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İçme suyu kaynağı ve musluk suyunda metal(loid)lerin varlığına bağlı genotoksisite değerlendirmesi

Year 2025, Volume: 42 Issue: 1, 33 - 40, 08.03.2025

Seda Kontaş Yalçınkaya , Derya Bostancı , Serdar Yedıer

https://doi.org/10.12714/egejfas.42.1.05
Cited By: 1

Abstract

İçme suyu kaynaklarının doğal süreçler ve antropojenik aktivitelerle kirlenmesi nedeniyle içme suyu kaynakları ve musluk sularındaki metaloid ve ağır metal (metal(oid)lerin) konsantrasyonlarının ölçülmesi önem kazanmıştır. Bu çalışmada esas olarak içme suyu kaynağı (Kacalı Deresi) ve musluk suyunun (Perşembe İlçesi) genotoksisitesi comet analizi yöntemi ile araştırılmıştır. Metal(oid)lerin etkisi Cyprinus carpio’nun eritrosit hücreleri kullanılarak in vivo izlenmiştir. Su örneklerinde sekiz ağır metal (alüminyum, nikel, kadmiyum, kurşun, manganez, demir, bakır, çinko) ve bir metaloid (arsenik) bulunmuş ve toplam metal(oid) içerikleri mevsimsel olarak belirlenmiştir. Genel olarak Perşembe musluk suyunun toplam metal(oid) içeriği tüm mevsimlerde Kacalı Deresi'nden daha yüksek bulunmuştur. Özellikle yaz aylarında musluk suyu C. carpio eritrositlerinde daha fazla DNA hasarına neden olmuştur. Kacalı Nehri'nden alınan su örnekleri, tüm mevsimlerde kontrol gruplarıyla karşılaştırıldığında önemli ölçüde daha yüksek genotoksisite göstermiştir. İçme suyundaki genotoksisite ile ilişkili sağlık risklerini azaltmak için su kaynaklarının dikkatli bir şekilde yönetilmesi gerekmektedir.

Keywords

Comet analizi içme suyu DNA hasarı metal(loid) içeriği musluk suyu Cyprinus carpio

Project Number

G-2004

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Genotoxicity assessment on presence of metal(loid)s in drinking water source and tap water

Year 2025, Volume: 42 Issue: 1, 33 - 40, 08.03.2025

Seda Kontaş Yalçınkaya , Derya Bostancı , Serdar Yedıer

https://doi.org/10.12714/egejfas.42.1.05
Cited By: 1

Abstract

It is important to quantify the concentrations of metalloids and heavy metals (metal(oid)s) in drinking water sources and tap water due to contamination of drinking water sources by natural processes and anthropogenic activities. In this study, the genotoxicity of drinking water sources (Kacalı River) and tap water (Perşembe district) was mainly investigated by comet assay. The effect of metal(loid)s was monitored in vivo using erythrocyte cells of Cyprinus carpio. The eight heavy metals (aluminium, nickel, cadmium, lead, manganese, iron, copper, zinc) and one metalloid (arsenic) were found in the water samples and the total content of metal(loid)s was determined seasonally. In general, the total metal(loid)s content of the Perşembe tap water was higher than that of the Kacalı River in all seasons. Especially in summer, tap water causes higher DNA damage in C. carpio erythrocytes. Water samples from the Kacalı River showed significantly higher genotoxicity compared to control groups in all seasons. Careful management of water supplies is needed to reduce the health risks associated with genotoxicity in drinking water.

Keywords

Comet assay drinking water DNA damage metal(loid) content tap water Cyprinus carpio

Ethical Statement

All experimental procedures were approved by Animal Experiments Local Ethics Committee, Ordu University (Approval number: 82678388/5).

Supporting Institution

Ordu University Scientific Research Projects (SRP) Commission (Project Number G-2004)

Project Number

G-2004

Thanks

The authors thank the Scientific Research Project Fund of Ordu University. The authors also thank Prof. Dr. Nazmi Polat, Prof. Dr. Evren Tunca and Prof. Dr. Salih Alkan their insightful feedback on this paper.

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  • Altunkaynak, F., Çavuşoğlu, K., & Yalçin, E. (2023). Detection of heavy metal contamination in Batlama Stream (Türkiye) and the potential toxicity profile. Scientific Reports, 13, 11727. https://doi.org/10.1038/s41598-023-39050-4
  • Andrade, V.M., Freitas, T.R.O., & Silva, J. (2004). Comet assay using mullet (Mugil sp.) and sea catfish (Netuma sp.) erythrocytes for the detection of genotoxic pollutants in aquatic environment. Mutation Research, 560(1), 57–67. https://doi.org/10.1016/j.mrgentox.2004.02.006
  • Barbosa, J.S., Cabral, T.M., Ferreira, D.N., Agnez-Lima, L.F., & Batistuzzo de Medeirosa, S.R. (2010). Genotoxicity assessment in aquatic environment impacted by the presence of heavy metals. Ecotoxicology and Environmental Safety, 73, 320 325. https://doi.org/10.1016/j.ecoenv.2009.10.008
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  • Boucard, C.V., Lee-Cruz, L., Mercier, L., Ramírez Orozco, M., Serrano Pinto, V., Anguiano, G., Cazares, L., & Díaz, D. (2017). A study of DNA damage in buccal cells of consumers of well- and/or tap-water using the comet assay: Assessment of occupational exposure to genotoxicants. Environmental and Molecular Mutagenesis, 58(8), 619-627. https://doi.org/10.1002/em.22111
  • Bozzo, S., Azimonti, G., Villa, S., Di Guardo, A., & Finizio, A. (2013). Spatial and temporal trend of groundwater contamination from terbuthylazine and desethyl-terbuthylazine in the Lombardy Region (Italy). Environmental Science: Processes & Impacts, 15(2), 366 372. https://doi.org/10.1039/c2em30536d
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  • Ceretti, E., Moretti, M., Zerbini, I., Villarini, M., Zani, C., Monarca, S., & Feretti, D. (2016). Occurrence and control of genotoxins in drinking water: a monitoring proposal. Journal of Public Health Research, 5, 769. https://doi.org/10.4081/jphr.2016.769
  • Chakrabarty, S., & Sarma, H.P. (2011). Heavy metal contamination of drinking water in Kamrup district, Assam, India. Environmental Monitoring and Assessment, 179(1–4), 479-486. https://doi.org/10.1007/s10661-010-1750-7
  • Chatha, A.M.M., Naz, S., Iqbal, S.S., Kiran, A., Lateef, M., Zahra, U., Yasmin, F., Amjad, N., & Javaid, A. (2024). Detection of DNA damage in fish using comet assay. Current Trends in OMICS, 4(1), 01-16. https://doi.org/10.32350/cto.41.01
  • Cortés, C., & Marcos, R. (2018). Genotoxicity of disinfection byproducts and disinfected waters: A review of recent literature. Mutation Research: Genetic Toxicology and Environmental Mutagenesis, 831, 1-12. https://doi.org/10.1016/j.mrgentox.2018.04.005
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  • Dağ, N., & Arıcı, Ö. K. (2021). Heavy metals in soils Pb (lead), Hg (mercury), Cd (cadmium), As (arsenic) effects on human health. Uluslararası Çevresel Eğilimler Dergisi, 5(2), 48-59.
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There are 57 citations in total.

Details

Primary Language English
Subjects Hydrobiology
Journal Section Articles
Authors

Seda Kontaş Yalçınkaya 0000-0002-6582-6722

Derya Bostancı 0000-0003-3052-9805

Serdar Yedıer 0000-0003-0017-3502

Project Number G-2004
Publication Date March 8, 2025
Submission Date November 22, 2024
Acceptance Date February 3, 2025
Published in Issue Year 2025 Volume: 42 Issue: 1

Cite

APA Kontaş Yalçınkaya, S., Bostancı, D., & Yedıer, S. (2025). Genotoxicity assessment on presence of metal(loid)s in drinking water source and tap water. Ege Journal of Fisheries and Aquatic Sciences, 42(1), 33-40. https://doi.org/10.12714/egejfas.42.1.05

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