Population genetics of Pomatomus saltatrix (Linnaeus, 1766) in the seas of Türkiye based on microsatellite DNA

İlyas Kutlu; Zehra Duygu Düzgüneş; Şirin Firidin; Melike Alemdağ; Ayşe Cebeci; İbrahim Turan

doi:10.12714/egejfas.42.2.06

TR EN

Türkiye denizlerindeki Pomatomus saltatrix (Linnaeus, 1766)'in mikrosatelit DNA tabanlı popülasyon genetiği

Öz

Lüfer (Pomatomus saltatrix (Linnaeus, 1766)), ticari açıdan oldukça önemli ve göç eden bir yırtıcı balık türü olup, Türkiye kıyılarında bulunmaktadır. Buna rağmen, Türkiye'deki Lüfer popülasyonlarının genetik yapısı hakkında detaylı bir çalışma yapılmamıştır. Ülkelerin biyolojik çeşitliliğini korumak için doğal kaynaklarda bulunan genetik çeşitliliğin belirlenmesi gerekmektedir. Bu çalışmada, Türkiye'nin kıyı bölgelerindeki Lüfer popülasyon yapısını inceledik; Türkiye kıyılarındaki 14 bölgeden toplanan Lüfer örneklerini mikrosatelit DNA analizi yaparak tanımlamayı amaçladık. Mikrosatelit analizi için sekiz lokus (ELF 17, ELF 37, ELF 49, ELF 19, ELF 39, ELF 46, ELF 44, ELF 50) analiz edildi. Toplam 14 popülasyondan 433 örnek incelendi. Toplamda tüm popülasyonlarda ve tüm lokuslarda 207 alel ve 61 spesifik alel tanımlanmıştır. Gözlemlenen en yüksek (Ho) heterozigot değerler ELF 50 (Ho: 0,991) iken en düşük değer ELF 19'dur (Ho: 0,716). Beklenen en yüksek (He) heterozigot değerler ELF 39 (He: 0,952) iken en düşük değer ELF 50'dir (He: 0,518). Hardy-Weinberg analiz sonuçlarına göre tüm popülasyonlarda önemli bir sapma olduğu belirlenmiştir. Lüfer popülasyonları filogenetik soylarına göre temel koordinat analizi (PCoA) uygulanarak kümelendirildiğinde ilk üç eksen toplam genetik varyasyonun %94'ünü göstermektedir. En yüksek varyasyon değerleri ve öz değerler 3. eksende bulunmuştur. Analiz sonuçları incelendiğinde Mersin lüfer popülasyonunun diğer popülasyonlardan farklı olarak kümelendiği açıkça görülmektedir. Mantel testine göre genetik ve coğrafi mesafe arasında düşük bir korelasyon (R2 = 0,3061, P = 0,01) tespit edilmiştir. Sonuç olarak Tüm popülasyonlarda karışık bireylerin olduğu ve düşük genetik farklılaşma gözlenmiştir.

Anahtar Kelimeler

Population genetics of Pomatomus saltatrix (Linnaeus, 1766) in the seas of Türkiye based on microsatellite DNA

Abstract

Bluefish, (Pomatomus saltatrix (Linnaeus, 1766)) a commercially important and highly migratory predatory fish species, is found along the coasts of Türkiye. Despite it’s widespread presence along these coasts, there has been no detailed study on the genetic structure of bluefish populations in Türkiye. In order to protect the biological diversity of countries, the genetic diversity present in natural resources must be identified. In this study, we examined the population structure of bluefish in the coastal regions of Türkiye; We aimed to identify bluefish samples collected from 14 regions along Türkiye coast by analyzing microsatellit DNA. For the microsatellite analysis, eight loci (ELF 17, ELF 37, ELF 49, ELF 19, ELF 39, ELF 46, ELF 44, ELF 50) were analyzed. A total of 433 samples from 14 populations were studied. In total 207 alleles and 61 specific alleles were identified across all populations and all loci. The highest observed (Ho) heterozygous values are ELF 50 (Ho: 0,991) while the lowest value is ELF 19 (Ho: 0.716). The highest expected (He) heterozygous values are ELF 39 (He: 0.952) while the lowest value is ELF 50 (He: 0.518). According to the Hardy-Weinberg analysis results, it was determined that there was a significant deviation in all populations. When bluefish populations are clustered according to their phylogenetic lineages by applying principal coordinate analysis (PCoA), the first three axes show 94% of the total genetic variation. The highest variation values and eigenvalues were found on the 3rd axis. When the analysis results are examined, it is clearly seen that the Mersin bluefish population is clustered differently from other populations. According to the Mantel test, a low correlation (R2 = 0.3061, P = 0.01) was detected between genetic and geographical distance. Admixed individuals and low genetic differentiation were observed in all populations.

Keywords

Supporting Institution

The Republic of Türkiye Ministry of Agriculture and Forestry General Directorate of Agricultural Research and Policies Agricultural (Funding No: TAGEM/ HAYSÜD /Ü/20/A6/P3/1814).

Project Number

TAGEM/ HAYSÜD /Ü/20/A6/P3/1814).

Ethical Statement

All experiments were carried out considering the ethical rules of the authorities, with the approval coded as 325.04.02-12 by the Ethical Committee of Animal Experiments of Central Fisheries Research Institute.

Thanks

The authors would like to thank Assoc. Prof. Dr. iBRAHİM TURAN for his valuable contributions in writing the article

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Details

Primary Language

English

Subjects

Fish Physiology and Genetics

Journal Section

Research Article

Authors

İlyas Kutlu ^*
0000-0002-6679-8399
Türkiye

Zehra Duygu Düzgüneş
0000-0001-6243-4101
Türkiye

Şirin Firidin
0000-0002-2926-1576
Türkiye

Melike Alemdağ
0000-0001-7352-0574
Türkiye

Ayşe Cebeci
0000-0002-4288-8525
Türkiye

İbrahim Turan
0000-0003-3400-5494
Türkiye

Early Pub Date

June 14, 2025

Publication Date

June 15, 2025

Submission Date

December 25, 2024

Acceptance Date

April 7, 2025

Published in Issue

Year 2025 Volume: 42 Number: 2

DOI

https://doi.org/10.12714/egejfas.42.2.06

IZ

https://izlik.org/JA25TZ79RF

Cite

RIS / Bibtex

APA

Kutlu, İ., Düzgüneş, Z. D., Firidin, Ş., Alemdağ, M., Cebeci, A., & Turan, İ. (2025). Population genetics of Pomatomus saltatrix (Linnaeus, 1766) in the seas of Türkiye based on microsatellite DNA. Ege Journal of Fisheries and Aquatic Sciences, 42(2), 138-147. https://doi.org/10.12714/egejfas.42.2.06