Çakalburnu Lagünü (İzmir) sedimentlerinde real-time PCR ile bakteri ve arke düzeylerinin değerlendirilmesi

Burcu Omuzbüken; Aslı Kaçar

doi:10.12714/egejfas.38.2.02

TR EN

Çakalburnu Lagünü (İzmir) sedimentlerinde real-time PCR ile bakteri ve arke düzeylerinin değerlendirilmesi

Öz

Kıyısal lagünler, bir veya daha fazla sayıda giriş ile su değişimine izin veren ve bir bariyer gibi denizden ayrılan sığ su kütleleridir. Bu kırılgan ekosistemler kendilerine özgü sediment yapılarına sahip olmaktadır. Büyük ölçüde bentik mikrobiyal döngü yoluyla yürütülen biyojeokimyasal süreçler; lagün ile bitişik kıyı bölgesi arasındaki ilişkiyi anlamak için oldukça önemlidir. Bu çalışma, İzmir Körfezi'nde bulunan ve 67 hektarlık bir alan kaplayan Çakalburnu Lagünü’nde gerçekleştirilmiştir. Çalışmanın amacını, lagün sedimentlerinde, farklı mikrobiyal toplulukların düzeylerinin belirlenmesi oluşturmaktadır. Araştırmada, lagünün 7 noktasından toplanan sediment örneklerinde, Genel Arke (ARC), Metanojenik Arke (MCRA), Anaerobik Metan Oksidasyonu yapan Arke (ANME 1, ANME 2a, ANME 2c), Genel Bakteri (BAC) ve Sülfat İndirgeyen Bakteri (SRB2) seviyelerinin tespitinde Real-Time qPCR analizleri gerçekleştirilmiştir. Çakalburnu Lagünü sedimentlerinde incelenen Genel Arke ve Genel Bakteri bollukların maksimum değerleri sırasıyla 2,66x10¹⁰ gen kopya sayısı/gr ve 3,89x10⁷ gen kopya sayısı/gr olarak belirlenmiş olup, bu çalışmada lagün sedimentlerinde arkeal bolluğun yoğun olduğu görülmüştür. Mikrobiyal çeşitliliğin karakterizasyonu, ekosistemin biyolojik temellerinin anlaşılması açısından önemlidir. Çalışmamızda sunulan veriler, lagünler gibi hassas ekosistemlerde, ekolojik ve mikrobiyolojik dengenin korunması ile biyojeokimyasal döngülerin belirlenmesine yönelik çalışmalara katkı sağlamaktadır. Gelecekteki çalışmalarda, mikrobiyal grupların seviyelerinin mevsimlere ve yıllara göre değişimlerinin izlenmesi yönünde çalışmalar yürütülecektir.

Anahtar Kelimeler

Assessment of bacteria and archaea levels in Çakalburnu Lagoon (İzmir) sediments by real-time PCR

Abstract

Coastal lagoons are shallow water masses, discredited from the marines as a barrier that permits water to change through one or more inputs. These fragile ecosystems have a specific type of sediments with their own characteristics. Biogeochemical processes, mostly intervened by the benthic microbial loop, are significant for understanding the relationships among the lagoon and the contiguous coastal partition. This study was conducted in the Çakalburnu Lagoon (İzmir) area, which is located at the Bay of İzmir and the area covers 67 hectares. The aim of the present study is to constitute of determining the number of different microbial communities in the lagoon sediments. We collected from lagoon sediments samples at 7 stations and we applied a Real-time qPCR assay to determine levels of archaea (ARC), methanogenic archaea (MCRA), anaerobic methane oxidation archaea (ANME 1, ANME 2a, ANME 2c), bacteria (BAC) and sulfate-reducing bacteria (SRB2) in the study. The amount of maximum abundance of archaeal and bacterial 16S rRNA gene in sediments are 2,66x10¹⁰ gene copy numbers/g and 3,89x10⁷ gene copy numbers/g, respectively. So, it was established that the archaeal abundance was intense in the lagoon sediments. The characterization of microbial diversity is significant for the comprehension of the biological fundamentals of the ecosystem. The data presented in our study contributes to the studies on preserving ecological and microbiological balance and determining biogeochemical cycles in sensitive ecosystems such as lagoons. The research will be conducted on studies to determine the abundance levels of seasonal and annual microbial groups in the future.

Keywords

References

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Details

Primary Language

Turkish

Subjects

Ecology

Journal Section

Research Article

Authors

Burcu Omuzbüken
0000-0002-6681-7174
Türkiye

Aslı Kaçar ^*
0000-0002-8705-3695
Türkiye

Publication Date

June 15, 2021

Submission Date

September 21, 2020

Acceptance Date

December 24, 2020

Published in Issue

Year 1970 Volume: 38 Number: 2

DOI

https://doi.org/10.12714/egejfas.38.2.02

IZ

https://izlik.org/JA29XD42ES

Cite

RIS / Bibtex

APA

Omuzbüken, B., & Kaçar, A. (2021). Çakalburnu Lagünü (İzmir) sedimentlerinde real-time PCR ile bakteri ve arke düzeylerinin değerlendirilmesi. Ege Journal of Fisheries and Aquatic Sciences, 38(2), 147-154. https://doi.org/10.12714/egejfas.38.2.02

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