Seasonal changes in antioxidant enzyme activities of Garra rufa (Heckel, 1843) in Göynük Stream (Bingöl, Türkiye)

Muammer Kırıcı; Nurgül Sen Özdemir; Fatma Caf; Mustafa Koyun

doi:10.12714/egejfas.40.3.03

TR EN

Göynük Çayı'nda (Bingöl, Türkiye) Garra rufa'nın (Heckel, 1843) antioksidan enzim aktivitelerindeki mevsimsel değişimleri

Öz

Bu çalışmada, Murat Nehri’nin en önemli kollarından biri olan Göynük Çayı’ndan yakalanan Garra rufa (Heckel, 1843) balıklarının solungaç, böbrek, kas ve karaciğer dokularında mevsimlere bağlı olarak antioksidan enzim aktivitelerindeki seviyelerindeki değişimler incelendi. Bu amaç doğrultusunda balıklar, her ay düzenli olarak belirlenen iki istasyondan (Garip ve Ilıcalar) yakalanarak laboratuvara getirildi. Dokularda enzim aktiviteleri ve MDA seviyesi spektrofotometrik yöntemlerle belirlendi. Çalışma sonucunda, genel olarak iki istasyon arasında tüm dokularda parametreler arasındaki fark istatistiki olarak önemli bulunmuştur (p < 0.05). Ancak karaciğer dokusunda GR enziminde tüm mevsimlerde istasyonlar arasındaki fark istatistiki olarak önemli olmadığı belirlendi. Bununla beraber, her iki istasyonda da genel olarak mevsimler arasındaki farklılıkların önemli olduğu gözlemlendi. Çalışmada, GR ve G6PD enzim aktiviteleri diğer enzim aktiviteleri ile kıyaslandığında düşükken, CAT ve SOD aktiviteleri ise diğerlerinden yüksektir. Dokular arasında ise kas dokusundaki enzim aktiviteleri, diğer dokulardaki aktivitelerden daha düşüktür.

Anahtar Kelimeler

Seasonal changes in antioxidant enzyme activities of Garra rufa (Heckel, 1843) in Göynük Stream (Bingöl, Türkiye)

Abstract

In this study, seasonal variations of antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glucose 6-phosphate dehydrogenase (G6PD)) and malondialdehyde (MDA) levels in gill, kidney, muscle and liver tissues of Garra rufa (Heckel, 1843) caught from Göynük Stream, one of the most important branch of Murat River, were investigated. Fish samples were caught from two stations (Garip and Ilıcalar), which are determined regularly every month, and brought to the laboratory. The levels of biomarkers in tissues were determined by spectrophotometric methods. It was determined that the difference between the parameters in the studied tissues was statistically significant (P < 0.05) between the two stations, but the difference between the stations in the liver tissue in all seasons in GR enzyme was not statistically significant. However, it was determined that the differences between the seasons were significant in both stations. GR and G6PD enzyme activities were found to be lower than other enzyme activities among the enzyme groups studied, but CAT and SOD enzyme activities were found to be higher than the other enzymes., enzyme activities in muscle tissue are lower than activities in the other tissues among tissues.

Keywords

Supporting Institution

Bingöl Üniversitesi

Project Number

BAP-ZF.2016.00.006

Thanks

This study was supported by the Research Fund (BAP-ZF.2016.00.006) of Bingöl University (Turkey).

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Details

Primary Language

English

Subjects

Fish Biology

Journal Section

Research Article

Authors

Muammer Kırıcı ^*
0000-0003-1888-4388
Türkiye

Nurgül Sen Özdemir
0000-0001-6070-9765
Türkiye

Fatma Caf
0000-0002-0363-4848
Türkiye

Mustafa Koyun
0000-0002-9047-0430
Türkiye

Early Pub Date

September 3, 2023

Publication Date

September 15, 2023

Submission Date

March 6, 2023

Acceptance Date

July 10, 2023

Published in Issue

Year 2023 Volume: 40 Number: 3

DOI

https://doi.org/10.12714/egejfas.40.3.03

IZ

https://izlik.org/JA73PL79PS

Cite

RIS / Bibtex

APA

Kırıcı, M., Sen Özdemir, N., Caf, F., & Koyun, M. (2023). Seasonal changes in antioxidant enzyme activities of Garra rufa (Heckel, 1843) in Göynük Stream (Bingöl, Türkiye). Ege Journal of Fisheries and Aquatic Sciences, 40(3), 174-181. https://doi.org/10.12714/egejfas.40.3.03