Gökkuşağı alabalığı (Oncorhynchus mykiss)’nın inaktive Flavobacterium psychrophilum içeren aljinat mikropartikülleri ile immunizasyonu

Ünal İspir; Cebrahil Türk; Muammer Kırıcı; M. Reşit Taysı; Mikail Özcan

doi:10.12714/egejfas.2016.33.3.07

EN TR

Immunization of rainbow trout (Oncorhynchus mykiss) with alginate microparticles containing inactivated Flavobacterium psychrophilum

Abstract

In this study, survival rate of rainbow trout (Oncorhynchus mykiss), fed for 28 days with sodium alginate microparticles containing Flavobacterium psychrophilum inactivated with formalin, against diseases is determined. The fishes are divided into six groups in terms of feeding method; control group, sodium alginated group, fishmeals including 2.5 and 10 mg/g F. psychrophilum, fishmeals of 2.5 and 10 mg/g F. psychrophilum encapsulated with alginate microparticles. Following by oral immunization, experimental infection is created by bathing them in 3.2x107 CFU/ml F. psychrophilum for 1 hour at 53. and 87. days and relative percent survival (RPS) rates are determined. At fifth third day of immunization, while mortality rate of 79.2% is observed for control group, the highest RPS value of 62.1 is found for the group fed with 10 mg/g F. psychrophilum alginate microparticles. At eighty seventh day in infectious F. psychrophilum, while control group’s mortality rate is found 74.8, the rest of groups RPS rates are determined 30.48, 35.3, 40.10, 50.25 and 56.68 respectively. Based on these findings, it can be asserted that protectiveness of fishes, fed with fishmeal encapsulated with sodium alginate microparticles by using inactivated bacterial cells, is not achieved at a sufficient level.

Keywords

Gökkuşağı alabalığı (Oncorhynchus mykiss)’nın inaktive Flavobacterium psychrophilum içeren aljinat mikropartikülleri ile immunizasyonu

Öz

Bu çalışmada, formalin ile inaktive edilmiş Flavobacterium psychrophilum'un sodyum aljinat ile kaplanmış mikropartiküller ile 28 gün için beslenen gökkuşağı alabalık (Oncorhynchus mykiss)'larının hastalığa karşı koruma oranları tespit edildi. Balıklar kontrol, sodyum aljinatlı grup, 2.5 ve 10 mg/g F. psychrophilum içeren yem, 2.5 ve 10 mg/g F. psychrophilum’un aljinat mikropartikülleri ile kapsüllendiği yem ile beslenen olmak üzere altı gruba ayrıldı. Oral immunizasyonu takiben 53 ve 87. gün 3.2x107 CFU/ml F. psychrophilum ile 1 saat banyo yaptırılarak deneysel enfeksiyon oluşturuldu ve nispi hayatta kalma (Relative percent survival, RPS) oranları belirlendi. İmmunizasyonun 53. gününde kontrol grubu balıklarında %79.2 oranında mortalite gözlenirken en yüksek RPS değeri 62.1 ile 10 mg/g F. psychrophilum’un aljinat mikropartikülleri ile beslenen grupta tespit edildi. F. psychrophilum ile 87. günde yapılan enfeksiyonda kontrol grubunda 74.8 mortalite oranı bulunmuşken diğer gruplardaki RPS oranı sırasıyla 30.48, 35.30, 40.10, 50.25 ve 56.68 olarak belirlendi. Bu bulgulara göre inaktive bakteri hücreleri kullanılarak sodyum aljinat mikropartikülleri ile kapsüllenen yem ile beslenen balıklarda koruyuculuğun yeterli düzeyde oluşmadığı ileri sürülebilir

Anahtar Kelimeler

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Ünal İspir

Cebrahil Türk

Muammer Kırıcı

M. Reşit Taysı

Mikail Özcan

Yayımlanma Tarihi

15 Ağustos 2016

Gönderilme Tarihi

12 Ağustos 2016

Kabul Tarihi

-

Yayımlandığı Sayı

Yıl 2016 Cilt: 33 Sayı: 3

DOI

https://doi.org/10.12714/egejfas.2016.33.3.07

IZ

https://izlik.org/JA36SE82TM

Kaynak Göster

RIS / Bibtex

APA

İspir, Ü., Türk, C., Kırıcı, M., Taysı, M. R., & Özcan, M. (2016). Gökkuşağı alabalığı (Oncorhynchus mykiss)’nın inaktive Flavobacterium psychrophilum içeren aljinat mikropartikülleri ile immunizasyonu. Ege Journal of Fisheries and Aquatic Sciences, 33(3), 233-240. https://doi.org/10.12714/egejfas.2016.33.3.07

Cited By

Formalin ile İnaktive Flavobacterium psychrophilum’un Gökkuşağı Alabalığı (Oncorhynchus mykkiss)’nın Antioksidan Enzimlerine Etkisi

Türk Tarım ve Doğa Bilimleri Dergisi

https://doi.org/10.30910/turkjans.843023