A mutagenicity investigation of sediment from İzmir Inner Bay using Ames genotoxicity assay

Yiğit Egüz; Meltem Boyacıoğlu

doi:10.12714/egejfas.40.3.08

EN TR

A mutagenicity investigation of sediment from İzmir Inner Bay using Ames genotoxicity assay

Abstract

İzmir Bay is one of the most important ecosystems of Aegean Region. Impacts of environmental pollution in the aquatic environments, especially impacts of pollution with mutagenic and carcinogenic substances on human health is an important area of research. Thus, it is required to incorporate short-term biological research methods to the molecular chemical analysis methods. By means of Ames’s assay, it is possible to determine mutagenic potential of several chemicals, environmental pollutants, sediments, and waste waters. After the treatment facility was established in İzmir Bay in 2000, no mutagenicity studies were carried out in the sediment. Ames’s mutagenicity assay without S9 fraction using TA98 and TA100 strains of Salmonella typhimurium was done at four different concentrations (125 μg, 250 μg, 375 μg, and 500 μg) in the sediment samples from six stations on İzmir Inner Bay in order to detect presence of chemicals that may cause mutagenic effects. Based on the results of Ames’s assay, it was found that especially Turan Area (Station 3, on which shipyard is located) among 6 stations on İzmir Bay was under mutagenic and toxic effect and Bostanlı Area (Station 6) was under intense toxic effect. Turan Area was under the influence of environmental pollutants that may cause frameshift mutations. According to the present study, İzmir Inner Bay was contaminated by mutagenic and toxic substances.

Keywords

İzmir İç Körfezi sedimentinin Ames genotoksisite testi kullanılarak mutajenitesinin incelenmesi

Öz

İzmir Körfezi, Ege Bölgesi'nin en önemli ekosistemlerinden biridir. Su ortamlarında çevre kirliliğinin etkileri, özellikle mutajenik ve kanserojen maddelerle oluşan kirliliğin insan sağlığı üzerindeki etkileri önemli bir araştırma konusudur. Bu nedenle kısa süreli biyolojik araştırma yöntemlerinin moleküler kimyasal analiz yöntemleriyle birleştirilmesi gerekmektedir. Ames testi ile çeşitli kimyasalların, çevresel kirleticilerin, sediment ve atık suların mutajenik potansiyelini belirlemek mümkündür. İzmir Körfezi’nde 2000 yılında arıtma tesisi kurulduktan sonra sedimentte mutajenite çalışması yapılmamıştır. Bu amaçla İç Körfez'deki altı istasyondan alınan sediment örneklerinde, mutajenik etki potansiyeline sahip kimyasal maddelerin olup olmadığını tespit etmek için dört farklı konsantrasyonda (125 μg, 250 μg, 375 μg ve 500 μg) Salmonella typhimurium TA98 ve TA100 suşları kullanılarak S9 fraksiyonu olmadan Ames'in mutajenite tarama testi yapıldı. Ames testinin sonuçlarına göre, İzmir Körfezi'ndeki altı istasyondan özellikle Turan Bölgesi'nin (tersanenin bulunduğu İstasyon 3) mutajenik ve toksik etki altında olduğu, Bostanlı Bölgesi'nin (İstasyon 6) ise yoğun toksik etki altında olduğu belirlendi. Turan Bölgesi, çerçeve kayması mutasyonlarına sebep olabilen çevresel kirleticilerin etkisi altındadır. Bu çalışmaya göre, İzmir İç Körfezi mutajenik ve toksik etkili maddelerle kirlenmiştir.

Anahtar Kelimeler

Supporting Institution

Coordination Board for Scientific Research Projects of Ege University (FGA-2019-20553)

Project Number

FGA-2019-20553

Ethical Statement

No specific ethical approval was necessary for this study.

Thanks

The present study was supported with project numbered FGA-2019-20553 by the Coordination Board for Scientific Research Projects of Ege University. We would like to thank them for their contribution.

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Details

Primary Language

English

Subjects

Environmental Sciences

Journal Section

Research Article

Authors

Yiğit Egüz
0000-0002-2237-8202
Türkiye

Meltem Boyacıoğlu ^*
0000-0002-9871-6132
Türkiye

Early Pub Date

September 15, 2023

Publication Date

September 15, 2023

Submission Date

March 15, 2023

Acceptance Date

August 31, 2023

Published in Issue

Year 2023 Volume: 40 Number: 3

DOI

https://doi.org/10.12714/egejfas.40.3.08

IZ

https://izlik.org/JA68WA25MS

Cite

RIS / Bibtex

APA

Egüz, Y., & Boyacıoğlu, M. (2023). A mutagenicity investigation of sediment from İzmir Inner Bay using Ames genotoxicity assay. Ege Journal of Fisheries and Aquatic Sciences, 40(3), 211-218. https://doi.org/10.12714/egejfas.40.3.08

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