Determinations of the effects of cyfluthrin on the hemocytes parameters of freshwater mussel (Unio delicatus)

Pınar Arslan

TR EN

Tatlı su midyesinin (Unio delicatus) hemosit parametreleri üzerine Cyfluthrinin etkilerinin belirlenmesi

Öz

Pestisitlerin aşırı kullanımı 20. yüzyıldan beri artan bir şekilde devam etmektedir. Bu kullanıma bağlı olarak hedef organizmalar dışında hedef olmayan organizmalar da etkilenmektedir. Cyfluthrin tarımda, evsel alanda ve veteriner sağlığında böceklere karşı kullanılan bir sentetik piretroit pestisittir. Sucul ekosistemlere karışması sonucunda hedef dışı sucul organizmalar üzerinde de etki gösterebilir. Bu çalışmada, sucul omurgasız türlerinden biri olan tatlı su midyelerinde cyfluthrinin etkisi hemosit parametreleri ile incelenmesi amaçlanmıştır. 24 ve 48 saat süre ile farklı cyfluthrin maruziyetine bırakılan midyelerden alınan hemolemften total hemosit sayısı, hemolemf hücre morfolojisi ve diferansiyel hemosit sayımı yapılmıştır. Kontrol grubuna göre, deney gruplarının total hemosit sayıları önemli ölçüde değişkenlik gösterdiği bulunmuştur (p<0.05). Hemosit morfolojilerinin incelenmesinde ise granüllü, yarı-granüllü ve hiyalinosit hücreleri gözlenmiştir. Ardından yapılan diferansiyel hemosit sayımı ile 24 ve 48 saatlik maruziyet sonucunda benzer değerler bulunmuştur. Sonuç olarak, sucul toksikoloji çalışmalarında total hemosit sayısının yanı sıra hemosit morfolojilerinin incelenmesi ve diferansiyel hemosit sayıları da iyi bir biyobelirteçtir.

Anahtar Kelimeler

Thanks

The author would like to thank Prof. Dr. Mehmet Zeki Yıldırım for the identification of the mussel species and Prof. Dr. Aysel Çağlan Günal for kindly giving the cyfluthrin standard.

References

Alkan Uçkun, A. (2018). Investigation of toxic metal contamination in water and sediments of Gölbaşı Lake (Adıyaman). Adıyaman University Journal of Science, 8 (2), 129-140.
Arslan, P., Yurdakok-Dikmen, B., Kuzukiran, O., Ozeren, S.C. & Filazi, A. (2021a). Effects of acetamiprid and flumethrin on Unio sp. primary cells. Biologia, 76(4):1359-1365. DOI:10.1007/s11756-021-00692-2
Arslan, P., Yurdakok-Dikmen, B., Ozeren, S.C., Kuzukiran, O. & Filazi, A. (2021b). In vitro effects of erythromycin and florfenicol on primary cell lines of Unio crassus and Cyprinus carpio. Environmental Science and Pollution Research, DOI:10.1007/s11356-021-14139-3
Ayhan, M.M., Katalay, S. & Günal, A.Ç. (2021). How pollution effects the immune systems of invertebrate organisms (Mytilus galloprovincialis Lamark, 1819). Marine Pollution Bulletin, 172, 112750. DOI:10.1016/j.marpolbul.2021.112750
Banumathi, B., Vaseeharan, B., Suganya, P., Citarasu, T., Govindarajan, M., Alharbi, N.S., Kadaikunnan, S., Khaled, J.M. & Benelli, G. (2017). Toxicity of Camellia sinensis-fabricated silver nanoparticles on invertebrate and vertebrate organisms: morphological abnormalities and DNA damages. Journal of Cluster Science, 28, 2027–2040. DOI:10.1007/s10876-017-1201-5
Baussant, T., Bechmann, R. K., Taban, I. C., Larsen, B. K., Tandberg, A. H., Bjørnstad, A., Torgrimsen, S., Naevdal, A., Øysaed, K. B., Jonsson, G. & Sanni, S., (2009). Enzymatic and cellular responses in relation to body burden of PAHs in bivalve molluscs: a case study with chronic levels of North Sea and Barents Sea dispersed oil. Marine Pollution Bulletin, 58(12), 1796-1807. DOI:10.1016/j.marpolbul.2009.08.007
Berber, S., Ateş, S. & Acar, S., (2018). First observation of the zebra mussel (Dreissena polymorpha (Pallas, 1771)) on the narrow-clawed crayfish inhabiting in some water sources of Turkey. Ege Journal of Fisheries and Aquatic Sciences, 35(1), 55-61. DOI:10.12714/egejfas.2018.35.1.10
Bolognesi, C., & Fenech, M. (2012). Mussel micronucleus cytome assay. Nature protocols, 7(6), 1125-1137.

Bolotov, I.N., Kondakov, A.V., Konopleva, E.S., Vikhrev, I. V., Aksenova, O. A, Aksenov, A. S., Bespalaya, Y. V., Borovskoy, A. V., Danilov, P. P., Dvoryankin, G. A. Gofarov, M. Y., Kabakov, M. B., Klishko, O. K., Kolosova, Y. S., Lyubas, A. A., Novoselov, A. P., Palatov, D. M., Savvinov, G. N., Solomonov, N. M., Spitsyn, V. M., Sokolova, S. E., Tomilova, A. A., Froufe, E., Bogan, A. E., Lopes-Lima, M., Makhrov, A. A. & Vinarski, M. M., (2020). Integrative taxonomy, biogeography and conservation of freshwater mussels (Unionidae) in Russia. Scientific Reports, 10, 3072. DOI:10.1038/s41598-020-59867-7
Canesi, L., Gavioli, M., Pruzzo, C. & Gallo, G. (2002). Bacteriahemocyte interactions and phagocytosis in marine bivalves. Microscopy Research Technique, 57, 469- 476. DOI:10.1002/jemt.10100
Ciacci, C., Fabbri, R., Betti, M., Roch, P. & Canesi, L. (2009). Seasonal changes in functional parameters of the hemolymph of Mytilus galloprovincialis. Invertebrate Survival Journal, 6(1), 44-48.
Cryder, Z., Wolf, D., Carlan, C. & Gan, J. (2021). Removal of urban-use insecticides in a large-scale constructed wetland. Environmental Pollution, 268, Part A, 115586. DOI:10.1016/j.envpol.2020.115586
Costa, P.M., Carreira, S., Costa, M. H. & Caeiro, S. (2013). Development of histopathological indices in a commercial marine bivalve (Ruditapes decussatus) to determine environmental quality. Aquatic Toxicology, 126, 442-454. DOI:10.1016/j.aquatox.2012.08.013
Duaví, W.C., Gama, A.F., Damasceno, É.P., Moreira, L.B., A Da Silva, V.P., Nascimento, R.F. & Cavalcante, R.M. (2021) Are pesticides only a problem from rural areas? The case of a highly urbanised tropical mangrove (Fortaleza, CE, Brazil). International Journal of Environmental Analytical Chemistry. DOI:10.1080/03067319.2021.1946524
Farag, M.R., Alagawany, M., Bilal, R.M., Gewida, A.G.A., Dhama, K., Abdel-Latif, H.M.R., Amer, M.S., Rivero-Perez, N., Zaragoza-Bastida, A., Binnaser, Y.S., Batiha, G.E.-S. & Naiel, M.A.E. (2021). An overview on the potential hazards of pyrethroid insecticides in fish, with special emphasis on cypermethrin toxicity. Animals, 11(7): 1880. DOI:10.3390/ani11071880
Gillis, P.L., Higgins, S.K. & Jorge, M.B. (2014). Evidence of oxidative stress in wild freshwater mussels (Lasmigona costata) exposed to urban-derived contaminants. Ecotoxicology and Environmental Safety, 102, 62-69. DOI:10.1016/j.ecoenv.2013.12.026
Gustafson, L. L., Stoskopf, M. K., Bogan, A. E., Showers, W., Kwak, T. J., Hanlon, S. & Levine, J. F. (2005). Evaluation of a nonlethal technique for hemolymph collection in Elliptio complanata, a freshwater bivalve (Mollusca: Unionidae). Diseases of Aquatic Organisms, 65(2), 159-165. DOI:10.3354/dao065159
Günal, A.Ç., Erkmen, B., Katalay,S., Ayhan, M.M., Gül, G. & Erkoç, F. (2018). Determinations of the effects antifouling copper pyrithione on total hemocyte counts of mussel (Mytilus galloprovincialis). Ege Journal of Fisheries and Aquatic Sciences, 35(1): 15-17. DOI:10.12714/egejfas.2018.35.1.03
Günal, A.Ç., Tunca, S.K., Arslan, P., Gül, G. & Sepici Dinçel, A. (2021). How does sublethal permethrin effect non-target aquatic organisms?. Environmental Science and Pollution Research, 28, 52405–52417. DOI:10.1007/s11356-021-14475-4
Katalay, S., Ayhan, M.M. & Günal, A.Ç. (2019). The effects of zinc pyrithione on total hemocyte counts of mussel (Mytilus galloprovincialis Lamarck, 1819). Ege Journal of Fisheries and Aquatic Sciences, 36(2), 185-189. DOI:10.12714/egejfas.2019.36.2.11
Larguinho, M., Cordeiro, A., Diniz, M.S., Costa, P.M. & Baptista, P.V., (2014). Metabolic and histopathological alterations in the marine bivalve Mytilus galloprovincialis induced by chronic exposure to acrylamide, Environmental Research, 135, 55-62. DOI:10.1016/j.envres.2014.09.004
Lizotte, R.E., Steinriede, R.W. & Locke, M.A. (2021) Occurrence of agricultural pesticides in Mississippi Delta Bayou sediments and their effects on the amphipod: Hyalella azteca. Chemistry and Ecology, 37(4), 305-322. DOI:10.1080/02757540.2021.1886281
Lopes-Lima, M., Gürlek, M.E., Kebapçı, Ü., Şereflişan, H., Yanık, T., Mirzajan, A., Neubert, E., Prie, V., Teixeira, A., Gomes-dos-Santos, A., Barros-Garcia, D., Bolotov, I. N., Kondakov, A. V., Vikhrev, I. V., Tomilova, A. A., Özcan, T., Altun, A., Gonçalves, D. V., Bogan, A. E. & Froufe, E., (2021). Diversity, biogeography, evolutionary relationships, and conservation of Eastern Mediterranean freshwater mussels (Bivalvia: Unionidae), Molecular Phylogenetics and Evolution, 163, 107261. DOI:10.1016/j.ympev.2021.107261
Lundquist, S.P., Worthington, T.A. & Aldridge, D.C. (2019). Freshwater mussels as a tool for reconstructing climate history. Ecological Indicators, 101, 11-21. DOI:10.1016/j.ecolind.2018.12.048.
Machado, A.A.S., Wood, C.M., Bianchini, A. & Gillis, P. L., (2014). Responses of biomarkers in wild freshwater mussels chronically exposed to complex contaminant mixtures. Ecotoxicology, 23, 1345–1358. DOI:10.1007/s10646-014-1277-8
Matazzo, V. & Marin, M.G. (2010). First cytochemical study of haemocytes from the crab Carcinus aestuarii (Crustacea, Decapoda). European Journal of Histochemistry, 54, e(9). DOI:10.4081/ejh.2010.e9
Matoo, O.B., Lannig, G., Bock, C. & Sokolova, I.M. (2021). Temperature but not ocean acidification affects energy metabolism and enzyme activities in the blue mussel, Mytilus edulis. Ecology and Evolution, 11(7), 3366-3379.
Negishi, J.N., Nagayama, S., Kume, M., Sagawa, S., Kayaba, Y. & Yamanaka, Y. (2013). Unionoid mussels as an indicator of fish communities: A conceptual framework and empirical evidence, Ecological Indicators, 24, 127-137. DOI:10.1016/j.ecolind.2012.05.029
Noleto, K.S., de Oliveira, S.R.S., Lima, I.M.A., de Jesus, W.B., da Silva Castro, J., de Santana, T.C., de Lima Cardoso, R., Jorge, M.B., Santos, D.M.S., de Torres-Junior, J.R. & Neta, R.N.F.C. (2021). Biochemical and histological biomarkers in Crassostrea sp. (Bivalvia, Ostreidae) for environmental monitoring of a neotropical Estuarine Area (Sao Jose Bay, Northeastern Brazil). Bulletin of Environmental Contamination and Toxicology, 106(4), 614-621. DOI:10.1007/s00128-021-03149-z
Nunes, A., Larson, M., Fragoso Jr, C. R. & Hanson, H. (2021). Modeling the salinity dynamics of a choked coastal lagoon and its impact on the Sururu mussel (Mytella falcata) population. Regional Studies in Marine Science, 45, 101807.
Palmquist, K., Salatas J. & Fairbrother, A. (2012). Pyrethroid insecticides: use, environmental fate, and ecotoxicology, In F. Perveen (Ed.), Insecticides: Advances in integrated pest management. ISBN: 978-953-307-780-2, InTech. DOI:10.5772/29495
Pérez–Velasco, R., Manzano–Sarabia, M. & Hurtado–Oliva, M. Á. (2021). Effect of hypo–and hypersaline stress conditions on physiological, metabolic, and immune responses in the oyster Crassostrea corteziensis (Bivalvia: Ostreidae). Fish & Shellfish Immunology, 120, 252-260. DOI:10.1016/j.fsi.2021.11.033
Sáenz, M.E, Di Marzio, W.D. & Alberdi, J.S. (2012). Assessment of Cyfluthrin commercial formulation on growth, photosynthesis and catalase activity of green algae. Pesticide Biochemistry and Physiology, 104(1), 50-57. DOI:10.1016/j.pestbp.2012.07.001
Saidov, D.M. & Kosevich, I.A. (2021). Rehabilitation of Mytilus edulis larvae abnormalities induced by K2Cr2O7 in short-term experiments. Ecotoxicology, 30(6), 1242-1250. DOI:10.1007/s10646-021-02441-2.
Selvi, M., Sarıkaya, R., Erkoç, F. & Koçak, O. (2008). Acute toxicity of the cyfluthrin pesticide on guppy fish. Environmental Chemistry Letters. DOI:10.1007/s10311-008-0142-5
Serdar, O. (2021). Determination of the effect of cyfluthrin pesticide on zebra mussel (Dreissena polymorpha) by Some Antioxidant Enzyme Activities. Journal of Anatolian Environmental and Animal Sciences, 6(1), 77-83. DOI:10.35229/jaes.804479
Sepici-Dinçel, A., Karasu Benli, A.Ç., Selvi, M., Sarıkaya, R., Şahin, D., Özkul, I.A. & Erkoç, F. (2009). Sublethal cyfluthrin toxicity to carp (Cyprinus carpio L.) fingerlings: Biochemical, hematological, histopathological alterations. Ecotoxicology and Environmental Safety, 72(5), 1433-1439. DOI:10.1016/j.ecoenv.2009.01.008
Silva, J.E.B., Boleli, I.C. & Simoes, Z.L.P. (2002) Hemocyte types and total and differential counts in unparasitized and parasitized Anastrepha obliqua (Diptera, Tephritidae) larvae. Brazilian Journal of Biology, 62(4A), 689-699. DOI:10.1590/s1519-69842002000400017
Soderlund, D.M., Clark, J.M., Sheets, L.P., Mullin, L.S., Piccirillo, V.J., Sargent, D., Stevens, J.T. & Weiner, M.L. (2002) Mechanisms of pyrethroid neurotoxicity: implications for cumulative risk assessment. Toxicology, 171, 3-59. DOI:10.1016/s0300-483x(01)00569-8
Tresnakova, N., Günal, A.Ç., Kankılıç, G.B., Pacal, E., Tavsanoglu, U.N., Uyar, R. & Erkoc, F. (2020). Sub-lethal toxicities of zinc pyrithione, copper pyrithione alone and in combination to the indicator mussel species Unio crassus Philipsson, 1788 (Bivalvia, Unionidae). Chemıstry and Ecology, 36, 292-308. DOI:10.1080/02757540.2020.1735377
Verma, R., Rajawat, N.K., Awasthi, K.K., Syed, F., John, P.J. & Soni, I. (2021). ROS dependent neurotoxicity, genotoxicity & histopathological alterations triggered by β-cyfluthrin, a synthetic pyrethroid. Materials Today: Proceedings, 42, 1737-1743. DOI:10.1016/j.matpr.2020.10.959
Wagner, A. & Boman, J. (2004) Biomonitoring of trace elements in Vietnamese freshwater mussels. Spectrochimica Acta Part B: Atomic Spectroscopy, 59(8),1125-1132. DOI:10.1016/j.sab.2003.11.009
Williams M.T., Gutierrez A. & Vorhees C.V. (2018) Effects of acute exposure of permethrin in adult and developing Sprague-Dawley rats on acoustic startle response and brain and plasma concentrations. Toxicological Sciences, 165(2), 361–371. DOI:10.1093/toxsci/kfy142
Wu, F., Sokolov, E. P., Dellwig, O. & Sokolova, I. M. (2021). Season-dependent effects of ZnO nanoparticles and elevated temperature on bioenergetics of the blue mussel Mytilus edulis. Chemosphere, 263, 127780. DOI:10.1016/j.chemosphere.2020.127780
Yavuzcan H.Y. & Benli, A.Ç.K. (2004) Nitrite toxicity to crayfish, Astacus leptodactylus, the effects of sublethal nitrite exposure on hemolymph nitrite, total hemocyte counts, and hemolymph glucose. Ecotoxicology and Environmental Safety, 59(3):370–375. DOI:10.1016/j.ecoenv.2003.07.007 Yee‐Duarte, J. A., Ceballos‐Vázquez, B. P., Arellano‐Martínez, M., Camacho‐Mondragón, M. A. & Uría‐Galicia, E. (2018). Histopathological alterations in the gonad of Megapitaria squalida (Mollusca: Bivalvia) inhabiting a heavy metals polluted environment. Journal of Aquatic Animal Health, 30(2), 144-154.
Yurdakok-Dikmen, B., Arslan, P., Kuzukiran, O., Filazi, A. & Erkoc, F. (2018). Unio sp. primary cell culture potential in ecotoxicology research. Toxin Reviews. 37(1): 75–81. DOI:10.1080/15569543.2017.1331360

Details

Primary Language

English

Subjects

Environmental Sciences

Journal Section

Research Article

Authors

Pınar Arslan ^*
0000-0001-5910-2835
Türkiye

Publication Date

March 15, 2022

Submission Date

August 29, 2021

Acceptance Date

January 3, 2022

Published in Issue

Year 1970 Volume: 39 Number: 1

IZ

https://izlik.org/JA39XY43DS

Cite

RIS / Bibtex

APA

Arslan, P. (2022). Determinations of the effects of cyfluthrin on the hemocytes parameters of freshwater mussel (Unio delicatus). Ege Journal of Fisheries and Aquatic Sciences, 39(1), 39-45. https://izlik.org/JA39XY43DS

Determinations of the effects of cyfluthrin on the hemocytes parameters of freshwater mussel (Unio delicatus)

Tatlı su midyesinin (Unio delicatus) hemosit parametreleri üzerine Cyfluthrinin etkilerinin belirlenmesi

Öz

Anahtar Kelimeler