Investigation of combined effect of zeolite and mercury toxicity on some serum biochemical parameters of Oreochromis niloticus

Özgür Fırat; Arzu Şahin İnandı

doi:10.12714/egejfas.2016.33.3.09

EN TR

Investigation of combined effect of zeolite and mercury toxicity on some serum biochemical parameters of Oreochromis niloticus

Abstract

In the present work, serum biochemical parameters of Oreochromis niloticus were used to assess protective effects of zeolite (Zeo) on toxicity of mercury (Hg). For this purpose fish were exposed to 0.01 and 0.05 mg/L Hg and 0.01 mg/L Hg+0.01 g/L Zeo and 0.05 mg/L Hg+0.05 g/L Zeo for 4 and 21 days. Cortisol, glucose, total protein, sodium, chlorine and potassium levels were measured in blood serum. The statistically significant differences were determined in all analysed parameters of the fish exposed to mercury and mercury+zeolite mixtures. In the mercury alone, levels of cortisol and glucose elevated after the both exposure periods (P˂0.05). The exposures of mercury+zeolite mixtures increased the cortisol level at 4 days and glucose level at 21 days (P˂0.05). At the both exposure periods in the mercury+zeolite mixtures, it not determined significant change in the total protein levels (P˃0.05), while they decreased in higher concentrations of mercury alone (P˂0.05). In the exposure of mercury alone especially in its higher medium concentraion, levels of sodium and chlorine declined, while level of potassium elevated (P˂0.05). On the other hand it was not determined significant alterations in levels of all analysed ions in exposure of mercury and in combination with zeolite (P˃0.05). The results showed that zeolite partially or totally played a protective role against the toxic effect of Hg since the metabolites and ions levels of O. niloticus were higher in the mercury alone than mercury+zeolite mixture.

Keywords: Oreochromis niloticus, mercury, zeolite, biochemical parameters

Öz

Sunulan bu çalışmada civa (Hg) toksisitesi üzerine zeolitin (Zeo) koruyucu etkisini değerlendirmek için Oreochromis niloticus’un serumundaki biyokimyasal parametreler kullanılmıştır. Bu amaçla balıklar 0.01 ve 0.05 mg/L Hg ile 0.01 mg/L Hg+0.01 g/L Zeo ve 0.05 mg/L Hg+0.05 g/L Zeo karışımlarının etkisine 4 ve 21 günlük sürelerle bırakılmıştır. Elde edilen kan serumunda kortizol, glukoz, total protein, sodyum, klor ve potasyum düzeyleri otoanalizatör cihazlarla ölçülmüştür. Doğrudan civa ve civa+zeolit karışımlarının etkisinde incelenen serum parametrelerinde ortam derişimine ve etki süresine bağlı olarak önemli değişiklikler saptanmıştır. Civanın tek başına etkisinde her iki etkileşim süresi sonunda da kortizol ve glukoz düzeyleri kontrol grubuna göre anlamlı artış göstermiştir (P˂0.05). Bununla birlikte civa+zeolit karışımlarının etkisinde kortizol düzeyinde 4 günlük, glukoz düzeyinde ise 21 günlük etki süresi sonunda anlamlı bir artış belirlenmiştir (P˂0.05). Denenen her iki sürede de civa+zeolit karışımlarının etkisinde anlamlı bir değişim göstermeyen (P˃0.05) total protein düzeyi yüksek civa derişiminin etkisinde anlamlı bir azalış göstermiştir (P˂0.05). Civanın doğrudan etkisinde özellikle de yüksek ortam derişiminde sodyum ve klor düzeyleri azalırken; potasyum düzeyleri artmıştır (P˂0.05). Civanın zeolitle birlikte etkisinde ise analiz edilen tüm bu iyonların düzeylerinde önemli bir değişim belirlenmemiştir (P˃0.05). Araştırma sonuçları O. niloticus’un serum metabolit ve iyon düzeylerinde belirlenen değişikliklerin civa+zeolit karışımına oranla civanın tek başına etkisinde daha fazla olduğunu ve ortamda zeolit bulunduğunda civa toksisitesinin kısmen ya da tamamen düzeldiğini göstermektedir

Anahtar Kelimeler

Oreochromis niloticus, civa, zeolit, biyokimyasal parametreler

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Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Özgür Fırat

Arzu Şahin İnandı

Publication Date

August 15, 2016

Submission Date

August 12, 2016

Acceptance Date

-

Published in Issue

Year 2016 Volume: 33 Number: 3

DOI

https://doi.org/10.12714/egejfas.2016.33.3.09

IZ

https://izlik.org/JA44HP68KT

Cite

RIS / Bibtex

APA

Fırat, Ö., & İnandı, A. Ş. (2016). Investigation of combined effect of zeolite and mercury toxicity on some serum biochemical parameters of Oreochromis niloticus. Ege Journal of Fisheries and Aquatic Sciences, 33(3), 251-257. https://doi.org/10.12714/egejfas.2016.33.3.09

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