Investigations on Paradiplozoon bliccae (Reichenbach-Klinke, 1961) (Monogenea: Diplozoidae) found in Capoeta aydinensis, an endemic fish in Türkiye, based on ecological, molecular and host related factor approaches

Nesrin Emre; Nurten Aydoğdu; Ali Aydogdu; Özgür Emiroğlu

doi:10.12714/egejfas.40.4.02

TR EN

Türkiye'nin endemik balıklarından Capoeta aydinensis' ten Paradiplozoon bliccae (Reichenbach-Klinke, 1961) (Monogenea: Diplozoidae)'nin ekolojik, moleküler ve konak ilişkili faktör yaklaşımıyla araştırmalar

Öz

Bu çalışma, Muğla (Türkiye) ili yakınlarındaki Köyceğiz Gölü' nde bulunan endemik Capoeta aydinensis Turan, Küçük, Kaya, Güçlü & Bektaş, 2017’in diplozoid parazitleri üzerine yapılmıştır. Araştırmanın amacı, Türkiye' nin farklı bir coğrafi bölgesinde daha önce keşfedilmemiş bir lokasyondaki endemik balık türü C. aydinensis' in diplozoid türlerinin varlığına ilişkin kayıtların geliştirilmesidir. Ekim 2019 – Temmuz 2020 tarihleri arasında araştırma alanından balık ağları kullanılarak toplam 187 C. aydinensis bireyi toplanmış ve diplozoid türlerinin varlığı açısından incelenmiştir. Ayrıntılı morfolojik tanımlamanın ardından yalnızca bir diplozoid türü, Paradiplozoon bliccae (Reichenbach-Klinke, 1961) tanımlanmıştır. Bu morfolojik tür tanımlamasını moleküler yöntemlerle doğrulamak için, konak balıktan alınan parazitin internal transcribed spacer 2 (ITS2)gen bölgelerinin nükleotid dizileri belirlenmiş ve Bayesian Inference (BI) algoritmaları kullanılarak filogenetik analizleri yapılmıştır. Elde edilen moleküler bulgulara göre diplozoid parazit türünün morfolojik olarak tanımlanması doğrulanmıştır. Diplozoid parazit dizileri ayrıca LT560257 erişim numarasıyla GenBank' ta saklanan Paradiplozoon bliccae dizileriyle de yüksek homoloji (%99.32) göstermiştir. İncelenen 187 balıktan 27'sinin toplam 117 P. bliccae ile 14.4%; 4.3; 0.6 sırasıyla enfeksiyon oranı, enfekte balık başına ortalama parazit sayısı ve incelenen balık başına ortalama parazit sayısı enfekte olduğu belirlenmiştir. İlave olarak, mevsime ve konakçı cinsiyetine göre enfeksiyon oranı ve enfekte balık başına ortalama parazit sayısı hesaplanmıştır. Enfeksiyon oranı, enfekte balık başına ortalama parazit sayısı ve incelenen balık başına ortalama parazit sayısı için en yüksek değerler yaz aylarında bulunmuştur. P. bliccae' nin enfekte balık başına ortalama parazit sayısı ve incelenen balık başına ortalama parazit sayısı erkeklerde daha yüksekken, enfeksiyon oranı dişilerde daha yüksek bulunmuştur. Bildiğimiz kadarıyla bu çalışma, Muğla ili Köyceğiz Gölü' nde C. aydinensis' in ilk ihtiyoparazitolojik araştırmasıdır. Ayrıca bu çalışma ile ilk kez bu lokalitedeki konak balıklardan elde edilen P. bliccae' nin sekans dizi verileri de GenBank'a raporlanmıştır. Böylece bu çalışma, bu parazit türünün Türkiye'deki konak yelpazesini üçe, yer sayısını ise ikiye çıkarmıştır.

Anahtar Kelimeler

Investigations on Paradiplozoon bliccae (Reichenbach-Klinke, 1961) (Monogenea: Diplozoidae) found in Capoeta aydinensis, an endemic fish in Türkiye, based on ecological, molecular and host related factor approaches

Abstract

The present study investigated diplozoid parasites in an endemic species, Capoeta aydinensis Turan, Küçük, Kaya, Güçlü & Bektaş, 2017 in Köyceğiz Lake, near Muğla province (Türkiye). The aim of this research is to improve a record of diplozoid species occurrence in C. aydinensis, an endemic fish species by collecting data from a previously unexplored locality situated in a different geographical region of Türkiye. A total of 187 individuals of C. aydinensis were collected by using fishing nets from October 2019 to July 2020 and examined for the presence of diplozoid species. Only one species of diplozoid has been recorded, known as Paradiplozoon bliccae (Reichenbach-Klinke, 1961), which has been identified morphologically and confirmed through molecular analysis. The nucleotide sequences of the parasite's nuclear internal transcribed spacer (ITS2) gene marker were determined as well as phylogenetic analyses by using Bayesian inference (BI) analyses. On the basis of the molecular findings, the morphological identification of the diplozoid parasite species was confirmed. Of 187 fish sampled, 27 were infected with 117 P. bliccae, representing an abundance of 0.6, a mean intensity of 4.3 and a prevalence of 14.4%. The prevalence and mean intensity of infection were based on the season and sex of the host. The highest values of infection for prevalence, mean intensity and abundance were found in summer. Meanwhile, mean intensity and abundance of P. bliccae were higher in males, the prevalence was higher in females. To our knowledge, the present study is the first ichthyoparasitological study of C. aydinensis in Köyceğiz lake, near the province of Muğla in Türkiye. Furthermore, sequence data of P. bliccae from fish hosts in this locality were reported to GenBank for the first time as part of this study. Therefore, this study widens the host range of this parasite species in Türkiye.

Keywords

Supporting Institution

Akdeniz University of Scientific Research Project Unit

Project Number

FBA 2018-4175

Thanks

This study was supported by Akdeniz University of Scientific Research Project Unit, FBA 2018-4175. We also thank Dr. Hasan Emre Yılmaz from Akdeniz University, for helping with Bayesian Inference-based molecular analysis and phylogenetic tree. We also thank Fatma Messaoudene from the University of Djelfa, for helping with reading the manuscript.

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Details

Primary Language

English

Subjects

Freshwater Ecology

Journal Section

Research Article

Authors

Nesrin Emre
0000-0001-9047-1823
Türkiye

Nurten Aydoğdu ^*
0000-0002-9926-166X
Türkiye

Ali Aydogdu
0000-0003-2778-0273
Türkiye

Özgür Emiroğlu
0000-0002-4433-4286
Türkiye

Early Pub Date

December 8, 2023

Publication Date

December 15, 2023

Submission Date

August 11, 2023

Acceptance Date

October 18, 2023

Published in Issue

Year 1970 Volume: 40 Number: 4

DOI

https://doi.org/10.12714/egejfas.40.4.02

IZ

https://izlik.org/JA78YW77FL

Cite

RIS / Bibtex

APA

Emre, N., Aydoğdu, N., Aydogdu, A., & Emiroğlu, Ö. (2023). Investigations on Paradiplozoon bliccae (Reichenbach-Klinke, 1961) (Monogenea: Diplozoidae) found in Capoeta aydinensis, an endemic fish in Türkiye, based on ecological, molecular and host related factor approaches. Ege Journal of Fisheries and Aquatic Sciences, 40(4), 244-250. https://doi.org/10.12714/egejfas.40.4.02

Cited By

Antalya Bölgesi'nden Örneklenen Siraz (Capoeta antalyensis) Balıklarında Ektoparazit Faunasının Belirlenmesi

Journal of Limnology and Freshwater Fisheries Research

https://doi.org/10.17216/limnofish.1811542