Year 2019, Volume 36 , Issue 2, Pages 109 - 114 2019-06-15

Evaluation of DNA damage by Comet Assay in populations of endemic Beyşehir frog Pelophylax caralitanus (Arıkan,1988)
Endemik Beyşehir kurbağası Pelophylax caralitanus (Arıkan,1988) populasyonlarında Comet Analizi ile DNA hasarın değerlendirilmesi

Uğur Cengiz ERİŞMİŞ [1]




In study this, the level of DNA damage in three populations of endemic Beyşehir frog (Pelophylax caralitanus) inhabiting on Lakes of Karamık, Eber and Beyşehir with different anthropogenic pollution was assessed by using the DNA Comet Assay technique. Frog erythrocytes cells were used for the analysis. Seven adult individuals were collected from each biotope containing minimum 100 cells were analysed. While the results showing a significant increase in the DNA comet index in the contaminated zone [(Eber Lake= Idc=0.22±0.015), (p<0.05)], the majority of the cells did not contain any DNA damage in the clean zone (Karamık Lake, Idc=0.08±0.001). The obtained data demonstrated t that the endemic Beyşehir frog could serve as a useful flag species in an indicator of anthropogenic impact on ecosystems.






Bu çalışmada, DNA Comet Assay tekniği kullanılarak, farklı antropojenik kirliliğe sahip Karamık, Eber and Beyşehir Göllerinde yaşayan endemik Beyşehir kurbağası (Pelophylax caralitanus) üç populasyonunda DNA hasar düzeyleri değerlendirilmiştir. Analizler için kurbağa eritrosit hücreleri kullanıldı ve uygun biyotoplardan toplanılan 7 ergin bireyin, her birinden minimum 100 hücre ile analiz edilmiştir. Sonuçlar DNA comet indeksinde kontamine olmuş zonda [(Eber Gölü = Idc=0.22±0.015), (p<0.05)] artış olduğu gösterirken, temiz zonda, hücrelerin çoğunluğunun hasarsız DNA içerdiğini (Idc = 0.08 ± 0.001) göstermiştir. Elde edilen veriler, endemik Beyşehir kurbağasının ekosistemler üzerindeki antropojenik etkinin bayrak türü göstergesi olarak kullanılma olasılığını göstermektedir.




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Primary Language en
Subjects Science
Journal Section Articles
Authors

Orcid: 0000-0002-1288-3088
Author: Uğur Cengiz ERİŞMİŞ (Primary Author)
Institution: Afyon Kocatepe University, Faculty of Sciences and Literatures, Moleculer Biology & Genetics Department
Country: Turkey


Supporting Institution TÜBİTAK - Afyon Kocatepe University
Project Number KBAG 113Z139 - 16.FENED.07
Thanks This work was supported by the Scientific and Technological Research Council of Turkey (TÜBİTAK), Grant No:KBAG 113Z139 and Afyon Kocatepe University Research Funds Grant No:16.FENED.07
Dates

Application Date : October 30, 2018
Acceptance Date : January 23, 2019
Publication Date : June 15, 2019

APA Eri̇şmi̇ş, U . (2019). Evaluation of DNA damage by Comet Assay in populations of endemic Beyşehir frog Pelophylax caralitanus (Arıkan,1988) . Ege Journal of Fisheries and Aquatic Sciences , 36 (2) , 109-114 . DOI: 10.12714/egejfas.2019.36.2.02