Effects of Perfluorooctane Sulfonate Compounds on the Biochemical Activities in Mussels (Mytilus galloprovincialis)

Gizem Gülsever; Hatice Parlak

doi:10.12714/egejfas.2018.35.4.07

TR EN

Effects of Perfluorooctane Sulfonate Compounds on the Biochemical Activities in Mussels (Mytilus galloprovincialis)

Abstract

Our environment has been affected by increasing level of discharged organic and inorganic pollutants from anthropogenic sources. Perfluorinated compounds (PFCs) are one of the important sources of pollution and they are major risks for the aquatic ecosystems. The aim of this study is to determine the effects of PFOS on GST enzyme activities in mussels (Mytilus galloprovincialis). For this purpose, mussels were exposed to six different PFOS concentrations and the effects were evaluated. PFOS has been caused to a statistically significant increase in GST activity in hepatopancreas in all experimental groups compared with the control group. In conclusion, it has been approved that GST which is a defense mechanism of organisms, can be a very useful tool to detect the toxic effects of pollutants.

Keywords

Perfluorinated compounds,perfluorooctane sulfonate,mussels,Mytilus galloprovincialis,GST enzyme activity

References

Adams, S.M. (2002). Biological indicators of aquatic ecosystem stress. Bethesda, Maryland, USA: American Fisheries Society.
Ahrens, L. & Bundschuh, M. (2014). Fate and effects of poly- and perfluoroalkyl substances in the aquatic environment: A review. Environ. Toxicol. Chem. 33, 1921–1929. DOI: 10.1002/etc.2663
Barhoumi, B., Le Menach K., Clérandeau, C., Ameur, W.B., Budzinski.H., Driss,M.R. & Cachot J. (2014). Assessment of pollution in the Bizerte lagoon (Tunisia) by the combined use of chemical and biochemical markers in mussels, Mytilus galloprovincialis. Mar Pollut Bull., 15, 84(1-2), 379-90. DOI: 10.1016/j.marpolbul.2014.05.002
Boudreau, T. M., Sibley, P. K., Mabury, S. A., Muir, D. G. C., & Solomon, K. R. (2003). Laboratory evaluation of the toxicity of perfluorooctane sulfonate (PFOS) on Selenastrum capricornutum, Chlorella vulgaris, Lemna gibba, Daphnia magna, and Daphnia pulicaria. Archives of Environmental Contamination and Toxicology, 44(3), 0307-0313. DOI: 10.1007/s00244-002-2102-6
Boyacıoğlu, M., Arslan, Ö. Ç., Parlak, H. & Karaaslan, M. A. (2007). Mutagenicity of nonylphenol and octylphenol using Salmonella Mutation assay. EU Journal of Fisheries & Aquatic Sciences, 24, 3-4.
Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2), 248-254. DOI: 10.1016/0003-2697(76)90527-3
Cheung, C. C. C., Siu, W. H. L., Richardson, B. J., De Luca-Abbott, S. B. & Lam, P.K.S. (2004). Antioxidant responses to benzo [a] pyrene and Aroclor 1254 exposure in the green-lipped mussel, Perna viridis. Environmental Pollution, 128(3), 393-403. DOI: 10.1016/j.envpol.2003.09.010
Cheung, C. C. C., Zheng, G. J., Lam, P. K. S.,& Richardson, B. J. (2002). Relationships between tissue concentrations of chlorinated hydrocarbons (polychlorinated biphenyls and chlorinated pesticides) and antioxidative responses of marine mussels, Perna viridis. Marine Pollution Bulletin, 45(1-12), 181-191. DOI: 10.1016/S0025-326X(01)00301-0

Cheung, C. C. C., Zheng, G. J., Li, A. M. Y., Richardson, B. J. & Lam, P. K. S. (2001). Relationships between tissue concentrations of polycyclic aromatic hydrocarbons and antioxidative responses of marine mussels, Perna viridis. Aquatic Toxicology, 52(3-4), 189-203. DOI: 10.1016/S0166-445X(00)00145-4
De Luca-Abbott, S. B., Richardson, B. J., McClellan, K. E., Zheng, G. J., Martin, M. & Lam, P.K. (2005). Field validation of antioxidant enzyme biomarkers in mussels (Perna viridis) and clams (Ruditapes philippinarum) transplanted in Hong Kong coastal waters. Marine Pollution Bulletin, 51(8), 694-707. DOI: 10.1016/j.marpolbul.2005.01.010
EPA, U. (2010). 2015 PFOA Stewardship Program. United States Environmental Protection Agency Homepage (2010) http://www. epa. gov/oppt/pfoa/pubs/stewardship.
Ferreira, M., Moradas-Ferreira, P. & Reis-Henriques, M. A. (2005). Oxidative stress biomarkers in two resident species, mullet (Mugil cephalus) and flounder (Platichthys flesus), from a polluted site in River Douro Estuary, Portugal. Aquatic Toxicology, 71(1), 39-48. DOI: 10.1016/j.aquatox.2004.10.009
Fitzpatrick, P.J., O’Halloran, J., Sheehan, D. & Walsh, A.R. (1997). Assessment of a glutathione S-transferase and related proteins in the gill and digestive gland of Mytilus edulis (L.), as potential organic pollution biomarkers. Biomarkers 2, 51– 56. DOI: 10.1080/135475097231977
Funes, V., Alhama, J., Navas, J. I., López-Barea, J. & Peinado, J. (2006). Ecotoxicological effects of metal pollution in two mollusc species from the Spanish South Atlantic littoral. Environmental Pollution, 139(2), 214-223. DOI:10.1016/j.envpol.2005.05.016
Gallagher, E. P., Stapleton, P. L., Slone, D. H., Schlenk, D. & Eaton, D. L. (1996). Channel catfish glutathione S-transferase isoenzyme activity toward (±)-anti-benzo [a] pyrene-trans-7, 8-dihydrodiol-9, 10-epoxide. Aquatic Toxicology, 34(2), 135-150. DOI: 10.1016/0166-445X(95)00037-5
Gowland, B. T. G., McIntosh, A. D., Davies, I. M., Moffat, C. F. & Webster, L. (2002). Implications from a field study regarding the relationship between polycyclic aromatic hydrocarbons and glutathione S-transferase activity in mussels. Marine Environmental Research, 54(3-5), 231-235. DOI: 10.1016/S0141-1136(02)00129-0
Gunduz, G., Parlak, H., Arslan, Ö. Ç., Boyacioglu, M. & Karaaslan, M. A. (2013). Embryotoxic effects of Perfluorooctane Sulfonate Compounds in sea urchin Paracentrotus lividus. Fresenius Environmental Bulletin, 22(1A), 171-177.
Habig, W.H., Pabst, M.J. & Jakoby, W.B. (1974). Glutathione S-transferases the first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 249(22), 7130-7139.
Han, J., Won, E. J., Lee, M. C., Seo, J. S., Lee, S. J. & Lee, J. S. (2015). Developmental retardation, reduced fecundity, and modulated expression of the defensome in the intertidal copepod Tigriopus japonicus exposed to BDE-47 and PFOS. Aquatic Toxicology, 165, 136-143. DOI: 10.1016/j.aquatox.2015.05.022
Houde, M., De Silva, A. O., Muir, D. C. & Letcher, R. J. (2011). Monitoring of perfluorinated compounds in aquatic biota: an updated review: PFCs in aquatic biota. Environmental Science & Technology, 45(19), 7962-7973. DOI: 10.1021/es104326w
Huang, H., Huang, C., Wang, L., Ye, X., Bai, C., Simonich, M. T., ... & Dong, Q. (2010). Toxicity, uptake kinetics and behavior assessment in zebrafish embryos following exposure to perfluorooctanesulphonicacid (PFOS). Aquatic Toxicology, 98(2), 139-147. DOI: 10.1016/j.aquatox.2010.02.003
Jemec, A., Drobne, D., Tišler, T. & Sepčić, K. (2010). Biochemical biomarkers in environmental studies—lessons learnt from enzymes catalase, glutathione S-transferase and cholinesterase in two crustacean species. Environmental Science and Pollution Research, 17(3), 571-581. DOI: 10.1007/s11356-009-0112-x
Jocelyne, H., Ross, N.W. & Moon, T.W. (2012). Glutathione, glutathione S-transferase, and glutathione conjugates, complementary markers of oxidative stress in aquatic biota. Environ Sci Pollut Res.,19, 2007–2023. DOI: 10.1007/s11356-012-0909-x
Karaaslan, M. A., Parlak, H., Arslan, O. C. & Boyacıoglu, M. (2012). The embryotoxic effects of beta blocker atenolol on sea urchin Arbacia lixula embryos. Fresenius Enviromental Bulletin, 21(11b), 3362-3364.
Katalay, S., Boyacioglu, M., Arslan, O. C., Parlak, H. & Karaaslan, M. A. (2012). Phytotoxicity of water and sediment from Nif Brook (Izmir, Turkey) on green algae Desmodesmus (= Scenedesmus) subspicatus. Ekoloji, 21(83), 25-31. DOI: 10.5053/ekoloji.2012.833
Kissa, E. (Ed.). (2001). Fluorinated surfactants and repellents (Vol. 97). CRC Press.
Kovarova, J., Marsalek, P., Blahova, J., Jurcikova, J., Kasikova, B. & Svobodova, Z. (2012). Occurrence of perfluoroalkyl substances in fish and water from the Svitava and Svratka Rivers, Czech Republic. Bulletin of Environmental Contamination and Toxicology, 88(3): 456-460. DOI: 10.1007/s00128-011-0484-8
Kwok, K. Y., Wang, X. H., Ya, M., Li, Y., Zhang, X. H., Yamashita, N., ... & Lam, P. K. (2015). Occurrence and distribution of conventional and new classes of per-and polyfluoroalkyl substances (PFASs) in the South China Sea. Journal of Hazardous Materials, 285, 389-397. DOI: 10.1016/j.jhazmat.2014.10.065
Lindstrom, A. B., Strynar, M. J. & Libelo, E. L. (2011). Polyfluorinated compounds: Past, present, and future. DOI: 10.1021/es2011622
Liu, C., Chang, V. W. & Gin, K. Y. (2014). Oxidative toxicity of perfluorinated chemicals in green mussel and bioaccumulation factor dependent quantitative structure–activity relationship. Environmental Toxicology and Chemistry, 33(10), 2323-2332. DOI: 10.1002/etc.2679
Liu, C., Gin, K. Y. & Chang, V. W. (2014). Multi-biomarker responses in green mussels exposed to PFCs: effects at molecular, cellular, and physiological levels. Environmental Science and Pollution Research, 21(4), 2785-2794. DOI:10.1007/s11356-013-2216-6
Livingstone, D. R. (1998). The fate of organic xenobiotics in aquatic ecosystems: quantitative and qualitative differences in biotransformation by invertebrates and fish. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 120(1), 43-49. DOI: 10.1016/S1095-6433(98)10008-9
Mainwaring, G. W., John, N. A. S. H., Davidson, M. & Green, T. (1996). Isolation of a mouse theta glutathione S-transferase active with methylene chloride. Biochemical Journal, 314(2), 445-448. DOI: 10.1042/bj3140445
Martínez-Gómez, C., Campillo, J. A., Benedicto, J., Fernández, B., Valdés, J., García, I. & Sánchez, F. (2006). Monitoring biomarkers in fish (Lepidorhombus boscii and Callionymus lyra) from the northern Iberian shelf after the Prestige oil spill. Marine Pollution Bulletin, 53(5-7), 305-314. DOI: 10.1016/j.marpolbul.2006.03.010
Meyer, U. A. (1996). Overview of enzymes of drug metabolism. Journal of Pharmacokinetics and Biopharmaceutics, 24(5), 449-459. DOI: 10.1007/BF02353473
Müller, C. E., Spiess, N., Gerecke, A. C., Scheringer, M. & Hungerbühler, K. (2011). Quantifying diffuse and point inputs of perfluoroalkyl acids in a nonindustrial river catchment. Environmental Science & Technology, 45(23), 9901-9909. DOI: 10.1021/es202140e
Naile, J. E., Khim, J. S., Wang, T., Chen, C., Luo, W., Kwon, B. O., ... & Giesy, J. P. (2010). Perfluorinated compounds in water, sediment, soil and biota from estuarine and coastal areas of Korea. Environmental Pollution, 158(5), 1237-1244. DOI: 10.1016/j. envpol.2010.01.023
O'Brien, J. M., Carew, A. C., Chu, S., Letcher, R. J. & Kennedy, S. W. (2009). Perfluorooctane sulfonate (PFOS) toxicity in domestic chicken (Gallus gallus domesticus) embryos in the absence of effects on peroxisome proliferator activated receptor alpha (PPARα)-regulated genes. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 149(4), 524-530. DOI: 10.1016/j.cbpc.2008.11.009
OECD. (2002). Co-operation on Existing Chemicals Hazard Assessment of Perfluorooctane Sulfonate (PFOS) and Its Salts. Environment Directorate. Joint meeting of the Chemicals Committee and the Working Party on Chemicals, Pesticides and Biotechnology.
Otto, D. M., & Moon, T. W. (1995). 3, 3’, 4, 4’‐Tetrachlorobiphenyl Effects on Antioxidant Enzymes and Glutathione Status in Different Tissues of Rainbow Trout. Basic & Clinical Pharmacology & Toxicology, 77(4), 281-287. DOI: 10.1111/j.1600-0773.1995.tb01028.x
Parlak, H., Arslan, O.Ç., Boyacıoğlu, M. & Karaaslan, M.A. (2011). Ekotoksikoloji ders kitabı. Ege Üniversitesi Su Ürünleri Fakültesi Yayınları No:79, Ders Kitabı Dizini No:39.
Paul, A. G.,Jones, K. C. & Sweetman, A. J. (2009). A first global production, emission, and environmental inventory for perfluorooctane sulfonate, Environ. Sci. Technol., 43, 386–392. DOI: 10.1021/es802216n
Peakall, D.B. & Walker, C.H. (1994). The role of biomarkers in environmental assessment (2). Vertebrates. Ecotoxicology, 3, 173–179. DOI: 10.1007/BF00117082
Power, A. & Sheehan, D. (1996). Seasonal variation in the antioxidant defence systems of gill and digestive gland of the blue mussel, Mytilus edulis. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 114(2), 99-103. DOI: 10.1016/0742-8413(96)00024-2
Prevedouros, K., Cousins, I. T., Buck, R. C. & Korzeniowski, S. H. (2006). Sources, fate and transport of perfluorocarboxylates. Environmental Science & Technology, 40(1): 32-44. DOI: 10.1021/es0512475
Rodriguez-Ariza, A., Peinado, J., Pueyo, C. & Lopez-Barea, J. (1993). Biochemical indicators of oxidative stress in fish from polluted littoral areas. Canadian Journal of Fisheries and Aquatic Sciences, 50(12), 2568-2573. DOI: 10.1139/f93-280
Wang, T., Wang, P., Meng, J., Liu, S., Lu, Y., Khim, J. S. & Giesy, J. P. (2015). A review of sources, multimedia distribution and health risks of perfluoroalkyl acids (PFAAs) in China. Chemosphere, 129, 87-99. DOI: 10.1016/j.chemosphere.2014.09.021
Winston, G. W., Moore, M. N., Kirchin, M. A. & Soverchia, C. (1996). Production of reactive oxygen species by hemocytes from the marine mussel, Mytilus edulis: lysosomal localization and effect of xenobiotics. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 113(2), 221-229. DOI: 10.1016/0742-8413(95)02091-8
Yamashita, N., Kannan, K., Taniyasu, S., Horii, Y., Petrick, G. & Gamo, T. (2005). A global survey of perfluorinated acids in oceans. Marine Pollution Bulletin, 51(8-12), 658-668. DOI: 10.1016/j.marpolbul.2005.04.026
Yeung, L. W., So, M. K., Jiang, G., Taniyasu, S., Yamashita, N., Song, M., ... & Lam, P. K. (2006). Perfluorooctanesulfonate and related fluorochemicals in human blood samples from China. Environmental Science & Technology, 40(3), 715-720. DOI: 10.1021/es052067y
Zhao, Z., Tang, J., Mi, L., Tian, C., Zhong, G., Zhang, G., ... & Sun, H. (2017). Perfluoroalkyl and polyfluoroalkyl substances in the lower atmosphere and surface waters of the Chinese Bohai Sea, Yellow Sea, and Yangtze River estuary. Science of the Total Environment, 599, 114-123. DOI: 10.1016/j.scitotenv.2017.04.147
Zhou, Q., Zhang, J., Fu, J., Shi, J. & Jiang, G. (2008). Biomonitoring: an appealing tool for assessment of metal pollution in the aquatic ecosystem. Analytica Chimica Acta, 606(2), 135-150. DOI: 10.1016/j.aca.2007.11.018

Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Gizem Gülsever
0000-0001-7526-6207
Türkiye

Hatice Parlak ^*
Ege Üniverstitesi
0000-0002-7623-9662
Türkiye

Publication Date

December 15, 2018

Submission Date

February 21, 2018

Acceptance Date

September 6, 2018

Published in Issue

Year 2018 Volume: 35 Number: 4

DOI

https://doi.org/10.12714/egejfas.2018.35.4.07

IZ

https://izlik.org/JA82HZ48ZL

Cite

RIS / Bibtex

APA

Gülsever, G., & Parlak, H. (2018). Effects of Perfluorooctane Sulfonate Compounds on the Biochemical Activities in Mussels (Mytilus galloprovincialis). Ege Journal of Fisheries and Aquatic Sciences, 35(4), 417-422. https://doi.org/10.12714/egejfas.2018.35.4.07

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Effects of Perfluorooctane Sulfonate Compounds on the Biochemical Activities in Mussels (Mytilus galloprovincialis)

Perflorooktan Sülfonat (PFOS)’ın Midyelerde (Mytilus galloprovincialis) Biyokimyasal Etkilerinin İncelenmesi

Öz

Anahtar Kelimeler

Effects of Perfluorooctane Sulfonate Compounds on the Biochemical Activities in Mussels (Mytilus galloprovincialis)

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Nanoplastics influence the perfluorooctane sulfonate (PFOS) mediated toxicity on marine mussel Perna viridis: Single and mixture exposure study

Evaluation of the research trends on perfluorinated compounds using bibliometric analysis: knowledge gap and future perspectives

The diving beetle, Cybister lateralimarginalis (De Geer, 1774), as a bioindicator for subcellular changes affected by heavy metal(loid) pollution in freshwater ecosystems

What do emerging PFAS tell us that the classic ones did not? Insights from in vitro assays