Yıl 2019, Cilt 36 , Sayı 3, Sayfalar 245 - 253 2019-09-15

Cıva toksisitesi üzerine selenyumun koruyucu rolünün Oreochromis niloticus (Linnaeus, 1758)’ta katalaz, süperoksit dismutaz ve malondialdehit parametreleri ile değerlendirilmesi
Evaluation of protective role of selenium on mercury toxicity by superoxide dismutase, catalase and malondialdehyde parameters in Oreochromis niloticus (Linnaeus, 1758)

Özgür FIRAT [1] , Özlem KAYA [2]




Bu araştırmada cıva (Hg) toksisitesini ve bu toksisite üzerine selenyumun (Se) koruyucu rolünü belirlemek için Oreochromis niloticus’un dokularındaki oksidatif stres parametreleri çalışılmıştır. Bu amaçla balıklar 4 ve 21 günlük sürelerle 0.01 ve 0.05 ppm Hg ile 0.01+0.01 ve 0.05+0.05 ppm Hg+Se karışımlarının etkisine bırakılmış ve solungaç ve karaciğer süperoksit dismutaz (SOD) ve katalaz (CAT) aktiviteleri ile malondialdehit (MDA) düzeyleri belirlenmiştir. Hg’nin tek başına ve Se ile birlikte etkisinde incelenen oksidatif stres parametrelerinde dokuya, ortam derişimine ve etki süresine bağlı olarak önemli değişimlerin meydana geldiği belirlenmiştir. Cıvanın tek başına ve cıva+selenyum karışımının etkisinde özellikle de yüksek ortam derişimlerinde solungaç ve karaciğer SOD ve CAT aktiviteleri, 4 günlük süre sonunda anlamlı bir artış gösterirken (P˂0.05); 21 günlük süre sonunda ise anlamlı bir azalma göstermiştir (P˂0.05). Hg ve Hg+Se karışımının etkisinde her iki dokuda 4 günlük süre sonunda tüm ortam derişimlerinde önemli bir değişim göstermeyen (P˃0.05) MDA düzeyinin, 21 günlük süre sonunda yüksek ortam derişimlerinde anlamlı bir artış gösterdiği saptanmıştır (P˂0.05). Sonuç olarak O. niloticus’ta belirlenen SOD ve CAT aktiviteleri ile MDA düzeylerindeki artış veya azalışların cıva+selenyum karışımına oranla cıvanın tek başına etkisinde daha fazla olduğu ve selenyumun cıvanın oksidatif toksisitesi üzerine koruyucu bir etkiye sahip olduğu belirlenmiştir.






In this research, to determine toxicity of mercury (Hg) and whether selenium (Se) has any role in protection of this toxicity, it was investigated the alterations in oxidative stress parameters in tissues of Oreochromis niloticus. For this purpose, fish were exposed to 0.01 and 0.05 ppm Hg and 0.01 ppm Hg+0.01 ppm Se and 0.05 ppm Hg+0.05 ppm Se for 4 and 21 days and activities of superoxide dismutase (SOD) and catalase (CAT) and levels of malondialdehyde (MDA) in gill and liver were determined. It was observed significant changes in all analyzed parameters due to tissue, medium concentration and exposure period in the exposure of mercury alone and Hg+Se mixtures. In Hg alone, and in combination with Se especially in their higher medium concentrations, activities of SOD and CAT in the gill and liver significantly increased at 4 days (P˂0.05), while they significantly decreased at 21 days (P˂0.05). In the exposure of Hg and Hg+Se and in the both tissues, it not determined significant alteration in the MDA levels at 4 days (P˃0.05), while they elevated in their higher concentrations at 21 days (P˂0.05). In conclusion, it was determined the increases or decreases in activities of SOD and CAT and levels of MDA in O. niloticus were higher in the Hg alone than Hg+Se mixtures and selenium has a protective effect on oxidative toxicity of mercury.




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Birincil Dil tr
Konular Fen
Bölüm Makaleler
Yazarlar

Orcid: 0000-0002-9683-8945
Yazar: Özgür FIRAT (Sorumlu Yazar)
Kurum: ADIYAMAN ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0002-2885-1865
Yazar: Özlem KAYA
Kurum: ADIYAMAN ÜNİVERSİTESİ
Ülke: Turkey


Destekleyen Kurum Adıyaman Üniversitesi
Proje Numarası FEFYL2014-0010
Teşekkür Bu çalışma yüksek lisans tez çalışması olup FEFYL2014-0010 nolu Adıyaman Üniversitesi (ADYÜ) Bilimsel Araştırma Projesi ile yürütülmüş olup ADYÜ Bilimsel Araştırma Projeleri Koordinasyon Biriminin değerli yöneticilerine ve çalışanlarına teşekkür ederiz.
Tarihler

Başvuru Tarihi : 25 Ocak 2019
Kabul Tarihi : 17 Haziran 2019
Yayımlanma Tarihi : 15 Eylül 2019

APA FIRAT, Ö , KAYA, Ö . (2019). Cıva toksisitesi üzerine selenyumun koruyucu rolünün Oreochromis niloticus (Linnaeus, 1758)’ta katalaz, süperoksit dismutaz ve malondialdehit parametreleri ile değerlendirilmesi. Ege Journal of Fisheries and Aquatic Sciences , 36 (3) , 245-253 . DOI: 10.12714/egejfas.2019.36.3.05