespit edilmesidir. Bu amaçla, Turna balığı, E. lucius, larvaları yumurtadan çıkıştan itibaren 21. günün sonuna kadar beslenmişlerdir. Bu noktada, besleme yapılan larvalardan 7, 14 ve 21. günlerde örnekler alınarak in vitro enzim aktiviteleri tespit edilmiştir. Çalışmada, protein kaynağı olarak iki farklı balık unu, tavuk unu, krill unu, mısır glüteni, soya protein konsantresi, soya unu ve damıtılmış tahıl test edilmiştir. Yedinci günde, en düşük etki balık unu-I grubunda tespit edilmiştir (% 7,53). Ayrıca, tavuk unu ilk hafta en yüksek durdurucu etkiyi göstermiştir (% 68,27). Bunu takip eden dönemde (14. gün), tüm hammaddelerin durdurucu etkisi dramatik bir şekilde artmış olsa da, proteolitik aktivite üzerindeki en düşük etki yine balık unu-I grubunda görülmüştür (% 55,66). Aynı dönem içerisinde, en yüksek etki krill ve tavuk ununda tespit edilmiştir (sırasıyla, %82,28 ve 86,73). Ardından, 21. günde ise balık unu-I, balık unu-II ve mısır gluteni arasında herhangi bir istatistiki fark bulunmamıştır (p>0.05). Bunun yanında, tavuk unu bu dönemde de enzim aktivitesini durdurucu en yüksek etkiye sahip olmuştur (% 89,27). Sonuç olarak, 7- 14 ve 21. günlerde proteolitik aktivitenin belirgin artış göstermesi ile birlikte, bu türden karnivor balıkların larva ve juvenil boylarının beslenmesi sırasında canlı yemlerin kullanılmasının daha başarılı gelişim sonuçlarını ortaya koyacağı düşünülmektedir.

Anahtar Kelimeler

Effect of different protein sources on protease activity of northern pike, Esox lucius Linneaus 1758, juvenile

Öz

The aim of the study is to determine the inhibitory effect of different protein sources on protease activity of Northern pike, E. lucius, during larval ontogeny. For this purpose, Northern pike were fed from yolk sac absorption until 21 days after hatching (DAH). At that point, larvae were sampled on 7, 14 and 21 DAH days and the activity of enzyme was analyzed in vitro. In the study, two different fish meal, chicken meal, krill meal, corn gluten, soybean protein concentrate, soybean meal and dried distillers grains with solubles were tested. Fish meal-I showed the lowest effect (7.53 %) on 7 DAH larvae. Moreover, chicken meal has the highest inhibitory effect on the proteases in the first week (68.27%). In the following period (DAH 14), although the inhibition ratio dramatically increased in all ingredients, fish meal-I has still the lowest effect on proteolytic activity (55.66%). In the same period, the highest effect was obtained from krill (82.28 %) and chicken meals (86.73 %), respectively. Then, there was no statistical difference between fish meal-I, fish meal-II and corn gluten in the 21 DAH and relatively lower than the others (p>0.05). Additionally, chicken meal again has the highest effect on juveniles with the ratio 89.27 %. As a result, the increase of proteolytic activity was notably increased in 7-14-21 DAH, however, it is concluded that feeding larvae and juveniles with live prey is still suggested to get better result for such a carnivorous species culture.

Anahtar Kelimeler

Destekleyen Kurum

Hatay Mustafa Kemal University Scientific Projects Department

Proje Numarası

16792

Teşekkür

Special thanks to Francisco Javier MOYANO for sharing enzyme analyze methods and for future project ideas. The present work was supported from Mustafa Kemal University Scientific Projects Department with the project number 16792

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Cemil Kaya Gökçek ^*
0000-0002-7837-8009
Türkiye

Tamás Szabó
0000-0002-3843-4811
Hungary

Cüneyt Suzer
0000-0003-3535-2236
Türkiye

Yayımlanma Tarihi

15 Haziran 2020

Gönderilme Tarihi

30 Ekim 2019

Kabul Tarihi

29 Kasım 2019

Yayımlandığı Sayı

Yıl 2020 Cilt: 37 Sayı: 2

DOI

https://doi.org/10.12714/egejfas.37.2.03

IZ

https://izlik.org/JA82UH79PR

Kaynak Göster

RIS / Bibtex

APA

Gökçek, C. K., Szabó, T., & Suzer, C. (2020). Effect of different protein sources on protease activity of northern pike, Esox lucius Linneaus 1758, juvenile. Ege Journal of Fisheries and Aquatic Sciences, 37(2), 135-138. https://doi.org/10.12714/egejfas.37.2.03

AMA

1.Gökçek CK, Szabó T, Suzer C. Effect of different protein sources on protease activity of northern pike, Esox lucius Linneaus 1758, juvenile. EgeJFAS. 2020;37(2):135-138. doi:10.12714/egejfas.37.2.03

Chicago

Gökçek, Cemil Kaya, Tamás Szabó, ve Cüneyt Suzer. 2020. “Effect of different protein sources on protease activity of northern pike, Esox lucius Linneaus 1758, juvenile”. Ege Journal of Fisheries and Aquatic Sciences 37 (2): 135-38. https://doi.org/10.12714/egejfas.37.2.03.

EndNote

Gökçek CK, Szabó T, Suzer C (01 Haziran 2020) Effect of different protein sources on protease activity of northern pike, Esox lucius Linneaus 1758, juvenile. Ege Journal of Fisheries and Aquatic Sciences 37 2 135–138.

IEEE

[1]C. K. Gökçek, T. Szabó, ve C. Suzer, “Effect of different protein sources on protease activity of northern pike, Esox lucius Linneaus 1758, juvenile”, EgeJFAS, c. 37, sy 2, ss. 135–138, Haz. 2020, doi: 10.12714/egejfas.37.2.03.

ISNAD

Gökçek, Cemil Kaya - Szabó, Tamás - Suzer, Cüneyt. “Effect of different protein sources on protease activity of northern pike, Esox lucius Linneaus 1758, juvenile”. Ege Journal of Fisheries and Aquatic Sciences 37/2 (01 Haziran 2020): 135-138. https://doi.org/10.12714/egejfas.37.2.03.

JAMA

1.Gökçek CK, Szabó T, Suzer C. Effect of different protein sources on protease activity of northern pike, Esox lucius Linneaus 1758, juvenile. EgeJFAS. 2020;37:135–138.

MLA

Gökçek, Cemil Kaya, vd. “Effect of different protein sources on protease activity of northern pike, Esox lucius Linneaus 1758, juvenile”. Ege Journal of Fisheries and Aquatic Sciences, c. 37, sy 2, Haziran 2020, ss. 135-8, doi:10.12714/egejfas.37.2.03.

Vancouver

1.Cemil Kaya Gökçek, Tamás Szabó, Cüneyt Suzer. Effect of different protein sources on protease activity of northern pike, Esox lucius Linneaus 1758, juvenile. EgeJFAS. 01 Haziran 2020;37(2):135-8. doi:10.12714/egejfas.37.2.03