Determination of genetic structure in Barbus tauricus Kessler, 1877 populations inhabiting a few streams along the Black Sea Region (Turkey) inferred from mtDNA Cytochrome b gene sequence analysis

Melek Özpiçak; Nazmi Polat

doi:10.12714/egejfas.2019.36.1.01

TR EN

Karadeniz Bölgesi (Türkiye) boyunca bazı akarsularda yaşayan Barbus tauricus Kessler, 1877 populasyonlarının mtDNA Sitokrom b sekans analizleri ile genetik yapısının belirlenmesi

Öz

Bu çalışma Karadeniz Bölgesi’ndeki farklı alanlarda örneklenen Barbus tauricus populasyonları arasındaki türiçi varyasyonları ortaya çıkarmak için gerçekleştirilmiştir. Karadeniz Bölgesi’ndeki farklı içsulardan (Akçay (Samsun), Engiz Çayı (Samsun), Terme Çayı (Samsun) Karadere (Trabzon) and Değirmenağzı Deresi (Düzce)) toplamda 250 örnek yakalanmıştır. Tür içi varyasyonlar mtDNA cyt b sekansı kullanılarak saptanmıştır. Popülasyonların genetik yapıları moleküler varyans analizi (AMOVA) ile belirlenmiştir. Bu çalışmada GENBANK veri tabanından alınan Cyprinus carpio L., 1758 (DQ868874.1)(dış grup) ve diğer Barbus türleri karşılaştırmalı analizlerde kullanılmıştır. Cyt b gen bölgesinin 1141 baz çiftlik toplam sekansı ve 11 haplotip elde edilmiştir. AMOVA analizinden elde edilen moleküler veriler B.tauricus türiçi varyasyonun varlığını ve varyasyonun % 26.1 oranında gruplar arasında olduğunu ortaya çıkarmıştır. Tüm populasyonlardan elde edilen F_ST değeri populasyonlardaki genetik heterojeniteyi doğrulamaktadır (F_ST= 0.59310). Genetik analizler, B. tauricus populasyonlarının coğrafik olarak farklılıklar gösterdiğini ortaya çıkarmıştır.

Anahtar Kelimeler

Kırım bıyıklı balığı,genetik yapı,cyt b,Karadeniz,Türkiye

Determination of genetic structure in Barbus tauricus Kessler, 1877 populations inhabiting a few streams along the Black Sea Region (Turkey) inferred from mtDNA Cytochrome b gene sequence analysis

Abstract

This study was carried out in order to determine the intras-pecies variation in Barbus tauricus populations sampled from different localities in the Black Sea Region. A total of 250 B. tauricus samples were collected from inland waters (Akçay Stream (Samsun), Engiz Stream (Samsun), Terme Stream (Samsun) Karadere Stream (Trabzon) and Değirmenağzı Stream (Düzce)) of Black Sea Region. Intra-species variations in the species of B. tauricus were determined using mitochondrial DNA cyt b squencing. The population genetic structure was revealed by analyzing the molecular variance (AMOVA). For this study, a member of the related fish group, Cyprinus carpio L., 1758 (DQ868874.1)(as an outgroup) and other Barbus species from the GENBANK database was used in comparative analyses. Therefore, total sequence of 1141 bp of the cyt b gene region was obtained and 11 haplotypes were determined. The molecular data obtained by AMOVA revealed that there were intraspecific variations of B. tauricus and that 26.61% of the variation were among the populations. The F_ST value estimated for all populations and loci confirms genetic heterogeneity in B.tauricus populations (F_ST= 0.59310). Genetic analyses revealed that B. tauricus populations are geographically structured.

Keywords

Crimean barbel,genetic structure,cyt b,Black Sea,Turkey

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Melek Özpiçak ^*
0000-0003-3506-4242
Türkiye

Nazmi Polat
0000-0001-9785-9927
Türkiye

Publication Date

March 15, 2019

Submission Date

April 17, 2018

Acceptance Date

October 12, 2018

Published in Issue

Year 2019 Volume: 36 Number: 1

DOI

https://doi.org/10.12714/egejfas.2019.36.1.01

IZ

https://izlik.org/JA88UW86XJ

Cite

RIS / Bibtex

APA

Özpiçak, M., & Polat, N. (2019). Determination of genetic structure in Barbus tauricus Kessler, 1877 populations inhabiting a few streams along the Black Sea Region (Turkey) inferred from mtDNA Cytochrome b gene sequence analysis. Ege Journal of Fisheries and Aquatic Sciences, 36(1), 1-11. https://doi.org/10.12714/egejfas.2019.36.1.01

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