Evaluation of DNA damage by Comet Assay in populations of endemic Beyşehir frog Pelophylax caralitanus (Arıkan,1988)

Uğur Cengiz Erişmiş

doi:10.12714/egejfas.2019.36.2.02

TR EN

Endemik Beyşehir kurbağası Pelophylax caralitanus (Arıkan,1988) populasyonlarında Comet Analizi ile DNA hasarın değerlendirilmesi

Öz

Bu çalışmada, DNA Comet Assay tekniği kullanılarak, farklı antropojenik kirliliğe sahip Karamık, Eber and Beyşehir Göllerinde yaşayan endemik Beyşehir kurbağası (Pelophylax caralitanus) üç populasyonunda DNA hasar düzeyleri değerlendirilmiştir. Analizler için kurbağa eritrosit hücreleri kullanıldı ve uygun biyotoplardan toplanılan 7 ergin bireyin, her birinden minimum 100 hücre ile analiz edilmiştir. Sonuçlar DNA comet indeksinde kontamine olmuş zonda [(Eber Gölü = Idc=0.22±0.015), (p<0.05)] artış olduğu gösterirken, temiz zonda, hücrelerin çoğunluğunun hasarsız DNA içerdiğini (Idc = 0.08 ± 0.001) göstermiştir. Elde edilen veriler, endemik Beyşehir kurbağasının ekosistemler üzerindeki antropojenik etkinin bayrak türü göstergesi olarak kullanılma olasılığını göstermektedir.

Anahtar Kelimeler

Pelophylax caralitanus,endemik Beyşehir kurbağası,DNA hasarı,comet assay,antropojenik etki

Supporting Institution

TÜBİTAK - Afyon Kocatepe University

Project Number

KBAG 113Z139 - 16.FENED.07

Thanks

This work was supported by the Scientific and Technological Research Council of Turkey (TÜBİTAK), Grant No:KBAG 113Z139 and Afyon Kocatepe University Research Funds Grant No:16.FENED.07

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Uğur Cengiz Erişmiş ^*
0000-0002-1288-3088
Türkiye

Publication Date

June 15, 2019

Submission Date

October 30, 2018

Acceptance Date

January 23, 2019

Published in Issue

Year 1970 Volume: 36 Number: 2

DOI

https://doi.org/10.12714/egejfas.2019.36.2.02

IZ

https://izlik.org/JA94MN96UU

Cite

RIS / Bibtex

APA

Erişmiş, U. C. (2019). Evaluation of DNA damage by Comet Assay in populations of endemic Beyşehir frog Pelophylax caralitanus (Arıkan,1988). Ege Journal of Fisheries and Aquatic Sciences, 36(2), 109-114. https://doi.org/10.12714/egejfas.2019.36.2.02

Evaluation of DNA damage by Comet Assay in populations of endemic Beyşehir frog Pelophylax caralitanus (Arıkan,1988)

Endemik Beyşehir kurbağası Pelophylax caralitanus (Arıkan,1988) populasyonlarında Comet Analizi ile DNA hasarın değerlendirilmesi

Öz

Anahtar Kelimeler