Identification of different oyster species using RAPD-PCR

Emel Özcan Gökçek; Aynur Lök; Bilge Karahan; Evrim Kurtay

doi:10.12714/egejfas.2017.34.1.04

EN TR

Identification of different oyster species using RAPD-PCR

Abstract

Rapid determination of the genetic differences between some oyster samples observed in the Aegean Sea that were assumed as an invasive species and domestic oyster (Ostrea edulis, Linnaeus 1758) using RAPD (Random Amplified Polymorphic DNA)- PCR (Polymerase Chain Reaction) markers was aimed in this study. In this regard, domestic species samples and the other samples collected from Altınoluk coasts which were morphologically different than Ostrea species and were showing similarities to Crassostrea genus were genetically indetified by using 8 RAPD profiles. Total 343 bands were observed in the study. Polymorphism rate was found higher in the samples that might belong to Crassostrea genus. 6 of all loci considered in this study were found highly identical for these species. Total of 16 species-spesific diagnostic bands: 11 bands for the invasive species and 5 bands for the domestic species, were determined.

Keywords

Genetic analysis,RAPD,marker,oyster,alien species

References

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Emel Özcan Gökçek
EGE UNIV
Türkiye

Aynur Lök

Bilge Karahan

Evrim Kurtay

Publication Date

February 21, 2017

Submission Date

October 4, 2016

Acceptance Date

November 22, 2016

Published in Issue

Year 2017 Volume: 34 Number: 1

DOI

https://doi.org/10.12714/egejfas.2017.34.1.04

IZ

https://izlik.org/JA75CH37KR

Cite

RIS / Bibtex

APA

Gökçek, E. Ö., Lök, A., Karahan, B., & Kurtay, E. (2017). Identification of different oyster species using RAPD-PCR. Ege Journal of Fisheries and Aquatic Sciences, 34(1), 25-30. https://doi.org/10.12714/egejfas.2017.34.1.04

Cited By

First molecular record of the alien species Pacific oyster (Crassostrea gigas, Thunberg 1793) in the Marmara Sea, Turkey

Marine Science and Technology Bulletin

https://doi.org/10.33714/masteb.668529

Identification of different oyster species using RAPD-PCR

Identification of different oyster species using RAPD-PCR

Abstract

Keywords

Farklı istiridye türlerinin tespitinde RAPD-PZR kullanımı

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

First molecular record of the alien species Pacific oyster (Crassostrea gigas, Thunberg 1793) in the Marmara Sea, Turkey