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Zebrabalığı (Danio reiro) kalp dokusunda mancozeb’in neden olduğu bazı oksidatif stres parametreleri

Year 2019, Volume 36, Issue 4, 325 - 328, 15.12.2019
https://doi.org/10.12714/egejfas.36.4.02

Abstract



Bu çalışmada, zebra balığı kalp dokusunda mancozeb’in bazı antioksidan enzimler üzerindeki potansiyel toksik etkileri araştırılmıştır. Zebra balığı grupları, 120 saat boyunca farklı dozlarda mancozeb (Grup A: 5 mg L-1 ve Grup B: 7,5 mg L-1) maruz bırakıldı. Bu çalışmada, katalaz (CAT) aktivitesi, malondialdehit (MDA) ve total protein (TP) seviyeleri spektrofotometre ile belirlenmiştir. Elde ettiğimiz sonuçlar CAT aktivitesinin ve MDA seviyesinin tüm deney gruplarında azaldığını gösterdi. Bu çalışmada CAT aktivitesi A grubunda 2,541±0,771 mg L-1, B grubunda ise 2,011±0,201 mg L-1 olarak bulunmuştur. CAT ve MDA aktiviteleri tüm deney gruplarında kontrol grubuna göre azalmıştır. MDA seviyeleri A grubunda 0,025±0,003 mg L-1 ve B grubunda 0,025±0,003 mg L-1 olarak bulunmuştur. TP seviyeleri A grubunda 9,75±1,51 mg L-1, B grubunda ise 10,18±0,32 mg L-1 olarak bulunmuştur. Deney grubunda TP düzeyi kontrol grubuna göre artmıştır. CAT aktivitesi ve MDA seviyelerindeki değişikliklerin deney süresine ve mancozeb dozuna bağlı olduğu belirlenmiştir. Sonuç olarak; mancozeb zebra balığı ve diğer sucul organizmalar için oldukça toksik bir maddedir. 




References

  • Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105,121-126. DOI: 10.1016/S0076-6879(84)05016-3
  • Andreu-Sanchez, O., Paraiba, L.C., Jonsson, C.M., Carrasco, J.M. (2012). Acute toxicity and bioconcentration of fungicide tebuconazole in zebrafish (Danio rerio). Environmental Toxicology, 27:155-168. DOI:10.1002/tox.20618
  • Blahova, J., Plhalova, L., Hostovsky, M., Divisova, L., Dobsikova, R., Mikulikova, I., Stepanova, S. & Z. Svobodova. (2013). Oxidative stress responses in zebrafish Danio rerio after subchronic exposure to atrazine. Food and Chemical Toxicology, 61, 82-85. DOI: 10.1016/j.fct.2013.02.041
  • Bradford, M.M. (1976). A Rapid and Sensitive Method for the Quantitation of Microgram Quantitites of Protein Utilizing the Principle of Protein-dye Binding. Analytical Biochemistry, 72, 248-254. DOI: 10.1016/0003-2697(76)90527-3
  • Clasen, B., Loro, V.L., Murussi, C.R., Tiecher, T.L., Moraes, B., Zanella, R. (2018). Bioaccumulation and oxidative stress caused by pesticides in Cyprinus carpio reared in a rice-fish system. Science of the Total Environment, 626:737-743.
  • EPA (US Environmental Protection Agency) (2005). Mancozeb facts, EPA 738-F-05-XX, (http://www3.epa.gov/pesticides/chem_search/reg_actions/reregistration/fs_PC-014504_1-Sep-05.pdf).
  • Faggio, C., Pagano, M., Alampi, R., Vazzana, I. & Felice, MR. (2016). Cytotoxicity, haemolymphatic parameters, and oxidative stress following exposure to sublethal concentrations of quaternium-15 in Mytilus galloprovincialis. Aquatic Toxicology, 180: 258-265. DOI: 10.1016/j.aquatox.2016.10.010
  • Karaca, M., Varışlı, L., Korkmaz, K., Özaydın, O., perçin, F., Orhan, H. (2014). Organochlorine pesticides and antioxidant enzymes are inversely correlated with liver enzyme gene expression in Cyrinus carpio. Toxicology Letters, 230:198-207. DOI: 10.1016/j.toxlet.2014.02.013
  • Konyalıoğlu, S and Perçin, F. (2017). The comparison of lipid peroxidation, glutathione land antioxidant enzyme activities in blood obtained from captive and wild Northern Bluefin Tuna (Thunnus thynnus L., 1758). Free Radical Biology and Medicine, 108:S18-S107. DOI: 10.1016/j.freeradbiomed.2017.04.196
  • Kumar, S., Nehra, M., Dilbaghi, N., Marrazza, G., Hassan, A.A. & Kim, KH. (2019). Nano-based smart pesticide formulations: Emerging opportunities for agriculture. Journal of Controlled Release, 294,131-153. DOI: 10.1016/j.jconrel.2018.12.012
  • Ledwozyw, A., Michalak, D., Stepien, A. & A. Kadziolka. (1986). The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis. Clinica Chimica Acta, 155, 275- 83. DOI: 10.1016/0009-8981(86)90247-0
  • Maharajan, K., Muthulakshmi, S., Nataraj, B., Ramesh, M. (2018). Toxicity assessment of pyriproxyfen in vertebrate model zebrafish embryos (Danio rerio): A multi biomarker study. Aquatic Toxicology, 196:132-145. DOI: 10.1016/j.aquatox.2018.01.010
  • Mc Collum, C.W., Ducharme, N.A., Bondesson, M., Gustafsson, J.A. (2011). Developmental toxicity Screening in zebrafish. Birth Defects Research. Part-C. Embryo Today Reviews, 93:67-114. DOI: 10.1002/bdrc.20210
  • Miron, D.S., Prettob, A., Crestani, M., Glusczak, L., Schetinger, M.R., Loro, V.L. & Morsch, V.M. (2008). Biochemical effects of clomazone herbicide on piava (L. obtusidens). Chemosphere, 74, 1–5. DOI: 10.1016/j.chemosphere.2008.09.070
  • Moraes, B.S., Clasen, B., Loro, V.L., Pretto, A., Toni, C., De Avila, L.A., Marchesan, E., De Oliveira Machado, S.L., Zanella, R. & G.B. Reimche. (2011). Toxicological responses of Cyprinus carpio after exposure to a commercial herbicide containing imazethapyr and imazapic. Ecotoxicology and Environmental Safety, 74, 328-335. DOI: 10.1016/j.ecoenv.2009.05.013
  • Morales, A.E., Pèrez-Jimènez, A., Hidalgo, M.C., Abellán, E. & Gabriel C.G. (2004). Oxidative stress and antioxidant defenses after prolonged starvation in Dentex dentex liver. Comporative Biochemistry and Physiology C, 139(1-3),153-161. DOI: 10.1016/j.cca.2004.10.008
  • Pascual, P., Pedrajas, J., Toribio, F., López-Barea, J., & Peinado, J. (2003). Effect of food deprivation on oxidative stress biomarkers in fish (Sparus aurata). Chemico-Biological Interactions, 145(2), 191-199. DOI: 10.1016/s0009-2797(03)00002-4
  • Percin, F., Sogut, O. (2010). Trace elements in heart tissue of wild and fattened bluefin tuna (Thunnus thynnus L. 1758) in the Turkish part of the Eastern Mediterranean. Journal of Food Agriculture and Environment. 8 (3) 1184-1187.
  • Plhalova, L., Blahova, J., Divisova, L., Enevova, V., Casuscelli di Tocco, F., Faggio, C., Tichy, F., Vecerek, V. & Z. Svobodova. (2017): The effects of subchronic exposure to NeemAzal T/S on zebrafish (Danio rerio), Chemistry and Ecology, DOI: 10.1080/02757540.2017.1420176
  • Qiu, L., Jia, K., Huang, L., Liano, X., Guo, X., Lu, H. (2019). Hepatotoxicity of tricylazole in zebrafish (Danio rerio). Chemosphere, 232:171-179. DOI: 10.1016/j.chemosphere.2019.05.159
  • Ribas, L. and Pifeffer, F. (2013). The zebrafish (Danio rerio) as a model organism, with emphasis on applications for finfish aquaculture research. Reviews in Aquaculture, 5:1-32. DOI: 10.1111/raq.12041
  • San, A.T. & Yonar, M.E. (2017). Determination of oxidative stress in scaly carp (Cyprinus carpio Linnaeus, 1758) exposed to deltamethrin in different water temperature. Ege Journal of Fisheries and Aquatic Sciences, 34(3), 281-286. DOI: 10.12714/egejfas.2017.34.3.06
  • Tabassum, H., Dawood, A.Q., Sharma, P., Khan, J., Raisuddin, S. & S. Parvez. (2016). Multiorgan toxicological impact of fungicide propiconazole on biochemical and histological profile of freshwater fish Channa punctata Bloch. Ecological Indicators, 63, 359-365. DOI: 10.1016/j.ecolind.2015.11.052
  • Tsaboula, A., Papadakis, E.N., Vyrzas, Z., Kotopoulou, A., Kintzikoglou, K. & E. Papadopoulou-Mourkidou. (2016). Environmental and human risk hierarchy of pesticides: A prioritization method, based on monitoring, hazard assessment and environmental fate. Environment International, 91, 78-93. DOI: 10.1016/j.envint.2016.02.008
  • Vieira, C.E.D., Perez, M.R., Acayaba, R.D., Raimundo, C.C.M., Martinez, C.B.R. (2018). DNA damage and oxidative stress induced by imidacloprid exposure in different tissues of the Neotropical fish Prochilodus lineatus. Chemosphere, 195:125-134. DOI: 10.1016/j.chemosphere.2017.12.077
  • Wagner, D., Brühl, M., Veith, P.P. & S. Lötters. (2014). Evaluating the risk of pesticide exposure for amphibian species listed in Annex II of the European Union Habitats Directive. Biological Conservation, 176, 64–70. DOI: 10.1016/j.biocon.2014.05.014
  • Zengin, H. (2018). Oxidative stress and antioxidant defenses after starvation in Oncorhynchus mykiss (Walbaum,1792) larvae reached free swimming stage. Ege Journal of Fisheries and Aquatic Sciences, 35(4), 387-396. DOI: 10.12714/egejfas.2018.35.4.04
  • Zhu, X.Y., Xia, B., Wu, Y.Y., Yang, H., Li, C.Q., Li, P. (2019). Fenobucarb induces heart failure and cerebral hemorrhage in zebrafish. Aquatic Toxicology, 209:34-41. DOI: 10.1016/j.aquatox.2018.12.020

Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb

Year 2019, Volume 36, Issue 4, 325 - 328, 15.12.2019
https://doi.org/10.12714/egejfas.36.4.02

Abstract



The potential toxic effects of mancozeb exposure on some antioxidant enzyme were investigated on heart tissue of zebrafish in this study. Zebrafish groups were exposed to different doses of mancozeb (Group A: 5 mgL-1 and Group B: 7.5 mgL-1) for 120 hours. In this study, catalase (CAT) activity, malondialdehyde (MDA) level and total protein (TP) level were determined with spectrophotometer. Our results showed that CAT activity was found 2,541±0,771 mg L-1 in A group and 2,011±0,201 mg L-1 in B group in this study. CAT and MDA activity levels decreased in the experiment group according to control group. MDA levels were found 0,025±0,003 mg L-1 in A group and 0,025±0,003 mg L-1 in B group. TP levels were found 9,75±1,51 mg L-1 in A group and 10,18±0,32 mg L-1 in B group. TP levels increased in the all experiment groups according to control group. We observed that the changes in the CAT activity and MDA levels were time and as well as mancozeb dose dependent. As a result mancozeb is a very toxic substance for zebrafish and other aquatic organisms.   




References

  • Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105,121-126. DOI: 10.1016/S0076-6879(84)05016-3
  • Andreu-Sanchez, O., Paraiba, L.C., Jonsson, C.M., Carrasco, J.M. (2012). Acute toxicity and bioconcentration of fungicide tebuconazole in zebrafish (Danio rerio). Environmental Toxicology, 27:155-168. DOI:10.1002/tox.20618
  • Blahova, J., Plhalova, L., Hostovsky, M., Divisova, L., Dobsikova, R., Mikulikova, I., Stepanova, S. & Z. Svobodova. (2013). Oxidative stress responses in zebrafish Danio rerio after subchronic exposure to atrazine. Food and Chemical Toxicology, 61, 82-85. DOI: 10.1016/j.fct.2013.02.041
  • Bradford, M.M. (1976). A Rapid and Sensitive Method for the Quantitation of Microgram Quantitites of Protein Utilizing the Principle of Protein-dye Binding. Analytical Biochemistry, 72, 248-254. DOI: 10.1016/0003-2697(76)90527-3
  • Clasen, B., Loro, V.L., Murussi, C.R., Tiecher, T.L., Moraes, B., Zanella, R. (2018). Bioaccumulation and oxidative stress caused by pesticides in Cyprinus carpio reared in a rice-fish system. Science of the Total Environment, 626:737-743.
  • EPA (US Environmental Protection Agency) (2005). Mancozeb facts, EPA 738-F-05-XX, (http://www3.epa.gov/pesticides/chem_search/reg_actions/reregistration/fs_PC-014504_1-Sep-05.pdf).
  • Faggio, C., Pagano, M., Alampi, R., Vazzana, I. & Felice, MR. (2016). Cytotoxicity, haemolymphatic parameters, and oxidative stress following exposure to sublethal concentrations of quaternium-15 in Mytilus galloprovincialis. Aquatic Toxicology, 180: 258-265. DOI: 10.1016/j.aquatox.2016.10.010
  • Karaca, M., Varışlı, L., Korkmaz, K., Özaydın, O., perçin, F., Orhan, H. (2014). Organochlorine pesticides and antioxidant enzymes are inversely correlated with liver enzyme gene expression in Cyrinus carpio. Toxicology Letters, 230:198-207. DOI: 10.1016/j.toxlet.2014.02.013
  • Konyalıoğlu, S and Perçin, F. (2017). The comparison of lipid peroxidation, glutathione land antioxidant enzyme activities in blood obtained from captive and wild Northern Bluefin Tuna (Thunnus thynnus L., 1758). Free Radical Biology and Medicine, 108:S18-S107. DOI: 10.1016/j.freeradbiomed.2017.04.196
  • Kumar, S., Nehra, M., Dilbaghi, N., Marrazza, G., Hassan, A.A. & Kim, KH. (2019). Nano-based smart pesticide formulations: Emerging opportunities for agriculture. Journal of Controlled Release, 294,131-153. DOI: 10.1016/j.jconrel.2018.12.012
  • Ledwozyw, A., Michalak, D., Stepien, A. & A. Kadziolka. (1986). The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis. Clinica Chimica Acta, 155, 275- 83. DOI: 10.1016/0009-8981(86)90247-0
  • Maharajan, K., Muthulakshmi, S., Nataraj, B., Ramesh, M. (2018). Toxicity assessment of pyriproxyfen in vertebrate model zebrafish embryos (Danio rerio): A multi biomarker study. Aquatic Toxicology, 196:132-145. DOI: 10.1016/j.aquatox.2018.01.010
  • Mc Collum, C.W., Ducharme, N.A., Bondesson, M., Gustafsson, J.A. (2011). Developmental toxicity Screening in zebrafish. Birth Defects Research. Part-C. Embryo Today Reviews, 93:67-114. DOI: 10.1002/bdrc.20210
  • Miron, D.S., Prettob, A., Crestani, M., Glusczak, L., Schetinger, M.R., Loro, V.L. & Morsch, V.M. (2008). Biochemical effects of clomazone herbicide on piava (L. obtusidens). Chemosphere, 74, 1–5. DOI: 10.1016/j.chemosphere.2008.09.070
  • Moraes, B.S., Clasen, B., Loro, V.L., Pretto, A., Toni, C., De Avila, L.A., Marchesan, E., De Oliveira Machado, S.L., Zanella, R. & G.B. Reimche. (2011). Toxicological responses of Cyprinus carpio after exposure to a commercial herbicide containing imazethapyr and imazapic. Ecotoxicology and Environmental Safety, 74, 328-335. DOI: 10.1016/j.ecoenv.2009.05.013
  • Morales, A.E., Pèrez-Jimènez, A., Hidalgo, M.C., Abellán, E. & Gabriel C.G. (2004). Oxidative stress and antioxidant defenses after prolonged starvation in Dentex dentex liver. Comporative Biochemistry and Physiology C, 139(1-3),153-161. DOI: 10.1016/j.cca.2004.10.008
  • Pascual, P., Pedrajas, J., Toribio, F., López-Barea, J., & Peinado, J. (2003). Effect of food deprivation on oxidative stress biomarkers in fish (Sparus aurata). Chemico-Biological Interactions, 145(2), 191-199. DOI: 10.1016/s0009-2797(03)00002-4
  • Percin, F., Sogut, O. (2010). Trace elements in heart tissue of wild and fattened bluefin tuna (Thunnus thynnus L. 1758) in the Turkish part of the Eastern Mediterranean. Journal of Food Agriculture and Environment. 8 (3) 1184-1187.
  • Plhalova, L., Blahova, J., Divisova, L., Enevova, V., Casuscelli di Tocco, F., Faggio, C., Tichy, F., Vecerek, V. & Z. Svobodova. (2017): The effects of subchronic exposure to NeemAzal T/S on zebrafish (Danio rerio), Chemistry and Ecology, DOI: 10.1080/02757540.2017.1420176
  • Qiu, L., Jia, K., Huang, L., Liano, X., Guo, X., Lu, H. (2019). Hepatotoxicity of tricylazole in zebrafish (Danio rerio). Chemosphere, 232:171-179. DOI: 10.1016/j.chemosphere.2019.05.159
  • Ribas, L. and Pifeffer, F. (2013). The zebrafish (Danio rerio) as a model organism, with emphasis on applications for finfish aquaculture research. Reviews in Aquaculture, 5:1-32. DOI: 10.1111/raq.12041
  • San, A.T. & Yonar, M.E. (2017). Determination of oxidative stress in scaly carp (Cyprinus carpio Linnaeus, 1758) exposed to deltamethrin in different water temperature. Ege Journal of Fisheries and Aquatic Sciences, 34(3), 281-286. DOI: 10.12714/egejfas.2017.34.3.06
  • Tabassum, H., Dawood, A.Q., Sharma, P., Khan, J., Raisuddin, S. & S. Parvez. (2016). Multiorgan toxicological impact of fungicide propiconazole on biochemical and histological profile of freshwater fish Channa punctata Bloch. Ecological Indicators, 63, 359-365. DOI: 10.1016/j.ecolind.2015.11.052
  • Tsaboula, A., Papadakis, E.N., Vyrzas, Z., Kotopoulou, A., Kintzikoglou, K. & E. Papadopoulou-Mourkidou. (2016). Environmental and human risk hierarchy of pesticides: A prioritization method, based on monitoring, hazard assessment and environmental fate. Environment International, 91, 78-93. DOI: 10.1016/j.envint.2016.02.008
  • Vieira, C.E.D., Perez, M.R., Acayaba, R.D., Raimundo, C.C.M., Martinez, C.B.R. (2018). DNA damage and oxidative stress induced by imidacloprid exposure in different tissues of the Neotropical fish Prochilodus lineatus. Chemosphere, 195:125-134. DOI: 10.1016/j.chemosphere.2017.12.077
  • Wagner, D., Brühl, M., Veith, P.P. & S. Lötters. (2014). Evaluating the risk of pesticide exposure for amphibian species listed in Annex II of the European Union Habitats Directive. Biological Conservation, 176, 64–70. DOI: 10.1016/j.biocon.2014.05.014
  • Zengin, H. (2018). Oxidative stress and antioxidant defenses after starvation in Oncorhynchus mykiss (Walbaum,1792) larvae reached free swimming stage. Ege Journal of Fisheries and Aquatic Sciences, 35(4), 387-396. DOI: 10.12714/egejfas.2018.35.4.04
  • Zhu, X.Y., Xia, B., Wu, Y.Y., Yang, H., Li, C.Q., Li, P. (2019). Fenobucarb induces heart failure and cerebral hemorrhage in zebrafish. Aquatic Toxicology, 209:34-41. DOI: 10.1016/j.aquatox.2018.12.020

Details

Primary Language English
Subjects Science
Journal Section Articles
Authors

Figen Esin KAYHAN (Primary Author)
MARMARA ÜNİVERSİTESİ
0000-0001-7754-1356
Türkiye


Güllü KAYMAK
MARMARA ÜNİVERSİTESİ
0000-0001-6309-0208
Türkiye


Harika Eylül ESMER DURUEL
MARMARA ÜNİVERSİTESİ
0000-0002-0792-2062


Şeyma KIZILKAYA This is me
MARMARA ÜNİVERSİTESİ
0000-0001-8065-217X
Türkiye

Publication Date December 15, 2019
Application Date February 5, 2019
Acceptance Date July 2, 2019
Published in Issue Year 2019, Volume 36, Issue 4

Cite

APA Kayhan, F. E. , Kaymak, G. , Esmer Duruel, H. E. & Kızılkaya, Ş. (2019). Some oxidative stress parameters in heart tissue of Zebrafish (Danio reiro) caused by mancozeb . Ege Journal of Fisheries and Aquatic Sciences , 36 (4) , 325-328 . DOI: 10.12714/egejfas.36.4.02