Effect of natural adsorbent Clinoptilolite on some hematological parameters of Cyprinus carpio

Okay Oğuztürk; Nuray Çiftçi; Bedii Cicik

doi:10.12714/egejfas.2018.35.4.11

EN TR

Effect of natural adsorbent Clinoptilolite on some hematological parameters of Cyprinus carpio

Abstract

Effect of natural chelating agent clinoptilolite on hematocrit level, erythrocyte numbers, mean corpuscular volume (MCV), erythrocyte and erythrocyte nucleus area of Cyprinus carpio were determined after exposing the animals to 2, 4, 8, 16, 32 gL-1 clinoptilolite (0.40 µ) (3, 7, 21 and 30 days). Hematocrit levels were determined using microhematocrit methods and erythrocyte numbers, erythrocyte and its nucleus areas and MCV were determined using microscopic methods.

Erythrocyte number and hematocrit levels decreased at 16 and 32 g L-1 of clinoptilolite with compared to control group in except 3 and 7 days of exposure (p<0.05). Clinoptilolite increased MCV, erythrocyte and its nucleus areas compared with the control. Erythrocyte numbers, erythrocyte and its nucleus area increased at 2, 4 and 8 g L-1 concentrations of clinoptilolite during 7, 21 and 30 days exposure compared to 3 days exposure while hematocrit and MCV decreased. Erythrocyte numbers, erythrocyte and its nucleus area decreased at 32 g L-1 concentrations of clinoptilolite during 7, 21 and 30 days exposure compared to 3 days exposure while hematocrit and MCV increased (p<0.05).

Changes observed in hematocrit level and erythrocyte morphology might be due to the effect of studied concentrations of clinoptilolite on both gill and blood cell permeabilities.

Keywords

Cyprinus carpio,clinoptilolite,adsorbent,blood

Doğal bir adsorban olan Klinoptilolitin, Cyprinus carpio ’nun bazı hematolojik parametreleri üzerine etkileri

Öz

Araştırmada, doğal bir şelatlayıcı ajan olan klinoptilolitin (0,40 µ) 2, 4, 8, 16, 32 g/l’lik ortam derişimlerinin 3, 7, 21 ve 30 gün sürelerde, Cyprinus carpio’nun hematokrit düzeyi, eritrosit sayısı, ortalama eritrosit hacmi (MCV), eritrosit ve eritrosit nukleus alanı üzerine etkilerinin belirlenmesi amaçlanmıştır. İncelenen parametrelerden hematokrit düzeyinin analizinde mikrohematokrit yöntemi, eritrosit sayısı, MCV, eritrosit ve eritrosit nukleus alanının analizinde mikroskobik yöntemler kullanılmıştır.

Eritrosit sayısı ve hematokrit düzeyinin klinoptilolitin 16 ve 32 g/l ortam derişimlerinin 3 ve 7 günlük etki süreleri dışında, belirlenen süre ve derişimlerinin etkisinde kontrole göre düştüğü belirlenmiştir (p<0,05). MCV, eritrosit ve eritrosit nukleus alanının klinoptilolitin etkisinde kontrole oranla artış gösterdiği saptanmıştır. Klinoptilolitin 2, 4 ve 8 g/l derişimleri etkisinde eritrosit sayısı, eritrosit ve eritrosit nükleus alanı 3. güne oranla 7, 21 ve 30 gün etkisinde artarken, hematokrit düzeyi ile MCV düşmüştür. Klinoptilolitin 32 g/l derişimi etkisinde ise eritrosit sayısı, eritrosit ve eritrosit nükleus alanı 3. güne oranla 7, 21 ve 30 gün sürelerde düşmüş, hematokrit düzeyi ile MCV artmıştır (p<0,05).

Hematokrit düzeyi ile eritrosit morfolojisinde meydana gelen değişimlerin klinoptilolitin uygulanan derişim ve etki süresine bağlı olarak gerek solungaç gerekse kan hücrelerinin membran permeabilitesindeki bozukluktan kaynaklanabileceği olasıdır.

Anahtar Kelimeler

Cyprinus carpio,klinoptilolit,adsorban,kan

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Okay Oğuztürk
0000-0001-6457-1775
Türkiye

Nuray Çiftçi ^*
0000-0002-2925-0332
Türkiye

Bedii Cicik
0000-0003-3982-9943
Türkiye

Yayımlanma Tarihi

15 Aralık 2018

Gönderilme Tarihi

16 Mayıs 2018

Kabul Tarihi

4 Ekim 2018

Yayımlandığı Sayı

Yıl 2018 Cilt: 35 Sayı: 4

DOI

https://doi.org/10.12714/egejfas.2018.35.4.11

IZ

https://izlik.org/JA68CB76HT

Kaynak Göster

RIS / Bibtex

APA

Oğuztürk, O., Çiftçi, N., & Cicik, B. (2018). Effect of natural adsorbent Clinoptilolite on some hematological parameters of Cyprinus carpio. Ege Journal of Fisheries and Aquatic Sciences, 35(4), 455-462. https://doi.org/10.12714/egejfas.2018.35.4.11