Abstract
Türkiye’deki Pseudophoxinus türlerin yarısının karyolojik özellikleri belirtilmiştir. Beyşehir gölünde yaygın olan P. anatolicus’un karyolojik özelliklerini belirlemek için planlanan bu çalışmada örnekler Çiftlik köyü kıyısından yakalandı. Yakalanan bireylerin karyolojik analizleri yapıldı ve elde edilen slaytlara sırayla giemsa boyama, C-bantlama ve Ag-NOR boyama uygulandı. Bu türün kromozom seti 2 çift metasentrik, sekiz çift submetasentrik, iki çift subtelosentrik ve üç çift akrosentrik kromozomdan oluşmaktadır. Koyu ve açık C-bantlar bazı kromozomların sentromerik bölgelerinde gözlemlendi. Aktif Ag-NOR’lar iki çift kromozomun kısa kolunun telomerik bölgesinde tespit edildi. Sonuçlarımızın diğer Pseudophoxinus türlerinkine bazı farklılıklar hariç benzer tarafları vardır ve Anadolu yağ balığının da diğer Pseudophoxinus türlerindeki gibi korunmuş karyotipe sahip olduğu belirlendi.
Keywords
Project Number
21211009
References
- Arslan, A., & Gündoğdu H. (2016). Cytogenetic studies on the endemic Beyşehir nase, Chondrostoma beysehirense (Bogutskaya, 1997) in Türkiye. Caryologia, 69(2), 116 120. https://doi.org/10.1080/00087114.2015.1109958
- Bertollo, L., Cioffi, M., & Moreira-Filho, O. (2015). Direct chromosome preparation from freshwater teleost fishes. In C. Ozouf-Costaz, E. Pisano, F. Foresti, & L. Almeida Toledo (Eds.), Fish Cytogenetic Techniques (Chondrichthyans and Teleosts) (pp. 21-26). United States: CRC Press.
- Bueno, V., Venere, P.C., Thums Konerat, J., Zawadzki, C.H., Vicari, M.R., & Margarido, V.P. (2014). Physical mapping of the 5S and 18S rDNA in ten species of Hypostomus Lacépède 1803 (Siluriformes: Loricariidae): evolutionary tendencies in the genus. Scientific World Journal, 943825, https://doi.org/10.1155/2014/943825
- Çiçek, E., Sungur, S., & Fricke, R. (2020). Freshwater lampreys and fishes of Türkiye; a revised and updated annotated checklist 2020. Zootaxa, 4809(2), 241-270. https://doi.org/10.11646/zootaxa.4809.2.2
- Diniz, D., Laudicina, A., & Bertollo, L.A. (2009). Chromosomal location of 18S and 5S rDNA sites in Triportheus fish species (Characiformes, Characidae). Genetics and Molecular Biology, 32, 37-41. https://doi.org/10.1590/S1415-47572009005000017
- Ergene, S., Karahan, A., & Kuru, M. (2010). Cytogenetic analysis of Pseudophoxinus antalyae, Bogustkaya, 1992 (Pisces: Cyprinidae) from the Eastern Mediterranean River Basin. Türkiye. Turkish Journal of Zoology, 34(1), 111-117. https://doi.org/10.3906/zoo-0807-33
- Gaffaroğlu, M., Karasu Ayata, M., & Ünal Karakuş, S. (2022). Cytogenetic characteristics of two species of the genus Pseudophoxinus Bleeker, 1860 (Cypriniformes: Leuciscidae) from Anatolia, Turkey. Acta Zoologica Bulgarica, 74(3), 335-342.
- Howell, W.M., & Black, D.A. (1980). Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia, 36, 1014-1015. https://doi.org/10.1007/BF01953855
- Karasu, M., Yüksel, E., & Gaffaroğlu, M. (2011). Karyotype, NORs, and C-banding analysis of Pseudophoxinus firati Bogutskaya, Küçük, & Atalay, 2007 (Actinopterygii, Cyprinidae) in the Euphrates River. Türkiye. Turkish Journal of Zoology, 35(6), 865-868. https://doi.org/10.3906/zoo-0912-129
- Karasu Ayata, M., Yüksel, E., & Gaffaroğlu, M. (2016). Cytogenetic studies on six species of the leuciscine genus Pseudophoxinus Bleeker, 1860 (Teleostei, Cyprinidae). Caryologia, 69(3), 215 222. https://doi.org/10.1080/00087114.2016.1169089
- Küçük, F., Atalay, M.A., Güçlü, S.S., & Gülle, İ. (2012). The Morphological Characteristics of Pseudophoxinus (Teleostei:Cyprinidae) Species of Turkey and their Zoogeographic Distribution. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 8(2), 1-9. (in Turkish with English abstract)
- Küçük, F., Gülle, I.,& Güçlü, S.S. (2016). Pseudophoxinus iconii, a new species of spring minnow from Central Anatolia (Teleostei: Cyprinidae). Ichthyological Exploration of Freshwaters, 27(3), 283-288.
- Levan, A., Fredga, K., & Sandberg, A.A. (1964). Nomenclature for centromeric position on chromosomes. Hereditas, 52, 201 220. https://doi.org/10.1111/j.1601-5223.1964.tb01953.x
- Perea, S., Bohme, M., Zupancic, P., Freyhof, J., Sanda, R., Özulug, M., Abdoli, A., & Doadrio, I. (2010). Phylogenetic relationships and biogeographical patterns in Circum-Mediterranean subfamily Leuciscinae (Teleostei, Cyprinidae) inferred from both mitochondrial and nuclear data. BMC Evolutionary Biology 10, 265. https://doi.org/10.1186/1471-2148-10-265
- Sumner, A. (1972). A simple technique for demonstrating centromeric heterochromatin. Experimental Cell Research, 75, 304 306. https://doi.org/10.1016/0014-4827(72)90558-7
- Ünal, S., & Gaffaroğlu, M. (2016). Karyology of six cyprinid fishes from Seyhan and Ceyhan rivers in Anatolia. Caryologia, 69(4), 362-369. https://doi.org/10.1080/00087114.2016.1247328
- Ünal, S, Gaffaroğlu, M., Karasu Ayata, M., & Yüksel, E. (2014). Karyotype, C-banding and AgNORs of two endemic leuciscine fish, Pseudophoxinus crassus (Ladiges, 1960) and P. hittitorum Freyhof & Özulug, 2010 (Teleostei, Cyprinidae). Comparative Cytogenetics, 8(4), 249-257. https://doi.org/10.3897/CompCytogen.v8i4.7623
Abstract
The karyological characteristics of nearly half of the Pseudophoxinus species in Türkiye were determined. In this study, it is planned to determine the karyological characteristics of P. anatolicus, which is common in Beyşehir Lake, specimens were caught from the coast at Çiftlik village. The captured specimen were karyological analysed and Giemsa staining, C-banding and Ag-NOR staining were applied to the slides that obtained. The chromosome set of this species consists of 12 pairs of metacentric, eight pairs of submetacentric, two pairs of subtelocentric and three pairs of acrocentric chromosomes. Dark and slightly C-bands were observed in the centromeric regions of some chromosomes. Active Ag-NORs were detected in the telomeric region of the short arm of two pairs of chromosomes. Our results are similar to those of other Pseudophoxinus species except for some differences and it was determined that Anatolian minnow has a conserved karyotype like other Pseudophoxinus species.
Keywords
Ethical Statement
The samples in this study were collected with the permission of the TR Ministry of Forestry and Water Affairs (Permit No: E-21264211-288.04-3435924). This permission replaces the local ethics committee permission per 8/L of the regulation "On Working Procedures and Principles of Animal Experiments Ethics Committees" prepared by the Ministry of Forestry and Water Affairs and published in the Official Gazette on February 15, 2014.
Supporting Institution
This study was financed with a grant from the Scientific Research Projects Coordination Board of Selçuk University
Project Number
21211009
Thanks
The Scientific Research Projects Coordination Board of Selçuk University (BAP)
References
- Arslan, A., & Gündoğdu H. (2016). Cytogenetic studies on the endemic Beyşehir nase, Chondrostoma beysehirense (Bogutskaya, 1997) in Türkiye. Caryologia, 69(2), 116 120. https://doi.org/10.1080/00087114.2015.1109958
- Bertollo, L., Cioffi, M., & Moreira-Filho, O. (2015). Direct chromosome preparation from freshwater teleost fishes. In C. Ozouf-Costaz, E. Pisano, F. Foresti, & L. Almeida Toledo (Eds.), Fish Cytogenetic Techniques (Chondrichthyans and Teleosts) (pp. 21-26). United States: CRC Press.
- Bueno, V., Venere, P.C., Thums Konerat, J., Zawadzki, C.H., Vicari, M.R., & Margarido, V.P. (2014). Physical mapping of the 5S and 18S rDNA in ten species of Hypostomus Lacépède 1803 (Siluriformes: Loricariidae): evolutionary tendencies in the genus. Scientific World Journal, 943825, https://doi.org/10.1155/2014/943825
- Çiçek, E., Sungur, S., & Fricke, R. (2020). Freshwater lampreys and fishes of Türkiye; a revised and updated annotated checklist 2020. Zootaxa, 4809(2), 241-270. https://doi.org/10.11646/zootaxa.4809.2.2
- Diniz, D., Laudicina, A., & Bertollo, L.A. (2009). Chromosomal location of 18S and 5S rDNA sites in Triportheus fish species (Characiformes, Characidae). Genetics and Molecular Biology, 32, 37-41. https://doi.org/10.1590/S1415-47572009005000017
- Ergene, S., Karahan, A., & Kuru, M. (2010). Cytogenetic analysis of Pseudophoxinus antalyae, Bogustkaya, 1992 (Pisces: Cyprinidae) from the Eastern Mediterranean River Basin. Türkiye. Turkish Journal of Zoology, 34(1), 111-117. https://doi.org/10.3906/zoo-0807-33
- Gaffaroğlu, M., Karasu Ayata, M., & Ünal Karakuş, S. (2022). Cytogenetic characteristics of two species of the genus Pseudophoxinus Bleeker, 1860 (Cypriniformes: Leuciscidae) from Anatolia, Turkey. Acta Zoologica Bulgarica, 74(3), 335-342.
- Howell, W.M., & Black, D.A. (1980). Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia, 36, 1014-1015. https://doi.org/10.1007/BF01953855
- Karasu, M., Yüksel, E., & Gaffaroğlu, M. (2011). Karyotype, NORs, and C-banding analysis of Pseudophoxinus firati Bogutskaya, Küçük, & Atalay, 2007 (Actinopterygii, Cyprinidae) in the Euphrates River. Türkiye. Turkish Journal of Zoology, 35(6), 865-868. https://doi.org/10.3906/zoo-0912-129
- Karasu Ayata, M., Yüksel, E., & Gaffaroğlu, M. (2016). Cytogenetic studies on six species of the leuciscine genus Pseudophoxinus Bleeker, 1860 (Teleostei, Cyprinidae). Caryologia, 69(3), 215 222. https://doi.org/10.1080/00087114.2016.1169089
- Küçük, F., Atalay, M.A., Güçlü, S.S., & Gülle, İ. (2012). The Morphological Characteristics of Pseudophoxinus (Teleostei:Cyprinidae) Species of Turkey and their Zoogeographic Distribution. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 8(2), 1-9. (in Turkish with English abstract)
- Küçük, F., Gülle, I.,& Güçlü, S.S. (2016). Pseudophoxinus iconii, a new species of spring minnow from Central Anatolia (Teleostei: Cyprinidae). Ichthyological Exploration of Freshwaters, 27(3), 283-288.
- Levan, A., Fredga, K., & Sandberg, A.A. (1964). Nomenclature for centromeric position on chromosomes. Hereditas, 52, 201 220. https://doi.org/10.1111/j.1601-5223.1964.tb01953.x
- Perea, S., Bohme, M., Zupancic, P., Freyhof, J., Sanda, R., Özulug, M., Abdoli, A., & Doadrio, I. (2010). Phylogenetic relationships and biogeographical patterns in Circum-Mediterranean subfamily Leuciscinae (Teleostei, Cyprinidae) inferred from both mitochondrial and nuclear data. BMC Evolutionary Biology 10, 265. https://doi.org/10.1186/1471-2148-10-265
- Sumner, A. (1972). A simple technique for demonstrating centromeric heterochromatin. Experimental Cell Research, 75, 304 306. https://doi.org/10.1016/0014-4827(72)90558-7
- Ünal, S., & Gaffaroğlu, M. (2016). Karyology of six cyprinid fishes from Seyhan and Ceyhan rivers in Anatolia. Caryologia, 69(4), 362-369. https://doi.org/10.1080/00087114.2016.1247328
- Ünal, S, Gaffaroğlu, M., Karasu Ayata, M., & Yüksel, E. (2014). Karyotype, C-banding and AgNORs of two endemic leuciscine fish, Pseudophoxinus crassus (Ladiges, 1960) and P. hittitorum Freyhof & Özulug, 2010 (Teleostei, Cyprinidae). Comparative Cytogenetics, 8(4), 249-257. https://doi.org/10.3897/CompCytogen.v8i4.7623
Details
| Primary Language | English |
|---|---|
| Subjects | Ecology (Other) |
| Journal Section | Articles |
| Authors | |
| Project Number | 21211009 |
| Early Pub Date | March 11, 2024 |
| Publication Date | March 15, 2024 |
| Submission Date | October 30, 2023 |
| Acceptance Date | January 16, 2024 |
| Published in Issue | Year 2024Volume: 41 Issue: 1 |