Araştırma Makalesi

Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb

Cilt: 36 Sayı: 4 15 Aralık 2019
PDF İndir
TR EN

Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb

Abstract



The potential toxic effects of mancozeb exposure on some antioxidant enzyme were investigated on heart tissue of zebrafish in this study. Zebrafish groups were exposed to different doses of mancozeb (Group A: 5 mgL-1 and Group B: 7.5 mgL-1) for 120 hours. In this study, catalase (CAT) activity, malondialdehyde (MDA) level and total protein (TP) level were determined with spectrophotometer. Our results showed that CAT activity was found 2,541±0,771 mg L-1 in A group and 2,011±0,201 mg L-1 in B group in this study. CAT and MDA activity levels decreased in the experiment group according to control group. MDA levels were found 0,025±0,003 mg L-1 in A group and 0,025±0,003 mg L-1 in B group. TP levels were found 9,75±1,51 mg L-1 in A group and 10,18±0,32 mg L-1 in B group. TP levels increased in the all experiment groups according to control group. We observed that the changes in the CAT activity and MDA levels were time and as well as mancozeb dose dependent. As a result mancozeb is a very toxic substance for zebrafish and other aquatic organisms.   




Keywords

Kaynakça

  1. Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105,121-126. DOI: 10.1016/S0076-6879(84)05016-3
  2. Andreu-Sanchez, O., Paraiba, L.C., Jonsson, C.M., Carrasco, J.M. (2012). Acute toxicity and bioconcentration of fungicide tebuconazole in zebrafish (Danio rerio). Environmental Toxicology, 27:155-168. DOI:10.1002/tox.20618
  3. Blahova, J., Plhalova, L., Hostovsky, M., Divisova, L., Dobsikova, R., Mikulikova, I., Stepanova, S. & Z. Svobodova. (2013). Oxidative stress responses in zebrafish Danio rerio after subchronic exposure to atrazine. Food and Chemical Toxicology, 61, 82-85. DOI: 10.1016/j.fct.2013.02.041
  4. Bradford, M.M. (1976). A Rapid and Sensitive Method for the Quantitation of Microgram Quantitites of Protein Utilizing the Principle of Protein-dye Binding. Analytical Biochemistry, 72, 248-254. DOI: 10.1016/0003-2697(76)90527-3
  5. Clasen, B., Loro, V.L., Murussi, C.R., Tiecher, T.L., Moraes, B., Zanella, R. (2018). Bioaccumulation and oxidative stress caused by pesticides in Cyprinus carpio reared in a rice-fish system. Science of the Total Environment, 626:737-743.
  6. EPA (US Environmental Protection Agency) (2005). Mancozeb facts, EPA 738-F-05-XX, (http://www3.epa.gov/pesticides/chem_search/reg_actions/reregistration/fs_PC-014504_1-Sep-05.pdf).
  7. Faggio, C., Pagano, M., Alampi, R., Vazzana, I. & Felice, MR. (2016). Cytotoxicity, haemolymphatic parameters, and oxidative stress following exposure to sublethal concentrations of quaternium-15 in Mytilus galloprovincialis. Aquatic Toxicology, 180: 258-265. DOI: 10.1016/j.aquatox.2016.10.010
  8. Karaca, M., Varışlı, L., Korkmaz, K., Özaydın, O., perçin, F., Orhan, H. (2014). Organochlorine pesticides and antioxidant enzymes are inversely correlated with liver enzyme gene expression in Cyrinus carpio. Toxicology Letters, 230:198-207. DOI: 10.1016/j.toxlet.2014.02.013

Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Araştırma Makalesi

Yayımlanma Tarihi

15 Aralık 2019

Gönderilme Tarihi

5 Şubat 2019

Kabul Tarihi

2 Temmuz 2019

Yayımlandığı Sayı

Yıl 2019 Cilt: 36 Sayı: 4

Kaynak Göster

APA
Kayhan, F. E., Kaymak, G., Esmer Duruel, H. E., & Kızılkaya, Ş. (2019). Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb. Ege Journal of Fisheries and Aquatic Sciences, 36(4), 325-328. https://doi.org/10.12714/egejfas.36.4.02