Abstract: : Diazinon (Organophosphorus insecticide) can cause reactive oxidative stresses during metabolism of aquatic organisms. The study presented here aimed to investigate the antioxidant effect of vitamin C on antioxidant enzyme activities and lipid peroxidation in rainbow trout exposed to subchronic dosage diazinon. A total number of 360 Oncorhynchus mykiss were allocated into four treatment groups (with three replicates): control; diazinon (0.1 mg L 1); vitamin C (300 mg kg 1 in diet) plus diazinon (0.1 mg L-1), and vitamin C (1000 mg kg-1 in diet) plus diazinon (0.1 mg L-1). After two and four weeks, blood samples were collected and superoxide dismutase, catalase enzymes, total antioxidant capacity and malondialdehyte index were measured. The specimens exposed exclusively to diazinon showed a significant decrease in superoxide dismutase and total antioxidant capacity activities and also an increase in catalase activity and malondialdehyde index compared to those of the control group. The changes in oxidative stress parameters may be related to destructive free oxiradicals such as superoxide and hydroxyl radicals produced in diazinon metabolism. In conclusion, vitamin C moderated activity of the antioxidant enzyme, increased the total antioxidant capacity, and decreased cellular damages caused by destructive lipid peroxidation process in presence of diazinon.
|Publication Date||May 15, 2016|
|Application Date||May 15, 2016|
|Published in Issue||Year 2016, Volume 33, Issue 2|