Dioktil Adipat (DOA) 'ın Sparus aurata (Çipura) karaciğer ve solungaç histolojisi üzerine etkileri.

Melih Üreten; Sema İşisağ Üçüncü

EN TR

Dioktil Adipat (DOA) 'ın Sparus aurata (Çipura) karaciğer ve solungaç histolojisi üzerine etkileri.

Öz

Polimer üretim süreçlerinde ikincil plastikleştirici olarak çok yaygın kullanılan dioktil adipat (DOA) ’ın ekotoksikolojik etkileri ayrıntılı olarak araştırılmamıştır. Bu çalışmanın amacı 15 gün süreyle 250, 500 ve 750 ppm DOA’ya maruz bırakılan erkek çipura balıklarının (Sparus aurata) solungaç ve karaciğerlerindeki histopatolojik değişimlerin ışık mikroskobu ile belirlenmesidir. Kontrol grubuyla karşılaştırıldığında, deneme grubu örneklerinin solungaçlarında vakuolizasyon, merkezi venada genişleme ve çarpıcı füzyon olarak ayırt edilen önemli histopatolojik değişimler gözlenmiştir. Ayrıca solungacın epitel, pillar mukus ve mitokondri zengini hücrelerinde belirgin hipertrofi ve hiperplazi de not edilmiştir. DOA’ya maruz bırakılan balıkların karaciğerlerinin incelenmesi yağlanma, fibrozis ve nekrozis olarak bir dizi hepatosellüler değişim ortaya koymuştur. Kanamaya ek olarak parankimatik sinüzoidler ve hepatopankreasın merkezi venleri genişlemiştir. Çalışma, DOA etkisinin araştırılan her iki dokuda da çoklu histolojik değişimlere yol açtığını, üstelik bu değişimlerin hepsinin uygulanan kimyasalın artan konsantrasyonuyla bağlantılı olduğunu göstermektedir. Sonuçta DOA’nın bir sucul kirletici olarak daha fazla dikkate alınması gerektiği vurgulanmıştır

Anahtar Kelimeler

Sparus aurata, dioktil adipat, solungaç, karaciğer, ekotoksikoloji

References

Agius, C., Roberts, R.J., 2003. Melano-macrophage Centres and Their Role in Fish Pathology: Review, Journal of Fish Diseases, 26:499-509. doi: 10.1046/j.1365-2761.2003.00485.x
Akaishi, F.M., Silva, A.H.C., Jakobi, S.C.G., Eiras, S.D.R., St-Jean, S.D., Courtenay, S.C., Lima, E.F., Wagener, A.L.R., Scofield, A.L., Oliveira, R.C.A., 2004. Morphological and Neurotoxicological Findings in Tropical Freshwater Fish (Astyanax sp.) After Waterborne and Acute Exposure to Water Soluble Fraction (WSF) of Crude Oil. Archives of Environmental Contamination and Toxicology 46:244-253.
Anonymous, 1999. “Plastic Wrap” www.nsc.org/ehc/minute/em990517.htm (02.09.2008)
Anonymous, 2010. “Hepatic Pathology”, The University of Utah, http://library.med.utah.edu/WebPath/LIVEHTML/LIVER042.html (05.07.2010)
Ayaş, Z., Ekmekçi, G., Özmen, M., Yerli, S.V., 2007. Histopathological Changes in the Livers and Kidneys of Fish in Sariyar Reservoir, Turkey. Environmental doi: 10.1016/j.etap.2006.11.003 Pharmacology, 23:242-249.
Ayoola, S.O., Ajani, E.K., 2008. Histopathological Effects of Cypermethrin on Juvenile African Catfish (Clarias gariepinus). World Journal of Biological Research, 01:2.
Barlas, N., 1999. Histopathological Examination of Gill, Liver and Kidney Tissues of Carp (Cyprinus carpio L., 1758) Fish in the Upper Sakarya River Basin (in Turkish with English abstract). Turkish Journal of Veterinary and Animal Sciences, 23 (Ek Sayı 2):277-284.
Boran, H., Altınok, İ., Çapkın, E., 2010. Histopathological Changes Induced by Maneb and Carbaryl on Some Tissues of Rainbow Trout, Oncorhynchus doi: 10.1016/j.tice.2010.03.004 and Cell, 42:158-164.

Cengiz, E.İ., 2006. Gill and Kidney Histopathology in the Freshwater Fish (Cyprinus carpio) After Acute Exposure Deltamethrin. Environmental Toxicology and Pharmacology, 22(2):200-204. doi: 10.1016/j.etap.2006.03.006
Cengiz, E.İ., Ünlü, E., 2005. Sublethal Effect of Commercial Deltamethrin on the Structure of the Gill, Liver and Gut Tissues of Mosquitofish, Gambusia affinis, A Microscopic Study. Environmental Toxicology and Pharmacology, 21:246-253. doi: 10.1016/j.etap.2005.08.005
Çapkın, E., Terzi, E., Boran, H., Yandı, İ., Altınok, İ., 2010. Effects of Some Pesticides on the Vital Organs of Juvenile Rainbow Trout (Oncorhynchus mykiss). Tissue and Cell, 42(6):376–382. doi: 10.1016/j.tice.2010.10.001
De Meloa, G.C., Donattib, L., Rudnikia, C.A.M., Fanta, E., 2008. Hepatic Alterations in the Fish Rhamdia quelen Contaminated with Folidol 600. Ecotoxicology and Environmental Safety, 71:821-829. doi: 10.1016/j.ecoenv.2008.02.001
Diler, Ö., Özen, M.R., 2002. Histopathology of the Epidermal Papilloma in Cage Cultured Mirror Carp (Cyprinus carpio) in Eğirdir Lake (in Turkish with English abstract). Turkish Journal of Veterinary and Animal Sciences 26(6):1207-1211.
Dezfuli, B.S., Simoni, E., Rossi, R., Manera, M., 2000. Rodlet Cells and Other Inflammatory Cells of Phoxinus phoxinus Infected with Raphidascaris acus (Nematoda). Disease of Aquatic Organisms 43:61-69. doi: 10.3354/dao043061
Fanta, E., Rios, F.S., Romão, S., Vianna, A.C.C., Freiberger, S., 2003. Histopathology of the Fish Corydoras paleatus Contaminated with Sublethal Levels of Organophosphorus in Water and Food. Ecotoxicology and Environmental Safety, 54:119-130. doi: 10.1016/S0147-6513(02)00044-1
Felder, J.D., Adams, W.J., Saeger, V.W., 1986. Assessment of the Safety of Dioctyl Adipate in Freshwater Environments. Environmental Toxicology and Chemistry, 5:777-784. doi: 10.1002/etc.5620050809
Fouad, M.M.K., El Sayed, A.M., Mahdy, A.N., 1999, Migration of DINP and DOP Plasticisers from PVC Sheets into Food. Environmental Management and Health 10(5):297-302. doi: 10.1108/09566169910289577
Goulas, A.E., Kontominas, M.G., 1996. Migration of Dioctyl Adipate Plasticizer from Food-grade PVC Film into Chicken Meat Products: Effect of Gamma-radiation. Zeitschrift für Lebensmittel-Untersuchung und - Forschung A, 202(3):250-255. doi: 10.1007/BF01263549
Henry, T.R., Spitsbergen, J.M., Hornung, M.W., Abnet, C.C., Peterson, R.E., 1997. Early Life Stage Toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin in Zebrafish (Danio rerio). Toxicology and Applied Pharmacology, 142: 56–68. doi: 10.1006/taap.1996.8024
Hinton, D.E., Lauren, D.J., 1990. Liver Structural Alterations Accompanying Chronic Toxicity in Fishes: Potential Biomarkers of Exposure. In: Biomarkers of Environmental Contamination, J.F. MacCarthy, L.R. Shugart (Eds). Lewis Publishers, Boca Raton, Florida, p17-57.
Hinton, D.E., Segner, H., Au, D.W.T., Kullman, S.W., Hartman, R.C., 2008. Liver Toxicity. In: The Toxicology of Fishes, R.T. Di Giulio, D.E. Hinton (Eds). CRC Press Inc., Florida, pp. 328-352. doi: 10.1201/9780203647295.ch7
Indirabai, W.P.S., Tharani, G., Seetha, P., 2010. Impact of Sublethal Concentration of Endosulfan on Biochemicals and Histology of Organ Tissues of Freshwater Fish, Labeo rohita (Hamilton 1822). The Bioscan, 5(2): 215-218.
İsisağ Üçüncü, S., Önen, Ö., Ergen, G., Üreten, M., Boz, E., Seferoğlu, K., Gökçe, B., 2010a. The Effects of Dioctyl Adipate (DOA) on the Gill Histology of Labidochromis caeruleus Fryer, 1956 (Cichlidae, Teleostei) (in Turkish with English abstract). Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16(2):343-346.
İsisağ Üçüncü, S., Ergen, G., Önen, Ö., Kolbaşı Tekkan, B., Üreten, M., Boz, E., Seferoğlu, K., Gökçe, B., 2010b. The Effects of Dioctyl Adipate (DOA) on The Liver Histology of Labidochromis caeruleus Fryer, 1956 (Cichlidae, Teleostei) (in Turkish with English abstract). Kafkas Üniversitesi. Veteriner Fakültesi Dergisi, 16(Suppl- B):197–203.
Katalay, S., Tuğlu, İ., Minareci, E., 2007. Light Microscopic Investigation of the Possible Toxic Effect on the Gill of Black Goby (gobius niger) Living in Izmir Bay (in Turkish with English abstract). Ege Tıp Dergisi, 46(2):61 – 66.
Koehler, A., 2004. The Gender-specific Risk to Liver Toxicity and Cancer of Flounder (Platichthys flesus (L.)) at the German Wadden Sea coast. AquaticToxicology, 70(4):257-276. doi: 10.1016/j.aquatox.2004.07.002
Kranz, H., Dethlefsen, V., 1990. Liver Anomalies in Dab Limanda limanda from the Southern North Sea with Special Consideration Given to Neoplastic Lesions. Diseases of Aquatic Organisms, 9: 171–185. doi: 10.3354/dao009171
Miranda, A.L., Roche, H., Randi, M.A.F., Menezes, M.L., Oliveira Ribeiro, C.A., 2008. Bioaccumulation of Chlorinated Pesticides and PCBs in the Tropical Freshwater Fish Hoplias malabaricus: Histopathological, Physiological, and Immunological Findings. Environment International, 34:939–949. doi: 10.1016/j.envint.2008.02.004
Moeller, P.D.R., Morton, S.L., Mitchell, B.A., Sivertsen, S.K., Fairey, E.R, Mikulski, T.M., Glasgow, H., Deamer-Melia, N.J., Burkholder, J.M., Ramsdell, J.S., 2001. Current Progress in Isolation and Characterization of Toxins Isolated from Pfiesteria piscicida. Environmental Health Perspectives, 109:739-743. doi: 10.2307/3454921
Nero, V., Farwell, A., Lister, A., Van Der Kraak, G., Lee, L.E.J., Van Meer, T., MacKinnon, M.D., Dixon, D.G., 2006. Gill and Liver Histopathological Changes in Yellow Perch (Perca flavescens) and Goldfish (Carassius auratus) Exposed to Oil Sands Process-Affected Water. Ecotoxicology and Environmental Safety, 63:365-377. doi: 10.1016/j.ecoenv.2005.04.014
Olojo, E.A.A., Olurin, K.B., Mbaka, G., Oluwemimo, A.D., 2005. Histopathology of the Gill and Liver Tissues of the African Catfish Clarias gariepinus Exposed to Lead. African Journal of Biotechnology, 4(1):117- 122.
Pacheco, M., Santos, M.A., 2002. Biotransformation, Genotoxic and Histopathological Effects of Environmental Contaminants in European Eel (Anguilla anguilla L.). Ecotoxicology and Environmental Safety, 53:331–347. doi: 10.1016/S0147-6513(02)00017-9
Peters, N., Köhler, A., Kranz, H., 1987. Liver Pathology in Fishes from the Lower Elbe as a Consequence of Pollution. Disease of Aquatic Organisms, 2:87–97. doi: 10.3354/dao002087
Van Dyk, J.C., Pieterse, G.M., Van Vuren, J.H.J., 2007. Histological Changes in the Liver of Oreochromis mossambicus (Cichlidae) After Exposure to Cadmium and Zinc. Ecotoxicology and Environmental Safety, 66: 432-440. doi: 10.1016/j.ecoenv.2005.10.012
Velmurugan, B., Selvanayagam, M., Cengiz, E.İ., Ünlü, E., 2007. Histopathology of Lambda-cyhalothrin on Tissues (Gill, Kidney, Liver and Intestine) of Cirrhinus mrigala. Environmental Toxicology and Pharmacology, 24:286-291. doi: 10.1016/j.etap.2007.07.001
Vural, N., 1977. Toxicological Assessment of Plastic Food Containers Used in Turkey (in Turkish with English abstract). Ankara Eczacılık Fakültesi Dergisi, 7(2):163-181.
Weisman, J.L., Miller, D.L., 2006. Lipoid Liver Disease and Steatitis in Captive Sapphire Damsel Pomacentrus pavo. Acta Ichthyol Piscator, 36(2): 99–104.
Wolke, R.E., George, C.J., Blazer, V.S., 1985. Pigmented Macrophage Accumulations (mmc; pmb): Possible Monitors of Fish Health, Parasitology and Pathology of Marine Organisms of the Word Ocean. NOAA Technical. Report, 93-97.

Details

Primary Language

Turkish

Subjects

-

Journal Section

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Authors

Melih Üreten

Sema İşisağ Üçüncü

Publication Date

September 1, 2013

Submission Date

December 7, 2015

Acceptance Date

-

Published in Issue

Year 2013 Volume: 30 Number: 3

IZ

https://izlik.org/JA46LP33HU

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RIS / Bibtex

APA

Üreten, M., & Üçüncü, S. İ. (2013). Dioktil Adipat (DOA) ’ın Sparus aurata (Çipura) karaciğer ve solungaç histolojisi üzerine etkileri. Ege Journal of Fisheries and Aquatic Sciences, 30(3), 115-122. https://izlik.org/JA46LP33HU

Dioktil Adipat (DOA) 'ın Sparus aurata (Çipura) karaciğer ve solungaç histolojisi üzerine etkileri.

The effects of Dioctyl Adipate (DOA) on liver and gill histology of Sparus aurata ( Sea Bream).

Abstract

Keywords

Dioktil Adipat (DOA) 'ın Sparus aurata (Çipura) karaciğer ve solungaç histolojisi üzerine etkileri.

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

IZ

Cite