Cıva toksisitesi üzerine selenyumun koruyucu rolünün Oreochromis niloticus (Linnaeus, 1758)’ta katalaz, süperoksit dismutaz ve malondialdehit parametreleri ile değerlendirilmesi

Özgür Fırat; Özlem Kaya

doi:10.12714/egejfas.2019.36.3.05

EN TR

Evaluation of protective role of selenium on mercury toxicity by superoxide dismutase, catalase and malondialdehyde parameters in Oreochromis niloticus (Linnaeus, 1758)

Abstract

In this research, to determine toxicity of mercury (Hg) and whether selenium (Se) has any role in protection of this toxicity, it was investigated the alterations in oxidative stress parameters in tissues of Oreochromis niloticus. For this purpose, fish were exposed to 0.01 and 0.05 ppm Hg and 0.01 ppm Hg+0.01 ppm Se and 0.05 ppm Hg+0.05 ppm Se for 4 and 21 days and activities of superoxide dismutase (SOD) and catalase (CAT) and levels of malondialdehyde (MDA) in gill and liver were determined. It was observed significant changes in all analyzed parameters due to tissue, medium concentration and exposure period in the exposure of mercury alone and Hg+Se mixtures. In Hg alone, and in combination with Se especially in their higher medium concentrations, activities of SOD and CAT in the gill and liver significantly increased at 4 days (P˂0.05), while they significantly decreased at 21 days (P˂0.05). In the exposure of Hg and Hg+Se and in the both tissues, it not determined significant alteration in the MDA levels at 4 days (P˃0.05), while they elevated in their higher concentrations at 21 days (P˂0.05). In conclusion, it was determined the increases or decreases in activities of SOD and CAT and levels of MDA in O. niloticus were higher in the Hg alone than Hg+Se mixtures and selenium has a protective effect on oxidative toxicity of mercury

Keywords

Cıva toksisitesi üzerine selenyumun koruyucu rolünün Oreochromis niloticus (Linnaeus, 1758)’ta katalaz, süperoksit dismutaz ve malondialdehit parametreleri ile değerlendirilmesi

Öz

Bu araştırmada cıva (Hg) toksisitesini ve bu toksisite üzerine selenyumun (Se) koruyucu rolünü belirlemek için Oreochromis niloticus’un dokularındaki oksidatif stres parametreleri çalışılmıştır. Bu amaçla balıklar 4 ve 21 günlük sürelerle 0.01 ve 0.05 ppm Hg ile 0.01+0.01 ve 0.05+0.05 ppm Hg+Se karışımlarının etkisine bırakılmış ve solungaç ve karaciğer süperoksit dismutaz (SOD) ve katalaz (CAT) aktiviteleri ile malondialdehit (MDA) düzeyleri belirlenmiştir. Hg’nin tek başına ve Se ile birlikte etkisinde incelenen oksidatif stres parametrelerinde dokuya, ortam derişimine ve etki süresine bağlı olarak önemli değişimlerin meydana geldiği belirlenmiştir. Cıvanın tek başına ve cıva+selenyum karışımının etkisinde özellikle de yüksek ortam derişimlerinde solungaç ve karaciğer SOD ve CAT aktiviteleri, 4 günlük süre sonunda anlamlı bir artış gösterirken (P˂0.05); 21 günlük süre sonunda ise anlamlı bir azalma göstermiştir (P˂0.05). Hg ve Hg+Se karışımının etkisinde her iki dokuda 4 günlük süre sonunda tüm ortam derişimlerinde önemli bir değişim göstermeyen (P˃0.05) MDA düzeyinin, 21 günlük süre sonunda yüksek ortam derişimlerinde anlamlı bir artış gösterdiği saptanmıştır (P˂0.05). Sonuç olarak O. niloticus’ta belirlenen SOD ve CAT aktiviteleri ile MDA düzeylerindeki artış veya azalışların cıva+selenyum karışımına oranla cıvanın tek başına etkisinde daha fazla olduğu ve selenyumun cıvanın oksidatif toksisitesi üzerine koruyucu bir etkiye sahip olduğu belirlenmiştir.

Anahtar Kelimeler

Supporting Institution

Adıyaman Üniversitesi

Project Number

FEFYL2014-0010

Thanks

Bu çalışma yüksek lisans tez çalışması olup FEFYL2014-0010 nolu Adıyaman Üniversitesi (ADYÜ) Bilimsel Araştırma Projesi ile yürütülmüş olup ADYÜ Bilimsel Araştırma Projeleri Koordinasyon Biriminin değerli yöneticilerine ve çalışanlarına teşekkür ederiz.

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Details

Primary Language

Turkish

Subjects

-

Journal Section

Research Article

Authors

Özgür Fırat ^*
0000-0002-9683-8945
Türkiye

Özlem Kaya
0000-0002-2885-1865
Türkiye

Publication Date

September 15, 2019

Submission Date

January 25, 2019

Acceptance Date

June 17, 2019

Published in Issue

Year 1970 Volume: 36 Number: 3

DOI

https://doi.org/10.12714/egejfas.2019.36.3.05

IZ

https://izlik.org/JA82FG72TH

Cite

RIS / Bibtex

APA

Fırat, Ö., & Kaya, Ö. (2019). Cıva toksisitesi üzerine selenyumun koruyucu rolünün Oreochromis niloticus (Linnaeus, 1758)’ta katalaz, süperoksit dismutaz ve malondialdehit parametreleri ile değerlendirilmesi. Ege Journal of Fisheries and Aquatic Sciences, 36(3), 245-253. https://doi.org/10.12714/egejfas.2019.36.3.05

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