Determination of anti-cancer and antioxidant properties of protein extracts obtained from aquatic Helophorus (Coleoptera: Helophoridae) insects

Tuba Elhazar; Bülent Kaya; Fatma Caf

doi:10.12714/egejfas.40.1.05

TR EN

Sucul Helophorus (Coleoptera: Helophoridae) böceklerinden elde edilen protein ekstraktlarının anti-kanser ve antioksidan özelliklerinin belirlenmesi

Öz

Bu çalışmada, Helophorus (Coleoptera: Helophoridae) cinsine ait sucul böcek Helophorus aquaticus ve Helophorus syriacus türlerinden protein ekstraksiyonu yapılmıştır. Böcek türleri 2017 Mayıs-Haziran ayları arasında ve Bingöl il merkezi ve ilçelerinden çeşitli akarsu, kaynak, dere, birikinti ve sıcak su gözelerinin sığ kesimlerinden toplandı. Protein miktarı H. aquaticus’da 34,78 mg/mL, H. syriacus’da 35,14 mg/mL olarak belirlenmiştir. Daha sonra elde edilen protein ekstraktlarının antioksidan kapasitesi incelendi. Metal şelatlama aktivitesi H. aquaticus ve H. syriacus için sırasıyla %90-88,5, DPPH uzaklaştırma aktivitesi %53,19-61,7 olarak belirlendi. Her iki türe ait protein numuneleri, in vitro hücre kültürü ile PC-3 (prostat kanseri) hücrelerinde WST-1 ile hücre canlılığı açısından test edildi. Test sonuçlarının incelenmesi üzerine, çalışılan türlerin her ikisinden alınan protein ekstraktları, hücre inhibisyonunda bir azalmaya neden oldu. En yüksek hücre inhibisyonu, 1000 μg/mL böcek proteini ekstresi eklenen numunelerde gözlendi. Bu çalışmada, etkin doz belirlendikten sonra Western blot tekniği ile apoptozu sağlayan protein ekspresyonu incelenmiştir. Western blot tekniği ile Cyt-C ve Caspase-3 proteinlerine bakılarak, apoptoz dışı hücre ölümünde in vitro PC-3 hattı için her iki türden protein ekstraktlarının etkinliği etkili bir şekilde gösterildi. Çalışma sonucunda böcek proteinlerinin western blot tekniği ile hücre ölümünü sağlayan proteinlerin üretimini desteklediği gösterildi.

Anahtar Kelimeler

Determination of anti-cancer and antioxidant properties of protein extracts obtained from aquatic Helophorus (Coleoptera: Helophoridae) insects

Abstract

In this study, protein extraction was performed from the aquatic insect Helophorus aquaticus (Linnaeus, 1758) and Helophorus syriacus (Kuwert, 1885) species belonging to the genus Helophorus (Coleoptera: Helophoridae). Then, these protein extracts were studied in vitro. These species were collected from the shallow parts of various streams, springs, creeks, ponds and hot springs from Bingöl city centre and its districts between May to June 2017. The protein amount was determined 34.78 mg/mL in the H. aquaticus and 35.14 mg/mL in the H. syriacus, after that he antioxidant capacity of protein extracts was examined. Metal chelating activity was determined as 90-88.5% and DPPH removal activity 53.19-61.7% for H. aquaticus and H. syriacus respectively. Protein samples belonging to both species were tested for cell vitality with WST-1 in PC-3 (prostate cancer) cells with in vitro cell culture. Upon examination of the test results, it has been found out that protein extracts from both of the studied species caused a decrease in cell inhibition. The highest cell inhibition was observed in samples with 1000 μg/mL insect protein extract added. In this study, protein expression providing apoptosis was examined with the Western blot technique after the effective dose was established. By looking at the proteins of Cyt-C and Caspase 3 with the Western blot technique, the efficacy of the protein extracts from both species was demonstrated effectively for the in vitro PC-3 line in non-apoptosis cell death. As a result of the study, insect proteins were shown to support the production of proteins that ensure cell death with the western blot technique.

Keywords

Destekleyen Kurum

This works were supported by the Science Research Foundation of Bingol University of Turkey

Proje Numarası

Project number: BAP-FEF.2017.00.003

Teşekkür

We thank Abdullah Mart for the identification of the species. We would also like to thank Ekrem Darendelioğlu for his help and laboratory for the Western Blot part of the study.

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Tuba Elhazar
0000-0003-3632-1159
Türkiye

Bülent Kaya
0000-0002-1216-6441
Türkiye

Fatma Caf ^*
0000-0002-0363-4848
Türkiye

Yayımlanma Tarihi

15 Mart 2023

Gönderilme Tarihi

2 Eylül 2022

Kabul Tarihi

13 Ocak 2023

Yayımlandığı Sayı

Yıl 2023 Cilt: 40 Sayı: 1

DOI

https://doi.org/10.12714/egejfas.40.1.05

IZ

https://izlik.org/JA75UL93AR

Kaynak Göster

RIS / Bibtex

APA

Elhazar, T., Kaya, B., & Caf, F. (2023). Determination of anti-cancer and antioxidant properties of protein extracts obtained from aquatic Helophorus (Coleoptera: Helophoridae) insects. Ege Journal of Fisheries and Aquatic Sciences, 40(1), 35-42. https://doi.org/10.12714/egejfas.40.1.05

Cited By

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Frontiers in Pharmacology

https://doi.org/10.3389/fphar.2024.1345281