Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb

Figen Esin Kayhan; Güllü Kaymak; Harika Eylül Esmer Duruel; Şeyma Kızılkaya

doi:10.12714/egejfas.36.4.02

TR EN

Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb

Öz

The potential toxic effects of mancozeb exposure on some antioxidant enzyme were investigated on heart tissue of zebrafish in this study. Zebrafish groups were exposed to different doses of mancozeb (Group A: 5 mgL^-1 and Group B: 7.5 mgL^-1) for 120 hours. In this study, catalase (CAT) activity, malondialdehyde (MDA) level and total protein (TP) level were determined with spectrophotometer. Our results showed that CAT activity was found 2,541±0,771 mg L^-1 in A group and 2,011±0,201 mg L^-1 in B group in this study. CAT and MDA activity levels decreased in the experiment group according to control group. MDA levels were found 0,025±0,003 mg L^-1 in A group and 0,025±0,003 mg L^-1 in B group. TP levels were found 9,75±1,51 mg L^-1 in A group and 10,18±0,32 mg L^-1 in B group. TP levels increased in the all experiment groups according to control group. We observed that the changes in the CAT activity and MDA levels were time and as well as mancozeb dose dependent. As a result mancozeb is a very toxic substance for zebrafish and other aquatic organisms.

Anahtar Kelimeler

Mancozeb,oxidative stress,catalase,malondialdehyde,zebrafish

Kaynakça

Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105,121-126. DOI: 10.1016/S0076-6879(84)05016-3
Andreu-Sanchez, O., Paraiba, L.C., Jonsson, C.M., Carrasco, J.M. (2012). Acute toxicity and bioconcentration of fungicide tebuconazole in zebrafish (Danio rerio). Environmental Toxicology, 27:155-168. DOI:10.1002/tox.20618
Blahova, J., Plhalova, L., Hostovsky, M., Divisova, L., Dobsikova, R., Mikulikova, I., Stepanova, S. & Z. Svobodova. (2013). Oxidative stress responses in zebrafish Danio rerio after subchronic exposure to atrazine. Food and Chemical Toxicology, 61, 82-85. DOI: 10.1016/j.fct.2013.02.041
Bradford, M.M. (1976). A Rapid and Sensitive Method for the Quantitation of Microgram Quantitites of Protein Utilizing the Principle of Protein-dye Binding. Analytical Biochemistry, 72, 248-254. DOI: 10.1016/0003-2697(76)90527-3
Clasen, B., Loro, V.L., Murussi, C.R., Tiecher, T.L., Moraes, B., Zanella, R. (2018). Bioaccumulation and oxidative stress caused by pesticides in Cyprinus carpio reared in a rice-fish system. Science of the Total Environment, 626:737-743.
EPA (US Environmental Protection Agency) (2005). Mancozeb facts, EPA 738-F-05-XX, (http://www3.epa.gov/pesticides/chem_search/reg_actions/reregistration/fs_PC-014504_1-Sep-05.pdf).
Faggio, C., Pagano, M., Alampi, R., Vazzana, I. & Felice, MR. (2016). Cytotoxicity, haemolymphatic parameters, and oxidative stress following exposure to sublethal concentrations of quaternium-15 in Mytilus galloprovincialis. Aquatic Toxicology, 180: 258-265. DOI: 10.1016/j.aquatox.2016.10.010
Karaca, M., Varışlı, L., Korkmaz, K., Özaydın, O., perçin, F., Orhan, H. (2014). Organochlorine pesticides and antioxidant enzymes are inversely correlated with liver enzyme gene expression in Cyrinus carpio. Toxicology Letters, 230:198-207. DOI: 10.1016/j.toxlet.2014.02.013

Konyalıoğlu, S and Perçin, F. (2017). The comparison of lipid peroxidation, glutathione land antioxidant enzyme activities in blood obtained from captive and wild Northern Bluefin Tuna (Thunnus thynnus L., 1758). Free Radical Biology and Medicine, 108:S18-S107. DOI: 10.1016/j.freeradbiomed.2017.04.196
Kumar, S., Nehra, M., Dilbaghi, N., Marrazza, G., Hassan, A.A. & Kim, KH. (2019). Nano-based smart pesticide formulations: Emerging opportunities for agriculture. Journal of Controlled Release, 294,131-153. DOI: 10.1016/j.jconrel.2018.12.012
Ledwozyw, A., Michalak, D., Stepien, A. & A. Kadziolka. (1986). The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis. Clinica Chimica Acta, 155, 275- 83. DOI: 10.1016/0009-8981(86)90247-0
Maharajan, K., Muthulakshmi, S., Nataraj, B., Ramesh, M. (2018). Toxicity assessment of pyriproxyfen in vertebrate model zebrafish embryos (Danio rerio): A multi biomarker study. Aquatic Toxicology, 196:132-145. DOI: 10.1016/j.aquatox.2018.01.010
Mc Collum, C.W., Ducharme, N.A., Bondesson, M., Gustafsson, J.A. (2011). Developmental toxicity Screening in zebrafish. Birth Defects Research. Part-C. Embryo Today Reviews, 93:67-114. DOI: 10.1002/bdrc.20210
Miron, D.S., Prettob, A., Crestani, M., Glusczak, L., Schetinger, M.R., Loro, V.L. & Morsch, V.M. (2008). Biochemical effects of clomazone herbicide on piava (L. obtusidens). Chemosphere, 74, 1–5. DOI: 10.1016/j.chemosphere.2008.09.070
Moraes, B.S., Clasen, B., Loro, V.L., Pretto, A., Toni, C., De Avila, L.A., Marchesan, E., De Oliveira Machado, S.L., Zanella, R. & G.B. Reimche. (2011). Toxicological responses of Cyprinus carpio after exposure to a commercial herbicide containing imazethapyr and imazapic. Ecotoxicology and Environmental Safety, 74, 328-335. DOI: 10.1016/j.ecoenv.2009.05.013
Morales, A.E., Pèrez-Jimènez, A., Hidalgo, M.C., Abellán, E. & Gabriel C.G. (2004). Oxidative stress and antioxidant defenses after prolonged starvation in Dentex dentex liver. Comporative Biochemistry and Physiology C, 139(1-3),153-161. DOI: 10.1016/j.cca.2004.10.008
Pascual, P., Pedrajas, J., Toribio, F., López-Barea, J., & Peinado, J. (2003). Effect of food deprivation on oxidative stress biomarkers in fish (Sparus aurata). Chemico-Biological Interactions, 145(2), 191-199. DOI: 10.1016/s0009-2797(03)00002-4
Percin, F., Sogut, O. (2010). Trace elements in heart tissue of wild and fattened bluefin tuna (Thunnus thynnus L. 1758) in the Turkish part of the Eastern Mediterranean. Journal of Food Agriculture and Environment. 8 (3) 1184-1187.
Plhalova, L., Blahova, J., Divisova, L., Enevova, V., Casuscelli di Tocco, F., Faggio, C., Tichy, F., Vecerek, V. & Z. Svobodova. (2017): The effects of subchronic exposure to NeemAzal T/S on zebrafish (Danio rerio), Chemistry and Ecology, DOI: 10.1080/02757540.2017.1420176
Qiu, L., Jia, K., Huang, L., Liano, X., Guo, X., Lu, H. (2019). Hepatotoxicity of tricylazole in zebrafish (Danio rerio). Chemosphere, 232:171-179. DOI: 10.1016/j.chemosphere.2019.05.159
Ribas, L. and Pifeffer, F. (2013). The zebrafish (Danio rerio) as a model organism, with emphasis on applications for finfish aquaculture research. Reviews in Aquaculture, 5:1-32. DOI: 10.1111/raq.12041
San, A.T. & Yonar, M.E. (2017). Determination of oxidative stress in scaly carp (Cyprinus carpio Linnaeus, 1758) exposed to deltamethrin in different water temperature. Ege Journal of Fisheries and Aquatic Sciences, 34(3), 281-286. DOI: 10.12714/egejfas.2017.34.3.06
Tabassum, H., Dawood, A.Q., Sharma, P., Khan, J., Raisuddin, S. & S. Parvez. (2016). Multiorgan toxicological impact of fungicide propiconazole on biochemical and histological profile of freshwater fish Channa punctata Bloch. Ecological Indicators, 63, 359-365. DOI: 10.1016/j.ecolind.2015.11.052
Tsaboula, A., Papadakis, E.N., Vyrzas, Z., Kotopoulou, A., Kintzikoglou, K. & E. Papadopoulou-Mourkidou. (2016). Environmental and human risk hierarchy of pesticides: A prioritization method, based on monitoring, hazard assessment and environmental fate. Environment International, 91, 78-93. DOI: 10.1016/j.envint.2016.02.008
Vieira, C.E.D., Perez, M.R., Acayaba, R.D., Raimundo, C.C.M., Martinez, C.B.R. (2018). DNA damage and oxidative stress induced by imidacloprid exposure in different tissues of the Neotropical fish Prochilodus lineatus. Chemosphere, 195:125-134. DOI: 10.1016/j.chemosphere.2017.12.077
Wagner, D., Brühl, M., Veith, P.P. & S. Lötters. (2014). Evaluating the risk of pesticide exposure for amphibian species listed in Annex II of the European Union Habitats Directive. Biological Conservation, 176, 64–70. DOI: 10.1016/j.biocon.2014.05.014
Zengin, H. (2018). Oxidative stress and antioxidant defenses after starvation in Oncorhynchus mykiss (Walbaum,1792) larvae reached free swimming stage. Ege Journal of Fisheries and Aquatic Sciences, 35(4), 387-396. DOI: 10.12714/egejfas.2018.35.4.04
Zhu, X.Y., Xia, B., Wu, Y.Y., Yang, H., Li, C.Q., Li, P. (2019). Fenobucarb induces heart failure and cerebral hemorrhage in zebrafish. Aquatic Toxicology, 209:34-41. DOI: 10.1016/j.aquatox.2018.12.020

Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Figen Esin Kayhan ^*
0000-0001-7754-1356
Türkiye

Güllü Kaymak
0000-0001-6309-0208
Türkiye

Harika Eylül Esmer Duruel
0000-0002-0792-2062

Şeyma Kızılkaya
0000-0001-8065-217X
Türkiye

Yayımlanma Tarihi

15 Aralık 2019

Gönderilme Tarihi

5 Şubat 2019

Kabul Tarihi

2 Temmuz 2019

Yayımlandığı Sayı

Yıl 2019 Cilt: 36 Sayı: 4

DOI

https://doi.org/10.12714/egejfas.36.4.02

IZ

https://izlik.org/JA25AU96UC

Kaynak Göster

RIS / Bibtex

APA

Kayhan, F. E., Kaymak, G., Esmer Duruel, H. E., & Kızılkaya, Ş. (2019). Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb. Ege Journal of Fisheries and Aquatic Sciences, 36(4), 325-328. https://doi.org/10.12714/egejfas.36.4.02

AMA

1.Kayhan FE, Kaymak G, Esmer Duruel HE, Kızılkaya Ş. Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb. EgeJFAS. 2019;36(4):325-328. doi:10.12714/egejfas.36.4.02

Chicago

Kayhan, Figen Esin, Güllü Kaymak, Harika Eylül Esmer Duruel, ve Şeyma Kızılkaya. 2019. “Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb”. Ege Journal of Fisheries and Aquatic Sciences 36 (4): 325-28. https://doi.org/10.12714/egejfas.36.4.02.

EndNote

Kayhan FE, Kaymak G, Esmer Duruel HE, Kızılkaya Ş (01 Aralık 2019) Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb. Ege Journal of Fisheries and Aquatic Sciences 36 4 325–328.

IEEE

[1]F. E. Kayhan, G. Kaymak, H. E. Esmer Duruel, ve Ş. Kızılkaya, “Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb”, EgeJFAS, c. 36, sy 4, ss. 325–328, Ara. 2019, doi: 10.12714/egejfas.36.4.02.

ISNAD

Kayhan, Figen Esin - Kaymak, Güllü - Esmer Duruel, Harika Eylül - Kızılkaya, Şeyma. “Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb”. Ege Journal of Fisheries and Aquatic Sciences 36/4 (01 Aralık 2019): 325-328. https://doi.org/10.12714/egejfas.36.4.02.

JAMA

1.Kayhan FE, Kaymak G, Esmer Duruel HE, Kızılkaya Ş. Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb. EgeJFAS. 2019;36:325–328.

MLA

Kayhan, Figen Esin, vd. “Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb”. Ege Journal of Fisheries and Aquatic Sciences, c. 36, sy 4, Aralık 2019, ss. 325-8, doi:10.12714/egejfas.36.4.02.

Vancouver

1.Figen Esin Kayhan, Güllü Kaymak, Harika Eylül Esmer Duruel, Şeyma Kızılkaya. Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb. EgeJFAS. 01 Aralık 2019;36(4):325-8. doi:10.12714/egejfas.36.4.02

Zebrabalığı (Danio reiro) kalp dokusunda mancozeb’in neden olduğu bazı oksidatif stres parametreleri

Öz

Anahtar Kelimeler

Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb

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