Abstract
Bu araştırmada Chlorella vulgaris kültürlerinde farklı ortamların
ve farklı ışık yolu uzunluğuna sahip olan panel fotobiyoreaktörlerin etkileri
incelenmiştir. Jaworski besin ortamında hızlı bir hücre artışı tespit edilmiştir
(60,8 x 106 hücre mL-1). F/2 besin ortamında ise 11.
günde hücre sayısı 1,37 x 106 hücre mL-1 olarak kayıt
edilmiştir. Jaworski ve F/2 besin ortamlarında yetişen kültürlerin kuru ağırlık
değeri 11. gün sonunda 0,46
gL-1, 0,13 gL-1 olarak ölçülmüştür. Cam panel biyoreaktör
denemelerinde en yüksek hücre sayısına 31. günde 1 cm ışık yolu uzunluğuna
sahip biyoreaktörde ulaşılmıştır (230 x 106 hücre mL-1).
Fakat 3 cm ve 5cm ışık yoluna sahip biyoreaktörlerde ise en yüksek hücre sayısı
olduğu değer 90 x 106 hücre mL-1 olarak tespit edilmiştir.
1, 3 ve 5 cm ışık yolu uzunluğuna sahip biyoreaktörlerde maksimum kuru ağırlık
değerleri ise sırasıyla 3,6 gL-1, 2,8 gL-1 ve 2,4 gL-1
olarak tespit edilmiştir. Panellerde yapılan Chlorella vulgaris
kültürlerin klorofil a değerleri zamanla düştüğü tespit edilmiştir. Aynı zamanda yapılan denemede büyüme oranları
ile klorofil a değerlerinin ters orantılı olduğu gözlenmiştir.
References
- Pulz, O. (2001). Photobioreaktors; Production Systems For Phototrophic Microorganisms. Igv Institute For Cereal Processing, Arthur- Shcheunerd-Alle, Bergholz- Rehbrücke, 40/41: Germany.
- Richmond, A. (1986). Handbook Of Microalgal Mass Culture, FL: CRC Press, Boca Raton.
- Sánchez, M. D., Mantell, C., Rodrıguez, M., de La Ossa, E. M., Lubián, L. M., & Montero, O. (2005). Supercritical fluid extraction of carotenoids and chlorophyll a from Nannochloropsis gaditana, Journal of Food Engineering, 66(2), 245-251, DOI:10.1016/j.jfoodeng.2004.03.021
- Sukenik, A. (1991). Ecophysiological considerations in the optimization of eicosapentaenoic acid production by Nannochloropsis sp. (Eustigmatophyceae). Bioresource Technology, 35:263–269. DOI: 10.1016/0960-8524(91)90123-2
- Tompkins, J., Deville, M. M., Day, J. G. & Turner, M.F. (1995). The culture collection of algae and protozoa. Ambleside: Institute of Freshwater Ecology, Culture collection of algae and protozoa. Catalogue of strains; p. 204.
- Zijffers, JWF., Janssen, M., Tramper, J. & Wijffels, RH. (2008). Design process of an area-efficient photobioreactor, Marine Biotechnology, 10: 404–415. DOI: 10.1007/s10126-007-9077-2
- Zou, N. & Richmond, A. (1999). Effect of light- path length in outdoor flate plate reactors on output rate of cell mass and of EPA in Nannochloropsis sp, Journal of Biotechnology, 70: 351-356, DOI: 10.1016/S0168-1656(99)00087-5
- Borowitzka, M.A. (1992). Algal biotechnology products and poresses matching science and econimics, Journal of Applied Phycology 4: 267- 279 DOI:10.1007/BF02161212
- Brown, M.R., Jeffrey, S.W. & Garland, C.D. (1989). Nutritional aspects of microalgae used in mariculture: a literature review. CSIRO Marine Laboratories press 0725-4598, no: 205.
- Cohen, Z., Vonshak, A. & Richmond, A. (1988). Effect of environmental conditions on fatty acid composition of the red alga Porphyridium cruentum: correlation to growth rate. Journal of Phycology, 24(3), 328-332, DOI: 10.1111/j.1529-8817.1988.tb04474.x
- Degen, J., Uebele, A., Retze, A., Schmidt-Staigar, U. & Trosch, W. (2001). A novel airlift photobioreactor with baffles for improved light utilization through the flashing light effect, Journal of Biotechnology, 92:89–94p DOI:10.1016/S0168-1656(01)00350-9
- Durmaz, Y. (2000). Chlorella sp’ nin ince cam panel biyoreaktörlerde üretiminde ışığın etkisi üzerine bir araştırma, Ege Üniversitesi Fen Bilimleri Enstitüsü Yüksek Lisans Tezi, Bornova İzmir.
- Guillard, R.R.L. (1975). Culture of phytoplankton for feeding marine invertebrate. In: Smith, W.L., Chanley, M.H. (Eds.), Culture of Marine Invertebrates Animals. Plenum, New York.
The effect of different growth medium and different light path length on the culture of Chlorella vulgaris
Abstract
In this study, the effects of panel
photobioreactors with different nutrient mediums and different light path
lengths on Chlorella vulgaris cultures were investigated. Rapid cell
growth was detected in the Jaworski nutrient medium (60.8 x 106
cells mL-1). In the F / 2 nutrient medium, the cell number was
recorded as 1.37 x 106 cells mL-1 on the 11th
day. Dry weight of cultures grown in Jaworski and F / 2 nutrient media were
measured as 0.46 gL-1 and 0.13 gL-1 at the end of 11
days, respectively. At the experiments in glass panel bioreactor, the highest
cell number was found on the 31st day (230 x 106 cells mL-1)
in a bioreactor with 1 cm light path length. Whereas, the highest cell numbers
was found as 90 x 106 cells mL-1 in glass panel
bioreactors with 3 cm and 5 cm light path length. The maximum dry weights for
bioreactors with 1, 3 and 5 cm light path lengths were determined as 3.6 gL-1,
2.8 gL-1 and 2.4 gL-1, respectively. It was also
determined that chlorophyll a values of Chlorella vulgaris on the panels
bioreactors decreased over time. Moreover, the inversely proportion between
growth rates and the chlorophyll-a values was observed.
References
- Pulz, O. (2001). Photobioreaktors; Production Systems For Phototrophic Microorganisms. Igv Institute For Cereal Processing, Arthur- Shcheunerd-Alle, Bergholz- Rehbrücke, 40/41: Germany.
- Richmond, A. (1986). Handbook Of Microalgal Mass Culture, FL: CRC Press, Boca Raton.
- Sánchez, M. D., Mantell, C., Rodrıguez, M., de La Ossa, E. M., Lubián, L. M., & Montero, O. (2005). Supercritical fluid extraction of carotenoids and chlorophyll a from Nannochloropsis gaditana, Journal of Food Engineering, 66(2), 245-251, DOI:10.1016/j.jfoodeng.2004.03.021
- Sukenik, A. (1991). Ecophysiological considerations in the optimization of eicosapentaenoic acid production by Nannochloropsis sp. (Eustigmatophyceae). Bioresource Technology, 35:263–269. DOI: 10.1016/0960-8524(91)90123-2
- Tompkins, J., Deville, M. M., Day, J. G. & Turner, M.F. (1995). The culture collection of algae and protozoa. Ambleside: Institute of Freshwater Ecology, Culture collection of algae and protozoa. Catalogue of strains; p. 204.
- Zijffers, JWF., Janssen, M., Tramper, J. & Wijffels, RH. (2008). Design process of an area-efficient photobioreactor, Marine Biotechnology, 10: 404–415. DOI: 10.1007/s10126-007-9077-2
- Zou, N. & Richmond, A. (1999). Effect of light- path length in outdoor flate plate reactors on output rate of cell mass and of EPA in Nannochloropsis sp, Journal of Biotechnology, 70: 351-356, DOI: 10.1016/S0168-1656(99)00087-5
- Borowitzka, M.A. (1992). Algal biotechnology products and poresses matching science and econimics, Journal of Applied Phycology 4: 267- 279 DOI:10.1007/BF02161212
- Brown, M.R., Jeffrey, S.W. & Garland, C.D. (1989). Nutritional aspects of microalgae used in mariculture: a literature review. CSIRO Marine Laboratories press 0725-4598, no: 205.
- Cohen, Z., Vonshak, A. & Richmond, A. (1988). Effect of environmental conditions on fatty acid composition of the red alga Porphyridium cruentum: correlation to growth rate. Journal of Phycology, 24(3), 328-332, DOI: 10.1111/j.1529-8817.1988.tb04474.x
- Degen, J., Uebele, A., Retze, A., Schmidt-Staigar, U. & Trosch, W. (2001). A novel airlift photobioreactor with baffles for improved light utilization through the flashing light effect, Journal of Biotechnology, 92:89–94p DOI:10.1016/S0168-1656(01)00350-9
- Durmaz, Y. (2000). Chlorella sp’ nin ince cam panel biyoreaktörlerde üretiminde ışığın etkisi üzerine bir araştırma, Ege Üniversitesi Fen Bilimleri Enstitüsü Yüksek Lisans Tezi, Bornova İzmir.
- Guillard, R.R.L. (1975). Culture of phytoplankton for feeding marine invertebrate. In: Smith, W.L., Chanley, M.H. (Eds.), Culture of Marine Invertebrates Animals. Plenum, New York.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | June 15, 2018 |
| Submission Date | February 1, 2018 |
| Published in Issue | Year 2018Volume: 35 Issue: 2 |