Seasonal changes in antioxidant enzyme activities of Garra rufa (Heckel, 1843) in Göynük Stream (Bingöl, Türkiye)

Muammer Kırıcı; Nurgül Sen Özdemir; Fatma Caf; Mustafa Koyun

doi:10.12714/egejfas.40.3.03

TR EN

Göynük Çayı'nda (Bingöl, Türkiye) Garra rufa'nın (Heckel, 1843) antioksidan enzim aktivitelerindeki mevsimsel değişimleri

Öz

Bu çalışmada, Murat Nehri’nin en önemli kollarından biri olan Göynük Çayı’ndan yakalanan Garra rufa (Heckel, 1843) balıklarının solungaç, böbrek, kas ve karaciğer dokularında mevsimlere bağlı olarak antioksidan enzim aktivitelerindeki seviyelerindeki değişimler incelendi. Bu amaç doğrultusunda balıklar, her ay düzenli olarak belirlenen iki istasyondan (Garip ve Ilıcalar) yakalanarak laboratuvara getirildi. Dokularda enzim aktiviteleri ve MDA seviyesi spektrofotometrik yöntemlerle belirlendi. Çalışma sonucunda, genel olarak iki istasyon arasında tüm dokularda parametreler arasındaki fark istatistiki olarak önemli bulunmuştur (p < 0.05). Ancak karaciğer dokusunda GR enziminde tüm mevsimlerde istasyonlar arasındaki fark istatistiki olarak önemli olmadığı belirlendi. Bununla beraber, her iki istasyonda da genel olarak mevsimler arasındaki farklılıkların önemli olduğu gözlemlendi. Çalışmada, GR ve G6PD enzim aktiviteleri diğer enzim aktiviteleri ile kıyaslandığında düşükken, CAT ve SOD aktiviteleri ise diğerlerinden yüksektir. Dokular arasında ise kas dokusundaki enzim aktiviteleri, diğer dokulardaki aktivitelerden daha düşüktür.

Anahtar Kelimeler

Supporting Institution

Bingöl Üniversitesi

Project Number

BAP-ZF.2016.00.006

Thanks

This study was supported by the Research Fund (BAP-ZF.2016.00.006) of Bingöl University (Turkey).

References

Aebi, H. (1983). Catalase. In H.U. Bergmeyer (Ed.), Methods in Enzymatic Analysis (pp. 673-684). New York: Academic Press. https://doi.org/10.1016/B978-0-12-091302-2.50032-3
Aras, N.M., Bayır, A., Sirkecioğlu, A.N., Bayır, M., Aksakal E., & Haliloğlu, H.İ. (2009). Seasonal changes in antioxidant defence system of liver and gills of Salmo trutta caspius, Salmo trutta labrax and Salmo trutta macrostigma. Journal of Fish Biology, 74, 842 856. https://doi.org/10.1111/j.1095-8649.2008.02164.x
Barda, I., Purina. I., Rimsa. E., & Balode. M. (2014). Seasonal dynamics of biomarkers in infaunal clam Macoma balthica from the Gulf of Riga (Baltic Sea). Journal of Marine Systems, 129, 150 156. https://doi.org/10.1016/j.jmarsys.2013.05.006
Beutler, E. (1971). Red cell metabolism manual of biochemical methods. Academic Press, London, England.
Beutler, E. (1975). Red cell metabolism manual of biochemical methods. New York, Grune & Strottan Press.
Bocchetti, R., & Regoli, F. (2006). Seasonal variability of oxidative biomarkers, lysosomal parameters, metallothioneins and peroxisomal enzymes in the Mediterranean mussel Mytilus galloprovincialis from Adriatic Sea. Chemosphere, 65(6), 913 921. https://doi.org/10.1016/j.chemosphere.2006.03.049
Borkovic, S.S., Saponjic, J.S., Pavlovic, S.Z., Blagojevic, D.P., & Milosevic, S.M. (2005). The activity of antioxidant defence enzymes in the Mussel Mytilus galloprovincialis from the Adriatic Sea. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 141(4), 366-374. https://doi.org/10.1016/j.cbpc.2005.08.001
Can, E., Cikcikoglu-Yildirim, N., & Erdogan, D. (2017). Seasonal changes in antioxidant defence system on brown trout (Salmo sp.) in Munzur Stream. Fresenius Environmental Bulletin, 26(10), 5936-5941.

Carlberg, C., & Mannervik, B. (1985). Glutathione reductase. In A. Meister (Ed.), Methods in Enzymology (pp 484-495). New York: Academic Press. https://doi.org/10.1016/S0076-6879(85)13062-4
Danabas, D., Yildirim, N.C., Yildirim, N., Onal, A.O., Uslu, G., Unlu, E., Danabas, S., Ergin, C., & Tayhan, N. (2015). Changes in antioxidant defense system in gills of Capoeta umbla caught from Uzuncayir Dam Lake, Turkey. Biochemical Systematics and Ecology, 63, 72-79. https://doi.org/10.1016/j.bse.2015.09.029
Duman, S. (2010). Cyprinion macrostomus Heckel, 1843 and Garra rufa Heckel, 1843 species of fishes that is living in Kangal (Sivas) balikli çermik thermal hot spring with Topardiç stream (Sivas) defines some heamatological parameters and nonspecific immune respond. Doctoral dissertation, Çukurova University, Türkiye (in Turkish with English abstract)
Ergene, S., Cavas, T., Celik, A., Koleli, N., Kaya, F., & Karahan, A. (2007). Monitoring of nuclear abnormalities in peripheralerythrocytes of three fish species from the Goksu Delta (Turkey): genotoxic damage in relation to water pollution. Ecotoxicology, 16, 385 391. https://doi.org/10.1007/s10646-007-0142-4
Filho, D.W., Tribess, T., Gaspari, C., Claudio, F.D., Torres, M.A., & Magalhaes, A.R.M. (2001). Seasonal changes in antioxidant defenses of the digestive gland of the brown mussel (Perna perna). Aquaculture, 203(1-2), 149-158. https://doi.org/10.1016/S0044-8486(01)00599-3
Forget, J., Beliaeff, B., & Bocquené, G. (2003). Acetylcholinesterase activity in copepods (Tigriopus brevicornis) from the Vilaine River estuary, France, as a biomarker of neurotoxic contaminants. Aquatic Toxicology, 62(3), 195–204. https://doi.org/10.1016/S0166-445X(02)00084-X
Gadzala-Kopciuch, R., Berecka, B., Bartoszewic, J., & Buszewski, B. (2004). Some considerations about bioindicators in environmental monitoring. Polish Journal of Environmental Studies, 13(5), 453-462.
Grassberger, M., & Hoch, W. (2006). Ichthyotherapy as alternative treatment for patients with psoriasis: a pilot study. Evidence-Based Complementary and Alternative Medicine, 3(4), 483 488. https://doi.org/10.1093/ecam/nel033
Halliwell, B., & Gutteridge, J.M.C. (2007). Free radicals in biology and medicine (4th ed.). Oxford University Press, New York.
Hotard, S., & Zou, E. (2008). Activity of glutathione S-transferase in the hepatopancreas is not influenced by the molting cycle in the Fidder crab, Uca pugilator. Bulletin of Environmental Contamination and Toxicology, 81, 242–244. https://doi.org/10.1007/s00128-008-9487-5
Ispir, U., Kırıcı, M., Yonar, M.E., & Yonar, S.M. (2017). Response of antioxidant system to formalin in the whole body of rainbow trout, Oncorhynchus mykiss. Cellular and Molecular Biology (Noisy-le-grand), 63(1), 13-16. https://doi.org/10.14715/cmb/2017.63.1.3
Karaaslan, G.M. (2010). The effects of different stress sources on antioxidant enzyme activities in fishes (Cyprinidae) from Sivas Kangal fish spring. Master's thesis, Çukurova University, Türkiye. (in Turkish with English abstract)
Karahan, A. (2007). Comparative investigation of Garra rufa and Garra variabilis by account of sitogenetic and morphometric. Doctoral dissertation, Mersin University, Türkiye. (in Turkish with English abstract)
Khessiba, A., Romeo, M., & Aissa, P. (2005). Effects of some environmental parameters on catalase activity measured in the mussel (Mytilus galloprovincialis) exposed to lindane. Environmental Pollution, 133(2), 275-281. https://doi.org/10.1016/j.envpol.2004.05.035
Kırıcı, M., Kırıcı, M., Işık, M., & Atamanalp, M. (2015). In vitro effects of imidacloprid and lambda-cyhalothrin on Capoeta capoeta umbla kidney glucose 6-phosphate dehydrogenase enzyme. Turkish Journal of Agricultural Research, 2(1), 8-14. https://doi.org/10.19159/tutad.41219
Kırıcı, M., Kırıcı, M., Demir, Y., Beydemir, S., & Atamanalp, M. (2016a). The effect of Al3+ and Hg2+ on glucose 6-phosphate dehydrogenase from Capoeta umbla kidney. Applied Ecology and Environmental Research, 14, 253-264.
Kırıcı, M., Kırıcı, M., Beydemir, Ş., & Bülbül, M. (2016b). In vitro Toxicity effects of some insecticides on gilthead sea bream (Sparus aurata) liver glucose 6-phosphate dehydrogenase. Journal of Applied Biological Sciences, 10(2), 46-50.
Kırıcı, M., Turk, C., Çağlayan, C., & Kırıcı, M. (2017). Toxic effects of copper sulphate pentahydrate on antioxidant enzyme activities and lipid peroxidation of freshwater fish Capoeta umbla (Heckel, 1843) tissues. Applied Ecology and Environmental Research, 15(3), 1685-1696. https://doi.org/10.15666/aeer/1503_16851696
Kırıcı, M., Şen Özdemir, N., Caf, F., & Koyun, M. (2022a). Seasonal changes in antioxidant defense system indicators in the tissues of Cyprinion macrostomus (Heckel, 1843) caught from Göynük Stream (Bingöl, Turkey). Ege Journal of Fisheries and Aquatic Sciences, 39(3), 174-181. https://doi.org/10.12714/egejfas.39.3.01
Kırıcı, M., Taysı, M.R., Kırıcı, M., & Sökmen, T.O. (2022b). Investigation of changes in malondialdehyde level, superoxide dismutase and catalase activity in liver tissue of Capoeta umbla exposed to 2,4-dichlorophenoxyacetic acid. Turkish Journal of Agricultural and Natural Sciences, 9(1), 1–8. https://doi.org/10.30910/turkjans.1011977
Koyun, M., Gül, B., & Korkut, N. (2018). The fish fauna of Göynük Stream (Bingöl). Commagene Journal of Biology, 2(1), 39 47. https://doi.org/10.31594/commagene.403367
Lam, P.K.S., & Gray, J.S. (2003). The use of biomarkers in environmental monitoring programmes. Marine Pollution Bulletin, 46(2), 182-186. https://doi.org/10.1016/S0025-326X(02)00449-6
Lima, I., Moreira, S.M., Osten, J.R., Soares, A.M.V.M., & Guilhermino, L. (2007). Biochemical responses of the marine mussel Mytilus galloprovincialis to petrochemical environmental contamination along the North-Western coast of Portugal. Chemosphere, 66(7), 1230-1242. https://doi.org/10.1016/j.chemosphere.2006.07.057
Linde, A.R., Sánchez-Galán, S., Vallés-Mota, J.P., & García-Vázquez, E. (2001). Metallothionein as bioindicator of freshwater pollution: European eel and brown trout. Ecotoxicology and Environmental Safety, 49(1), 60–73. https://doi.org/10.1006/eesa.2001.2042
Livingstone, D.R. (2001). Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms. Marine Pollution Bulletin, 42(8), 656 666. https://doi.org/10.1016/S0025-326X(01)00060-1
Lowry, O.H., Rosebrough, N.J., Farr, A.L., & Randall, R.J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193(1), 265 275. https://doi.org/10.1016/S0021 9258(19)52451-6
Peebua, P., Kosiyachinda, E.P., Pokethitiyook, E.P., & Kruatrachue, E.M. (2007). Evaluation of alachlor herbicide impacts on Nile tilapia (Oreochromis niloticus) using biochemical biomarkers. Bulletin of Environmental Contamination and Toxicology, 78, 138–141. https://doi.org/10.1007/s00128-007-9027-8
Prevodnik, A., Gardeström, J., Lilja, K., Elfwing, T., McDonagh, B., Petrovic, N., Tedengren, M., Sheehan, D., & Bollner, T. (2007). Oxidative stress in response to xenobiotics in the blue mussel Mytilus edulis L.: evidence for variation along a natural salinity gradient of the Baltic Sea. Aquatic Toxicology, 82(1), 63-71. https://doi.org/10.1016/j.aquatox.2007.01.006
RendÓn-von Osten, J., Ortíz-Arana, A., Guilhermino, L. & Soares, A.M.V.M. (2005). In vivo evaluation of three biomarkers in the mosquitofish (Gambusia yucatana) exposed to pesticides. Chemosphere, 58(5), 627–636. https://doi.org/10.1016/j.chemosphere.2004.08.065
Robillard, S., Beauchamp, G., & Laulier, M. (2003). The role of abiotic factors and pesticide levels on enzymatic activity in the freshwater mussel Anodonta cygnea at three different exposure sites. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 135(1), 49-59. https://doi.org/10.1016/S1532-0456(03)00049-8
Sheehan, D., & Power, A. (1999). Effects of seasonality on xenobiotic and antioxidant defence mechanism of bivalve molluscs. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 123(3), 193-199. https://doi.org/10.1016/S0742-8413(99)00033-X
Slater, T.F. (1984). Overview of methods used for detecting lipid peroxidation. Methods in Enzymology, 105, 283-305. https://doi.org/10.1016/S0076-6879(84)05036-9
Strmac, M., & Braunbeck, T. (2000). Isolated hepatocytes of rainbow trout (Oncorhynchus mykiss) as a tool to discriminate between differently contaminated small river system. Toxicology in Vitro, 14(4), 361–377. https://doi.org/10.1016/S0887-2333(00)00031-X
Sun, Y., Oberley, L.W., & Li, Y. (1988). A simple method for clinical assay of superoxide dismutase. Clinical Chemistry, 34(3), 497-500. https://doi.org/10.1093/clinchem/34.3.497
Taysı, M.R., Kırıcı, M., Kırıcı, M., Ulusal H., Söğüt, B., & Taysı, S. (2021a). The role of nitrosative and oxidative stress in rainbow trout (Oncorhynchus mykiss) liver tissue applied mercury chloride (HgCl2). Ege Journal of Fisheries and Aquatic Sciences, 38(3), 269 273. https://doi.org/10.12714/egejfas.38.3.02
Taysı, M.R., Kırıcı, M., Kırıcı, M., Söğüt, B., Bozdayı, M. A., Tarakçıoğlu, M., & Taysı, S. (2021b). Effect of mercury chloride on both nitrosative and oxidative stress in the gill tissue of rainbow trout (Oncorhynchus mykiss). KSU Journal of Agriculture and Nature, 24(5), 957-962. https://doi.org/10.18016/ ksutarimdoga.vi.763934.
Teimori, A., Esmaeili, R.H., & Ansari, H.T. (2011). Micro-structure consideration of the adhesive organ in Doctor Fish, Garra rufa (Teleostei; Cyprinidae) from the Persian Gulf Basin. Turkish Journal of Fisheries and Aquatic Sciences, 11, 407-411. https://doi.org/10.4194/1303-2712-v11_3_10
Topal, A., Atamanalp, M., Oruc, E., Kırıcı, M., & Kocaman, E.M. (2014). Apoptotic effects and glucose-6-phosphate dehydrogenase responses in liver and gill tissues of rainbow trout treated with chlorpyrifos. Tissue and Cell, 46(5), 490-496. https://doi.org/10.1016/j.cbi.2007.01.018
Verlecar, X.N., Jena, K.B., & Chainy, G.B.N. (2007). Biochemical markers of oxidative stress in Perna viridis exposed to mercury and temperature. Chemico Biological Interactions, 167(3), 219 226. https://doi.org/10.1016/j.cbi.2007.01.018
Wong, C.K.C., Yeung, H.Y., Cheung, R.Y.H., Yung, K.K.L., & Wong, M.H. (2000). Ecotoxicological assessment of persistent organic and heavy metal contamination in Hong Kong coastal sediment. Archives of Environmental Contamination and Toxicology, 38, 486–495. https://doi.org/10.1007/s002449910064
Yonar, S.M., Sakin, F., Yonar, M.E., Ispir, Ü., & Kırıcı, M. (2011). Oxidative stress biomarkers of exposure to deltamethrin in rainbow trout fry (Oncorhynchus mykiss). Fresenius Environmental Bulletin, 20(8), 1931-1935.
Yonar, M.E., Kırıcı, M., & İspir, Ü. (2012). The investigation of changes in lipid peroxidation and some antioxidant parameters in fry rainbow trout (Oncorhynchus mykiss) exposed to linuron. Fırat University Journal of Science, 24(2), 111-116.
Yonar, M.E., Ispir, U., Yonar, S.M., & Kırıcı, M. (2016). Effect of copper sulphate on the antioxidant parameters in the rainbow trout fry, Oncorhynchus mykiss. Cellular and Molecular Biology (Noisy-le-grand), 62(6), 55-58. https://doi.org/10.14715/cmb/2016.62.6.10

Details

Primary Language

English

Subjects

Fish Biology

Journal Section

Research Article

Authors

Muammer Kırıcı ^*
0000-0003-1888-4388
Türkiye

Nurgül Sen Özdemir
0000-0001-6070-9765
Türkiye

Fatma Caf
0000-0002-0363-4848
Türkiye

Mustafa Koyun
0000-0002-9047-0430
Türkiye

Early Pub Date

September 3, 2023

Publication Date

September 15, 2023

Submission Date

March 6, 2023

Acceptance Date

July 10, 2023

Published in Issue

Year 1970 Volume: 40 Number: 3

DOI

https://doi.org/10.12714/egejfas.40.3.03

IZ

https://izlik.org/JA73PL79PS

Cite

RIS / Bibtex

APA

Kırıcı, M., Sen Özdemir, N., Caf, F., & Koyun, M. (2023). Seasonal changes in antioxidant enzyme activities of Garra rufa (Heckel, 1843) in Göynük Stream (Bingöl, Türkiye). Ege Journal of Fisheries and Aquatic Sciences, 40(3), 174-181. https://doi.org/10.12714/egejfas.40.3.03

Seasonal changes in antioxidant enzyme activities of Garra rufa (Heckel, 1843) in Göynük Stream (Bingöl, Türkiye)

Göynük Çayı'nda (Bingöl, Türkiye) Garra rufa'nın (Heckel, 1843) antioksidan enzim aktivitelerindeki mevsimsel değişimleri

Öz

Anahtar Kelimeler